Relação da temperatura mínima diária e temperatura de superfície do sensor MODIS no Brasil.

RESUMO. As geadas podem causar grandes danos às plantações, sendo responsáveis por porcentual significativo das ocorrências de sinistros de natureza meteorológica. Assim, entender sua distribuição espacial e frequência de ocorrência é de grande importância para a gestão dos riscos agrícolas. Temperaturas mínimas registradas em abrigos meteorológicos podem indicar a ocorrência desse fenômento. Já a utilização de produtos de sensoriamento remoto permitem avaliações com melhor resolução espacial em grandes áreas. Assim, avaliou-se a relação entre temperaturas mínimas diárias e os produtos do sensor MODIS para 319 estações meteorológicas no território nacional. Os melhores resultados foram obtidos a partir da temperatura noturna do sensor a bordo do satélite Aqua (MYD11A1) para estados da região Sul, Sudeste e parte do Centro Oeste, principais áreas de ocorrência de geadas. Isso indica que o produto MYD11A1 pode ser utilizado para fins de avaliação de frequência de geadas e monitoramento.Na publicação: Luis Eduardo B. A. Monteiro. Editores: Douglas Francisco Marcolino Gherardi, Ieda Del´Arco Sanches, Luiz Eduardo Oliveira e Cruz de Aragão. SBSR 2019

DNA interaction with Actinomycin D: mechanical measurements reveal the details of the binding data

We have studied the interaction between the anticancer drug Actinomycin D (ActD) and the DNA molecule by performing single molecule stretching experiments and atomic force microscopy (AFM) imaging. From the stretching experiments, we determine how the mechanical properties of the DNA–ActD complexes vary as a function of drug concentration, for a fixed DNA concentration. We have found that the persistence lengths of the complexes formed behave non-monotonically: at low concentrations of ActD they are more flexible than the bare DNA molecule and become stiffer at higher concentrations. On the other hand, the contour length increases monotonically as a function of ActD concentration. Using a two-sites quenched disorder statistical model recently developed by us, we were able to extract chemical parameters such as the intrinsic binding constant and the degree of cooperativity from these pure mechanical measurements, thus performing a robust characterization of the interaction. The AFM images, otherwise, were used to measure the bending angle size distribution that ActD introduces on the double-helix structure and the average number of bendings per DNA molecule as a function of drug concentration, two quantities that cannot be determined from the stretching experiments

Endurance exercise training ameliorates insulin resistance and reticulum stress in adipose and hepatic tissue in obese rats

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Obesity-induced endoplasmatic reticulum (ER) stress has been demonstrated to underlie the induction of obesity-induced JNK and NF-kappa B activation inflammatory responses, and generation of peripheral insulin resistance. On the other hand, exercise has been used as a crucial tool in obese and diabetic patients, and may reduce inflammatory pathway stimulation. However, the ability of exercise training to reverse endoplasmatic reticulum stress in adipose and hepatic tissue in obesity has not been investigated in the literature. Here, we demonstrate that exercise training ameliorates ER stress and insulin resistance in DIO-induced rats. Rats were fed with standard rodent chow (3,948 kcal kg(-1)) or high-fat diet (5,358 kcal kg(-1)) for 2 months. After that rats were submitted to swimming training (1 h per day, 5 days for week with 5% overload of the body weight for 8 weeks). Samples from epididymal fat and liver were obtained and western blot analysis was performed. Our results showed that swimming protocol reduces pro-inflammatory molecules (JNK, I kappa B and NF-kappa B) in adipose and hepatic tissues. In addition, exercise leads to reduction in ER stress, by reducing PERK and eIF2 alpha phosphorylation in these tissues. In parallel, an increase in insulin pathway signaling was observed, as confirmed by increases in IR, IRSs and Akt phosphorylation following exercise training in DIO rats. Thus, results suggest that exercise can reduce ER stress, improving insulin resistance in adipose and hepatic tissue.111920152023Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Endurance exercise training ameliorates insulin resistance and reticulum stress in adipose and hepatic tissue in obese rats

Obesity-induced endoplasmatic reticulum (ER) stress has been demonstrated to underlie the induction of obesity-induced JNK and NF-kappa B activation inflammatory responses, and generation of peripheral insulin resistance. On the other hand, exercise has been used as a crucial tool in obese and diabetic patients, and may reduce inflammatory pathway stimulation. However, the ability of exercise training to reverse endoplasmatic reticulum stress in adipose and hepatic tissue in obesity has not been investigated in the literature. Here, we demonstrate that exercise training ameliorates ER stress and insulin resistance in DIO-induced rats. Rats were fed with standard rodent chow (3,948 kcal kg(-1)) or high-fat diet (5,358 kcal kg(-1)) for 2 months. After that rats were submitted to swimming training (1 h per day, 5 days for week with 5% overload of the body weight for 8 weeks). Samples from epididymal fat and liver were obtained and western blot analysis was performed. Our results showed that swimming protocol reduces pro-inflammatory molecules (JNK, I kappa B and NF-kappa B) in adipose and hepatic tissues. in addition, exercise leads to reduction in ER stress, by reducing PERK and eIF2 alpha phosphorylation in these tissues. in parallel, an increase in insulin pathway signaling was observed, as confirmed by increases in IR, IRSs and Akt phosphorylation following exercise training in DIO rats. Thus, results suggest that exercise can reduce ER stress, improving insulin resistance in adipose and hepatic tissue.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Extremo Catarinense, Lab Fisiol & Bioquim Exercicio, Programa Posgrad Ciencias Saude, BR-88806000 Criciuma, SC, BrazilUniversidade Federal de São Paulo, UNIFESP, Sch Phys Educ, Dept Biosci, Santos, SP, BrazilUniv São Paulo, Sch Phys Educ & Sport Ribeirao Preto, BR-14049 Ribeirao Preto, SP, BrazilUniv Estadual Campinas, UNICAMP, Fac Sci Appl, Limeira, SP, BrazilUniversidade Federal de São Paulo, UNIFESP, Sch Phys Educ, Dept Biosci, Santos, SP, BrazilWeb of Scienc