Brigid or Brigantia: A 'Pan-Celtic' Goddess in Profile

This poster examines the goddess Brigid through Roman inscriptions and medieval literature from Great Britain and Ireland. Using a mix of etymological, geographical and epigraphical research methods, I show the many faces and adaptations of this originally Roman ‘Pan-Celtic’ goddess across Europe

An improved method for surface immobilisation of RNA: application to small Non-Coding RNA - mRNA pairing

Characterisation of RNA and its intermolecular interactions is increasing in importance as the inventory of known RNA functions continues to expand. RNA-RNA interactions are central to post-transcriptional gene regulation mechanisms in bacteria, and the interactions of bacterial small non-coding RNAs (sRNAs) with their mRNA targets are the subject of much current research. The technology of surface plasmon resonance (SPR) is an attractive approach to studying these interactions since it is highly sensitive, and allows interaction measurements to be recorded in real-time. Whilst a number of approaches exist to label RNAs for surface-immobilisation, the method documented here is simple, quick, efficient, and utilises the high-affinity streptavidin-biotin interaction. Specifically, we ligate a biotinylated nucleotide to the 3' end of RNA using T4 RNA ligase. Although this is a previously recognised approach, we have optimised the method by our discovery that the incorporation of four or more adenine nucleotides at the 3' end of the RNA (a poly-A-tail) is required in order to achieve high ligation efficiencies. We use this method within the context of investigating small non-coding RNA (sRNA)-mRNA interactions through the application of surface technologies, including quantitative SPR assays. We first focus on validating the method using the recently characterised Escherichia coli sRNA-mRNA pair, MicA-ompA, specifically demonstrating that the addition of the poly-A-tail to either RNA does not affect its subsequent binding interactions with partner molecules. We then apply this method to investigate the novel interactions of a Vibrio cholerae Qrr sRNA with partner mRNAs, hapR and vca0939; RNA-RNA pairings that are important in mediating pathogenic virulence. The calculated binding parameters allow insights to be drawn regarding sRNA-mRNA interaction mechanisms

Hfq binding changes the structure of Escherichia coli small noncoding RNAs OxyS and RprA, which are involved in the riboregulation of rpoS

OxyS and RprA are two small noncoding RNAs (sRNAs) that modulate the expression of rpoS, encoding an alternative sigma factor that activates transcription of multiple Escherichia coli stress-response genes. While RprA activates rpoS for translation, OxyS down-regulates the transcript. Crucially, the RNA binding protein Hfq is required for both sRNAs to function, although the specific role played by Hfq remains unclear. We have investigated RprA and OxyS interactions with Hfq using biochemical and biophysical approaches. In particular, we have obtained the molecular envelopes of the Hfq–sRNA complexes using small-angle scattering methods, which reveal key molecular details. These data indicate that Hfq does not substantially change shape upon complex formation, whereas the sRNAs do. We link the impact of Hfq binding, and the sRNA structural changes induced, to transcript stability with respect to RNase E degradation. In light of these findings, we discuss the role of Hfq in the opposing regulatory functions played by RprA and OxyS in rpoS regulation

Dental Attendances to General Medical Practitioners in Wales: a 44 Year Analysis

One-third of the UK population is composed of problem-oriented dental attenders, seeking dental care only when they have acute dental pain or problems. Patients seek urgent dental care from a range of health care professionals, including general medical practitioners. This study aimed to identify trends in dental attendance at Welsh medical practices over a 44-y period, specifically in relation to dental policy change and factors associated with repeat attendance. A retrospective observational study was completed via the nationwide Secure Anonymised Information Linkage (SAIL) Databank of visits to general medical practice in Wales. Read codes associated with dental diagnoses were extracted for patients attending their general medical practitioner between 1974 and 2017. Data were analyzed with descriptive statistics and univariate and multivariable logistic regression. Over the 44-y period, there were 439,361 dental Read codes, accounting for 288,147 patient attendances. The overall attendance rate was 2.60 attendances per 1,000 patient-years (95% CI, 2.59 to 2.61). The attendance rate was negligible through 1987 but increased sharply to 5.0 per 1,000 patient-years in 2006 (95% CI, 4.94 to 5.09) before almost halving to 2.6 per 1,000 in 2017 (95% CI, 2.53 to 2.63) to a pattern that coincided with changes to National Health Service policies. Overall 26,312 patients were repeat attenders and were associated with living in an area classified as urban and deprived (odds ratio [OR], 1.22; 95% CI, 1.19 to 1.25; P < 0.0001) or rural (OR, 0.84; 95% CI, 0.83 to 0.85; P < 0.0001). Repeat attendance was associated with greater odds of having received an antibiotic prescription (OR, 2.53; 95% CI, 2.50 to 2.56; P < 0.0001) but lower odds of having been referred to another service (OR, 0.75; 95% CI, 0.70 to 0.81; P < 0.0001). Welsh patients’ reliance on medical care for dental problems was influenced by social deprivation and health policy. This indicates that future interventions to discourage dental attendance at medical practitioners should be targeted at those in the most deprived urban areas or rural areas. In addition, health policy may influence attendance rates positively and negatively and should be considered in the future when decisions related to policy change are made

