Polyhydroxyalkanoates Production by Mixed Microbial Culture under High Salinity

PTDC/BTA-BTA/30902/2017 UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020The fishing industry produces vast amounts of saline organic side streams that require adequate treatment and disposal. The bioconversion of saline resources into value-added products, such as biodegradable polyhydroxyalkanoates (PHAs), has not yet been fully explored. This study investigated PHA production by mixed microbial cultures under 30 gNaCl/L, the highest NaCl concentration reported for the acclimatization of a PHA-accumulating mixed microbial culture (MMC). The operational conditions used during the culture-selection stage resulted in an enriched PHA-accumulating culture dominated by the Rhodobacteraceae family (95.2%) and capable of storing PHAs up to 84.1% wt. (volatile suspended solids (VSS) basis) for the highest organic loading rate (OLR) applied (120 Cmmol/(L.d)). This culture presented a higher preference for the consumption of valeric acid (0.23 ± 0.03 CmolHVal/(CmolX.h)), and the 3HV monomer polymerization (0.33 ± 0.04 CmmolHV/(CmmolX.h) was higher as well. As result, a P(3HB-co-3HV)) with high HV content (63% wt.) was produced in the accumulation tests conducted at higher OLRs and with 30 gNaCl/L. A global volumetric PHA productivity of 0.77 gPHA/(L.h) and a specific PHA productivity of 0.21 gPHA/(gX.h) were achieved. These results suggested the significant potential of the bioconversion of saline resources into value-added products, such as PHAs.publishersversionpublishe

Traumatic neuroma of the mandible: a case report with spontaneous remission

Traumatic neuroma is a well-known disorder involving peripheral nerves, which occurs following trauma or sur - gery. The lesion develops most commonly in the soft tissues of the mental foramen area, lower lip and tongue. Intra- osseous lesions arising in jawbones are very uncommon. In this paper, we report a new case of an intra-osseous traumatic neuroma, discovered incidentally on a panoramic radiograph obtained for orthodontic documentation. In addition, the case herein described developed spontaneous remission, a situation not previously reported in the literature. Finally, we discuss relevant demographic, clinical, microscopic, immunohistochemical and treatment aspects of traumatic neuromas

Adesão à Terapêutica Alimentar Prescrita em Diabéticos Tipo1

[resumo][abstract

Comparing Soil Compaction under Different Grazing Systems with a Virgin Forest Soil to Determine Optimal Stocking Rates

The understanding of how soil physical properties respond to differing grazing practices may help explain the main causes of pasture degradation. Soil compaction has been shown to be a main degradation form of soil and the knowledge of techniques to quantify and rectify this are necessary to maintain optimal yields. This research aims to measure the rupture lines of red yellow latossol under differing pasture grazing practices compared to cropping and a natural forest. With this information it is aimed to calculate the correction factor for stocking rates and traffic of tillage tools. The differing soil management practices examined was, pasture grazed by sheep, and dairy cattle, a maize crop in no tillage cover-crop system and a natural forest. To quantify the soil physical changes, the direct shear test was used, which calculated the resultant force of a load. The resultant forces of the natural forest were compared against pasture systems and crop system, and a correction factor for stocking rates was calculated. The samples of Red yellow Latossol were equilibrated in the matrix potential (ψ): -6 kPa. In the shear test, the normal stress used was the 450kPa. The correction factor (CF) indicates whether the soil has structural degradation compared to natural forest. Values less than 1 indicated soil degradation. The pastures grazed by sheep and dairy cattle had values observed to be less than 1, excessive loads at high soil moisture may be attributed to this soil structural deformation. For these systems, grazing management and stocking rates should be corrected. The correction factor gives an indication of the magnitude of management change that is required (i.e. the stocking rate decreased). The crop area was found to have no soil strength issues, using the stress test

Improving processes in a postgraduate office of a university through lean office tools

