Development and Characterization of Microsatellite Markers for the Cape Gooseberry Physalis peruviana

Physalis peruviana, commonly known as Cape gooseberry, is an Andean Solanaceae fruit with high nutritional value and interesting medicinal properties. In the present study we report the development and characterization of microsatellite loci from a P. peruviana commercial Colombian genotype. We identified 932 imperfect and 201 perfect Simple Sequence Repeats (SSR) loci in untranslated regions (UTRs) and 304 imperfect and 83 perfect SSR loci in coding regions from the assembled Physalis peruviana leaf transcriptome. The UTR SSR loci were used for the development of 162 primers for amplification. The efficiency of these primers was tested via PCR in a panel of seven P. peruviana accessions including Colombia, Kenya and Ecuador ecotypes and one closely related species Physalis floridana. We obtained an amplification rate of 83% and a polymorphic rate of 22%. Here we report the first P. peruviana specific microsatellite set, a valuable tool for a wide variety of applications, including functional diversity, conservation and improvement of the species

Consistent effects of independent domestication events on the plant microbiota

The effect of plant domestication on plant-microbe interactions remains difficult to prove. In this study, we provide evidence of a domestication effect on the composition and abundance of the plant microbiota. We focused on the genus Phaseolus, which underwent four independent domestication events within two species (P. vulgaris and P. lunatus), providing multiple replicates of a process spanning thousands of years. We targeted Phaseolus seeds to identify a link between domesticated traits and bacterial community composition as Phaseolus seeds have been subject to large and consistent phenotypic changes during these independent domestication events. The seed bacterial communities of representative plant accessions from subpopulations descended from each domestication event were analyzed under controlled and field conditions. The results showed that independent domestication events led to similar seed bacterial community signatures in independently domesticated plant populations, which could be partially explained by selection for common domesticated plant phenotypes. Our results therefore provide evidence of a consistent effect of plant domestication on seed microbial community composition and abundance and offer avenues for applying knowledge of the impact of plant domestication on the plant microbiota to improve microbial applications in agriculture

Ciencias de la Biología y Agronomía

Este volumen I contiene 17 capítulos arbitrados que se ocupan de estos asuntos en Tópicos Selectos de Ciencias de la Biología y Agronomía, elegidos de entre las contribuciones, reunimos algunos investigadores y estudiantes. Se presenta un Estudio Comparativo de los Recursos Hidrológico-Forestales de la Microcuenca de la Laguna de Epatlan, Pue. (1993 a 2014); la Situación Actual de la Mancha de Asfalto en Maíz (Zea mays L.) en los Municipios de Jiquipilas y Ocozocoautla, Chiapas, México; las poblaciones sobresalientes de maíz de la raza Zapalote Chico, en la Región Istmeña de Oaxaca; Se indica el índice de área foliar de cultivo de Chile Poblano mediante dos métodos en condiciones protegidas; Esquivel, Urzúa y Ramírez exploran el efecto de la biofertilización con Azospirillum en el crecimiento y producción de Jitomate; esbozan su artículo sobre la determinación del nivel de Heterosis en híbridos de Maíz para la Comarca Lagunera; una investigación sobre la estabilización de semilla de Solanum lycopersicum durante el almacenamiento y estimulación de la germinación; acotan sobre el CTAB como una nueva opción para la detección de Huanglongbing en cítricos, plantean su evaluación sobre el aluminio y cómo afecta la vida de florero de Heliconia psittacorum; indican sobre el impacto del H-564C, como un híbrido de maíz con alta calidad de proteina para el trópico húmedo de México; presetan su investigación sobre la producción de Piña Cayena Lisa y MD2 (Ananas comosus L.) en condiciones de Loma Bonita, en Oaxaca; acotan sobre el efecto de coberteras como control biológico por conservación contra áfidos en Nogal Pecanero; esbozan sobre la caracterización de cuatro genotipos de Frijol Negro en Martínez de la Torre, Veracruz, México; presentan una caracterización hidroecológica de la microcuenca de Arroyo Prieto, Yuriría, Gto., y alternativas para su restauración ambiental; presentan su investigación sobre el efecto del hongo Beauveria bassiana sobre solubilización de fosfatos y la disponibilidad de fósforo en el suelo; plantean su investigación sobre la Germinación y regeneración in vitro de Epidendrum falcatum LINDL; esbozan su artículo sobre genotipos de frijol negro y su tolerancia a sequía terminal en Veracruz, México