Population dynamics of introduced rodents in Hawai'i Volcanoes National Park 1986-1990

Reports were scanned in black and white at a resolution of 600 dots per inch and were converted to text using Adobe Paper Capture Plug-in.We determined seasonal and geographical distribution patterns for four species of introduced rodents in Hawai‘i Volcanoes National Park from 1986-1990. We surveyed black rats (Rattus rattus), Polynesian rats (R. exulans), Norway rats (R. norvegicus) and house mice (Mus musculus) along an elevation gradient ranging from 90–1,820 m above sea level in five different sites using baited snap traps. Rodent community structure differed by elevation: there were more mice at montane sites and more Polynesian rats in the lowlands. We found that breeding occurred throughout the year for all species at all sites but that seasonal peaks in reproductive activity were common. Reproduction tended to be more common in the summer months at higher elevation sites and in the winter months at lower elevations. Rodents of all species were more abundant in our study in the winter than in the summer, but the differences were not significant. The overall sex ratio did not vary from a 1:1 ratio, but seasonally there were differences in sex ratio which varied with species and site. We calculated the minimum distance traveled from an assessment line and found that larger-bodied species traveled longer average distances. Pelage color in black rats was darkest in wet forest which may have adaptive value. Black and Polynesian rats were widespread in almost all habitat types, whereas mice were limited to dry and mesic sites; Norway rats were the rarest component of our sampling and found only in wet montane forest (‘Ōla‘a Forest).This analysis of historical data was made possible in part thanks to support from the National Park Service Inventory and Monitoring Program and the U.S. Geological Survey Invasive Species Program. The authors would like to acknowledge additional support from the Pacific Cooperative Studies Unit, University of Hawai‘i at Mānoa. Any use of trade, product, or firm names in this publication is for descriptive purposes only and does not imply endorsement by the U.S. Government

WHAT ARE THE DIFFERENCES BETWEEN EFFECTIVE AND INEFFECTIVE CHARTER SCHOOLS IN NORTH CAROLINA?

This purpose of this study was to investigate the effectiveness of the North Carolina charter schools. The variables were chosen based on the researcher‘s inference from literature reviews discussing common factors from qualitative charter school studies. The indicators were used to determine if charter schools could be categorized as effective or ineffective were: (1) attendance rates, (2) short suspensions, (3) student teacher ratio, (4) teacher quality, (5) Reading NCE, and (6) Math NCE scores. Charter schools were determined to be effective, if they were ?1? standard deviation point above average for Reading NCE and Math NCE scores. Charter schools were determined to be ineffective, if they were ?1? standard deviation point below average for Reading NCE and Math NCE scores. The data collected for this study was ex-post facto data from the North Carolina Department of Public Instruction‘s (NCDPI) website using the academic school year 2008-09. The data was coded and entered into SPSS creating a quantitative output to be analyzed and interpreted. The quantitative output determined that there were a total of 12 ineffective charter schools and 10 effective charter schools The results indicated that no charter schools in North Carolina were affiliated with a traditional school district. Also, the dependent variables, attendance rate and teacher quality were determined to be statistically significant in determining the effectiveness of a charter school in North Carolina. The other variables were determined to be insignificant in determining the effectiveness of charter schools in North Carolina

A study of receptive vocabulary development and syntax development in mentally retarded children

