Gonad development and reproduction in the monoecious species Chlorophthalmus agassizi (Actinopterygii: Aulopiformes: Chlorophthalmidae) from the Sardinian Waters (Central-Western Mediterranean)

Background. The shortnose greeneye, Chlorophthalmus agassizi Bonaparte, 1840, is a species with a circumglobal distribution and is among the most abundant commercial fishes in some Mediterranean areas. The knowledge of the biology and ecology of this species is poor and geographically limited, then the aim of this study is to provide a contribution to the knowledge on the reproductive biology of this monoecious deep-sea fish in Sardinian waters. Materials and Methods. In this paper the morphology and the development of the gonads, the mean size at maturity, the monthly evolution in the percent frequency of the maturity stages, and of the indices related to reproduction of the shortnose greeneye were examined. Individuals were caught by trawls, between 270 and 504 m of depth in the Sardinian seas. Results. The ovarian pattern is of an asynchronous type, characterized by releasing of eggs in successive batches. Seven stages of development for the ovary and four for the testis were identified on the basis of macroscopic and histological features. The female portion is the most evident component and shows a later maturation than the male portion. The spawning period is unique and takes place from May to September. Conclusion. The identification of spawning period and the adopted reproductive strategy is essential to obtain a better understanding of its biology and a good management of its fisheries

Assessment of alternative land management practices using hydrological simulation and a decision support tool: Arborea agricultural region, Sardinia

Quantifying the impact of land use on water supply and quality is a primary focus of environmental management. In this work we apply a semidistributed hydrological model (SWAT) to predict the impact of different land management practices on water and agricultural chemical yield over a long period of time for a study site situated in the Arborea region of central Sardinia, Italy. The physical processes associated with water movement, crop growth, and nutrient cycling are directly modeled by SWAT. The model simulations are used to identify indicators that reflect critical processes related to the integrity and sustainability of the ecosystem. Specifically we focus on stream quality and quantity indicators associated with anthropogenic and natural sources of pollution. A multicriteria decision support system is then used to develop the analysis matrix where water quality and quantity indicators for the rivers, lagoons, and soil are combined with socio-economic variables. The DSS is used to assess four options involving alternative watersheds designated for intensive agriculture and dairy farming and the use or not of treated wastewater for irrigation. Our analysis suggests that of the four options, the most widely acceptable consists in the transfer of intensive agricultural practices to the larger watershed, which is less vulnerable, in tandem with wastewater reuse, which rates highly due to water scarcity in this region of the Mediterranean. More generally, the work demonstrates how both qualitative and quantitative methods and information can assist decision making in complex settings

Development of sexual organs and fecundity in Octopus vulgaris Cuvier, 1797 from the Sardinian waters (Mediterranean Sea)

In this paper we report information about the sexual maturity process of 245 Octopus vulgaris specimens (75 females and 170 males) from the Mediterranean Sea. For both sexes, six stages of sexual maturity (immature, developing, maturing, mature, spawning, and spent) are identified on the basis of macroscopic and microscopic observations of the reproductive system and linked with some reproductive indices. A good correspondence between gonad appearance and its histological structure is observed, highlighting, in females, how oviducal gland morphology plays a crucial role in the macroscopic evaluation of maturity. The Gonadosomatic and Hayashi indices, in the two genders, and the Oviducal Gland index in females alone do not allow distinguishing all the stages in an irrefutable way. Data on the potential fecundity, oocyte and spermatophore size are reported and compared with literature. In addition, spermatophore components are also computed. The results reported in this paper lead to easy identification of the different phases of sexual maturation of O. vulgaris and could constitute an important tool for defining assessment models in view of sound management of this species

Reproductive patterns in deep versus shallow populations of the precious Mediterranean gorgonian Corallium rubrum ( Linnaeus, 1758) ( Sardinia, Central-Western Mediterranean)

