Definizione delle situazioni geomorfologiche rilevanti in due diverse AMP secondo la metodologia CARLIT = Definition of relevant geomorphological situations in two different MPAs with CARLIT methodology

Macroalgae is a biological key element for the assessment of the Ecological Status in coastal waters according to the European Water Framework Directive (2000/60/EC). Here we propose the monitoring of North Sardinia coastal water quality based on CARLIT methodology. A study on the geomorphological factors of two MPAs was made as a first step for the assessment of Reference Conditions fulfils the requirements of the WFD

Mechanism of Isotactic Styrene Polymerization with a C6F5‑Substituted Bis(phenoxyimine) Titanium System

We report a combined, experimental and theoretical, study of styrene polymerization to clarify the regio- and stereocontrol mechanism operating with a C6F5-substituted bis(phenoxyimine) titanium dichloride complex. Styrene homopolymerization, styrene−propene and styrene− ethene−propene copolymerizations have been carried out to this aim. A combination of 13C NMR analysis of the chain-end groups and of the microstructure of the homopolymers and copolymers reveals that styrene polymerization is highly regioselective and occurs prevalently through 2,1- monomer insertion. DFT calculations evidenced that steric interaction between the growing chain and the monomer in the transition state insertion stage is at the origin of this selectivity. The formation of isotactic polystyrene with a chain-end like microstructure is rationalized on the base of a mechanism similar to that proposed for the syndiospecific propene polymerization catalyzed by bis(phenoxyimine) titanium dichloride complexes. Finally, a general stereocontrol mechanism operative in olefin polymerization with this class of complexes is proposed

Can unenhanced MRI of the breast replace contrast-enhanced MRI in assessing response to neoadjuvant chemotherapy?

BACKGROUND: The goals of neoadjuvant chemotherapy (NAC) are to reduce tumor volume and to offer a prognostic indicator in assessing treatment response. Contrast-enhanced magnetic resonance imaging (CE-MRI) is an established method for evaluating response to NAC in patients with breast cancer. PURPOSE: To validate the role of unenhanced MRI (ue-MRI) compared to CE-MRI for assessing response to NAC in women with breast cancer. MATERIAL AND METHODS: Seventy-one patients with ongoing NAC for breast cancer underwent MRI before, during, and at the end of NAC. Ue-MRI was performed with T2-weighted sequences with iterative decomposition of water and fat and diffusion-weighted sequences. CE-MRI was performed using three-dimensional T1-weighted sequences before and after administration of gadobenate dimeglumine. Two blinded observers rated ue-MRI and CE-MRI for the evaluation of tumor response. Statistical analysis was performed to compare lesion size and ADC values changes during therapy, as well as inter-observer agreement. RESULTS: There were no statistically significant differences between ue-MRI and CE-MRI sequences for evaluation of lesion size at baseline and after every cycle of treatment ( P > 0.05). The mean tumor ADC values at baseline and across the cycles of NAC were significantly different for the responder group. CONCLUSION: Ue-MRI can achieve similar results to CE-MRI for the assessment of tumor response to NAC. ADC values can differentiate responders from non-responders

The ability of regional coordination and policy integration to produce coherent marine management : Implementing the Marine Strategy Framework Directive in the North-East Atlantic

The transboundary nature of the marine environment requires concerted actions among neighbouring countries to improve its quality in an effective way. Coordination at international level is particularly important during the implementation of environmental policies aimed at reducing the widespread pressures derived from activities, such as shipping and fishing. The European Marine Strategy Framework Directive (MSFD) aims to protect and improve the status of a wide range of ecosystem components with a regional focus, promoting cooperation among countries and integration with other environmental policies. In 2014, the European Commission assessed the level of adequacy, consistency and coherence achieved by Member States during the implementation of the first phase of the MSFD and hence this paper focuses on the cross-border coherence and coordination within one marine region in order to achieve the goals of the Directive. In particular, it identifies and analyses the main differences among the results of the implementation of the first phase of the MSFD across the North-East Atlantic region. This analysis shows that, in general, the use of existing data, methodologies and targets from related environmental policies corresponds to the higher levels of coherence among countries while a limited use of such policies produces less coherence. This suggests that the European Commission, Regional Seas Conventions and Member States should work together to identify the real connection between the MSFD and other policies to make a proper use of existing data and approaches and to harmonise different policy objectives. In particular, the review shows what might be termed a 'paradox of coherence' amongst Member States where coherence of action has to be achieved within a European policy of subsidiarity, the act of Member States having control over the way they implement framework directives. This can be regarded as a fundamental flaw in having a 'Framework Directive' instead of the greater control in a 'Directive

Can national management measures achieve good status across international boundaries? : a case study of the Bay of Biscay and Iberian coast sub-region

