Perceived Damage by Elk in the Arkansas Ozarks

Wildlife managers in Arkansas are faced with managing a growing population of Rocky Mountain elk (Cervus elaphus neloni) that has extended its range to incorporate private lands near the Buffalo National River (BNR) in northcentral Arkansas. This range expansion has created conflicts between private landowners and wildlife management personnel. To document the extent of damage and assess attitudes of landowners with elk on their land, interviews were conducted with landowners who contacted us or the Arkansas Game and Fish Commission about problems with elk. A survey also was created and sent to landowners who live near the BNR in Boone and Newton counties and who may have elk on their land. Ten of 18 respondents with elk on their land reported having a problem with nuisance activity. Landowners indicated that most damage was to pastures, hay crops, and food plots. Damage appeared to occur more often in summer, when elk home ranges were smallest, than in other seasons. Landowners incurring damage from elk had a strong negative opinion. Continued research into effective management practices should be conducted to properly manage this growing population of elk and reduce conflicts between elk and Arkansas landowners

The Phylum Cnidaria: A Review of Phylogenetic Patterns and Diversity 300 Years After Linnaeus

Systema Naturae includes representatives of every major lineage of the animal phylum Cnidaria. However, Linnaeus did not classify the members of the phylum as is now done, and the diversity of the group is not well represented. We contrast the Linnaean perspective on cnidarian diversity with the modern, phylogenetic perspective. For each order, we detail diversity at the family level, providing phylogenetic context where possible

Complex -Glycans Influence the Spatial Arrangement of Voltage Gated Potassium Channels in Membranes of Neuronal-Derived Cells

The intrinsic electrical properties of a neuron depend on expression of voltage gated potassium (Kv) channel isoforms, as well as their distribution and density in the plasma membrane. Recently, we showed that N-glycosylation site occupancy of Kv3.1b modulated its placement in the cell body and neurites of a neuronal-derived cell line, B35 neuroblastoma cells. To extrapolate this mechanism to other N-glycosylated Kv channels, we evaluated the impact of N-glycosylation occupancy of Kv3.1a and Kv1.1 channels. Western blots revealed that wild type Kv3.1a and Kv1.1 α-subunits had complex and oligomannose N-glycans, respectively, and that abolishment of the N-glycosylation site(s) generated Kv proteins without N-glycans. Total internal reflection fluorescence microscopy images revealed that N-glycans of Kv3.1a contributed to its placement in the cell membrane while N-glycans had no effect on the distribution of Kv1.1. Based on particle analysis of EGFP-Kv proteins in the adhered membrane, glycosylated forms of Kv3.1a, Kv1.1, and Kv3.1b had differences in the number, size or density of Kv protein clusters in the cell membrane of neurites and cell body of B35 cells. Differences were also observed between the unglycosylated forms of the Kv proteins. Cell dissociation assays revealed that cell-cell adhesion was increased by the presence of complex N-glycans of Kv3.1a, like Kv3.1b, whereas cell adhesion was similar in the oligomannose and unglycosylated Kv1.1 subunit containing B35 cells. Our findings provide direct evidence that N-glycans of Kv3.1 splice variants contribute to the placement of these glycoproteins in the plasma membrane of neuronal-derived cells while those of Kv1.1 were absent. Further when the cell membrane distribution of the Kv channel was modified by N-glycans then the cell-cell adhesion properties were altered. Our study demonstrates that N-glycosylation of Kv3.1a, like Kv3.1b, provides a mechanism for the distribution of these proteins to the cell body and outgrowths and thereby can generate different voltage-dependent conductances in these membranes

Arms and the mollusc: An evolutionary arms race has produced armor based on molluscan biomineralization

A.G.C. acknowledges funding from Project No. PID2020116660GB-I00, funded by Spanish Ministry of Science and Innovation (MCIN/AEI/10.13039/ 501100011033). Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature.More than half a billion years ago in the early Cambrian period, there began an evolutionary arms race between molluscs and their predators, in which molluscs developed armor in the form of a biomineral exoskeleton—a shell—to avoid being eaten by predators that were developing jaws and other novel means of devouring them. The mollusc fabricates multiple layers of shell, each of a particular microstructure of a composite between an inorganic and an organic phase, which are the end result of more than 500 million years of coevolution with increasingly deadly predators. Molluscan biomineralization is an excellent case to study how a biological process produces a complex structure, because the shell is constructed as an extracellular structure in which all construction materials are passed out of the cells to self-assemble outside the cell wall. We consider what is known of the development of multilayer composite armor in the form of nacre (mother of pearl) and the other strong microstructures with which molluscs construct their shells.MCIN/AEI/10.13039/ 501100011033 PID2020116660GB-I00CRUE-CSI