This study quanti es the main reproductive features of the long-lived red coral Corallium rubrum, an octocoral endemic to the Mediterranean Sea and neighbouring Atlantic areas and one of the most valuable of all marine species, at different depths (38-40 m versus 96-115 m) in the north-western Sardinian waters (Central-Western Mediterranean Sea). Different population structures were observed with shallow colonies smaller (in basal diameter and height) than deeper ones. Both populations were all gonochoric at polyp and colony level and fertile. The sex-ratio was balanced in the deep red corals while in the shallow ones it was signi cantly biased towards female. Deep and shallow colonies were found to have a synchronous female sexual products development in the two depth ranges investigated. C. rubrum produced large female sexual products in both depth intervals in relationship with its reproductive mode, con rming a long oogenic development. In contrast to reproductive timing, the volume occupied by female sexual products per polyp was different between the populations, being higher in the deep than in the shallow populations. C. rubrum exhibited a reproductive strategy characterized by a relative low number of female sexual products per polyp revealing signi cant differences among the two depth ranges analyzed with a lower mean value of sexual products in the shallow colonies (mean fecundity per polyp: 1.14) than in the deeper ones (mean fecundity per polyp: 2.09). These results indicate differences in reproductive parameters such as sexual products distribution and reproductive output (fecundity) which were observed between shallow and deep populations of C. rubrum, in particular within deep dwelling populations

Using PVS for Interval Temporal Logic proofs, part 1: The syntactic and semantic encoding

Interval temporal logic (ITL) is a logic that is used to specify and reason about systems. The logic has a powerful proof system but rather than doing proofs by hand, which is tedious and error prone, we want a tool that can check each proof step. Instead of developing a new tool we will use the existing prototype verification system (PVS) as a basic tool. The specification language of PVS is used to encode interval temporal logic semantically and syntactically. With this we can encode the ITL proof system within PVS. Several examples of proofs in ITL that are done per hand are checked with PVS.Funded by EPSRC Research Grant GR/K2592

Differential Modulation of Nitric Oxide Synthases in Aging: Therapeutic Opportunities

Vascular aging is the term that describes the structural and functional disturbances of the vasculature with advancing aging. The molecular mechanisms of aging-associated endothelial dysfunction are complex, but reduced nitric oxide (NO) bioavailability and altered vascular expression and activity of NO synthase (NOS) enzymes have been implicated as major players. Impaired vascular relaxation in aging has been attributed to reduced endothelial NOS (eNOS)-derived NO, while increased inducible NOS (iNOS) expression seems to account for nitrosative stress and disrupted vascular homeostasis. Although eNOS is considered the main source of NO in the vascular endothelium, neuronal NOS (nNOS) also contributes to endothelial cells-derived NO, a mechanism that is reduced in aging. Pharmacological modulation of NO generation and expression/activity of NOS isoforms may represent a therapeutic alternative to prevent the progression of cardiovascular diseases. Accordingly, this review will focus on drugs that modulate NO bioavailability, such as nitrite anions and NO-releasing non-steroidal anti-inflammatory drugs, hormones (dehydroepiandrosterone and estrogen), statins, resveratrol, and folic acid, since they may be useful to treat/to prevent aging-associated vascular dysfunction. The impact of these therapies on life quality in elderly and longevity will be discussed

Spironolactone treatment attenuates vascular dysfunction in type 2 diabetic mice by decreasing oxidative stress and restoring NO/GC signaling

Type 2 diabetes (DM2) increases the risk of cardiovascular disease. Aldosterone, which has pro-oxidative and pro-inflammatory effects in the cardiovascular system, is positively regulated in DM2. We assessed whether blockade of mineralocorticoid receptors (MR) with spironolactone decreases reactive oxygen species (ROS)-associated vascular dysfunction and improves vascular nitric oxide (NO) signaling in diabetes. Leptin receptor knockout [LepRdb/LepRdb (db/db)] mice, a model of DM2, and their counterpart controls [LepRdb/LepR+, (db/+) mice] received spironolactone (50 mg/kg body weight/day) or vehicle (ethanol 1%) via oral per gavage for 6 weeks. Spironolactone treatment abolished endothelial dysfunction and increased endothelial nitric oxide synthase (eNOS) phosphorylation (Ser1177) in arteries from db/db mice, determined by acetylcholine-induced relaxation and Western Blot analysis, respectively. MR antagonist therapy also abrogated augmented ROS-generation in aorta from diabetic mice, determined by lucigenin luminescence assay. Spironolactone treatment increased superoxide dismutase-1 and catalase expression, improved sodium nitroprusside and BAY 41-2272-induced relaxation, and increased soluble guanylyl cyclase (sGC) β subunit expression in arteries from db/db mice. Our results demonstrate that spironolactone decreases diabetes-associated vascular oxidative stress and prevents vascular dysfunction through processes involving increased expression of antioxidant enzymes and sGC. These findings further elucidate redox-sensitive mechanisms whereby spironolactone protects against vascular injury in diabetes