Bystander effectors of chondrosarcoma cells irradiated at different LET impair proliferation of chondrocytes

While the dose-response relationship of radiation-induced bystander effect (RIBE) is controversial at low and high linear energy transfer (LET), mechanisms and effectors of cell-to-cell communication stay unclear and highly dependent of cell type. In the present study, we investigated the capacity of chondrocytes in responding to bystander factors released by chondrosarcoma cells irradiated at different doses (0.05 to 8 Gy) with X-rays and C-ions. Following a medium transfer protocol, cell survival, proliferation and DNA damages were quantified in bystander chondrocytes. The bystander factors secreted by chondrosarcoma cells were characterized. A significant and major RIBE response was observed in chondrocyte cells (T/C-28a2) receiving conditioned medium from chondrosarcoma cells (SW1353) irradiated with 0.1 Gy of X-rays and 0.05 Gy of C-ions, resulting in cell survivals of 36% and 62%, respectively. Micronuclei induction in bystander cells was observed from the same low doses. The cell survival results obtained by clonogenic assays were confirmed using impedancemetry. The bystander activity was vanished after a heat treatment or a dilution of the conditioned media. The cytokines which are well known as bystander factors, TNF-alpha and IL-6, were increased as a function of doses and LET according to an ELISA multiplex analysis. Together, the results demonstrate that irradiated chondrosarcoma cells can communicate stress factors to non-irradiated chondrocytes, inducing a wide and specific bystander response related to both doses and LET

Study of bystander signals emitted by cartilage 3D culture cells after irradiation in vitro with X-rays and Carbon ions

Lors d’une radiothérapie conventionnelle (Rayons X) ou non-conventionnelle (Hadronthérapie), l’impact des irradiations sur les tissus sains pose des questions essentielles de radiobiologie, ces tissus sains se trouvant sur le trajet du faisceau lors du traitement d’une tumeur. Parmi ces questions, quel est l’impact de l’effet Bystander radio-induit ? Ce mécanisme fait intervenir des signaux de stress encore mal identifiés, émis par une ou plusieurs cellules irradiées vers les cellules non irradiées adjacentes ou très proches, pouvant produire des effets biologiques proches de ceux obtenus dans la zone irradiée.Afin d’étudier ce phénomène, nous avons utilisé différentes techniques de biologie moléculaire dont : des tests de clonogénicité permettant d’étudier la survie des cellules après un traitement, des électrophorèses bidimensionnelles permettant l’analyse du protéome cellulaire, des analyses de milieux conditionnés permettant d’identifier les signaux Bystander émis par les cellules irradiées et des expériences de protéo-génomique visant à étudier des cellules en mélange. En parallèle de ces techniques d’étude, nous avons développé un modèle de culture de cellules en 3D via l’utilisation de « pellets » (agrégats cellulaires).Nos résultats ont montré une diminution de la survie des cellules bystander après transfert de milieu provenant de cellules irradiées, l'implication potentiel de certaines cytokines dans la signalisation bystander ainsi que plusieurs protéines candidates pouvant expliquer en partie la réponse bystander.In conventional radiotherapy (X-ray) or unconventional radiotherapy (Hadrontherapy), the impact of irradiation on healthy tissue raises essential questions of radiobiology, these healthy tissues being in the path of the beam during the treatment of a patient. tumor. Among these questions, what is the impact of the radio-induced Bystander effect? This mechanism involves stress signals still poorly identified, emitted by one or more irradiated cells to adjacent or very close non-irradiated cells, which can produce biological effects close to those obtained in the irradiated zone.To study this phenomenon, we used various molecular biology techniques including: clonogenic assay to study the survival fraction of cells after treatment, two-dimensional electrophoresis allowing the analysis of the cellular proteome, conditioned medium analysis for identify Bystander signals emitted by irradiated cells and proteomembrane experiments aimed at studying cells in a mix. In parallel with these study techniques, we have developed a 3D cell culture model via the use of "pellets" (cell aggregates).Our results showed a decrease in the survival of bystander cells after transfer of medium from irradiated cells, the potential involvement of some cytokines in bystander signaling as well as several candidate proteins that may partly explain the bystander response

