Elevated Incidence of Dental Caries in a Mouse Model of Cystic Fibrosis

Saliva bicarbonate constitutes the main buffering system which neutralizes the pH fall generated by the plaque bacteria during sugar metabolism. We found that the saliva pH is severely decreased in a mouse model of cystic fibrosis disease (CF). Given the close relationship between pH and caries development, we hypothesized that caries incidence might be elevated in the mouse CF model.). are enhanced at low pH values, we speculate that the decrease in the bicarbonate content and pH buffering of the saliva is at least partially responsible for the increased severity of lesions observed in the CF mouse

Search for photons above 1018 eV by simultaneously measuring the atmospheric depth and the muon content of air showers at the Pierre Auger Observatory

The Pierre Auger Observatory is the most sensitive instrument to detect photons with energies above 1017 eV. It measures extensive air showers generated by ultrahigh energy cosmic rays using a hybrid technique that exploits the combination of a fluorescence detector with a ground array of particle detectors. The signatures of a photon-induced air shower are a larger atmospheric depth of the shower maximum (Xmax) and a steeper lateral distribution function, along with a lower number of muons with respect to the bulk of hadron-induced cascades. In this work, a new analysis technique in the energy interval between 1 and 30 EeV (1 EeV=1018 eV) has been developed by combining the fluorescence detector-based measurement of Xmax with the specific features of the surface detector signal through a parameter related to the air shower muon content, derived from the universality of the air shower development. No evidence of a statistically significant signal due to photon primaries was found using data collected in about 12 years of operation. Thus, upper bounds to the integral photon flux have been set using a detailed calculation of the detector exposure, in combination with a data-driven background estimation. The derived 95% confidence level upper limits are 0.0403, 0.01113, 0.0035, 0.0023, and 0.0021 km-2 sr-1 yr-1 above 1, 2, 3, 5, and 10 EeV, respectively, leading to the most stringent upper limits on the photon flux in the EeV range. Compared with past results, the upper limits were improved by about 40% for the lowest energy threshold and by a factor 3 above 3 EeV, where no candidates were found and the expected background is negligible. The presented limits can be used to probe the assumptions on chemical composition of ultrahigh energy cosmic rays and allow for the constraint of the mass and lifetime phase space of super-heavy dark matter particles

Inference of the Mass Composition of Cosmic Rays with Energies from 1018.5 to 1020 eV Using the Pierre Auger Observatory and Deep Learning

We present measurements of the atmospheric depth of the shower maximum Xmax, inferred for the first time on an event-by-event level using the surface detector of the Pierre Auger Observatory. Using deep learning, we were able to extend measurements of the Xmax distributions up to energies of 100 EeV (1020 eV), not yet revealed by current measurements, providing new insights into the mass composition of cosmic rays at extreme energies. Gaining a 10-fold increase in statistics compared to the fluorescence detector data, we find evidence that the rate of change of the average Xmax with the logarithm of energy features three breaks at 6.5±0.6(stat)±1(syst) EeV, 11±2(stat)±1(syst) EeV, and 31±5(stat)±3(syst) EeV, in the vicinity to the three prominent features (ankle, instep, suppression) of the cosmic-ray flux. The energy evolution of the mean and standard deviation of the measured Xmax distributions indicates that the mass composition becomes increasingly heavier and purer, thus being incompatible with a large fraction of light nuclei between 50 and 100 EeV

Large-scale Cosmic-ray Anisotropies with 19yr of Data from the Pierre Auger Observatory

Results are presented for the measurement of large-scale anisotropies in the arrival directions of ultra–high-energy cosmic rays detected at the Pierre Auger Observatory during 19 yr of operation, prior to AugerPrime, the upgrade of the observatory. The 3D dipole amplitude and direction are reconstructed above 4 EeV in four energy bins. Besides the established dipolar anisotropy in R.A. above 8 EeV, the Fourier amplitude of the 8–16 EeV energy bin is now also above the 5σ discovery level. No time variation of the dipole moment above 8 EeV is found, setting an upper limit to the rate of change of such variations of 0.3% yr−1 at the 95% confidence level. Additionally, the results for the angular power spectrum are shown, demonstrating no other statistically significant multipoles. The results for the equatorial dipole component down to 0.03 EeV are presented, using for the first time a data set obtained with a trigger that has been optimized for lower energies. Finally, model predictions are discussed and compared with observations, based on two source emission scenarios obtained in the combined fit of spectrum and composition above 0.6 EeV

Measurement of the depth of maximum of air-shower profiles with energies between 1018.5 and 1020 eV using the surface detector of the Pierre Auger Observatory and deep learning

We report an investigation of the mass composition of cosmic rays with energies from 3 to 100 EeV (1 EeV=1018 eV) using the distributions of the depth of shower maximum Xmax. The analysis relies on ∼50,000 events recorded by the surface detector of the Pierre Auger Observatory and a deep-learning-based reconstruction algorithm. Above energies of 5 EeV, the dataset offers a 10-fold increase in statistics with respect to fluorescence measurements at the Observatory. After cross-calibration using the fluorescence detector, this enables the first measurement of the evolution of the mean and the standard deviation of the Xmax distributions up to 100 EeV. Our findings are threefold: (i) The evolution of the mean logarithmic mass toward a heavier composition with increasing energy can be confirmed and is extended to 100 EeV. (ii) The evolution of the fluctuations of Xmax toward a heavier and purer composition with increasing energy can be confirmed with high statistics. We report a rather heavy composition and small fluctuations in Xmax at the highest energies. (iii) We find indications for a characteristic structure beyond a constant change in the mean logarithmic mass, featuring three breaks that are observed in proximity to the ankle, instep, and suppression features in the energy spectrum

