Cirugía micrográfica de Mohs : Estudio retrospectivo de los últimos trece años (1999-2011) en el Hospital de la Santa Creu i Sant Pau

El objetivo de este trabajo fue describir las características de los pacientes tratados con cirugía micrográfica de Mohs entre 1999-2011 y evaluar la eficacia de esta cirugía para el tratamiento de los carcinomas basocelulares. Durante este periodo se realizaron 560 cirugías de Mohs, el 95.36% de las cuales fueron de carcinomas basocelulares. Se observó una tasa de recidiva del 1.40% para los carcinomas basocelulares primarios y del 13.62% para los recurrentes, siendo la diferencia estadísticamente significativa (p 0.0001).L'objectiu d'aquest treball va ser descriure les característiques dels pacients tractats amb cirurgia microgràfica de Mohs entre 1999-2011 i avaluar l'eficàcia d'aquesta cirurgia per al tractament dels carcinomes basocel·lulars. Durant aquest període es van realitzar 560 cirurgies de Mohs, el 95.36% de les quals van ser de carcinomes basocel·lulars. Es va observar una taxa de recidiva del 1.40% per als carcinomes basocel·lulars primaris i del 13.62% per als recurrents, éssent la diferència estadísticament significativa (p 0.0001)

TMT-Based Proteomic Analysis of Human Spermatozoa from Unexplained Recurrent Miscarriage Patients before and after Oral Antioxidant Treatment

Recently, sperm quality and the presence of double-stranded breaks (DSB) has been pointed out as a possible cause of recurrent miscarriage, and the use of antioxidants has expanded as a treatment for male infertility. The aim of the present study was to analyze the proteomic effects of antioxidants on sperm from RM patients with high incidence of DSB. Proteomic analysis was performed using a tandem mass tag labeling technique, and subsequently compared with the PANTHER database for DEPs, and the STRING database for protein-protein interactions (PPI). Differentially expressed proteins (DEPs) both before and after antioxidant oral treatment were identified. PPI involving DEPs clustered into networks related to cell metabolism, cytoskeleton, and DNA damage. Results show that the sperm proteomic profiles before and after antioxidant treatment do not significantly differ from each other. However, some DEPs found after the antioxidant treatment shifted towards a DEPs profile typical of fertile donors. This indirect measurement suggests an improvement caused by antioxidants on the expression of several proteins. Among them were proteins involved in sperm DNA remodeling (LMO7, MMP28, BNC2, H2B, and PRDM2). The results presented here represent the first approach in the analysis and repair of the proteomic change caused by antioxidants in recurrent miscarriage patients, elucidating biomarkers that may be useful for the diagnosis and further sperm selection in this type of patient. Further studies should be conducted to validate the usefulness of these biomarkers in larger study groups

El consum de Netflix i TV3 dels joves a Catalunya

L'objectiu d'aquest treball és fer una anàlisi del consum de la ciutadania jove resident a Catalunya en relació amb Netflix, plataforma líder en servei de vídeo sota demanda a Catalunya, i també respecte a TV3, el canal generalista de televisió de la Corporació Catalana de Mitjans Audiovisuals. Mitjançant grups de discussió, el propòsit és descobrir quins són els motius pels quals hi ha hagut una transformació en la dieta mediàtica dels joves. Els resultats mostren que factors com l'experiència d'usuari, l'accessibilitat i la varietat de continguts són aspectes imprescindibles per tal d'explicar l'increment en l'ús de les plataformes.El objetivo de este trabajo es hacer un análisis del consumo de la ciudadanía joven que reside en Cataluña con relación a Netflix, plataforma líder en servicio de vídeo bajo demanda en Cataluña, y también respecto a TV3, la cadena generalista de televisión de la Corporació Catalana de Mitjans Audiovisuals. Mediante grupos de discusión, el propósito es descubrir cuáles son los motivos por los cuales ha habido una transformación en la dieta mediática de los jóvenes. Los resultados muestran que factores como la experiencia de usuario, la accesibilidad y la variedad de contenidos son aspectos imprescindibles para explicar el incremento en el uso de las plataformas.The aim of this study is to analyze the consumption of young citizens living in Catalonia in relation to Netflix, the leading video on demand service platform in Catalonia, and also to TV3, the generalist television channel of the Corporació Catalana de Mitjans Audiovisuals. Through focus groups, the purpose is to discover what are the reasons why there has been a transformation in the media diet of young people. The results show that factors such as user experience, accessibility and variety of content are essential aspects to explain the increase in the use of platforms

Changing plasma cytokine, chemokine and growth factor profiles upon differing malaria transmission intensities

Background: Malaria epidemiological and immunological data suggest that parasite tolerance wanes in the absence of continuous exposure to the parasite, potentially enhancing pathogenesis. The expansion of control interventions and elimination campaigns raises the necessity to better understand the host factors leading to susceptibility or tolerance that are afected by rapid changes in malaria transmission intensity (MTI). Mediators of cellular immune responses are responsible for the symptoms and pathological alterations during disease and are expected to change rapidly upon malaria exposure or cessation. Methods: The plasma concentrations of 30 cytokine, chemokine and growth factors in individuals of all ages from a malaria endemic area of southern Mozambique were compared between 2 years of diferent MTI: 2010 (lower, n=234) and 2013 (higher, n=143). The efect of the year on the correlations between cytokines, chemokines and growth factors and IgGs to Plasmodium falciparum (markers of exposure) was explored. The efects of age, sex, neighbourhood and parasitaemia on analyte levels and their interactions with year were also assessed. Results: An inverse correlation of several cellular immune mediators with malarial antibodies in 2013, and a lack of correlation or even a positive correlation in 2010 were observed. Most cytokines, chemokines and growth factors, regardless of their immune function, had higher concentrations in 2010 compared with 2013 in P. falciparum-infected and uninfected subjects. Age and neighbourhood showed an efect on analyte concentrations. Conclusions: The results show a diferent regulation of the cellular immune response in 2010 vs 2013 which could be related to a loss of immune-tolerance after a decline in MTI in 2010 and previous years, and a rapid re-establishment of tolerance as a consequence of more continuous exposure as MTI began increasing in 2012. Cellular immune mediators warrant further investigation as possible surrogates of MTI-associated host susceptibility or tolerance

Proteomic Analysis in Seminal Plasma of Fertile Donors and Infertile Patients with Sperm DNA Fragmentation

Seminal plasma proteomics studies could represent a new approach for the determination of molecular elements driving male infertility, resulting in a better male infertility characterization. The aim of this study is to investigate proteomic differences in seminal plasma samples from fertile and infertile individuals. For that, semen samples were selected according to semen analysis, clinical pathology, and values of sperm DNA fragmentation (alkaline and neutral Comet assay and Sperm Chromatin Dispersion test). A total of 24 seminal plasma samples classified in four groups were processed: fertile donors (FD), recurrent miscarriage patients (RM), asthenoteratozoospermic patients (ATZ), and asthenoteratozoospermic patients with varicocele (ATZ-VAR). Results obtained by 2D-differential gel electrophoresis (2D-DIGE) revealed 26 spots significantly increased in fertile donors when compared to patient groups. Also, eight spots in the ATZ group and two in the ATZ-VAR group were decreased compared to the other groups. Twenty-eight proteins were identified by mass spectrometry (MS), most of them involved in metabolic and cellular processes and with a catalytic or binding function. Protein-protein interactions through Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) tool suggest that a large part of them were associated with each other. Furthermore, most of them were associated with ubiquitin C, indicating that it could play an important regulation role, resulting in a potential male infertility biomarker

Analysis of monitoring programmes and their suitability for ecotoxicological risk assessment in four Spanish basins

Data from four Spanish basin management authorities were analysed. Chemical and biological data from four Spanish basin management authorities were analysed, focusing on three consecutive years. Aims were to i) determine the chemicals most likely responsible for the environmental toxicological risk in the four Spanish basins and ii) investigate the relationships between toxicological risk and biological status in these catchments. The toxicological risk of chemicals was evaluated using the toxic unit (TU) concept. With these data we considered if the potential risk properly reflects the risk to the community or, alternatively, if new criteria should be developed to improve risk assessment. Data study revealed inadequacies in processing and monitoring that should be improved (e.g., site coincidence for chemical and biological sampling). Analysis of the chemical data revealed high potential toxicological risk in the majority of sampling points, to which metals were the main contributors to this risk. However, clear relationships between biological quality and chemical risk were found only in one river. Further investigation of metal toxicity may be necessary, and future analyses are necessary to accurately estimate the risk to the environment.The present work was funded by the Spanish Ministry of Economics and Competitiveness through the Consolider-Ingenio 2010 Program (project Scarce CSD2009-00065). The authors would like to acknowledge the Confederacion Hidrografica del Ebro (C. Duran and V. Sanchez-Tello), Confederacion Hidrografica del Guadalquivir (V. Cifuentes), Agenda Andaluza del Agua, Agenda Catalana de l'Aigua (A Munne, L Tirapu) and Confederacion Hidrografica del Jucar (MA Pinon) which kindly provided the monitoring data. Nuria De Castro-Catala holds a predoctoral grant from the University of Barcelona. We would like to thank two anonymous reviewers for their suggestions which have improved this manuscript.López Doval, JC.; De Castro Catala, N.; Andrés Doménech, I.; Blasco, J.; Ginebreda, A.; Muñoz, I. (2012). Analysis of monitoring programmes and their suitability for ecotoxicological risk assessment in four Spanish basins. Science of the Total Environment. 440:194-203. https://doi.org/10.1016/j.scitotenv.2012.07.035S19420344

Genetic landscape of 6089 inherited retinal dystrophies affected cases in Spain and their therapeutic and extended epidemiological implications

