Energía e infraestructuras

Material addicional de la ponència presentada al III Congrés Obert i Virtual Castelló 2020. Castelló de la Plana, 2011-201

Antioxidant potential of Himanthalia elongata for protection against ischemia-reperfusion injury in the small bowel

Background. Seaweed has been associated with the prevention and/or treatment of various diseases related to oxidative stress because of its antioxidant activity. We investigated the protective potential of extract of Himanthalia elongata against ischemia-reperfusion (I/R) injury in the intestine of rats. Methods. Seventy-two (72) male Wistar albino rats were randomly assigned into 12 groups as follows: sham, I/R only, I/R plus vehicle at 3 time points, and I/R plus extract at 3 time points. The degree of intestinal injury was determined by oxidative stress using lipid peroxidation, superoxide dismutase, catalase, and glutathione peroxidase after mesenteric ischemia-reperfusion. A histological study was also performed. Results. The algae extract helps to maintain normal enzymatic levels because, for all the studied parameters, groups treated with the extract showed significant differences (P < .05) compared with the I/R groups, and there were no differences compared with the sham group. The histological study showed that damage to the intestinal mucosa was less severe in animals treated with extract of H elongata after up to 24 hours of reperfusion compared with the I/R group. Conclusion. These results suggest that the extract of H elongata can protect intestinal tissue against ischemia-reperfusion injury. (Surgery 2017;162:577-85.)Biotecnologí

UN RECORRIDO POR FLORENCIA A TRAVÉS DE LA PERCEPCIÓN Y LA FOTOGRAFÍA CALLEJERA

This final project revolves the realization of a digital photos serie, starting of my personal point of view, in a esthetic and personal way, about the cultural and daily themes which we can appreciate through photography, in the city of Florence, and how is the process of perceive all the elements which are a mainly part of the cultural image. The objective of this project is the research to transmite the essence of the streets of Florence, and understand, throught all the images, how we can perceive all around us, building a special relationship with the atmosphere and the individual, and how I use to interact with it and which emotions bring me that image.UN RECORRIDO POR FLORENCIA A TRAVÉS DE LA PERCEPCIÓN Y LA FOTOGRAFÍA CALLEJERA El presente trabajo consiste en la realización y análisis de una serie de fotografías digitales partiendo de un punto de vista estético personal, sobre temas culturales y cotidianos que tienen lugar hoy en día en la ciudad de Florencia y que juegan un papel fundamental a la hora de percibir su legado histórico, cultural y turístico. Es decir, tratamos mostrar la esencia de una ciudad renacentista que perdura en el tiempo, a través de la fotografía contemporánea. Centrándonos especialmente en la imagen callejera, sus gentes, los rostros y la figura, pretendemos hacer una reflexión sobre cómo percibimos e interaccionamos con todas estas composiciones visuales en un determinado espacio y qué emociones se desprenden de ellas, construyendo así una identidad visual de la ciudad de Florencia. Proyectamos hacer un recorrido por ella a través de la identidad artística y poética que desprende, identidades que han cautivado al mundo entero.Cejalvo Herráiz, A. (2017). UN RECORRIDO POR FLORENCIA A TRAVÉS DE LA PERCEPCIÓN Y LA FOTOGRAFÍA CALLEJERA. http://hdl.handle.net/10251/110737TFG

Reinterpretación de referentes escultóricos clásicos adaptados al contexto creativo, experimental y conceptual a través del grabado

