Type I cAMP-Dependent Protein Kinase Delays Apoptosis in Human Neutrophils at a Site Upstream of Caspase-3

Current data suggest that apoptosis controls neutrophil numbers in tissues. We analyzed roles for and the sites of action for the cAMP-dependent protein kinases (cAPKs) in apoptosis induced in human neutrophils by in vitro storage, cycloheximide (CHX) exposure, and anti-Fas exposure. Treatment with 8-chlorophenylthio-cAMP (8-CPT-cAMP) prolonged the time required for 50% of the cells to exhibit apoptotic morphology (t 50) from 16.3 to 41.8 h (in vitro culture), from 2.4 to 7.8 h (CHX), and from 4.8 to 6.5 h (anti-Fas). CHX ± 8-CPT-cAMP did not significantly alter resting intracellular calcium levels and H-89, a selective inhibitor of cAPK, had no effect on apoptosis in the absence of the analogue. In contrast, site-selective cAMP analogues that specifically activated the type I cAPK, but not type II cAPK, synergistically attenuated apoptosis. Exposure to 8-CPT-cAMP delayed, in parallel, the activity of caspase-3 (CPP-32β), whereas mitogen-activated protein kinase kinase (MAPKK) inhibitor, PD98059, had no effect on CHX-induced apoptosis ± 8-CPT-cAMP. Together these results indicate that type I cAPK activation is necessary and sufficient to mediate cAMP-induced delay in human neutrophil apoptosis induced by several mechanisms and suggest that one of the major sites of cAPK action is upstream of caspase-3 (CPP-32β) activation

Influence of Substance Use During Pregnancy on Maternal and Fetal Health

The increase in substance use, whether legal or illegal, represents a growing global public health problem, affecting various social groups, including pregnant women. The main objective of this research is to describe the use of drugs during pregnancy and to analyze the harmful effects that these substances can cause both in the mother and in the fetus. To carry out this study, a qualitative research methodology of exploratory documentary type was used. Relevant data was collected and selected by reviewing documents, books, and journals published from 2015 to the present. This exhaustive bibliographical review provided us with the necessary support to develop this research work. The findings showed that the consumption of illicit drugs during pregnancy entails a high-risk situation both for the health of the mother and for the development of the fetus. In addition, it was possible to establish that the onset and duration of the neonatal abstinence syndrome vary depending on the type of drug consumed, the amount ingested, and the time of consumption in relation to the date of delivery. In the conclusions of the study, it has been shown that the use of drugs, both legal and illegal, is more frequent in pregnant women. There are many complications that can arise because of drug use during pregnancy, affecting both the mother and the fetus

Drug Use During Pregnancy: Implications for Maternal and Neonatal Health

Drug use during pregnancy, both legal and illegal, is a growing public health problem that affects pregnant women and has serious repercussions on maternal and neonatal health. This daily consumption of multiple drugs, including socially accepted substances, increases the risk of complications and the development of neonatal abstinence syndrome. It is essential to provide education and specialized care to these women, implementing prevention strategies and a multidisciplinary approach. Lack of knowledge about the management of neonatal abstinence syndrome and breastfeeding in cases of active use are areas of research and action needed to improve maternal and neonatal outcomes. In conclusion, comprehensive intervention and adequate education are required to reduce the risks associated with drug use during pregnanc

Eficacia de las promociones a través de Facebook y las ventas en la Estética Láser Renasce de Los Olivos, 2019

La presente investigación tiene como objetivo principal determinar la relación entre la eficacia de las 12 promociones a través de Facebook y las ventas en la estética Láser Renasce de Los Olivos,2019. Para ello se hizo una investigación de enfoque cuantitativo, de nivel correlacional, de tipo aplicada y de diseño no experimental, transversal. Como instrumento de recolección de datos se utilizó la ficha de registro, el cual fue aplicada en nuestro estudio, cuya población total estuvo conformada por 12 promociones a través de Facebook de la Estética Láser Renasce de Los Olivos. Finalmente, los resultados se concluyeron que sí existe relación significativa entre la eficacia de las 12 promociones a través de Facebook y Las ventas en la Estética Láser Renasce de Los Olivos, 2019, esto nos dice que las interacciones de los usuarios lograron excelentes resultados con respecto a las ventas y además de que los usuarios interactúen y finalmente hagan uso del servicio. Además de que las interacciones por parte de los usuarios o clientes se ven reflejados en las ventas de la Estética Láser Renasce de Los Olivos