Etude des signaux bystander émis par des cellules de cartilage cultivées en 3D et irradiées in vitro dans un contexte de radiothérapie conventionnelle et d'Hadronthérapie

In conventional radiotherapy (X-ray) or unconventional radiotherapy (Hadrontherapy), the impact of irradiation on healthy tissue raises essential questions of radiobiology, these healthy tissues being in the path of the beam during the treatment of a patient. tumor. Among these questions, what is the impact of the radio-induced Bystander effect? This mechanism involves stress signals still poorly identified, emitted by one or more irradiated cells to adjacent or very close non-irradiated cells, which can produce biological effects close to those obtained in the irradiated zone.To study this phenomenon, we used various molecular biology techniques including: clonogenic assay to study the survival fraction of cells after treatment, two-dimensional electrophoresis allowing the analysis of the cellular proteome, conditioned medium analysis for identify Bystander signals emitted by irradiated cells and proteomembrane experiments aimed at studying cells in a mix. In parallel with these study techniques, we have developed a 3D cell culture model via the use of "pellets" (cell aggregates).Our results showed a decrease in the survival of bystander cells after transfer of medium from irradiated cells, the potential involvement of some cytokines in bystander signaling as well as several candidate proteins that may partly explain the bystander response.Lors d’une radiothérapie conventionnelle (Rayons X) ou non-conventionnelle (Hadronthérapie), l’impact des irradiations sur les tissus sains pose des questions essentielles de radiobiologie, ces tissus sains se trouvant sur le trajet du faisceau lors du traitement d’une tumeur. Parmi ces questions, quel est l’impact de l’effet Bystander radio-induit ? Ce mécanisme fait intervenir des signaux de stress encore mal identifiés, émis par une ou plusieurs cellules irradiées vers les cellules non irradiées adjacentes ou très proches, pouvant produire des effets biologiques proches de ceux obtenus dans la zone irradiée.Afin d’étudier ce phénomène, nous avons utilisé différentes techniques de biologie moléculaire dont : des tests de clonogénicité permettant d’étudier la survie des cellules après un traitement, des électrophorèses bidimensionnelles permettant l’analyse du protéome cellulaire, des analyses de milieux conditionnés permettant d’identifier les signaux Bystander émis par les cellules irradiées et des expériences de protéo-génomique visant à étudier des cellules en mélange. En parallèle de ces techniques d’étude, nous avons développé un modèle de culture de cellules en 3D via l’utilisation de « pellets » (agrégats cellulaires).Nos résultats ont montré une diminution de la survie des cellules bystander après transfert de milieu provenant de cellules irradiées, l'implication potentiel de certaines cytokines dans la signalisation bystander ainsi que plusieurs protéines candidates pouvant expliquer en partie la réponse bystander

Study of bystander signals emitted by chondrosarcoma cells irradiated in vitro with X-rays and carbon ions

International audienceCancer is the fisrt cause of death in France. It’s necessary to understand the resistant mechanism of tumors to current therapy (radiotherapy and chemotherapy). Hadrontherapy by Carbon irradiation seems to be a good alternative. The hadrontherapy uses accelerated ions. It’s a technique particularly interesting when the tumor is isolated and located in vital organs where it’s essential to minimize the irradiation dose in healthy arrounding tissue. The ion beams (proton or carbon) have a specific advantage over photons. When they enter the tissues, ions deposit a large part of their energy at a given depth, at the "Bragg peak, beyond, the dose decreases abruptly, which completely protects the healthy structures behind the tumor. At the same dose, protons produce the same biological effects as photons but the ballistic precision of proton allows to increase the dose to treat the tumor without touching healthy tissue. Chondrosarcoma is a good candidate for Hadrontherapy because this cancer is radio and chemo-resistant tumor. However , it’s necessary to evaluate the secondary effect of this type of irradiation especially the interaction between irradiated and non irradiated cells, there is a biological imprecision due to Bystander effect