This paper reports the administrative processes improvement based on Lean Office tools in an office environment of a University department. Lean tools such as electronic standardization were applied on the six educational projects of the department. Also, some were used to organize the computer desktop and network drives to improve the supporting educational projects information. Additionally, due to the absence of Key Performance Indicators (KPI), some here defined and implemented on the department. The main results were the electronic standardization successful implementation, reorganization of the drives and the desktop. The impacts were a reduction of 84% on the files search time, improvements on the development of forms for student I registration and projects managing, a 69% reduction of time searching for student information or data, a reduction of the input times and information handling in an estimated total of 12 hours/year, identification of KPI and development of a dashboard for visual analysis and monitoring of these.The authors want to acknowledge the LeanOffice@DPS team to support this work and the Department of Production and System-University of Minho where it was developed. The authors also would like to express their acknowledgments to national funds by COMPETE: POCI-01-0145-FEDER-007043 and FCT - Fundação para a Ciencia e Tecnologia within the Project Scope: UID/CEC/00319/2013

Short-term movements and habitat preferences of sailfish, Istiophorus platypterus (Istiophoridae), along the southeast coast of Brazil

Pop-up satellite archival tags (PSATs) were deployed on four sailfish, Istiophorus platypterus, in the coastal waters of Rio de Janeiro State in southeast Brazil during January and February of 2009 (sailfish I and II) and between November 2010 and January 2011 (sailfish III and IV). The total number of days monitored (i.e., time that the tags remained attached) were 12 (sailfish I), 51 (sailfish II), 16 (sailfish III) and 43 days (sailfish IV). The results indicate a clear pattern of vertical habitat utilization with the majority of the time spent concentrated near the uniform sea surface layer occupying a relatively narrow temperature range. Despite the clear preference for epipelagic surface waters, sailfish regularly undertook vertical excursions into deeper waters (>50 m) within three to six hour intervals. Most Probable Tracks (estimated from raw geolocations using the state-space Kalman filter model) and linear displacements suggested that tagged sailfish did not move significant distances from the tagging site. In brief, our report provides information regarding the biology of sailfish in the southwestern Atlantic and how vertical distributions during the day and night are influenced by water temperature and how this information can improve sailfish stock assessments in southwestern Atlantic Ocean.Quatro exemplares de agulhão-vela foram marcados com marcas eletrônicas monitoradas por satélite ('Pop-up satellite archival tags - PSATs') nas águas costeiras do Rio de Janeiro, sudeste do Brasil, durante janeiro e fevereiro de 2009 (agulhão-vela I e II) e entre novembro de 2010 e janeiro de 2011 (agulhão-vela III e IV). O número total de dias monitorados (ou seja, o tempo que as marcas permaneceram implantadas nos peixes) foram 12 (agulhão-vela I), 51 (agulhão-vela II), 16 (agulhão-vela III) e 43 dias (agulhão-vela IV). Os resultados demonstram um padrão claro de utilização do hábitat com a maior parte do tempo despendido predominantemente próximo à superfície do mar ocupando águas com uma faixa de temperatura restrita. Apesar da preferência por águas superficiais, os agulhões frequentemente realizaram mergulhos para águas mais profundas (ca. > 50 m) em intervalos de três a seis horas. A rota mais provável estimada a partir dos dados brutos de geolocalização e o modelo 'State-Space Kalman Filter' sugerem que os agulhões marcados não realizaram migrações significativas a partir do local de marcação. Em resumo, nossos resultados apresentam informações sobre a biologia da espécie no Atlântico Sudoeste e como as migrações verticais durante o dia e a noite são influenciadas pela temperatura da água e como essa informação pode auxiliar as avaliações de estoques de agulhão-vela no sudoeste do Atlântico.Universidade Federal de São Paulo (UNIFESP) Departamento de Ciências do MarUniversity of Florida School of Forest Resources and ConservationPelagic Research GroupInstituto de PescaUniversidade Federal Rural de Pernambuco Departamento de Pesca e AquiculturaUNIFESP, Depto. de Ciências do MarSciEL