Separation of lymphocytes by electrophoresis under terrestrial conditions and at zero gravity, phase 3

Electrophoretic mobilities (EPM) of peripheral lymphocytes were studied from normal subjects, chronic hemodialysis patients and kidney transplant recipients. A technique to separate B lymphocytes and null cells from non-T lymphocyte preparation was developed. The experiments were designed to determine which subpopulation of the non-T lymphocytes is primarily affected and shows a decreased EPM in chronic hemodialysis patients and kidney transplant recipients

The urban economic development impacts of ethnic entrepreneurship : a case study of Dominican entrepreneurs in Lawrence, Massachusetts

Thesis (M.C.P.)--Massachusetts Institute of Technology, Dept. of Urban Studies and Planning, 2005.Includes bibliographical references (p. 109-114).This thesis examines the process through which ethnic entrepreneurship impacts urban economic development. In many urban places across the United States, demographic change has led to the rise of ethnic communities and the rise of ethnic entrepreneurs. In cities with sustained economic decline, the rise of ethnic entrepreneurship has been viewed with two perspectives: they are seen as symbols of continued economic decline or they are perceived as the new source of economic opportunity. Economic development practice has generally assumed the former, and in this thesis I focus on how ethnic entrepreneurship represents the latter. The thesis thus provides a strong criticism of contemporary economic development practice, and presents the case that economic development practice must embrace the role that ethnic entrepreneurship and the process of ethnic community formation plays in urban economic development. I present my arguments by first establishing a series of conceptual frameworks that show the ways in which ethnic entrepreneurship and the process of ethnic community formation influence economic activity and lead to urban economic development. I then apply the conceptual framework to a case study of Dominican entrepreneurs in the city of Lawrence, Massachusetts, a low-income city whose population is 61% Latino and 24% Dominican. Based on the findings and analysis from the case study showing the urban economic development impacts of Dominican entrepreneurship in Lawrence, I present a series of recommendations for economic development practice in the city that highlights the opportunities present in ethnic entrepreneurship in Lawrence.by Brian Chaneung Cheigh.M.C.P

VC-Dimension of Hyperplanes over Finite Fields

Let $\mathbb{F}_q^d$ be the $d$-dimensional vector space over the finite field with $q$ elements. For a subset $E\subseteq \mathbb{F}_q^d$ and a fixed nonzero $t\in \mathbb{F}_q$, let $\mathcal{H}_t(E)=\{h_y: y\in E\}$, where $h_y$ is the indicator function of the set $\{x\in E: x\cdot y=t\}$. Two of the authors, with Maxwell Sun, showed in the case $d=3$ that if $|E|\geq Cq^{\frac{11}{4}}$ and $q$ is sufficiently large, then the VC-dimension of $\mathcal{H}_t(E)$ is 3. In this paper, we generalize the result to arbitrary dimension and improve the exponent in the case $d=3$.Comment: 9 pages, 1 figur

Microbial Ecology of Watery Kimchi

© 2015 Institute of Food Technologists®. This article has been contributed by US Government employees and their work is in the public domain in the USA. The biochemistry and microbial ecology of 2 similar types of watery (mul) kimchi, containing sliced and unsliced radish and vegetables (nabak and dongchimi, respectively), were investigated. Samples from kimchi were fermented at 4, 10, and 20 °C were analyzed by plating on differential and selective media, high-performance liquid chromatography, and high-throughput DNA sequencing of 16S rDNA. Nabak kimchi showed similar trends as dongchimi, with increasing lactic and acetic acids and decreasing pH for each temperature, but differences in microbiota were apparent. Interestingly, bacteria from the Proteobacterium phylum, including Enterobacteriaceae, decreased more rapidly during fermentation at 4 °C in nabak cabbage fermentations compared with dongchimi. Although changes for Proteobacterium and Enterobacteriaceae populations were similar during fermentation at 10 and 20 °C, the homolactic stage of fermentation did not develop for the 4 and 10 °C samples of both nabak and dongchimi during the experiment. These data show the differences in biochemistry and microbial ecology that can result from preparation method and fermentation conditions of the kimchi, which may impact safety (Enterobacteriaceae populations may include pathogenic bacteria) and quality (homolactic fermentation can be undesirable, if too much acid is produced) of the product. In addition, the data also illustrate the need for improved methods for identifying and differentiating closely related lactic acid bacteria species using high-throughput sequencing methods.This work was carried out as part of the international collaborative R&D program funded by the Agency for Korea Natl. Food Cluster (2013), and supported in part by a grant from Pickle Packers Intl. Inc., Washington, D.C., U.S.A. The authors thank the Spanish Government (MECD) for the postdoctoral fellowship support for Dr. E. Medina-Pradas.Peer Reviewe

