Autism-associated SNPs in the clock genes _npas2_, _per1_ and the homeobox gene _en2_ alter DNA sequences that show characteristics of microRNA genes.

Intronic single nucleotide polymorphisms (SNPs) in the clock genes _npas2_ and _per1_ and the homeobox gene _en2_ are reported to be associated with autism. This bioinformatics analysis of the intronic regions which contain the autism-associated SNPs rs1861972 and rs1861973 in _en2_, rs1811399 in _npas2_, and rs885747 in _per1_, shows that these regions encode RNA transcripts with predicted structural characteristics of microRNAs. These microRNA-like structures are disrupted _in silico_ by the presence of the autism enriched alleles of rs1861972, rs1861973, rs1811399 and rs885747 specifically, as compared with the minor alleles of these SNPs. The predicted gene targets of these microRNA-like structures include genes reported to be implicated in autism (_gabrb3_, _shank3_) and genes causative of diseases co-morbid with autism (_mecp2_ and _rai1_). The inheritance of the AC haplotype of rs1861972 - rs1861973 in _en2_, the C allele of rs1811399 in _npas2_, and the C allele of rs1234747 in _per1_ may contribute to the causes of autism by affecting microRNA genes that are co-expressed along with the homeobox gene _en2_ and the circadian genes _npas2_ and _per1_

Heterogeneity of Intrinsic and Synaptic Properties of Neurons in the Ventral and Dorsal Parts of the Ventral Nucleus of the Lateral Lemniscus

The ventral nucleus of the lateral lemniscus (VNLL) provides a major inhibitory projection to the inferior colliculus (IC). Neurons in the VNLL respond with various firing patterns and different temporal precision to acoustic stimulation. The present study investigates the underlying intrinsic and synaptic properties of various cell types in different regions of the VNLL, using in vitro electrophysiological recordings from acute brain slices of mice and immunohistochemistry. We show that the biophysical membrane properties and excitatory input characteristics differed between dorsal and ventral VNLL neurons. Neurons in the ventral VNLL displayed an onset-type firing pattern and little hyperpolarization-activated current (Ih). Stimulation of lemniscal inputs evoked a large all-or-none excitatory response similar to Calyx of Held synapses in neurons in the lateral part of the ventral VNLL. Neurons that were located within the fiber tract of the lateral lemniscus, received several and weak excitatory input fibers. In the dorsal VNLL onset-type and sustained firing neurons were intermingled. These neurons showed large Ih and were strongly immunopositive for the hyperpolarization- activated cyclic nucleotide-gated channel 1 (HCN1) subunit. Both neuron types received several excitatory inputs that were weaker and slower compared to ventrolateral VNLL neurons. Using a mouse model that expresses channelrhodopsin under the promotor of the vesicular GABA transporter (VGAT) suggests that dorsal and ventral neurons were inhibitory since they were all depolarized by light stimulation. The diverse membrane and input properties in dorsal and ventral VNLL neurons suggest differential roles of these neurons for sound processing

Impact of fracture clustering on the seismic signatures of porous rocks containing aligned fractures

