1,367 research outputs found
Analytical and clinical evaluation of an electrochemiluminescence immunoassay for the determination of CA 125
The CA 125 II assay on the Elecsys(R) 2010 analyzer was evaluated in an
international multicenter trial. Imprecision studies yielded within-run
CVs of 0.8-3.3% and between-day CVs of 2.4-10.9%; CVs for total
imprecision in the manufacturer's laboratory were 2.4-7.8%. The linear
range of the assay extended to at least 4500 kilounits/L (three decades).
Interference from triglycerides (10.3 mmol/L), bilirubin (850 micromol/L),
hemoglobin (1.1 mmol/L), anticoagulants (plasma), and several widely used
drugs was undetectable. Method comparisons with five other CA 125 II
assays showed good correlation but differences in standardization. A 95th
percentile cutoff value of 35 kilounits/L was calculated from values
measured in 593 apparently healthy (pre- and postmenopausal) women. In 95%
of patients with benign gynecological diseases CA 125 was </=190
kilounits/L; 63% of patients with newly diagnosed ovarian carcinoma had
values >190 kilounits/L. A comparison of CA 125 values obtained with the
Elecsys test and with other common CA 125 tests in monitored patients
being treated for ovarian cancer showed identical patterns. In conclusion,
the Elecsys CA 125 II assay is linear over a broad range, yields precise
and accurate results, is free from interferences, and compares well with
other assays
Improved approach for chondrogenic differentiation of human induced pluripotent stem cells
Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for hyaline cartilage regeneration. However, current approaches for chondrogenic differentiation of hiPSCs are complicated and inefficient primarily due to intermediate embryoid body formation, which is required to generate endodermal, ectodermal, and mesodermal cell lineages. We report a new, straightforward and highly efficient approach for chondrogenic differentiation of hiPSCs, which avoids embryoid body formation. We differentiated hiPSCs directly into mesenchymal stem /stromal cells (MSC) and chondrocytes. hiPSC-MSC-derived chondrocytes showed significantly increased Col2A1, GAG, and SOX9 gene expression compared to hiPSC-MSCs. Following transplantation of hiPSC-MSC and hiPSC-MSC-derived chondrocytes into osteochondral defects of arthritic joints of athymic rats, magnetic resonance imaging studies showed gradual engraftment, and histological correlations demonstrated hyaline cartilage matrix production. Results present an efficient and clinically translatable approach for cartilage tissue regeneration via patient-derived hiPSCs, which could improve cartilage regeneration outcomes in arthritic joints
Z boson pair production at LHC in a stabilized Randall-Sundrum scenario
We study the Z boson pair production at LHC in the Randall-Sundrum scenario
with the Goldberger-Wise stabilization mechanism. It is shown that
comprehensive account of the Kaluza-Klein graviton and radion effects is
crucial to probe the model: The KK graviton effects enhance the cross section
of on the whole so that the resonance peak of the radion becomes
easy to detect, whereas the RS effects on the process are
rather insignificant. The and invariant-mass distributions are presented
to study the dependence of the RS model parameters. The production of
longitudinally polarized Z bosons, to which the SM contributions are
suppressed, is mainly due to KK gravitons and the radion, providing one of the
most robust methods to signal the RS effects. The sensitivity bounds
on with are also obtained such that
the effective weak scale of order 5 TeV can be experimentally
probed.Comment: 28 pages, LaTex file, 18 eps figure
The Van der Waals interaction of the hydrogen molecule - an exact local energy density functional
We verify that the van der Waals interaction and hence all dispersion
interactions for the hydrogen molecule given by: W"= -{A/R^6}-{B/R^8}-{C/R^10}-
..., in which R is the internuclear separation, are exactly soluble. The
constants A=6.4990267..., B=124.3990835 ... and C=1135.2140398... (in Hartree
units) first obtained approximately by Pauling and Beach (PB) [1] using a
linear variational method, can be shown to be obtainable to any desired
accuracy via our exact solution. In addition we shall show that a local energy
density functional can be obtained, whose variational solution rederives the
exact solution for this problem. This demonstrates explicitly that a static
local density functional theory exists for this system. We conclude with
remarks about generalising the method to other hydrogenic systems and also to
helium.Comment: 11 pages, 13 figures and 28 reference
Type IIB Solutions with Interpolating Supersymmetries
We study type IIB supergravity solutions with four supersymmetries that
interpolate between two types widely considered in the literature: the dual of
Becker and Becker's compactifications of M-theory to 3 dimensions and the dual
of Strominger's torsion compactifications of heterotic theory to 4 dimensions.