Proteomic analysis of the Radiation-Induced Bystander Effects

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Study of bystander signals emitted by chondrosarcoma cells irradiated in vitro with X-rays and carbon ions

International audienceCancer is the fisrt cause of death in France. It’s necessary to understand the resistant mechanism of tumors to current therapy (radiotherapy and chemotherapy). Hadrontherapy by Carbon irradiation seems to be a good alternative. The hadrontherapy uses accelerated ions. It’s a technique particularly interesting when the tumor is isolated and located in vital organs where it’s essential to minimize the irradiation dose in healthy arrounding tissue. The ion beams (proton or carbon) have a specific advantage over photons. When they enter the tissues, ions deposit a large part of their energy at a given depth, at the "Bragg peak, beyond, the dose decreases abruptly, which completely protects the healthy structures behind the tumor. At the same dose, protons produce the same biological effects as photons but the ballistic precision of proton allows to increase the dose to treat the tumor without touching healthy tissue. Chondrosarcoma is a good candidate for Hadrontherapy because this cancer is radio and chemo-resistant tumor. However , it’s necessary to evaluate the secondary effect of this type of irradiation especially the interaction between irradiated and non irradiated cells, there is a biological imprecision due to Bystander effect

P23 - Bystander effectors of chondrosarcoma cells irradiated at different LET impair proliferation of chondrocytes

International audienceX-rays / Carbon Ion Tumor (Chondrosarcoma) Non irradiated area arrounding tumor Non irradiated area arrounding healthy tissue Direct effect of irradiation Bystander effect Bystander effectors of chondrosarcoma cells irradiated at different LET impair proliferation of chondrocyte

Discriminating sub-population responses of a mixture of human cell lines by proteogenomics

International audienceMonitoring proteome dynamics from different human cell types present concomitantly in a given sample is of great interest and could be applied to ultra-precise molecular characterization of complex tissues. Here, we propose a proteogenomics-based strategy to point at cell line molecular signatures. For this, the proteome is analyzed by high-throughput shotgun mass spectrometry and specific bioinformatics search are performed. First, mRNA from chondrosarcoma cells (SW1353 cell line) and immortalized chondrocytes (T/C28A2 cell line) were sequenced by RNAseq for establishing the most appropriate protein sequence database. For this an innovative cascade search allows to conciliate de novo and mapping RNAseq assemblies and the Human swissprot databases (Cogne et al., 2018). A set of 2 million of discriminating peptide sequences of the two cell lines are then identified. From them, 480 peptide sequences were detected and monitored based on extracted ion chromatogram (XIC) signals recorded by tandem mass spectrometry. A list of 55 peptides were used for quantitating the ratio of each cell type in a given co-culture sample with high precision selected with cell lines mixed at 2:1, 1:1; and 1:2 ratio. This new methodology was used to analyze the bystander effect generated by irradiated chondrosarcoma cells (SW1353 cell line) on immortalized chondrocytes (T/C28A2 cell line) in co-culture conditions. Such strategy could be applied to investigate intercellular interactions between different cell types, paving the way to new insights into the molecular mechanisms of crosstalk between human cells

Bystander effectors of chondrosarcoma cells irradiated at different LET impair proliferation of chondrocytes

International audienceX-rays / Carbon Ion Tumor (Chondrosarcoma) Non irradiated area arrounding tumor Non irradiated area arrounding healthy tissue Direct effect of irradiation Bystander effect Bystander effectors of chondrosarcoma cells irradiated at different LET impair proliferation of chondrocyte

P47. Proteomic analysis of the Radiation-Induced Bystander Effects between low-dose irradiated Chondrosarcoma cells and Bystander Chondrocytes

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