The presence of fractures in a reservoir can have a significant impact on its effective mechanical and hydraulic properties. Many researchers have explored the seismic response of fluid-saturated porous rocks containing aligned planar fractures through the use of analytical models. However, these approaches are limited to the extreme cases of regular and uniform random distributions of fractures. The purpose of this work is to consider more realistic distributions of fractures and to analyze whether and how the frequency-dependent anisotropic seismic properties of the medium can provide information on the characteristics of the fracture network. Particular focus is given to fracture clustering effects resulting from commonly observed fracture distributions. To do so, we have developed a novel hybrid methodology combining the advantages of 1D numerical oscillatory tests, which allows us to consider arbitrary distributions of fractures, and an analytical solution that permits extending these results to account for the effective anisotropy of the medium. A corresponding numerical analysis indicates that the presence of clusters of fractures produces an additional attenuation and velocity dispersion regime compared with that predicted by analytical models. The reason for this is that a fracture cluster behaves as an effective layer and the contrast with respect to the unfractured background produces an additional fluid pressure diffusion length scale. The characteristic frequency of these effects depends on the size and spacing between clusters, the latter being much larger than the typical spacing between individual fractures. Moreover, we find that the effects of fracture clustering are more pronounced in attenuation anisotropy than velocity anisotropy data. Our results indicate that fracture clustering effects on fluid pressure diffusion can be described by two-layer models. This, in turn, provides the basis for extending current analytical models to account for these effects in inversion schemes designed to characterize fractured reservoirs from seismic data.Fil: Barbosa, Nicolás D.. Universite de Lausanne; SuizaFil: Rubino, Jorge German. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Caspari, Eva. Universite de Lausanne; SuizaFil: Holliger, Klaus. Universite de Lausanne; Suiz

Shot-noise measurements of single-atom junctions using a scanning tunneling microscope

Current fluctuations related to the discreteness of charge passing through small constrictions are termed shot noise. This unavoidable noise provides both advantages—being a direct measurement of the transmitted particles’ charge—and disadvantages—a main noise source in nanoscale devices operating at low temperature. While better understanding of shot noise is desired, the technical difficulties in measuring it result in relatively few experimental works, especially in single-atom structures. Here, we describe a local shot-noise measurement apparatus and demonstrate successful noise measurements through single-atom junctions. Our apparatus, based on a scanning tunneling microscope, operates at liquid helium temperatures. It includes a broadband commercial amplifier mounted in close proximity to the tunnel junction, thus reducing both the thermal noise and input capacitance that limit traditional noise measurements. The full capabilities of the microscope are maintained in the modified system, and a quick transition between different measurement modes is possible

Fluid pressure diffusion effects on the seismic reflectivity of a single fracture

When seismic waves travel through a fluid-saturated porous medium containing a fracture, fluid pressure gradients are induced between the compliant fracture and the stiffer embedding background. The resulting equilibration through fluid pressure diffusion (FPD) produces a frequency dependence of the stiffening effect of the fluid saturating the fracture. As the reflectivity of a fracture is mainly controlled by the stiffness contrast with respect to the background, these frequency-dependent effects are expected to affect the fracture reflectivity. The present work explores the P- and S-wave reflectivity of a fracture modeled as a thin porous layer separating two half-spaces. Assuming planar wave propagation and P-wave incidence, this article analyzes the FPD effects on the reflection coefficients through comparisons with a low-frequency approximation of the underlying poroelastic model and an elastic model based on Gassmann's equations. The results indicate that, while the impact of global flow on fracture reflectivity is rather small, FPD effects can be significant, especially for P-waves and low incidence angles. These effects get particularly strong for very thin and compliant, liquid-saturated fractures and embedded in a high-permeability background. In particular, this study suggests that in common environments and typical seismic experiments FPD effects can significantly increase the seismic visibility of fractures.Fil: Barbosa, Nicolás D.. Universite de Lausanne; SuizaFil: Rubino, Jorge German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina. University of Western Ontario; CanadáFil: Caspari, Eva. Universite de Lausanne; SuizaFil: Milani, Marco. Universite de Lausanne; SuizaFil: Holliger, Klaus. Universite de Lausanne; Suiz

