Evaluation of the anti-angiogenic potential of hydroxytyrosol derivatives

Angiogenesis, a process which allows the formation of new vessels from pre-existing ones, is an essential phenomenon for tumor survival since it allows cancer cells to obtain nutrients and oxygen. This explains the increasing interest showed by many groups of research and pharmaceutical companies to find compounds with potential to disrupt at least one of the steps within the angiogenic process. Hydroxytyrosol (3,4-dihydroxyphenyl ethanol) has been identified as the most important health-related phenolic compound of virgin olive oil because of its pleiotropic effects on multiple targets. In 2012, our group identified hydroxytyrosol as an anti-angiogenic compound able to inhibit several key steps in the angiogenic process. In the present study, the potential effects of six hydroxytyrosol derivatives are tested and compared with those exhibited by hydroxytyrosol by making use of several in vitro and in vivo assays. Results indicate that these are candidate new anti-angiogenic compounds with potential utility in anti-tumor and anti-angiogenic therapies.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech [Our experimental work is supported by grants BIO2014-56092-R (MINECO and FEDER) and P12-CTS-1507 (Andalusian Government and FEDER) and funds from group BIO-267 (Andalusian Government). The "CIBER de Enfermedades Raras" is an initiative from the ISCIII (Spain)]. This communication has the support of a travel grant

Initial pH as a determining factor of glucose consumption and lactic and acetic acid production in oral streptococci

Lactic and acetic acid production was evaluated from six strains of oral streptococci, viz Streptococcus gordonii, Streptococcus mutans, Streptococcus oralis, Streptococcus salivarius, Streptococcus sanguis and Streptococcus sobrinus cultured in the presence of 1, 5, 10 mM glucose and without glucose, at initial pH values of 5, 5.5, 6 and 7. S. sobrinus and S. salivarius caused the greatest decreases in pH. At pH values of 5 and 5.5, lactic acid and acetic acid production in the species tested was discordant with residual glucose levels. Acid production from protein was especially great in S. mutans and S. salivarius

Action of sodium fluoride on phagocytosis by systemic polymorphonuclear leucocytes

This study was supported in part by the Education and Sciences Council of the Andalusian Regional Government through Project nº 082.Alterations in phagocytosis appear to be important in the onset and development of periodontitis. We investigated new substances that may be of use in the treatment of periodontitis. In a preliminary study, we tested the effect of sodium fluoride on phagocytosis by circulating polymorphonuclear leucocytes (PMNs) in 10 replicate assays using blood from six healthy subjects. Sodium fluoride was tested at concentrations of 1.0 micron to 4.0 microns against Streptococcus oralis, Streptococcus mutans, Streptococcus sobrinus and Streptococcus sanguis. The phagocytic index against all microorganisms increased significantly at all concentrations of sodium fluoride assayed; this effect was dose dependent. Sodium fluoride appeared to stimulate phagocytosis via two mechanisms: an apparent increase in bacterial susceptibility to phagocytosis, and direct stimulation of phagocytosis by PMNs.Andalusian Regional Governmen

Baseline to Evaluate Off-Site Suspended Sediment-Related Mining Effects in the Alto Tajo Natural Park, Spain

Mining is a human activity with considerable environmental impact. To evaluate such impacts, international laws require undertaking local studies based on direct sampling to establish baseline conditions of parameters modified by human activities. Mining takes place near the Alto Tajo Natural Park, where a suspended sediment concentration (SSC) baseline is required to determine whether mining affects water quality. To this end, we have monitored the Tajo River and its tributary the Tajuelo following Before–After Control-Impact (BACI) techniques, recommended by Australian and New Zealand laws, requiring a specific method based on continuous monitoring and sampling to enable evaluation of SSCs. An SSC baseline has been defined at stations situated upstream of the mining area and compared with those downstream. The highest detected SSC upstream of the Tajuelo mines was 24 g l−1 whereas the highest simultaneous downstream value was 391 g l−1, more than one order of magnitude higher than the supposed baseline (24 g l−1). Additionally, this value is 1000 times more than the average concentration of 25 mg l−1, used by the European Union until 2015, to guarantee the quality of salmonid waters. Following a BACI approach, a statistically significant SSC impact has been identified. The mined areas are the only source that can explain this increase. This is the first instance that such an increase and baseline have been found using this method. BACI is a simple and reliable method recommended for studying degraded areas rather than an irrelevant, fixed standard as included in most international laws. Copyright © 2016 John Wiley & Sons, Ltd.This study was funded by: (i) Research Project CGL2010-21754-C02-01 (Spanish Ministry of Science and Technology, and Ministry of Economy); (ii) the Department of Agriculture and Environment of Castile—La Mancha through its socalled “Fondos Verdes” (Green Funding); (iii) the research contract 249/2015 between CAOBAR and UCM; (iv) the Ecological Restoration Network REMEDINAL-3 of the Madrid Community (S2013/MAE-2719); (v) the Spanish National Research Council (CSIC) for Ph.D. Grant JAEPredoc to IZAPeer reviewe

Is adenosine deaminase (ADA) activity in saliva and serum a more accurate disease detection tool than traditional redox balance parameters in early-lactating dairy cows?