Ultrasound in the Evaluation of Radial Neuropathies at the Elbow

There are five sites at which radial nerve entrapment at the elbow has been commonly reported. These include the level of the fibrous bands within the extensor carpi radialis brevis, the thickened fascial tissue at the radiocapitellar joint, the leash of Henry, the arcade of Frohse, and the distal border of the supinator muscle. This review describes the anatomy of the radial nerve at the elbow and the surrounding structures, and then provides an overview of the literature supporting the use of ultrasound to assist in the evaluation of suspected radial neuropathy at the elbow. This review concludes with a suggested ultrasonographic approach for the systematic evaluation of suspected radial neuropathy at the elbow

Ethylene supports colonization of plant roots by the mutualistic fungus Piriformospora indica

The mutualistic basidiomycete Piriformospora indica colonizes roots of mono- and dicotyledonous plants, and thereby improves plant health and yield. Given the capability of P. indica to colonize a broad range of hosts, it must be anticipated that the fungus has evolved efficient strategies to overcome plant immunity and to establish a proper environment for nutrient acquisition and reproduction. Global gene expression studies in barley identified various ethylene synthesis and signaling components that were differentially regulated in P. indica-colonized roots. Based on these findings we examined the impact of ethylene in the symbiotic association. The data presented here suggest that P. indica induces ethylene synthesis in barley and Arabidopsis roots during colonization. Moreover, impaired ethylene signaling resulted in reduced root colonization, Arabidopsis mutants exhibiting constitutive ethylene signaling, -synthesis or ethylene-related defense were hyper-susceptible to P. indica. Our data suggest that ethylene signaling is required for symbiotic root colonization by P. indica

Phylogenetics of Hydroidolina (Hydrozoa: Cnidaria)

This is the published version, also available here: http://dx.doi.org/10.1017/S0025315408002257.Hydroidolina is a group of hydrozoans that includes Anthoathecata, Leptothecata and Siphonophorae. Previous phylogenetic analyses show strong support for Hydroidolina monophyly, but the relationships between and within its subgroups remain uncertain. In an effort to further clarify hydroidolinan relationships, we performed phylogenetic analyses on 97 hydroidolinan taxa, using DNA sequences from partial mitochondrial 16S rDNA, nearly complete nuclear 18S rDNA and nearly complete nuclear 28S rDNA. Our findings are consistent with previous analyses that support monophyly of Siphonophorae and Leptothecata and do not support monophyly of Anthoathecata nor its component subgroups, Filifera and Capitata. Instead, within Anthoathecata, we find support for four separate filiferan clades and two separate capitate clades (Aplanulata and Capitata sensu stricto). Our data however, lack any substantive support for discerning relationships between these eight distinct hydroidolinan clades

Glucocorticoid excess alters metabolic rate and substrate utilisation via 11β-HSD1

Systemic glucocorticoid excess causes several adverse metabolic conditions, most notably Cushing’s syndrome. These effects are amplified by the intracellular enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1). Here we determined the less well characterised effects of glucocorticoid excess, and the contribution of 11β-HSD1 amplification, on metabolic rate in mice. Male and female C57BL/6J (wild type, WT) and 11β-HSD1 knock out (11β-HSD1KO) mice were treated with high-dose corticosterone or a vehicle control for 3 weeks. Indirect calorimetry was conducted during the final week of treatment, with or without fasting, to determine the impact on metabolic rate. We found that corticosterone treatment elevated metabolic rate and promoted carbohydrate utilisation primarily in female WT mice, with effects more pronounced during the light phase. Corticosterone treatment also resulted in greater fat accumulation in female WT mice. Corticosterone induced hyperphagia was identified as a likely causal factor altering the respiratory exchange ratio (RER) but not energy expenditure (EE). Male and female 11β-HSD1KO mice were protected against these effects. We identify novel metabolic consequences of sustained glucocorticoid excess, identify a key mechanism of hyperphagia and demonstrate that 11β-HSD1 is required to manifest the full metabolic derangement