Um Caso de Urticária Multiforme em Criança

Urticaria multiforme is an acute hypersensitivity reaction which can be considered a morphological subtype of urticaria. It is more common in children and as its clinical presentation resembles other pathologies such as erythema multiforme, urticarial vasculitis and serum-sickness-like reaction, it has been often underdiagnosed. We report a case of a 1-year and 9-month-old child with exuberant skin lesions associated with systemic manifestations with complete and quick recovery after H1-antihistamines and iv steroids in an intensive care unit.A urticária multiforme é uma reação de hipersensibilidade aguda, sendo um subtipo morfológico de urticária. É mais comum em crianças e, uma vez que sua apresentação clínica se assemelha a outras dermatoses, como o eritema multiforme, a vasculite urticariana e a doença do soro-like, tem sido subdiagnosticada. Relatamos assim, um caso de uma criança de 1 ano e 9 meses com lesões cutâneas exuberantes associado a manifestações sistêmicas, com recuperação completa do quadro após tratamento com anti-histamínicos e corticosteróides endovenoso em unidade de medicina intensiva

Promoção do crescimento vegetal e diversidade genética de bactérias isoladas de nódulos de feijão‑caupi

The objective of this work was to evaluate the potential for plant growth promotion and the genetic diversity of bacteria isolated from nodules of cowpea grown in Cerrado soils in the state of Piauí, Brazil. Twenty‑six strains were evaluated as to their ability to fixate free‑living nitrogen, solubilize inorganic phosphates, produce indole‑3‑acetic acid (IAA) in the absence and presence of tryptophan (100 mg L-1), produce nodules, and promote cowpea growth in Leonard jars. No strain was able to fixate free‑living nitrogen, and 69% were able to solubilize calcium phosphate in vitro. In the presence of tryptophan, all strains were able to synthesize IAA in the 79 medium, and 80% synthesized IAA in the DYGS medium. Only four strains nodulated cowpea. The sequencing of the 16S rRNA gene identified the nodulating strains as belonging to the genera Bradyrhizobium, Rhizobium, Bacillus, and Paenibacillus. Among the non‑nodulating strains able to promote cowpea growth are the genera Bacillus and Paenibacillus.O objetivo deste trabalho foi avaliar o potencial de promoção do crescimento vegetal e a diversidade genética de bactérias isoladas de nódulos de feijão‑caupi cultivado em solos do Cerrado piauiense. Avaliaram-se 26 estirpes quanto à capacidade de fixar nitrogênio em vida livre, solubilizar fosfatos inorgânicos, produzir ácido‑3‑indolacético (AIA) na ausência e na presença do aminoácido triptofano (100 mg L-1), produzir nódulos e promover o crescimento de feijão‑caupi em vasos Leonard. Nenhuma estirpe fixou nitrogênio em vida livre, e 69% foram capazes de solubilizar fosfato de cálcio in vitro. Na presença de triptofano, todas as estirpes foram capazes de sintetizar o AIA em meio 79, e 80% sintetizaram o AIA em meio DYGS. Apenas quatro estirpes nodularam o feijão‑caupi. O sequenciamento do gene 16S rRNA identificou as estirpes nodulíferas como pertencentes aos gêneros Bradyrhizobium, Rhizobium, Bacillus e Paenibacillus. Entre as estirpes não nodulíferas promotoras do crescimento do feijão‑caupi, estão os gêneros Bacillus e Paenibacillus

Bactérias diazotróficas associativas: diversidade, ecologia e potencial de aplicações

AbstractBiological nitrogen fixation (BNF) is carried out by representatives of various bacterial phylogeneticgroups named diazotrophs. These bacteria can live free in many ecosystems and/or establishingsymbiosis or associations with diverse plant species. These last ones are called associative diazotrophs.Associative diazotrophic bacteria can contribute to plant growth by improving N-nutrition as wellas by other processes like hormone production, phosphate solubilization, biological control, amongothers. This review presents different genera of associative diazothophs regarding their taxonomicposition, ecological features and process they mediated related to plant growth promotion. It alsoshows how modifications in ecosystems influence density and diversity of these bacteria.A fixação biológica de nitrogênio (FBN) é realizada por representantes de diversos gruposfilogenéticos bacterianos, que são denominados diazotróficos. Estas bactérias podem viver livresem diversos ecossistemas, estabelecer simbioses ou estar associados às plantas, sendo neste últimocaso denominados de diazotróficas associativas. As bactérias diazotróficas associativas podemcontribuir para o crescimento vegetal não só pelo fornecimento de nitrogênio, mas também poroutros mecanismos como produção de fitormônios, solubilização de fosfatos, antagonismo afitopatógeno, entre outros. Esta revisão aborda os diferentes gêneros de diazotróficos associativose discute aspectos relacionados à taxonomia, ecologia e processos de promoção do crescimentovegetal e ainda como as alterações dos ecossistemas atuam na diversidade e densidade destasbactérias. Diazotrophic associative bacteria: diversity, ecology and potential applicationsAbstractBiological nitrogen fixation (BNF) is carried out by representatives of various bacterial phylogeneticgroups named diazotrophs. These bacteria can live free in many ecosystems and/or establishingsymbiosis or associations with diverse plant species. These last ones are called associative diazotrophs.Associative diazotrophic bacteria can contribute to plant growth by improving N-nutrition as wellas by other processes like hormone production, phosphate solubilization, biological control, amongothers. This review presents different genera of associative diazothophs regarding their taxonomicposition, ecological features and process they mediated related to plant growth promotion. It alsoshows how modifications in ecosystems influence density and diversity of these bacteria

Biazotrophic associative bacteria: diversity, ecology and potential applications

Biological nitrogen fixation (BNF) is carried out by representatives of various bacterial phylogeneticgroups named diazotrophs. These bacteria can live free in many ecosystems and/or establishingsymbiosis or associations with diverse plant species. These last ones are called associative diazotrophs.Associative diazotrophic bacteria can contribute to plant growth by improving N-nutrition as wellas by other processes like hormone production, phosphate solubilization, biological control, amongothers. This review presents different genera of associative diazothophs regarding their taxonomicposition, ecological features and process they mediated related to plant growth promotion. It alsoshows how modifications in ecosystems influence density and diversity of these bacteria

Bacterial strains from floodplain soils perform different plant-growth promoting processes and enhance cowpea growth

Certain nodulating nitrogen-fixing bacteria in legumes and other nodule endophytes perform different plant-growth promoting processes. The objective of this study was to evaluate 26 bacterial strains isolated from cowpea nodules grown in floodplain soils in the Brazilian savannas, regarding performance of plant-growth promoting processes and ability to enhance cowpea growth. We also identified these strains by 16S rRNA sequencing. The following processes were evaluated: free-living biological nitrogen fixation (BNF), solubilization of calcium, aluminum and iron phosphates and production of indole-3-acetic acid (IAA). The abilities to nodulate and promote cowpea growth were evaluated in Leonard jars. Partial sequencing of the 16S rRNA gene identified 60 % of the strains as belonging to genus Paenibacillus. The following four genera were also identified: Bacillus, Bradyrhizobium, Enterobacter and Pseudomonas. None of the strains fixed N2 free-living. Among the strains, 80 % solubilized Ca phosphate and one solubilized Al phosphate and none solubilized Fe phosphate. The highest IAA concentrations (52.37, 51.52 and 51.00 μg mL−1) were obtained in the 79 medium with tryptophan by Enterobacter strains UFPI B5-7A, UFPI B5-4 and UFPI B5-6, respectively. Only eight strains nodulated cowpea, however, all increased production of total dry matter. The fact that the strains evaluated perform different biological processes to promote plant growth indicates that these strains have potential use in agricultural crops to increase production and environmental sustainability