Inherited retinal diseases (IRDs), defined by dysfunction or progressive loss of photoreceptors, are disorders characterized by elevated heterogeneity, both at the clinical and genetic levels. Our main goal was to address the genetic landscape of IRD in the largest cohort of Spanish patients reported to date. A retrospective hospital-based cross-sectional study was carried out on 6089 IRD affected individuals (from 4403 unrelated families), referred for genetic testing from all the Spanish autonomous communities. Clinical, demographic and familiar data were collected from each patient, including family pedigree, age of appearance of visual symptoms, presence of any systemic findings and geographical origin. Genetic studies were performed to the 3951 families with available DNA using different molecular techniques. Overall, 53.2% (2100/3951) of the studied families were genetically characterized, and 1549 different likely causative variants in 142 genes were identified. The most common phenotype encountered is retinitis pigmentosa (RP) (55.6% of families, 2447/4403). The most recurrently mutated genes were PRPH2, ABCA4 and RS1 in autosomal dominant (AD), autosomal recessive (AR) and X-linked (XL) NON-RP cases, respectively; RHO, USH2A and RPGR in AD, AR and XL for non-syndromic RP; and USH2A and MYO7A in syndromic IRD. Pathogenic variants c.3386G > T (p.Arg1129Leu) in ABCA4 and c.2276G > T (p.Cys759Phe) in USH2A were the most frequent variants identified. Our study provides the general landscape for IRD in Spain, reporting the largest cohort ever presented. Our results have important implications for genetic diagnosis, counselling and new therapeutic strategies to both the Spanish population and other related populations.This work was supported by the Instituto de Salud Carlos III (ISCIII) of the Spanish Ministry of Health (FIS; PI16/00425 and PI19/00321), Centro de Investigación Biomédica en Red Enfermedades Raras (CIBERER, 06/07/0036), IIS-FJD BioBank (PT13/0010/0012), Comunidad de Madrid (CAM, RAREGenomics Project, B2017/BMD-3721), European Regional Development Fund (FEDER), the Organización Nacional de Ciegos Españoles (ONCE), Fundación Ramón Areces, Fundación Conchita Rábago and the University Chair UAM-IIS-FJD of Genomic Medicine. Irene Perea-Romero is supported by a PhD fellowship from the predoctoral Program from ISCIII (FI17/00192). Ionut F. Iancu is supported by a grant from the Comunidad de Madrid (CAM, PEJ-2017-AI/BMD7256). Marta del Pozo-Valero is supported by a PhD grant from the Fundación Conchita Rábago. Berta Almoguera is supported by a Juan Rodes program from ISCIII (JR17/00020). Pablo Minguez is supported by a Miguel Servet program from ISCIII (CP16/00116). Marta Corton is supported by a Miguel Servet program from ISCIII (CPII17/00006). The funders played no role in study design, data collection, data analysis, manuscript preparation and/or publication decisions

Marco Conceptual y Estado del Arte sobre la aplicación del blockchain en la Gestión de la Cadena de Suministro

[ES] En los últimos años ha crecido el interés en la aplicación de la tecnología blockchain en la Gestión de la cadena de suministro. El número de artículos publicados ha ido de la mano de este creciente interés, surgiendo así la necesidad de analizar y englobar las últimas investigaciones publicadas sobre la aplicación de dicha tecnología. El presente trabajo presenta una revisión literaria de las últimas aplicaciones del sistema blockchain en la gestión de las distintas cadenas de suministro (SCM). Para ello en primer lugar se realiza un estudio de las revisiones literarias actuales existentes para posteriormente proponer un marco conceptual propio y novedoso donde poder clasificar las últimas publicaciones de aplicación de la tecnología blockchain en la gestión de la cadena de suministro. El propósito de la revisión es obtener una visión general actual de las innovaciones que se han propuesto en este contexto para que sirvan de guía y de inspiración para futuras investigaciones de aplicación.[EN] In recent years interest has grown in the application of blockchain technology in the management of the supply chain. The number of published articles has gone hand in hand with this growing interest, thus emerging the need to analyze and encompass the latest published research on the application of such technology. The present work presents a literary review of the latest applications of the blockchain system in the management of the different supply chains (SCM). First of all, a study of existing literary reviews is carried out to later propose a novel and original conceptual framework in which to classify the latest publications on the application of blockchain technology in the supply chain management. The purpose of the review is to obtain a current overview of the innovations that have been proposed in this context to serve as a guide and inspiration for future application research.[CA] En els últims anys ha crescut l’interés en l’aplicació de la tecnologia blockchain en la Gestió de la cadena de subministrament. El nombre d’articles publicats ha anat de la mà d’aquest creixent interés, sorgint així la necessitat d’analitzar i englobar les últimes investigacions publicades sobre l’aplicació d’aquesta tecnologia. El present treball presenta una revisió literària de les últimes aplicacions del sistema blockchain en la gestió de les diferents cadenes de subministrament (GCdS). Per a això en primer lloc es realitza un estudi de les revisions literàries actuals existents per a posteriorment proposar un marc conceptual i així poder realitzar una classificació exhaustiva i sistemàtica de les publicacions d’aplicació de la tecnologia blockchain en la gestió de la cadena de subministrament. El propòsit de la revisió és obtindre una visió general actual de les innovacions que s’han proposat en aquest context perquè servisquen de guia i d’inspiració per a futures investigacions d’aplicació.Bascuñana Català, A. (2019). Marco Conceptual y Estado del Arte sobre la aplicación del blockchain en la Gestión de la Cadena de Suministro. http://hdl.handle.net/10251/12859