Filoviridae: insights into immune responses to Ebola virus

Ebola virus (EBOV) is a zoonotic virus comprising of six known different species, designated within the family Filoviridae and genus Ebolavirus. The first recorded outbreak of an EBOV disease (EVD) was in Yambuku, Zaire EBOV (ZEBOV) in 1976, followed by the Sudan EBOV (SUDV) later that year. Outbreaks have been increasing throughout the 21st century, and mortality rates can reach up to 90%. Such extraordinary virulence is evidenced by a few pathogens, similar to the Marburg virus (MARV) that originated in Uganda and was first detected in Germany in 1967. The virulent nature of filovirus disease has established these related viruses as a formidable global concern. There are currently four types of Ebolaviridae species known to infect humans, with two more recently identified in other animals that are genomically different concerning cellular pathogenesis or aetiology of disease. Recent advances in understanding the pathogenesis of filovirus disease infections have been remarkable, yet the immunological response to filovirus infection remains unknown. Scientific analysis of cellular mechanisms can provide insight into virulence factors utilised by other pathogenic viruses that also cause febrile illness with occasional haemorrhagic fever in humans. In this review, a brief summary of EBOV protein structure and functional cellular effects is covered. The role of innate and adaptive immune cells known since 1976 is considered with the relevance and implications of immunological proteins measured by cluster of differentiation (CD) molecule, alongside cytokine, chemokine, and other biologically relevant pathways, and through genetic research. A thorough understanding of immunological correlates affecting host responses to EBOV will facilitate clinical and applied research knowledge, contributing to protection against potential public health threats

Planeacion estrategica de tecnologias de informacion para el hospital Hernando Quintero Blanco

las graficas y diagramas se encuentran relacionadas en la parte del objetivo numero cuatro del trabajo de grado en la gestion de la propuesta tecnico operativaEste trabajo de grado se enmarca en la planeación estratégica de tecnologías de información aplicada al hospital municipal Hernando Quintero Blanco del municipio de el Paso (Cesar), la cual esta soportada bajo la metodología del Balanced ScoreCard y la aplicación de los lineamientos del PMBOK. Asimismo, en la ejecución de este proyecto se permitió establecer el logro de la planeación estratégica en dos dependencias de la organización objeto de estudio, las cuales fueron el area de recursos humanos y el area de gestión de calidad, demostrando mediante esta óptica de trabajo, que las organizaciones empresariales pueden ser sustentables través de sus mismos recursos tecnológicos, utilizando el apalancamiento de las estrategias predictivas que ayudan al desarrollo de las buenas prácticas de gestión en las tecnologías de información e innovación. En este orden de ideas, la aplicación del Balanced ScoreCard logro el cumplimiento de las metas planteadas en los objetivos trazados en el hospital Hernando Quintero Blanco, tales como: la falta de nuevas dependencias para el departamento de TI+i, falta de seguridad de la información, gestión de la información, entre otros parámetros; demostrando que el área de tecnologías de información e innovación, hacen parte fundamental en los ejes de apoyo estratégico para el cumplimiento de las metas planteadas, controlando y asegurando la información captada mediante el uso del Balanced ScoreCard con los objetivos de control, encontrando las relaciones de causa-efecto entre las perspectivas del negocio, determinando cuáles de los objetivos de TI+i se están cumpliendo o no. Por consiguiente, el despliegue de las dos metodologías propuestas para la implantación del modelo de planeación estratégica de tecnologías de información, permitió concluir el plan estratégico de la organización, e iniciar el proceso del mismo con la determinación del nivel de madurez de gestión de tecnologías de información. Del mismo modo, con la construcción del modelo, se establecieron las acciones que permitieron cerrar la brecha que existe entre los esfuerzos de tecnologías de información e innovación y las estrategias de la organización expresadas tanto en procesos como en dependencias, generando acciones correctivas y preventivas, las cuales fueron ajustadas mediante un amplio marco teórico de regulación en el área de aplicación de procesos, actividades y beneficios de la situación actual de la infraestructura tecnológica del hospital Hernando Quintero Blanco.This degree work is part of the strategic planning of information technologies applied to the Hernando Quintero Blanco municipal hospital in the municipality of El Paso (Cesar), which is supported under the Balanced ScoreCard methodology and the application of the PMBOK guidelines. Likewise, in the execution of this project it was allowed to establish the achievement of strategic planning in two units of the organization under study, which were the area of human resources and the area of quality management, demonstrating through this work perspective, that business organizations can be sustainable through their same technological resources, using the leverage of predictive strategies that help the development of good management practices in information and innovation technologies. In this order of ideas, the application of the Balanced ScoreCard achieved the fulfillment of the goals set in the objectives set in the Hernando Quintero Blanco hospital, such as: the lack of new dependencies for the IT + i department, lack of security of the information, information management, among other parameters; demonstrating that the area of information technologies and innovation, are a fundamental part in the strategic support axes for the fulfillment of the goals set, controlling and ensuring the information captured through the use of the Balanced ScoreCard with the control objectives, finding the relationships of Cause-effect between business perspectives, determining which of the IT + i objectives are being met or not. Therefore, the deployment of the two methodologies proposed for the implementation of the strategic planning model of information technologies, allowed to conclude the strategic plan of the organization, and initiate the process of the same with the determination of the level of maturity of technology management of information. In the same way, with the construction of the model, the actions were established that allowed to close the gap that exists between the efforts of information and innovation technologies and the strategies of the organization expressed both in processes and in dependencies, generating corrective and preventive actions, which were adjusted through a broad theoretical framework of regulation in the area of application of processes, activities and benefits of the current situation of the technological infrastructure of the Hernando Quintero Blanco hospital

