56 research outputs found
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Probing helical transitions in a DNA duplexâ€
The complex conformational change from B-DNA to Z-DNA requires
inversion of helix-handedness. Multiple degrees
of freedom are intricately coupled during this transition, and formulating an appropriate reaction coordinate that captures the underlying complexity
would be problematic.
In this contribution, we adopt an alternative approach, based on the potential energy landscape perspective,
to construct a kinetic transition network. Microscopic insight into the transition is provided in terms of geometrically defined discrete paths
consisting of local minima and the transition states that connect them.
We find that the inversion of handedness can occur via two competing mechanisms,
either involving stretched intermediates, or a B-Z junction, in agreement with previous predictions.
The organisation of the free energy landscape further suggests that this process is likely to be slow under physiological conditions.
Our results represent a key step towards decoding the more intriguing features of the transition,
such as the role of ionic strength and negative supercoiling in reshaping the landscape
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A multifunnel energy landscape encodes the competing α-helix and β-hairpin conformations for a designed peptide.
Depending on the amino acid sequence, as well as the local environment, some peptides have the capability to fold into multiple secondary structures. Conformational switching between such structures is a key element of protein folding and aggregation. Specifically, understanding the molecular mechanism underlying the transition from an α-helix to a β-hairpin is critical because it is thought to be a harbinger of amyloid assembly. In this study, we explore the energy landscape for an 18-residue peptide (DP5), designed by Araki and Tamura to exhibit equal propensities for the α-helical and β-hairpin forms. We find that the degeneracy is encoded in the multifunnel nature of the underlying free energy landscape. In agreement with experiment, we also observe that mutation of tyrosine at position 12 to serine shifts the equilibrium in favor of the α-helix conformation, by altering the landscape topography. The transition from the α-helix to the β-hairpin is a complex stepwise process, and occurs via collapsed coil-like intermediates. Our findings suggest that even a single mutation can tune the emergent features of the landscape, providing an efficient route to protein design. Interestingly, the transition pathways for the conformational switch seem to be minimally perturbed upon mutation, suggesting that there could be universal microscopic features that are conserved among different switch-competent protein sequences.erc
epsrc
Cambridge Commonwealth, European, and International Trust
Tighter dimensioning of heterogeneous multi-resource autonomous CPS with control performance guarantees
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Energy landscapes, folding mechanisms, and kinetics of RNA tetraloop hairpins.
RNA hairpins play a pivotal role in a diverse range of cellular functions, and are integral components of ribozymes, mRNA, and riboswitches. However, the mechanistic and kinetic details of RNA hairpin folding, which are key determinants of most of its biological functions, are poorly understood. In this work, we use the discrete path sampling (DPS) approach to explore the energy landscapes of two RNA tetraloop hairpins, and provide insights into their folding mechanisms and kinetics in atomistic detail. Our results show that the potential energy landscapes have a distinct funnel-like bias toward the folded hairpin state, consistent with efficient structure-seeking properties. Mechanistic and kinetic information is analyzed in terms of kinetic transition networks. We find microsecond folding times, consistent with temperature jump experiments, for hairpin folding initiated from relatively compact unfolded states. This process is essentially driven by an initial collapse, followed by rapid zippering of the helix stem in the final phase. Much lower folding rates are predicted when the folding is initiated from extended chains, which undergo longer excursions on the energy landscape before nucleation events can occur. Our work therefore explains recent experiments and coarse-grained simulations, where the folding kinetics exhibit precisely this dependency on the initial conditions.We are grateful to Dr. David de Sancho, Dr. Yassmine Chebaro,
Dr. Guillem Portella, Dr. Chris Whittleston, and Dr. Joanne M.
Carr for helpful discussions. We also thank Mr. Boris Fackovec
for his comments on an initial version of the manuscript. The
work was financially supported by the ERC. D.C. gratefully
acknowledges the Cambridge Commonwealth, European and
International Trust for financial support.This is the accepted manuscript for a paper published in Journal of the American Chemical Society, 2014, 136 (52), pp 18052–18061 DOI: 10.1021/ja510075
Coarse-grained simulations of DNA overstretching
We use a recently developed coarse-grained model to simulate the
overstretching of duplex DNA. Overstretching at 23C occurs at 74 pN in the
model, about 6-7 pN higher than the experimental value at equivalent salt
conditions. Furthermore, the model reproduces the temperature dependence of the
overstretching force well. The mechanism of overstretching is always
force-induced melting by unpeeling from the free ends. That we never see S-DNA
(overstretched duplex DNA), even though there is clear experimental evidence
for this mode of overstretching under certain conditions, suggests that S-DNA
is not simply an unstacked but hydrogen-bonded duplex, but instead probably has
a more exotic structure.Comment: 11 pages, 11 figure
Intrinsically disordered energy landscapes.
Analysis of an intrinsically disordered protein (IDP) reveals an underlying multifunnel structure for the energy landscape. We suggest that such 'intrinsically disordered' landscapes, with a number of very different competing low-energy structures, are likely to characterise IDPs, and provide a useful way to address their properties. In particular, IDPs are present in many cellular protein interaction networks, and several questions arise regarding how they bind to partners. Are conformations resembling the bound structure selected for binding, or does further folding occur on binding the partner in a induced-fit fashion? We focus on the p53 upregulated modulator of apoptosis (PUMA) protein, which adopts an α-helical conformation when bound to its partner, and is involved in the activation of apoptosis. Recent experimental evidence shows that folding is not necessary for binding, and supports an induced-fit mechanism. Using a variety of computational approaches we deduce the molecular mechanism behind the instability of the PUMA peptide as a helix in isolation. We find significant barriers between partially folded states and the helix. Our results show that the favoured conformations are molten-globule like, stabilised by charged and hydrophobic contacts, with structures resembling the bound state relatively unpopulated in equilibrium.The authors thank Prof. Jane Clarke, Dr. Chris Whittleston, Dr. Joanne Carr, Dr. Iskra Staneva and Dr. David de Sancho for helpful discussions. Y.C. and A.J.B. acknowledge funding from the EPSRC grant number EP/I001352/1, D.C. gratefully acknowledges the Cambridge Commonwealth European and International Trust for financial support and D.J.W. the ERC for an Advanced Grant.This is the final version. It was first published by NPG at http://www.nature.com/srep/2015/150522/srep10386/full/srep10386.html?WT.ec_id=SREP-639%2C638-20150526#abstract
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