Coastal countries have historically implemented management measures to improve the status of their national marine waters and little effort has been made to take coordinated actions to improve the status of the entire region or sub- region of which they are part. At the European level, the adoption of the Marine Strategy Framework Directive (MSFD) aims to remedy this deficiency and to promote coordination among countries and an integrated management of the marine environment. The MSFD requires each country to propose and adopt a programme of measures to achieve Good Environmental Status of the regional seas. This study compares the programmes of measures of the three countries of the Bay of Biscay and Iberian coast sub-region – France, Portugal and Spain – presenting a novel use of multivariate analyses using semi-quantitative policy information. Among the four North-East Atlantic sub-regions, this study area was chosen because it showed the lowest levels of coherence during the first phase of the implementation of the MSFD, according to the European Commission assessment. The results show the differences among the three programmes, confirming the difficulties that neighbouring countries face when they are required to adopt common approaches in the implementation of this multi-sectoral Directive. Most of the measures developed in the sub-region address marine biodiversity but this is through a wide range of actions, covering different pressures and different species/habitats. The integration with other legislation is more similar between Spain and France and differs between these and Portugal. The three countries also recognise the lack of knowledge to perform the economic analysis, in particular in quantifying the costs of and social benefits derived from their measures. It is concluded here that a better use of the regional and European coordination structures is needed to fill the gaps in knowledge and to exchange good practices. More political will is necessary to take action at European and international level to mitigate the impact of those socio-economic activities through joint programmes, for which Community funding is available

Benefits and impediments for the integrated and coordinated management of European seas

New multi-sectoral policies with a regional implementation are developed when maritime states recognise the importance of managing the marine environment under an ecosystem-perspective rather than a use-perspective. In Europe, the Marine Strategy Framework Directive (MSFD) is the first attempt to promote an integrated management of the seas from the coastline to the limit of the Exclusive Economic Zone. This paper shows that, nine years from the MSFD adoption, there remain several ecological, economic, social and governance challenges. Using information gathered in a dedicated survey of the European Union Marine Strategy Coordination Group and in the recent literature the paper identifies the obstacles preventing a successful regional cooperation and policy integration. The survey indicates that the MSFD coordination structures are, in general, well-developed but there is an apparent lack of political will to coordinate actions at the regional level. Member States request greater flexibility to implement the Directive but they put their national interests before the benefit of a coherent and integrated approach for the entire region. Differences in budget, economic sector predominance, lack of staff and the MSFD short time-scale are identified as the factors that can hamper cooperation. These have produced recommendations of possible strategies for optimising regional coordination structures which respect the subsidiarity principle underpinning the MSFD

Impediments to achieving integrated marine management across borders : The case of the EU Marine Strategy Framework Directive

Several initiatives have been taken worldwide to promote international coordination and integrated approach in marine management. At the European level, ten years after the adoption of the Marine Strategy Framework Directive (MSFD), the Member State strategies still present some ecological, economic and social challenges. This review identifies the minor, intermediate and major impediments (respectively defined as ‘bottlenecks, showstoppers and train-wrecks’) to marine management, resulting from a 4-year analysis of national, regional and European reports. Most of the problems are linked to the resistance of countries to collaborate and to the inability to integrate the work already carried out under other pieces of legislation. The European countries will need to better integrate and coordinate their actions in marine management in the second cycle of the MSFD, in order to achieve its final goal of Good Environmental Status as well as the objectives of other environmental policies

Vaccines against human HER2 prevent mammary carcinoma in mice transgenic for human HER2

INTRODUCTION: The availability of mice transgenic for the human HER2 gene (huHER2) and prone to the development of HER2-driven mammary carcinogenesis (referred to as FVB-huHER2 mice) prompted us to study active immunopreventive strategies targeting the human HER2 molecule in a tolerant host. METHODS: FVB-huHER2 were vaccinated with either IL-12-adjuvanted human HER2-positive cancer cells or DNA vaccine carrying chimeric human-rat HER2 sequences. Onset and number of mammary tumors were recorded to evaluate vaccine potency. Mice sera were collected and passively transferred to xenograft-bearing mice to assess their antitumor efficacy. RESULTS: Both cell and DNA vaccines significantly delayed tumor onset, leading to about 65% tumor-free mice at 70 weeks, whereas mock-vaccinated FVB-huHER2 controls developed mammary tumors at a median age of 45 weeks. In the DNA vaccinated group, 65% of mice were still tumor-free at about 90 weeks of age. The number of mammary tumors per mouse was also significantly reduced in vaccinated mice. Vaccines broke the immunological tolerance to the huHER2 transgene, inducing both humoral and cytokine responses. The DNA vaccine mainly induced a high and sustained level of anti-huHER2 antibodies, the cell vaccine also elicited interferon (IFN)-gamma production. Sera of DNA-vaccinated mice transferred to xenograft-carrying mice significantly inhibited the growth of human HER2-positive cancer cells. CONCLUSIONS: Anti-huHER2 antibodies elicited in the tolerant host exert antitumoral activity

Why amphipods prefer the new available habitat built by C. racemosa? : a field experiment in Mediterranean Sea

The spread of invasive species as Caulerpa racemosa affects the habitat structure in vegetated systems and accumulates high amounts of detritus. Those changes in the habitat cause changes that could affect the invertebrate assemblages. It has been demonstrated that amphipods can actively choose the substrata, so the aim of this study was to respond the question: “will amphipods prefer the new available habitat built by C. racemosa or, on the contrary, is the detritus stock the factor responsible of this changes?” To answer this question experiment of habitat selection in field were carried out. Mesh bags were place in field with 4 different habitats: Halopteris scoparia, Caulerpa racemosa, artificial substrata and empty (control bags). The vegetal content was previously defaunated. For each type of habitat 3 treatments were carried out: high content of detritus, low content of detritus and without detritus (7 replicate samples for each habitat and detritus level).peer-reviewe