[EN] In this project a series of works is carried out in which the theme of classical sculpture is combined with the graphic work from a very personal point of view. Through the project the study of the fundamental elements is carried out from which we proceed to the production of a collection of engravings, in which some of the sculptural referents are reinterpreted and adapted to appropriate colors, matrices, papers and textures, in a creative and experimental context within the field of engraving, to observe its interaction with the plastic and material elements. I try to reflect and recreate the beauty of the classical spirit, where the human figure is placed in a mystical context and through which the divine essence manifests itself. On the other hand, the project is developed within a theoretical and conceptual framework of Latin American influences which will give the main sustenance. The TFM is considered in the context of a continuation of the production of work done in the Master of Artistic Production, in the subject of Engraving Processes.[ES] En este proyecto se lleva a cabo una serie de obras en las cuales se aúna el tema de la escultura clásica con la obra gráfica bajo un punto de vista muy personal. A través del proyecto se realiza el estudio de los elementos fundamentales a partir de los que se procede a la producción de una colección de grabados, en la que algunos de los referentes escultóricos son reinterpretados y adaptados a colores, matrices, papeles y texturas adecuados, en un contexto creativo y experimental dentro del campo del grabado, para observar su interacción con los elementos plásticos y matéricos. Se busca reflejar y recrear la belleza del espíritu clásico, donde la figura humana se coloca en un contexto místico y a través del cual la esencia divina se manifiesta. Por otra parte, el proyecto se desarrolla dentro de un marco teórico y conceptual de influencias latinoamericanas el cual le dará sustento. El TFM se plantea en el contexto de una continuación de la producción de obra realizada en el Máster de Producción Artística, en la asignatura de Procesos de Grabado.Cejalvo Herráiz, A. (2019). Reinterpretación de referentes escultóricos clásicos adaptados al contexto creativo, experimental y conceptual a través del grabado. http://hdl.handle.net/10251/132095TFG

Caregiving role and Psychosocial and individual factors: a systematic review

Emotional intelligence (EI) refers to the ability to perceive, express, understand, and manage emotions. Current research indicates that it may protect against the emotional burden experienced in certain professions. This article aims to provide an updated systematic review of existing instruments to assess EI in professionals, focusing on the description of their characteristics as well as their psychometric properties (reliability and validity). A literature search was conducted in Web of Science (WoS). A total of 2761 items met the eligibility criteria, from which a total of 40 different instruments were extracted and analysed. Most were based on three main models (i.e., skill-based, trait-based, and mixed), which differ in the way they conceptualize and measure EI. All have been shown to have advantages and disadvantages inherent to the type of tool. The instruments reported in the largest number of studies are Emotional Quotient Inventory (EQ-i), Schutte Self Report-Inventory (SSRI), Mayer-Salovey-Caruso Emotional Intelligence Test 2.0 (MSCEIT 2.0), Trait Meta-Mood Scale (TMMS), Wong and Law's Emotional Intelligence Scale (WLEIS), and Trait Emotional Intelligence Questionnaire (TEIQue). The main measure of the estimated reliability has been internal consistency, and the construction of EI measures was predominantly based on linear modelling or classical test theory. The study has limitations: we only searched a single database, the impossibility of estimating inter-rater reliability, and non-compliance with some items required by PRISMA

AXL – a new player in resistance to HER2 blockade

Cancer; HER2 disease; ResistanceCàncer; Malaltia HER2; ResistènciaCáncer; Enfermedad HER2; ResistenciaHER2 is a driver in solid tumors, mainly breast, oesophageal and gastric cancer, through activation of oncogenic signaling pathways such as PI3K or MAPK. HER2 overexpression associates with aggressive disease and poor prognosis. Despite targeted anti-HER2 therapy has improved outcomes and is the current standard of care, resistance emerge in some patients, requiring additional therapeutic strategies. Several mechanisms, including the upregulation of receptors tyrosine kinases such as AXL, are involved in resistance. AXL signaling leads to cancer cell proliferation, survival, migration, invasion and angiogenesis and correlates with poor prognosis. In addition, AXL overexpression accompanied by a mesenchymal phenotype result in resistance to chemotherapy and targeted therapies. Preclinical studies show that AXL drives anti-HER2 resistance and metastasis through dimerization with HER2 and activation of downstream pathways in breast cancer. Moreover, AXL inhibition restores response to HER2 blockade in vitro and in vivo. Limited data in gastric and oesophageal cancer also support these evidences. Furthermore, AXL shows a strong value as a prognostic and predictive biomarker in HER2+ breast cancer patients, adding a remarkable translational relevance. Therefore, current studies enforce the potential of co-targeting AXL and HER2 to overcome resistance and supports the use of AXL inhibitors in the clinic

AKT and JUN are differentially activated in mesenchymal stem cells after infection with human and canine oncolytic adenoviruses