DARWIN Y LA DOMESTICACIÓN DE PLANTAS EN LAS AMÉRICAS: EL CASO DEL MAÍZ Y EL FRÍJOL

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 </style> <![endif]--> <p class="MsoNormal" style="text-align: justify; line-height: 200%;"><span lang="ES-TRAD">En este artículo se revisan los aportes que diversos autores, contemporáneos y posteriores a Darwin, han realizado al estudio del origen de las plantas domesticadas, en especial al tema del origen geográfico de los cultivos. Posteriormente se revisa cómo la evidencia arqueológica junto con la evidencia genética han ayudado a comprender el origen de tres de los cultivos americanos más antiguos e importantes en épocas precolombinas y en épocas actuales: el maíz, el fríjol común y el fríjol Lima. Estos cultivos presentan patrones contrastantes de domesticación, mientras el maíz es producto de un solo evento de domesticación, el fríjol común y el fríjol Lima son cada uno de ellos resultado de domesticaciones múltiples e independientes. Se discute la relevancia del fenómeno de las domesticaciones múltiples en nuestra comprensión del origen del síndrome de la domesticación y los aportes que la disciplina Evolución de Cultivos, junto con otras disciplinas, puede realizar para un mejor entendimiento de los procesos de adaptación en las poblaciones naturales y para acelerar el mejoramiento y la adopción de nuevos cultivos al servicio de la humanidad. </span></p><p class="MsoNormal" style="text-align: justify; line-height: 200%;"><span lang="ES-TRAD">Palabras claves: arqueología, genética, domesticación, evolución de cultivos, fitogeografía</span></p> <p class="MsoNormal" style="text-align: justify; line-height: 200%;"><span lang="ES-TRAD"> </span></p> <p class="MsoNormal" style="text-align: center; line-height: 200%;" align="center"><strong><span lang="ES-TRAD">ABSTRACT</span></strong></p> <p class="MsoNormal" style="text-align: justify; line-height: 200%;"><span lang="ES-TRAD"> </span></p> <p class="MsoNormal" style="text-align: justify; line-height: 200%;"><span lang="EN-GB">In the present article, the main contributions that Darwin and other scholars, contemporary and posterior to h

PLANEACION DIDACTICA DE LA ASIGNATURA DE DESARROLLO PERSONAL

GUIA DIDACTICA / PLANEACION DIDACTICA (NMS

Polymorphisms in <i>Physalis peruviana</i> SSR loci.

<p>Polymorphisms in <i>Physalis peruviana</i> SSR loci.</p

SSR loci identified in <i>Physalis peruviana</i> leaf Expressed Sequence Tags (ESTs).

<p>The number of SSR loci identified at coding sequences (CDS) and Untranslated Regions (UTRs) by using perfect and imperfect repeat search criteria.</p

Functional annotation of 10 <i>P. peruviana</i> contigs containing polymorphic SSR markers.

<p>Gene ontology (GO) functional Categories: <b>C</b> = Cellular component, <b>F</b> = Molecular function, <b>P</b> = Biological process.</p

Allelic variation in 30 <i>Physalis peruviana</i> SSR loci.

<p>Allelic variation in 30 <i>Physalis peruviana</i> SSR loci.</p

SSR alleles in eight <i>Physalis</i> genotypes and four polymorphic loci.

<p>The polymorphic SSR loci were visualized in 6% polyacrylamide gels, samples 1–8 correspond to the work code shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026719#pone-0026719-t001" target="_blank">Table 1</a>. M = Molecular size marker, 10 bp DNA Ladder (Invitrogen, Carlsbad, CA).</p