Seismic Signatures of Fractured Porous Rocks: The Partially Saturated Case

Seismic attenuation and phase velocity dispersion due to mesoscopic fluid pressure diffusion (FPD) have received increasing attention due to their inherent sensitivity to the hydromechanical properties of monosaturated fractured porous media. While FPD processes are directly affected by key macroscopic properties of fractured rocks, such as fracture density and fracture connectivity, there is, as of yet, a lack of comprehension of the associated characteristics when multiple immiscible phases saturate the probed fractured medium. In this work, we analyze the variations experienced by P and S wave attenuation and phase velocity dispersion when CO2 percolates into an initially brine-saturated fractured porous rock. We study such variations considering a simple model of a porous rock containing intersecting orthogonal fractures as well as a more complex model comprising a fracture network. In the latter, we simulate the flow of a CO2 plume into the medium using an invasion percolation procedure. Representative samples are subjected to numerical upscaling experiments, consisting of compression and shear tests, prior to and after the CO2 invasion process. Results show that fracture-to-background FPD is only sensitive to the presence of CO2, which decreases its effects. However, fracture-to-fracture FPD depends on both the overall CO2 saturation and the fluid distribution within the fracture network. While the former modulates the magnitude of the dissipation, the latter can give rise to a novel FPD process occurring between CO2-saturated and brine-saturated regions of the fracture network.Fil: Solazzi, Santiago Gabriel. Universite de Lausanne; Suiza. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hunziker, Jürg. Universite de Lausanne; SuizaFil: Caspari, Eva. Universite de Lausanne; Suiza. Montanuniversität Leoben; AustriaFil: Rubino, Jorge German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; ArgentinaFil: Favino, Marco. Universite de Lausanne; SuizaFil: Holliger, Klaus. Universite de Lausanne; Suiza. Zhejiang University; República de Chin

Influence of dielectric thickness and electrode structure on the ion wind generation by micro fabricated plasma actuators

Surface dielectric barrier discharges are investigated in order to explore the combined effects of barrier thickness and microstructure of the exposed electrode on the ion wind generation. Actuators with straight and structured high voltage electrodes with characteristic sizes of 200 and 250 µm and dielectric thicknesses of 0.5, 1 and 2 mm are compared. It is observed that: i) actuator efficiency of ion wind generation strongly depends on the applied voltage amplitude; ii) operation voltage depends on the dielectric thickness logarithmically; iii) electrode microstructure slightly increases the dynamic pressure (few percent in maximum), however the effect decreases with thicker dielectrics and smaller electrode structures; iv) the pattern of the most intensive discharge parts as well as the dielectric erosion repeats the regular structure of the electrodes down to 200 µm. Several identical samples are tested during different days to estimate the impact of the air humidity and the degradation of the dielectric. The microscale precision of the sample manufacture was accomplished by a commercial facility for printed circuit boards. © 2020 The Author(s). Published by IOP Publishing Ltd

Impact of poroelastic effects on the inversion of fracture properties from amplitude variation with offset and azimuth data in horizontal transversely isotropic media

<jats:p> The identification and characterization of fractures is an important objective in many areas of earth and environmental sciences. Amplitude variation with offset and azimuth (AVOAz) analysis of seismic reflection data is a key method for achieving these tasks. Theoretical and experimental studies have shown that the presence of pore fluids together with the strong mechanical contrast between the fractures and their embedding background give rise to wave-induced fluid flow (WIFF) effects. This implies that the effective stiffness tensor of a fluid-saturated fractured rock defining its seismic response becomes viscoelastic and frequency-dependent. In spite of this, AVOAz analysis typically relies on end-member-type elastic stiffness models that either assume a relaxed (i.e., equilibrated) or unrelaxed (i.e., unequilibrated) state of the wave-induced fluid pressure in the rock. In general, however, neither the appropriateness of the chosen model nor the associated errors in the inversion process are known. To investigate this topic, we have considered a poroelastic medium containing parallel vertical fractures and generate synthetic seismic AVOAz data using the classic Rüger approximations for PP-wave reflection coefficients in horizontally transversely isotropic media. A Markov chain Monte Carlo method is used to perform a Bayesian inversion of the synthetic seismic AVOAz data. We quantify the influence of WIFF effects on the AVOAz inversion results when elastic relaxed and unrelaxed models are used as forward solvers of inversion schemes to estimate the fracture volume fraction, the elastic moduli, and the porosity of the background rock, as well as the overall weakness of the medium due to the presence of fractures. Our results indicate that, when dealing with single-frequency data, relaxed elastic models provide biased but overall better inversion results than unrelaxed ones, for which some fracture parameters cannot be resolved. Improved inversion performance is achieved when using frequency-dependent data, which illustrates the importance of accounting for poroelastic effects. </jats:p&gt

Estimation of Fracture Compliance From Attenuation and Velocity Analysis of Full‐Waveform Sonic Log Data

In fractured rocks, the amplitudes of propagating seismic waves decay due to various mechanisms, such as geometrical spreading, solid friction, displacement of pore fluid relative to the solid frame, and transmission losses due to energy conversion to reflected and transmitted waves at the fracture interfaces. In this work, we characterize the mechanical properties of individual fractures from P wave velocity changes and transmission losses inferred from static full‐waveform sonic log data. The methodology is validated using synthetic full‐waveform sonic logs and applied to data acquired in a borehole penetrating multiple fractures embedded in a granodioritic rock. To extract the transmission losses from attenuation estimates, we remove the contributions associated with other loss mechanisms. The geometrical spreading correction is inferred from a joint analysis of numerical simulations that emulate the borehole environment and the redundancy of attenuation contributions other than geometrical spreading in multiple acquisitions with different source‐receiver spacing configurations. The intrinsic background attenuation is estimated from measurements acquired in the intact zones. In the fractured zones, the variations with respect to the background attenuation are attributed to transmission losses. Once we have estimated the transmission losses associated with a given fracture, we compute the transmission coefficient, which, on the basis of the linear slip theory, can then be related to the mechanical normal compliance of the fracture. Our results indicate that the estimated mechanical normal compliance ranges from 1 × 10−13 to 1 × 10−12 m/Pa, which, for the size of the considered fractures, is consistent with the experimental evidence available.Fil: Barbosa, Nicolás D.. Universite de Lausanne; Suiza. Universidad de Ginebra; SuizaFil: Caspari, Eva. Universite de Lausanne; SuizaFil: Rubino, Jorge German. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro. Archivo Histórico del Centro Atómico Bariloche e Instituto Balseiro | Universidad Nacional de Cuyo. Instituto Balseiro. Archivo Histórico del Centro Atómico Bariloche e Instituto Balseiro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; ArgentinaFil: Greenwood, Andrew. Universite de Lausanne; SuizaFil: Baron, Ludovic. Universite de Lausanne; SuizaFil: Holliger, Klaus. Universite de Lausanne; Suiza. Zhejiang University; Chin

Representative elementary volumes for evaluating effective seismic properties of heterogeneous poroelastic media

Understanding and quantifying seismic energy dissipation in fluid-saturated porous rocks is of considerable interest because it offers the perspective of extracting information with regard to the elastic and hydraulic rock properties. An important, if not dominant, attenuation mechanism prevailing in the seismic frequency band is wave-induced fluid pressure diffusion in response to the contrasts in elastic stiffness in the mesoscopic-scale range. An effective way to estimate seismic velocity dispersion and attenuation related to this phenomenon is through the application of numerical upscaling procedures to synthetic rock samples of interest. However, the estimated seismic properties are meaningful only if the underlying sample volume is at least of the size of a representative elementary volume (REV). In the given context, the definition of an REV and the corresponding implications for the estimation of the effective seismic properties remain largely unexplored. To alleviate this problem, we have studied the characteristics of REVs for a set of idealized rock samples sharing high levels of velocity dispersion and attenuation. For periodically heterogeneous poroelastic media, the REV size was driven by boundary condition effects. Our results determined that boundary condition effects were absent for layered media and negligible in the presence of patchy saturation. Conversely, strong boundary condition effects arose in the presence of a periodic distribution of finite-length fractures, thus leading to large REV sizes. The results thus point to the importance of carefully determining the REV sizes of heterogeneous porous rocks for computing effective seismic properties, especially in the presence of strong dry frame stiffness contrasts. </jats:p