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.Enzyme adenosine deaminase (ADA) is a marker of inflammation in domestic animals, but it is unclear whether it is a reliable marker of oxidative stress, especially in the transition period in dairy cows. This study aims to assess if ADA and redox status measurements in saliva provide the same utility to detect disease condition as that obtained from serum. Sixtyeight multiparous Holstein cows, between 2 and 3 weeks postpartum were selected. Five study groups were established: control (healthy), and cows with ketosis, mastitis, laminitis, and metritis. The parameters measured were ADA activity, total oxidants (TOS), antioxidants (TAC), and OSi ratio. Regarding redox status, no significant differences arise in both saliva and serum being the correlations negative and not significant. In saliva, ADA activity in healthy cows differs from those with pathological processes, having the lowest activities. In serum, ADA activity is similar in the healthy and ketosis cows, showing the lowest activities meanwhile animals with mastitis, laminitis, or metritis have significantly higher activities. In conclusion, the measurement of ADA activities and redox status in saliva does not give consistent results, being preferable to measure them in serum during the transition period

Valoración microclimática de propuestas de urbanización sustentable : El caso del piedemonte del área metropolitana de Mendoza

El desarrollo urbano del piedemonte del Área Metropolitana de Mendoza ha continuado con las  características  urbanas  de llanura, generando un impacto negativo sobre los  perfiles higrotérmicos  del sector. Por ello, se diseñaron un conjunto de esquemas  urbanos  orientados  a disminuir los  riesgos  naturales  y  preservar las  características  ambientales  del sector, con el fin de mitigar el efecto de las urbanizaciones sobre el microclima. Para verificar si las propuestas mejoran el comportamiento térmico de las  urbanizaciones  actuales, un esquema urbano existente y  dos esquemas de urbanizaciones adaptadas a condiciones de piedemonte fueron simulados utilizando el software ENVI-met. Los  resultados  muestran que el esquema urbano orgánico de tipo lineal con espacio verde público posee el mejor comportamiento térmico, logrando una  disminución de la temperatura máxima del aire de hasta 1,5°C con respecto al esquema urbano existente. Además, se pone en relevancia los beneficios de utilizar ENVI-met como herramienta de simulación, dado que se verifica que el modelo reproduce de manera confiable los principales procesos de cambio atmosféricos que afectan al microclima, dando valor a las predicciones que derivan de su uso. Esto facilita la labor de los  planificadores  urbanos  optimizando los  procesos  de toma de decisión durante la etapa de diseño.Urban development in the hillsides of the Metropolitan Area of Mendoza has continued urban characteristics of plain, generating a negative impact on the hygrothermal profiles of the sector. For this reason, a set of urban schemes were designed aimed at reducing natural hazards and preserving the environmental characteristics of the sector, in order to mitigate the effect of urbanization on the microclimate. To verify whether the proposals improve the thermal behavior of current developments, an existing urban scheme and two urbanization schemes adapted to hillside conditions were simulated using the ENVI-met software. The results show that the organic lineal urban scheme type with public green space has the best thermal behavior, achieving a decrease in the maximum air temperature of up to 1.5 °C with respect to the existing urban scheme. Also, the benefits of using ENVI-met as a simulation tool are highlighted, since it is verified that the model reliably reproduces the main atmospheric change processes that affect the microclimate, giving value to the predictions derived from its use. This facilitates the work of urban planners optimizing decision-making processes during the design stage.Asociación Argentina de Energías Renovables y Medio Ambiente (ASADES

Leiosaurus jaguaris Laspiur, Acosta & Abdala, 2007 (Squamata: Leiosauridae): Primer registro para el Parque Nacional Talampaya, provincia de La Rioja, Argentina

Localidad.- Argentina, provincia de La Rioja, Departamentos de General Felipe Varela e Independencia, Parque Nacional Talampaya (29°47’38.5’’S, 67°59’05.8’’W). Fecha: 4 de marzo de 2020. Avistado y fotografiado por Lucía Martínez Retta, Victor M. Pardo y Ana C. Ochoa. Fotografías depositadas en la Colección Herpetológica de la Fundación Miguel Lillo (FML 30834).Asociación Herpetológica Argentin