Association of BMI, lipid-lowering medication, and age with prevalence of type 2 diabetes in adults with heterozygous familial hypercholesterolaemia: a worldwide cross-sectional study

Background: Statins are the cornerstone treatment for patients with heterozygous familial hypercholesterolaemia but research suggests it could increase the risk of type 2 diabetes in the general population. A low prevalence of type 2 diabetes was reported in some familial hypercholesterolaemia cohorts, raising the question of whether these patients are protected against type 2 diabetes. Obesity is a well known risk factor for the development of type 2 diabetes. We aimed to investigate the associations of known key determinants of type 2 diabetes with its prevalence in people with heterozygous familial hypercholesterolaemia. Methods: This worldwide cross-sectional study used individual-level data from the EAS FHSC registry and included adults older than 18 years with a clinical or genetic diagnosis of heterozygous familial hypercholesterolaemia who had data available on age, BMI, and diabetes status. Those with known or suspected homozygous familial hypercholesterolaemia and type 1 diabetes were excluded. The main outcome was prevalence of type 2 diabetes overall and by WHO region, and in relation to obesity (BMI ≥30·0 kg/m2) and lipid-lowering medication as predictors. The study population was divided into 12 risk categories based on age (tertiles), obesity, and receiving statins, and the risk of type 2 diabetes was investigated using logistic regression. Findings: Among 46 683 adults with individual-level data in the FHSC registry, 24 784 with heterozygous familial hypercholesterolaemia were included in the analysis from 44 countries. 19 818 (80%) had a genetically confirmed diagnosis of heterozygous familial hypercholesterolaemia. Type 2 diabetes prevalence in the total population was 5·7% (1415 of 24 784), with 4·1% (817 of 19 818) in the genetically diagnosed cohort. Higher prevalence of type 2 diabetes was observed in the Eastern Mediterranean (58 [29·9%] of 194), South-East Asia and Western Pacific (214 [12·0%] of 1785), and the Americas (166 [8·5%] of 1955) than in Europe (excluding the Netherlands; 527 [8·0%] of 6579). Advancing age, a higher BMI category (obesity and overweight), and use of lipid-lowering medication were associated with a higher risk of type 2 diabetes, independent of sex and LDL cholesterol. Among the 12 risk categories, the probability of developing type 2 diabetes was higher in people in the highest risk category (aged 55–98 years, with obesity, and receiving statins; OR 74·42 [95% CI 47·04–117·73]) than in those in the lowest risk category (aged 18–38 years, without obesity, and not receiving statins). Those who did not have obesity, even if they were in the upper age tertile and receiving statins, had lower risk of type 2 diabetes (OR 24·42 [15·57–38·31]). The corresponding results in the genetically diagnosed cohort were OR 65·04 (40·67–104·02) for those with obesity in the highest risk category and OR 20·07 (12·73–31·65) for those without obesity. Interpretation: Adults with heterozygous familial hypercholesterolaemia in most WHO regions have a higher type 2 diabetes prevalence than in Europe. Obesity markedly increases the risk of diabetes associated with age and use of statins in these patients. Our results suggest that heterozygous familial hypercholesterolaemia does not protect against type 2 diabetes, hence managing obesity is essential to reduce type 2 diabetes in this patient population. Funding: Pfizer, Amgen, MSD, Sanofi-Aventis, Daiichi-Sankyo, and Regeneron

Biologiczna różnorodność fauny motyli na obszarach pogórniczych

In this research we study butterflies diversity and abundance in North Czech Coal Basin (NCCB) and Landscape protected area Ceske stredohori (LP A). Butterflies are very sensitive species for changes in aricultural management (Denis 2004). They can be used as bioindicators and umbrella species. We study 4 localities - each of them includes 3 study plots. Two localities are situated in LPA and two are situated at dumps of (NCCB). We compare three different succesion stages (low xerotherm grassland, mezophilus grassland, areas with trees and bushes) on two different types of landscape (seminatural biotops x reclamations). In 2009 there were done four observations on each locality and two visits were done in 2010. Higher number of species was found in LPA and also more of rare and endangered species were observed there compare to dumps. Altough dumps seem to be good potential habitats for butterflies. Important for all localities is, if there is an active antropogenic management or not.W tym badaniu oceniono różnorodność i ilość motyli na terytorium Zagłębia Węglowego Czech Północnych (North Czech Coal Basin, NCCB) oraz obszaru chronionego Ceske stredohori (LPA). Motyle to rząd (owadów) uskrzydlonych bardzo wrażliwy na zmiany w sposobie zarządzania rolnego (Denis 2004). Gatunki motyli mogą być wykorzystywane jako biologiczne wskaźniki. Badamy cztery lokalizacje, z których każda składa się z trzech działek badawczych. Dwie lokalizacje znajdują się w LPA, a dwie pozostałe na zwałowiskach NCCB. Porównujemy trzy różne etapy sukcesji (niskie murawy kserotermiczne, murawy mezofilne, obszary z drzewami i krzewami) w ramach dwóch, odmiennych typów krajobrazu (seminaturalne biotopy x rekultywacja). W 2009 roku dokonano czterech obserwacji w każdej lokalizacji, a w 2010 roku przeprowadzone zostały dwie wizyty. Większa liczba gatunków została zaobserwowana w LPA. Ponadto w porównaniu ze zwałowiskami odkryto tam więcej gatunków rzadkich i zagrożonych. Niemniej zwałowiska wydają się być odpowiednim, potencjalnym siedliskiem dla motyli. Ważne dla wszystkich lokalizacji jest to, czy aktywne zarządzanie antropogeniczne ma miejsce czy nie