New insights into functional regulation in MS-based drug profiling

We present a novel data analysis strategy which combined with subcellular fractionation and liquid chromatography-mass spectrometry (LC-MS) based proteomics provides a simple and effective workflow for global drug profiling. Five subcellular fractions were obtained by differential centrifugation followed by high resolution LC-MS and complete functional regulation analysis. The methodology combines functional regulation and enrichment analysis into a single visual summary. The workflow enables improved insight into perturbations caused by drugs. We provide a statistical argument to demonstrate that even crude subcellular fractions leads to improved functional characterization. We demonstrate this data analysis strategy on data obtained in a MS-based global drug profiling study. However, this strategy can also be performed on other types of large scale biological data.We thank Dr. Fridtjof Lund-Johansen for critical comments on the manuscript text. The Proteomics Resource Center at The Rockefeller University acknowledges funding from the Leona M. and Harry B. Helmsley Charitable Trust for mass spectrometer instrumentation. Cost of all experiments including MS analysis were supported by the Portuguese Foundation for Science and Technology (EXPL/DTP-PIC/0616/2013). R.M. is supported FCT investigator program 2012. A.S.C. is supported by the Portuguese Foundation for Science and Technology (FCT), financed by the European Social Funds (COMPETE-FEDER) and national funds of the Portuguese Ministry of Education and Science (POPH-QREN) fellowship SFRH/85569/2012

Removal of polycyclic aromatic hydrocarbons by biosorbents

Introduction: - Polycyclic aromatic hydrocarbons (PAHs) are a group of environmental carcinogens. They are formed during the incomplete combustion of organic matter. Humans are exposed to PAHs by various sources, including occupational environments, cigarette smoke, vehicle exhaust, and dietary sources as grilled and flame-broiled food. - In vivo studies in animals proved that PAHs are associated to cancer, and epidemiologic studies with exposed workers, especially in coke ovens and aluminium smelters, have shown clear excess of lung cancer and highly suggestive excesses of bladder cancer. - These compounds can enter in drinking water sources by precipitation and runoff on the earth’s surface. - Portuguese legislation for water for human consumption (DL 306/2007) proposes the determination of five PAHs; limits of the maximum concentration are 0.10 µg/L for total BghiP, BbF, BkF, IcdP, and 0.010 µg/L for BaP.This work was financial suported by the COMPETE program, under the Watercork project (nº. 2009/552).N/

Concepção e planeamento de armazéns

Uma cadeia de abastecimento pode ser considerada como uma rede de entidades individuais que, colectivamente são responsáveis pela gestão de fluxos de materiais e de informação desde os fornecedores até ao consumidor final. Assim, a eficiência e eficácia destas redes é fortemente condicionada pelas operações em cada uma das entidades que a integram. Neste sentido, os armazéns proporcionam uma importante ligação entre fornecedores, distribuidores e consumidores pelo que podem ser considerados uma entidade essencial na gestão da cadeia de abastecimento. A concepção e planeamento de um armazém envolve decisões complexas para as quais são diminutas ferramentas de apoio às decisões envolvidas. A complexidade das decisões envolvidas inclui: enorme quantidade de informação a ser processada; elevado número de possíveis alternativas; a existência de vários objectivos que por vezes são conflituosos bem como a incerteza associada ao fluxo de materiais dentro e fora do armazém. São diminutos os modelos de apoio à decisão que integrem várias decisões envolvidas na concepção e planeamento de armazéns. Esta escassez de modelos integradores que traduzam a complexidade destes sistemas é devida à dificuldade de análise e ao complexo tratamento analítico que lhes é inerente. Nesta comunicação será discutido um modelo que integra algumas das decisões envolvidas na concepção e planeamento de armazéns tais como: gestão de inventários, a atribuição de produtos a diferentes zonas de armazenagem e a alocação dos produtos dentro das áreas de armazenamento. O objectivo é mostrar as potencialidades e as fraquezas do modelo quando aplicado a uma variedade de problemas bem como identificar novas oportunidades de investigação

An optimisation model for the warehouse design and planning problem

In spite of the importance of warehouses in the field of the supply chain management, there is not a single decision model that integrates all the decisions that concerns the warehouse design and planning problem. In this paper we discuss a mathematical programming model aiming to support some warehouse management and inventory decisions. Our aim is to address the complexity related to the modeling of the warehouse design and planning problem. In particular an optimisation model is presented to capture the trade-offs among both inventory and warehouse costs in order to achieve global optimal design satisfying throughput requirements

An optimisation model for the warehouse design and product assignment and allocation problem

Warehouse design and planning is a great challenge in the field of Supply Chain Management. In this paper we discuss an optimisation model aiming to support some warehouse management decisions. In particular a mixed-integer programming model (MILP) is presented to determine product assignment and allocation to the functional areas, as well as the size of each area. Our aim is to capture the trade-offs among the different warehouse costs in order to achieve global optimal design satisfying throughput requirements

Integrated approaches to warehouse planning and operations

In this talk we discuss a tactical model recently available in warehouse literature. The model integrates the replenishment decision in inventory management, the allocation of products to warehousing systems and the assignment of products to storage locations in warehousing management. Our aim is to show the models’ potentialities and weaknesses when applied to a wide variety of problems and to identify challenging research opportunities for developing global warehouse decision support models that fill the gap between researchers and warehouse practitioners

Prefilter bandwidth effects in data symbol phase synchronizers of open loop

This work studies the effects of the prefilter bandwidth on the open loop symbol synchronizers. We consider three different prefilter bandwidth, namely, B1=¥ ( infinite), B2=2.tx and B3=1.tx, where tx is the transmission rate. We consider also four open loop symbol synchronizers, namely, the tank (tank), the SAW (SAW), the monostable (mon), and the astable (ast). The objective is to study the prefilter bandwidth with the four open loop symbol synchronizers and to evaluate their output jitter UIRMS (Unit Interval Root Mean Square) versus input SNR (Signal to Noise Ratio)

Prefilter bandwidth effects in sequential symbol synchronizers based on clock sampling by positive transitions

This work studies the effects of the prefilter bandwidth in the sequential symbol synchronizers based on clock sampling by positive transitions. The prefilter bandwidth B is switched between three values, namely B1=∞, B2=2. tx and B3=1. tx, where tx is the bit rate. The synchronizer has two variants, one discrete and other continuous. Each variant has two versions, one manual and other automatic. The objective is to study the prefilter bandwidth with the four synchronizers and to evaluate their output jitter UIRMS (Unit Interval Root Mean Square) versus input SNR (Signal to Noise Ratio)

Sequential symbol synchronizers based on clock sampling by positive transitions

This work presents a sequential symbol synchronizer, that was discovered by us, and its functioning principle is based on the clock sampling by the input positive data transitions. This synchronizer has two topologies, namely the discrete and the continuous. Also, each topology has two versions which are the manual and the automatic. These synchronizers are very interesting, because the previous adjust of the manual version isn’t critical. The objective is to study the four synchronizers and to evaluate their output jitter UIRMS (Unit Interval Root Mean Square) versus input SNR (Signal to Noise Ratio)