Factor de impacto: 5,987 Q1There is increasing evidence about the use of oncolytic adenoviruses (Ads) as promising immunotherapy agents. We have previously demonstrated the clinical efficiency of mesenchymal stem cells (MSCs) infected with oncolytic Ads as an antitumoral immunotherapy (called Celyvir) in human and canine patients, using ICOVIR-5 or ICOCAV17 as human and canine oncolytic Ads, respectively. Considering the better clinical outcomes of canine patients, in this study we searched for differences in cellular responses of human and canine MSCs to Ad infection that may help understand the mechanisms leading to higher antitumor immune response. We found that infection of human and canine MSCs with ICOVIR-5 or ICOCAV17 did not activate the NF-κB pathway or the interferon regulatory factors IRF3 and IRF7. However, we observed differences in the profile of cytokines secretion, as infection of canine MSCs with ICOCAV17 resulted in lower secretion of several cytokines. Moreover, we showed that infection of human MSCs with ICOVIR-5 increased the phosphorylation of a number of proteins, including AKT and c-JUN. Finally, we demonstrated that differences in regulation of AKT and c-JUN in human and canine MSCs by ICOVIR-5 or ICOCAV17 are intrinsic to each virus. Our findings suggest that ICOCAV17 induces a more limited host response in canine MSCs, which may be related to a better clinical outcome. This result opens the possibility to develop new human oncolytic Ads with these specific properties. In addition, this improvement could be imitated by selecting specific human MSC on the basis of a limited host response after Ad infection.This study was funded by Instituto de Salud Carlos III (PI14CIII/00005 and PI17CIII/00013 grants), Consejería de Educación, Juventud y Deporte of Comunidad de Madrid (P2017/BMD-3692 grant), Fundación Oncohematología Infantil, AFANION, and Asociación Pablo Ugarte, whose support we gratefully acknowledge.S

Toll-like Receptor Signaling-deficient Cells Enhance Antitumor Activity of Cell-based Immunotherapy by Increasing Tumor Homing

Cancer immunotherapy aims to activate the immune system. Some immunotherapeutic agents can be loaded in carrier cells for delivering to the tumors. However, a challenge with cell-based therapies is the selection of the appropriate cells to produce effective clinical outcomes. We hypothesize that therapies based on cells presenting a natural low proinflammatory profile ("silent cells") in the peripheral blood would result in better antitumor responses by increasing their homing to the tumor site. We studied our hypothesis in an immunotherapy model consisting of mesenchymal stromal cells (MSCs) carrying oncolytic adenoviruses for the treatment of immunocompetent mice. Toll-like receptor signaling-deficient cells (TLR4, TLR9, or MyD88 knockout) were used as "silent cells," while regular MSCs were used as control. Although in vitro migration was similar in regular and knockout carrier cells, in vivo tumor homing of silent cells was significantly higher after systemic administration. This better homing to the tumor site was highly related to the mild immune response triggered by these silent cells in peripheral blood. As a result, the use of silent cells significantly improved the antitumor efficacy of the treatment in comparison with the use of regular MSCs. While cancer immunotherapies generally aim to boost local immune responses in the tumor microenvironment, low systemic inflammation after systemic administration of the treatment may indeed enhance their tumor homing and improve the overall antitumor effect. These findings highlight the importance of selecting appropriate donor cells as therapeutic carriers in cell-based therapies for cancer treatment. Cells carrying drugs, virus, or other antitumor agents are commonly used for the treatment of cancer. This research shows that silent cells are excellent carriers for immunotherapies, improving tumor homing and enhancing the antitumor effect.This study was funded by Instituto de Salud Carlos III (grants PI14CIII/00005, PI17CIII/00013, and ISCIII-PFIS FI18CIII/00017), Consejería de Educación, Juventud y Deporte of Comunidad de Madrid (grant P2017/BMD-3692), Fundación Oncohematología Infantil, Asociación Pablo Ugarte and AFANION, whose support we gratefully acknowledge.S

Manejo perioperatorio de fármacos anticoagulantes y antiagregantes en el paciente con fractura de cadera

La fractura de cadera eleva de manera significativa en los estudios publicados la morbilidad y la mortalidad en los pacientes ancianos que la padecen. La participación en el proceso de fármacos que alteran la hemostasia se ha convertido en uno de los aspectos más importantes y controvertidos del manejo perioperatorio del paciente con fractura de cadera. La anticoagulación o antiagregación plaquetaria de estos pacientes tiene unas implicaciones tanto en la práctica anestésica como quirúrgica que hacen necesaria la búsqueda de un equilibrio entre el riesgo hemorrágico y trombótico de los mismos. Con la elaboración del presente protocolo, en línea con las últimas evidencias científicas publicadas, se sientan las bases para avanzar en el tratamiento de este grupo de pacientes. Una atención multidisciplinar especializada, una fijación precoz de la fractura antes de las 48 horas, y un efectivo programa de rehabilitación pueden disminuir las complicaciones del proceso.Hip fracture rises significantly in published studies morbidity and mortality in elderly patients. Participation in the process of hemostasis-altering drugs has become one of the most important and controversial perioperative management of patients with hip fracture aspects. Anticoagulation or antiplatelet of these patients have some implications as a surgical anesthetic practice that make it necessary to seek a balance between bleeding and thrombotic risk thereof. With the development of this protocol, in line with the latest scientific evidence published, the stage is set to advance the treatment of this patient group. A specialized multidisciplinary care, early fracture fixation within 48 hours and an effective rehabilitation program can reduce complications of that process

Delivery of miR-200c-3p Using Tumor-Targeted Mesoporous Silica Nanoparticles for Breast Cancer Therapy

[EN] Despite advancesin breast cancer treatment, it remainsthe leadingcause of cancer-related death in women worldwide. In this context,microRNAs have emerged as potential therapeutic targets but stillpresent some limitations for in vivo applications.Particularly, miR-200c-3p is a well-known tumor suppressor microRNAthat inhibits tumor progression and metastasis in breast cancer throughdownregulating ZEB1 and ZEB2. Basedon the above, we describe the design and validation of a nanodeviceusing mesoporous silica nanoparticles for miR-200c-3p delivery forbreast cancer treatment. We demonstrate the biocompatibility of thesynthesized nanodevices as well as their ability to escape from endosomes/lysosomesand inhibit tumorigenesis, invasion, migration, and proliferationof tumor cells in vitro. Moreover, tumor targetingand effective delivery of miR-200c-3p from the nanoparticles in vivo are confirmed in an orthotopic breast cancer mousemodel, and the therapeutic efficacy is also evidenced by a decreasein tumor size and lung metastasis, while showing no signs of toxicity.Overall, our results provide evidence that miR-200c-3p-loaded nanoparticlesare a potential strategy for breast cancer therapy and a safe andeffective system for tumor-targeted delivery of microRNAs.This research was supported by project PID2021-126304OB-C41 funded by MCIN/AEI/10.13039/501100011033/ and by European Regional Development Fund A way of doing Europe. Also, this study forms part of the Advanced Materials program (MFA/2022/049) and was supported by MCIN with funding from European Union NextGeneration EU(PRTR-C17.I1) and by Generalitat Valenciana. This study was also supported by Generalitat Valenciana (CIPROM/2021/007). This research was also supported by CIBER Consorcio Centro de Investigación Biomédica en Red CIBER-BBN(CB07/01/2012), CIBERONC(CB16/12/00481), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación. This work was also supported by Spanish Government and cofinanced by FEDER Funds (PI18/01219, PI21/01351) .I.G.-C. was funded by Margarita Salas post doctoral grant (European Union-Nextgeneration EU). A.A.-A. and A.L. were funded by Asociación Española Contra el Cancer.J.F.B. was funded by Instituto de Salud Carlos III and the European Social Fund for the financial support 'Sara Borrell' (CD19/00038). V.C.-N. was funded by Ministerio de Ciencia e Innovacio¿n (FPU grant), and J.M.C. was funded by Sociedad Española de Oncología Médica (Río Hortega-SEOM)Garrido-Cano, I.; Adam-Artigues, A.; Lameirinhas, A.; Blandez, JF.; Candela-Noguera, V.; Lluch, A.; Bermejo, B.... (2023). Delivery of miR-200c-3p Using Tumor-Targeted Mesoporous Silica Nanoparticles for Breast Cancer Therapy. ACS Applied Materials & Interfaces. 15(32):38323-38334. https://doi.org/10.1021/acsami.3c075413832338334153