COVID Obesity: A One-Year Narrative Review

On 11 March 2020, coronavirus disease 2019 (COVID-19) was declared a pandemic by the World Health Organization (WHO). This study focuses on a narrative review about the illness during the first year of the pandemic in relation to obesity. Databases were used to search studies published up to 8 December 2020. In total, 4430 articles and other scientific literature were found, and 24 articles were included in this one-year narrative review. The mean BMI value of severe COVID-19 patients ranged from 24.5 to 33.4 kg/m2, versus <18.5 to 24.3 kg/m2 for non-severe patients. Articles using the terms obesity or overweight without indicating the BMI value in these patients were common, but this is not useful, as the anthropometric parameters, when not defined by this index, are confusing due to the classification being different in the West compared to among Asian and Korean criteria-based adults. We proposed a new term, called COVID obesity, to define the importance of this anthropometric parameter, among others, in relation with this pandemic

Brain cortical maturation assessed by magnetic resonance imaging in unaffected/mildly affected fetuses with cytomegalovirus infection

Objectives: To assess by magnetic resonance imaging (MRI) the cortical maturation pattern in fetuses with cytomegalovirus (CMV) infection with mild or no abnormalities on ultrasound (US) and MRI, and to establish possible differences compared with healthy controls. Methods: This was a retrospective case-control study of consecutive pregnancies with a CMV-infected fetus undergoing prenatal MRI as a complementary diagnostic tool in two centers, and a control group of singleton low-risk pregnancies without fetal structural abnormalities, with normal fetal growth and with healthy newborns. CMV infection was confirmed by extraction of CMV-DNA from fetal and neonatal samples. Only fetuses with mild (mildly affected) or no (unaffected) neuroimaging abnormalities on US and MRI were included. MRI measurements of fetal parieto-occipital sulcus, cingulate sulcus and calcarine sulcus depth, Sylvian fissure depth and Sylvian fissure angles were performed and cortical development grading of specific cortical areas and sulci were assessed by one operator who was blinded to CMV infection status. Data were compared between controls and fetuses with CMV infection, using linear regression and non-parametric trend analysis. Results: Twenty-four CMV-infected fetuses (seven unaffected and 17 mildly affected) and 24 healthy controls that underwent fetal MRI between 27 and 36 weeks' gestation were included. Compared with controls, CMV-infected fetuses showed significantly larger median lateral ventricular width (right side, 7.8 (interquartile range (IQR), 5.9-9.9) mm vs 3.9 (IQR, 2.6-5.3) mm; left side, 7.5 (IQR, 6.0-10.9) mm vs 4.2 (IQR, 3.2-5.3) mm), significantly decreased parieto-occipital sulcus depth (right side, 12.6 (IQR, 11.3-13.5) mm vs 15.9 (IQR, 13.5-17.3) mm; left side, 12.3 (IQR, 10.6-13.5) mm vs 16.0 (IQR, 13.3-17.5) mm) and calcarine sulcus depth (right side, 15.4 (IQR, 14.4-16.3) mm vs 17.5 (IQR, 16.1-18.7) mm; left side, 14.6 (IQR, 14.1-15.6) mm vs 16.7 (IQR, 15.6-18.9) mm) (P < 0.001 for all). Compared with controls, CMV-infected fetuses also had significantly smaller upper (right side, 42.8° (IQR, 35.8-45.8°) vs 48.9° (IQR, 38.4-64.7°); left side, 40.9° (IQR, 34.2-45.8°) vs 48.2° (IQR, 41.9-60.7°)) and lower (right side, 41.6° (IQR, 34.4-49.2°) vs 48.9° (IQR, 40.6-60.9°); left side, 42.2° (IQR, 38.8-46.9°) vs 48.9° (IQR, 39.5-57.5°)) Sylvian fissure angles (P < 0.05 for all). In addition, the mildly affected CMV-infected fetuses had a significantly lower cortical development grading in the temporal and parietal areas, and the parieto-occipital and calcarine sulci compared with healthy fetuses (P < 0.05). These differences persisted when adjusting for gestational age, ipsilateral atrium width, fetal gender and when considering small-for-gestational age as a confounding factor. Conclusions: Unaffected and mildly affected CMV-infected fetuses showed delayed cortical maturation compared with healthy controls. These results suggest that congenital CMV infection, even in non-severely affected fetuses that are typically considered of good prognosis, could be associated with altered brain cortical structure. Further research is warranted to better elucidate the correlation of these findings with neurodevelopmental outcomes. © 2022 The Authors. Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology