Os princípios do Direito Internacional: uma fonte de direito ou de uma fonte de perguntas?

The aim of this work is to contribute to the legal discussion whit an analysis in which normative categories converges that allow to dialogue both constitutionalists, internationalists and law theorists in the analysis of nuclear concepts in the relationship between national law and international law, Specifically in the role that plays the Principles in International Law and in the Normative Systems.El objetivo de este trabajo es aportar a la discusión jurídica mediante un análisis en el que converjan categorías normativas que permitan dialogar tanto a constitucionalistas, internacionalistas y teóricos del Derecho para el estudio de conceptos nucleares en la relación entre Derecho nacional y Derecho Internacional, específicamente en el rol que le corresponde desempeñar a los Principios en el Derecho Internacional y en el Derecho.  O objetivo deste trabalho é contribuir para a discussão jurídica de uma análise em que uma mistura de categorias políticas que permitem diálogo tanto constitucionalistas, internacionalistas e teóricos da lei para o estudo dos conceitos nucleares sobre a relação entre o direito nacional e direito internacional, especificamente para o papel que joga aos princípios de direito internacional e o direito

Novel approaches to the characterization of metastasis in colorectal cancer

Comunicaciones a congreso

Label-free nanoplasmonic sensing of tumor-associate autoantibodies for early diagnosis of colorectal cancer

M. Soler acknowledges financial support from " Programa de Formación de Personal Investigador (FPI) " from the Spanish Ministry of Economy and Competitiveness (MINECO). R. Villar-Vazquez was a recipient of a FPU fellowship from the Spanish Ministry of Education and Culture.Colorectal cancer is treatable and curable when detected at early stages. However there is a lack of less invasive and more specific screening and diagnosis methods which would facilitate its prompt identification. Blood circulating autoantibodies which are immediately produced by the immune system at tumor appearance have become valuable biomarkers for preclinical diagnosis of cancer. In this work, we present the rapid and label-free detection of colorectal cancer autoantibodies directly in blood serum or plasma using a recently developed nanoplasmonic biosensor. Our nanoplasmonic device offers sensitive and real-time quantification of autoantibodies with excellent selectivity and reproducibility, achieving limits of detection around 1 nM (150-160 ng mL⁻¹). A preliminary evaluation of clinical samples of colorectal cancer patients has shown good correlation with ELISA. These results demonstrate the reliability of the nanobiosensor strategy and pave the way towards the achievement of a sensitive diagnostic tool for early detection of colorectal cancer

Intake, Energy Expenditure and Methane Emissions of Grazing Dairy Cows at Two Pre-Grazing Herbage Masses

A grazing experiment was undertaken to assess the effects of two levels of herbage mass (HM) on herbage DM intake (DMI), fat and protein corrected milk yield (FPCM), grazing behaviour, energy expenditure (HP), and methane emissions (CH4) of grazing dairy cows in spring. Treatments were a low HM (1447 kg DM/ha; LHM) or a high HM (1859 kg DM/ha; HHM). Pasture was composed mainly of cocksfoot (Dactylis glomerata) and lucerne (Medicago sativa), offered at a daily herbage allowance of 30 kg DM/cow, above 5 cm. Eight multiparous Holstein cows were used in a 2 × 2 Latin Square design in two 10-day periods. Despite the differences in pre-grazing HM between treatments, OM digestibility was not different (P = 0.28). Herbage mass did not affect DMI or FPCM. Grazing time was not different between treatments, but cows had a greater bite rate when grazing on LHM swards. However, HP did not differ between treatments. Daily methane emission (per cow), methane emission intensity (per kg FPCM) and methane yield (as percentage of gross energy intake) were not different. The lack of effect of the amount of pre-grazing HM on energy intake, confirms that the difference between HM treatments was beyond the limits that impose extra energy expenditure during grazing.Fil: Loza, Cecilia. Universidad de la República; UruguayFil: Gere, José Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Buenos Aires. Unidad de Investigación y Desarrollo de las Ingenierías; ArgentinaFil: Orcasberro, María Soledad. Universidad de la República; UruguayFil: Casal, Alberto. Universidad de la República; UruguayFil: Carriquiry, Mariana. Universidad de la República; UruguayFil: Juliarena, María Paula. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; ArgentinaFil: Ramírez-Bribiesca, Efren. Colegio de Postgraduados; MéxicoFil: Astigarraga, Laura. Universidad de la República; Urugua

Iodinated contrast agents for improving tumor imaging and quantification in rodents

2008 World Molecular Imaging Congress (WMIC 2008), 10-13 september 2008. Nice, Franc

Marine resources exploitation by hunter-gatherer and handfishing and tribal communities societies, in the historical region of the strait of Gibraltar

Desde la denominada Arqueología Social abordamos un análisis de la explotación de los recursos marinos por las formaciones sociales con modo de producción basado en la caza, recolección, pesca y marisqueo y por sociedades tribales comunitarias en la región histórica del Estrecho de Gibraltar. Su análisis nos acerca a los modos de vida y modos de trabajo de las sociedades desde el Pleistoceno Medio hasta el Holoceno Inicial en el sur de la Península Ibérica y Norte de África. Los avances de la investigación en los últimos años están arrojando resultados arqueomalacológicos de gran interés en esta área, evidenciándose la importancia de la pesca y el marisqueo como actividades básicas en el sustento alimenticio desde sociedades vinculadas a tecnología Modo III-Musteriense hasta los grupos tribales, cuando se definen verdaderos modos de vida de pesca y marisqueo.From so-called Social Archaeology boarded an analysis of the exploitation of marine resources for hunter-gatherer-shellfish and tribal community mode social formations in the historic region of the Gibraltar Strait. His analysis brings us closer to the lifestyles and ways of working of the societies from the Medium Pleistocene to the Early Holocene of the Iberian Peninsula and North Africa. Advances in recent year’s research are showing archaeo-malacological results of great interest in this area, demonstrating the importance of fishing and shelling such as activities of great importance in the alimentary sustenance from societies linked to a technology of Mode III- Mousterian, to tribal groups, when real lifestyles of fishing and shellfish are defining