The development of language has received considerable attention in recent years from speech pathologists, psychologists and educators. Much has been written on the nature and development of the elements and structure of language in normal children, but less attention has been given to the development of vocabulary and syntax in mentally retarded individuals. This study was designed to determine the relationships of language development (vocabulary and syntax) and age (chronological and mental). Twenty-nine mentally retarded children between the ages of three and eight were selected for study. Selection was based on scores from intelligence tests. The Peabody Picture Vocabulary Test (Dunn, 1959) and the Northwestern Syntax Screening Test (Lee, 1969) were administered to these children in order to derive receptive vocabulary, receptive syntax and expressive syntax scores. Observed and derived scores from normative data were recorded. Comparisons within each test were made by statistical analyses in order to determine significant differences between the rate of vocabulary and syntax development in normal and mentally retarded children

Understanding barriers to purchasing healthier, more sustainable food for people living with obesity and food insecurity.

In westernised countries, food insecurity (FI), poorer diet quality, and obesity are disproportionately represented in groups experiencing socio-economic disadvantage. Grocery stores are one promising arena for intervention; however how these settings can facilitate purchasing of healthier, more sustainable food in people living with obesity (PLWO) and FI remains unclear. Using an online survey (N=583), adults residing in England or Scotland with a body mass index of ≥30kg/m2 self-reported on FI, diet quality, and their experiences of shopping in a grocery store for healthy and sustainable food. Using structural equation modelling, greater FI was directly associated with barriers from the food environment (e.g., price), food preparation practices, lower healthy diet knowledge and physical ill-health. Moreover, greater FI was indirectly associated with poorer diet quality via poorer mental health and greater experiences of self-stigma associated with being food insecure. Grocery store interventions based on price or incentivisation were ranked most helpful in supporting healthier, more sustainable purchasing. These findings highlight the challenges faced by this group when shopping and underscore the need for policy development relating to price and affordability at a population-level, and for clinicians to offer tailored, holistic approaches to obesity treatment that acknowledges and minimises stigma and mental health

Use of Engineering Controls and Personal Protective Equipment by Certified Pesticide Applicators

A convenience survey of 702 certified pesticide applicators was conducted in three states to assess the use of 16 types of engineering controls and 13 types of personal protective equipment (PPE). Results showed that 8 out of 16 engineering devices were adopted by more than 50% of the respondents. The type of crop, size of agricultural operation, and the type of pesticide application equipment were found to influence the adoption of engineering controls. Applicators working on large farms, users of boom and hydraulic sprayers, and growers of field crops were more likely to use engineering devices. Respondents reported a high level of PPE use, with chemical-resistant gloves showing the highest level of compliance. An increase in pesticide applicators wearing appropriate headgear was reported. The majority of respondents did not wear less PPE simply because they used engineering controls. Those who did modify their PPE choices when employing engineering controls used tractors with enclosed cabs and/or were vegetable growers

Chronic idiopathic urinary retention:Comorbidity and outcome in 102 individuals

OBJECTIVES: Chronic Idiopathic urinary retention is poorly understood. One small study suggests higher than expected rates of functional neurological disorder and pain comorbidity which may have implications for understanding the disorder. We investigated the frequency of functional neurological disorder, chronic pain other medical and psychiatric comorbidity, triggers of urinary retention, results of urodynamic assessment, medication history, management, and outcome in patients with chronic idiopathic urinary retention.METHODS: A consecutive retrospective electronic notes analysis was undertaken of patients with chronic idiopathic urinary retention presenting to a secondary care urology clinic between Jan 2018-Jan 2021, with follow-up to their most recent urological appointment.RESULTS: 102 patients were identified (mean age of 41.9 years, 98% female). 25% had functional neurological disorder (n = 26), most commonly limb weakness (n = 19, 19%) and functional seizures (n = 16, 16%). Chronic pain (n = 58, 57%) was a common comorbidity. Surgical and medical riggers to urinary retention were found in almost half of patients (n = 49, 48%). 81% of patients underwent urodynamic assessment (n = 83). Most frequently no specific abnormality was reported (n = 30, 29%). Hypertonic urethral sphincter was the most identified urodynamic abnormality (n = 17, 17%). We noted high levels of opioid (n = 50, 49%) and benzodiazepine (n = 27, 26%) use. Urinary retention resolved in only a small number of patients (n = 6, 6%, median follow up 54 months), in three cases spontaneously.CONCLUSION: This preliminary data suggests idiopathic urinary retention is commonly comorbid with functional neurological disorder, and chronic pain, suggesting shared mechanisms.</p