Herramienta computacional para la evaluación de calidad de productos software enmarcados en actividades de investigación

El presente artículo describe el diseño de una herramienta computacional para evaluar la calidad de productos software derivados de actividades de investigación, teniendo en cuenta las características propias de este tipo de software. La herramienta gestiona los elementos de un modelo de calidad jerárquico y permite recolectar la información necesaria para evaluar productos intermedios y finales de un desarrollo de software. Utilizando diagramas de paquetes y de casos de uso se muestra la funcionalidad que debe tener, la cual ha sido diseñada a partir de directrices dadas por estándares como el ISO 14598, ISO 9126 e ISO 25000

Proponer la instalación de Paneles Solares de Energía Fotovoltaica en el Jardín Parque Cementerio Los Olivos Ubicado en el Municipio de Cota en el Departamento de Cundinamarca – Colombia.

En los últimos años los proyectos de energía fotovoltaica han tomado mucho auge debido al calentamiento global, y los distintos tratados internacionales que luchan con la conservación del medio ambiente, este auge es en todo el planeta y sur América no es la excepción, los gobiernos están buscando alternativas para suplir y proyectar nuevas formas de obtener energía eléctrica y han mirado en la energía fotovoltaica una solución a media y largo plazo, es por eso que desde los distintos gobiernos vienen ofreciendo exenciones tributarias para proyectos que tengan la finalidad de explorar y crear nuevas formas de energía no renovable. Este proyecto Propone la instalación de paneles solares de Energía Fotovoltaica en el Jardín Parque Cementerio Los Olivos Ubicado en el Municipio de Cota en el Departamento de Cundinamarca (Colombia) este proyecto con el fin de bajar costo de energía de funcionamientos en todos los procesos que se llevan a cabo dentro de la institución.In recent years photovoltaic energy projects have taken a big boom due to global warming, and the various international treaties that struggle with the conservation of the environment, this boom is all over the planet and South America is no exception, governments are looking for alternatives to supply and project new ways to obtain electricity and have looked at photovoltaic energy as a medium and long-term solution, This is why different governments have been offering tax exemptions for projects aimed at exploring and creating new forms of non-renewable energy. This project proposes through a feasibility study the creation of a photovoltaic energy plant in the Los Olivos Cemetery Garden located in the Municipality of Cota in the Department of Cundinamarca (Colombia) this project in order to reduce the energy cost of operations in all the processes that are carried out within the institution