We find that for all intermediate solutions the internal manifold is not
Calabi-Yau, but has SU(3) holonomy in a connection with a torsion given by the
3-form flux. All 3-form and 5-form fluxes, as well as the dilaton, depend on
one function appearing in the supersymmetry spinor, which satisfies a nonlinear
differential equation. We check that the fields corresponding to a flat bound
state of D3/D5-branes lie in our class of solutions. The relations among
supergravity fields that we derive should be useful in studying new gravity
duals of gauge theories, as well as possibly compactifications.Comment: 27pp, v2 REVTeX4, typographical fixes and minor clarifications, v3
added ref, modified discussion of RR axion slightl
Determining the Drift Time of Charge Carriers in P-Type Point-Contact HPGe Detectors
An algorithm to measure the drift time of charge carriers in p-type point
contact (PPC) high-purity germanium (HPGe) detectors from the signals processed
with a charge-sensitive preamplifier is introduced. It is demonstrated that the
drift times can be used to estimate the distance of charge depositions from the
point contact and to characterize losses due to charge trapping. A correction
for charge trapping effects over a wide range of energies is implemented using
the measured drift times and is shown to improve the energy resolution by up to
30%.Comment: 16 pages, 8 figures, submitted to Nucl. Instrum. Meth.
Activating mutations of STAT5B and STAT3 in lymphomas derived from γδ-T or NK cells
Lymphomas arising from NK or γδ-T cells are very aggressive diseases and little is known regarding their pathogenesis. Here we report frequent activating mutations of STAT3 and STAT5B in NK/T-cell lymphomas (n=51), γδ-T-cell lymphomas (n=43) and their cell lines (n=9) through next generation and/or Sanger sequencing. STAT5B N642H is particularly frequent in all forms of γδ-T-cell lymphomas. STAT3 and STAT5B mutations are associated with increased phosphorylated protein and a growth advantage to transduced cell lines or normal NK cells. Growth-promoting activity of the mutants can be partially inhibited by a JAK1/2 inhibitor. Molecular modelling and surface plasmon resonance measurements of the N642H mutant indicate a marked increase in binding affinity of the phosphotyrosine-Y699 with the mutant histidine. This is associated with the prolonged persistence of the mutant phosphoSTAT5B and marked increase of binding to target sites. Our findings suggest that JAK-STAT pathway inhibition may represent a therapeutic strategy. © 2015 Macmillan Publishers Limited. All rights reserved
Astroparticle Physics with a Customized Low-Background Broad Energy Germanium Detector
The MAJORANA Collaboration is building the MAJORANA DEMONSTRATOR, a 60 kg
array of high purity germanium detectors housed in an ultra-low background
shield at the Sanford Underground Laboratory in Lead, SD. The MAJORANA
DEMONSTRATOR will search for neutrinoless double-beta decay of 76Ge while
demonstrating the feasibility of a tonne-scale experiment. It may also carry
out a dark matter search in the 1-10 GeV/c^2 mass range. We have found that
customized Broad Energy Germanium (BEGe) detectors produced by Canberra have
several desirable features for a neutrinoless double-beta decay experiment,
including low electronic noise, excellent pulse shape analysis capabilities,
and simple fabrication. We have deployed a customized BEGe, the MAJORANA
Low-Background BEGe at Kimballton (MALBEK), in a low-background cryostat and
shield at the Kimballton Underground Research Facility in Virginia. This paper
will focus on the detector characteristics and measurements that can be
performed with such a radiation detector in a low-background environment.Comment: Submitted to NIMA Proceedings, SORMA XII. 9 pages, 4 figure
Newtonian and Post Newtonian Expansionfree Fluid Evolution in f(R) Gravity
We consider a collapsing sphere and discuss its evolution under the vanishing
expansion scalar in the framework of gravity. The fluid is assumed to be
locally anisotropic which evolves adiabatically. To study the dynamics of the
collapsing fluid, Newtonian and post Newtonian regimes are taken into account.
The field equations are investigated for a well-known model of the form
admitting Schwarzschild solution. The perturbation scheme is
used on the dynamical equations to explore the instability conditions of
expansionfree fluid evolution. We conclude that instability conditions depend
upon pressure anisotropy, energy density and some constraints arising from this
theory.Comment: 20 pages, accepted for publication in Astrophys. Space Sc
Dalitz plot analysis of D_s+ and D+ decay to pi+pi-pi+ using the K-matrix formalism
FOCUS results from Dalitz plot analysis of D_s+ and D+ to pi+pi-pi+ are
presented. The K-matrix formalism is applied to charm decays for the first time
to fully exploit the already existing knowledge coming from the light-meson
spectroscopy experiments. In particular all the measured dynamics of the S-wave
pipi scattering, characterized by broad/overlapping resonances and large
non-resonant background, can be properly included. This paper studies the
extent to which the K-matrix approach is able to reproduce the observed Dalitz
plot and thus help us to understand the underlying dynamics. The results are
discussed, along with their possible implications on the controversial nature
of the sigma meson.Comment: To be submitted to Phys.Lett.B A misprint corrected in formula
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