Protumorigenic effects of Snail-expression fibroblasts on colon cancer cells

et al.Snail1 is a transcriptional factor that plays an important role in epithelial–mesenchymal transition and in the acquisition of invasive properties by epithelial cells. In colon tumors, Snail1 expression in the stroma correlates with lower specific survival of cancer patients. However, the role(s) of Snail1 expression in stroma and its association with patients' survival have not been determined. We used human primary carcinoma-associated fibroblasts (CAFs) or normal fibroblasts (NFs) and fibroblast cell lines to analyze the effects of Snail1 expression on the protumorigenic capabilities in colon cancer cells. Snail1 expression was higher in CAFs than in NFs and, as well as α-SMA, a classic marker of activated CAFs. Moreover, in tumor samples from 50 colon cancer patients, SNAI1 expression was associated with expression of other CAF markers, such as α-SMA and fibroblast activation protein. Interestingly, coculture of CAFs with colon cells induced a significant increase in epithelial cell migration and proliferation, which was associated with endogenous SNAI1 expression levels. Ectopic manipulation of Snail1 in fibroblasts demonstrated that Snail1 expression controlled migration as well as proliferation of cocultured colon cancer cells in a paracrine manner. Furthermore, expression of Snail1 in fibroblasts was required for the coadjuvant effect of these cells on colon cancer cell growth and invasion when coxenografted in nude mice. Finally, cytokine profile changes, particularly MCP-3 expression, in fibroblasts are put forward as mediators of Snail1-derived effects on colon tumor cell migration. In summary, these studies demonstrate that Snail1 is necessary for the protumorigenic effects of fibroblasts on colon cancer cells.This research was supported by the PI12/02037, Fundación Científica AECC, SAF2010-20750, S2010/BMD-2344, RTICC-RD12/0036/0041 and by the Fundación Banco Santander. Antonio García de Herreros’ laboratory was supported by RTICC-RD12/0036/0005 and SAF 2010-16089. Ma Jesús Larriba’s laboratory was supported by RD12/0036/0021. Cristina Peña and José Miguel García are recipients of Miguel Servet Contracts from the Instituto de Salud Carlos III.Peer reviewe

Spatio-temporal trends of Iberian wild boar contact with Mycobacterium tuberculosis complex detected by ELISA

The continuing expansion of Eurasian wild boar (Sus scrofa) populations raises concerns regarding disease transmission. In south-central Spain, overabundant wild boar are reservoirs of Mycobacterium bovis, and related members of the Mycobacterium tuberculosis complex (MTBC), the causative agents of bovine tuberculosis (bTB). An indirect enzyme-linked immunosorbent assay using bovine-purified protein derivative was applied to determine the spatial and temporal distribution of wild boar contact with MTBC in the Iberian Peninsula and to model and identify the associated risk factors. Wild boar apparent seroprevalence was 22%. Seropositives were detected in 71% of 81 sites, including 23 sites where wildlife was thought to be bTB free. The results described a new geographic range of wild boar contact with MTBC and a stable prevalence in this wildlife reservoir that contrasts with the success of bTB control in cattle. Inference of which host (wild boar or cattle) is driving bTB maintenance was not possible with our correlational results. The possibility of a wild boar bTB emergence in non-endemic regions should urgently be taken into account to avoid a future scenario resembling the current situation in south-central Spain.The current study is a contribution to JCCM PPIC10-0226-0243, MCINN Plan Nacional I+D+i AGL2008-03875, FAU2008-00004-C03 INIA and FEDER and to FP7 EU TB-STEP (212414). Studies on diseases shared between domestic animals and wildlife are also supported by Santander and Fundación Marcelino Botín, SDG Recursos Agrarios, Consejería de Medio Ambiente y Ordenación del Territorio de la Comunidad de Madrid, JCCM, Principado de Asturias, Gobierno de Aragón. PA is currently holding a Juan de la Cierva research contract awarded by the Ministerio de Ciencia e Innovación—Fondo Social Europeo.Peer Reviewe

A window onto the Eocene (Cenozoic): The palaeontological record of the Sobrarbe-Pirineos UNESCO Global Geopark (Huesca, Aragon, Spain)

The Sobrarbe-Pirineos UNESCO Global Geopark, located in the Central Pyrenees, is a region of remarkable geodiversity that includes extensive Eocene fossil-bearing sites and constitutes an important archive of paleobiodiversity. The Sobrarbe-Pirineos Geopark hosts outcrops of Eocene formations bearing an unusual abundance and diversity of fossils from marine and continental sedimentary environments, making the Sobrarbe-Pirineos Geopark a perfect window for learning about tropical ecosystems of the Eocene of southern Europe. These environments were in part tectonically controlled and offer a unique opportunity to understand how faunas changed in an active area. Here, we outline the main groups of fossils from the Sobrarbe-Pirineos Geopark, including popular examples such as the “Crocodile of Ordesa-Vio” and the sirenian Sobrarbesiren. The Geopark has been a major tool in the geoconservation of Eocene fossils