Fermentation by Lactobacillus enhances anti-inflammatory effect of Oyaksungisan on LPS-stimulated RAW 264.7 mouse macrophage cells

<p>Abstract</p> <p>Background</p> <p>Oyaksungisan (OY) has been used as a traditional drug in east-Asian countries. However, its effect on inflammation still remains unknown. In this study, to provide insight into the biological effects of OY and OY fermented by <it>Lactobacillus</it>, we investigated their effects on lipopolysaccharide (LPS)-mediated inflammation in the RAW 264.7 murine macrophage cells.</p> <p>Methods</p> <p>The investigation was focused on whether OY and fermented OYs could inhibit the production of pro-inflammatory mediators such as nitric oxide (NO) and prostaglandin (PG) E₂as well as the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, tumor necrosis factor (TNF)-α, interleukin (IL)-6, nuclear factor (NF)-κB and mitogen-activated protein kinases (MAPKs) in LPS-stimulated RAW 264.7 cells.</p> <p>Results</p> <p>We found that OY inhibits a little LPS-induced NO, PGE₂, TNF-α and IL-6 productions as well as the expressions of iNOS and COX-2. Interestingly, the fermentation significantly increased its inhibitory effect on the expression of all pro-inflammatory mediators. Furthermore, the fermented OYs exhibited elevated inhibition on the translocation of NF-κB p65 through reduced IκBα degradation as well as the phosphorylations of extracellular signal-regulated kinase (ERK), p38 and c-Jun NH₂-terminal kinase (JNK) MAPKs than untreated control or original OY.</p> <p>Conclusions</p> <p>Finally, the fermentation by <it>Lactobacillus </it>potentiates the anti-inflammatory effect of OY by inhibiting NF-κB and MAPK activity in the macrophage cells.</p

Management of Hypertension in Chronic Kidney Disease

Chronic kidney disease (CKD) is an increasingly prevalent condition globally and is strongly associated with incident cardiovascular disease (CVD). Hypertension is both a cause and effect of CKD and affects the vast majority of CKD patients. Control of hypertension is important in those with CKD as it leads to slowing of disease progression as well as reduced CVD risk. Existing guidelines do not offer a consensus on optimal blood pressure (BP) targets. Therefore, an understanding of the evidence used to create these guidelines is vital when considering how best to manage individual patients. Non-pharmacological interventions are useful in reducing BP in CKD but are rarely sufficient to control BP adequately. Patients with CKD and hypertension will often require a combination of antihypertensive medications to achieve target BP. Certain pharmacological therapies provide additional BP-independent renoprotective and/or cardioprotective action and this must be considered when instituting therapy. Managing hypertension in the context of haemodialysis and following kidney transplantation presents further challenges. Novel therapies may enhance treatment in the near future. Importantly, a personalised and evidence-based management plan remains key to achieving BP targets, reducing CVD risk and slowing progression of CKD.</p

Strategies to improve the bacteriocin protection provided by lactic acid bacteria

peer-reviewedLactic acid bacteria (LAB) produce a wide variety of antimicrobial peptides (bacteriocins) which contribute to the safety and preservation of fermented foods. This review discusses strategies that have been or could be employed to further enhance the commercial application of bacteriocins and/or bacteriocin-producing LAB for for food use.Food Institutional Research Measure of the Department of Agriculture, Fisheries and Food (grant no. 04R and DC); Science Foundation of Ireland (grant no. 07/CE/B1368)