Comparison of Packaging Strategy in Retroviruses and Pararetroviruses

AbstractReverse transcription is not solely a retroviral mechanism. Animal hepadnaviruses, plant caulimoviruses, and badnaviruses have a RNA intermediate which is reverse transcribed into double-stranded DNA genome. Based on this fact, these three groups of viruses have been regrouped under the name of pararetroviruses. Although each one has developed its own strategy to assure an efficient packaging of their genome, it is clear that they have adopted a strategy where encapsidation prepares for initiation of reverse transcription. This is discussed in this review

A New Marker on Chicken Hematopoietic Cells is Defined by a Monoclonal Antibody Raised Against a V ß Chain of the Human TCR

In this paper, we show that a mouse monoclonal antibody, 111-427, specific for the V ß 5.3 chain of the human T-cell receptor (TCR) for antigen, also reacts with chicken hematopoietic cells. Our data indicate that the majority of 111-427 positive cells among peripheral blood leucocytes (PBL) are thrombocytes. This antibody also recognizes two in vitro cell lines, III-C5, an IL-2-dependent T-cell-line and HD11, a macrophage cell line. In addition, erythrocytes and a minor subpopulation of thymus and spleen cells are also stained by the monoclonal antibody (mAb). No specific immunoprecipitation could be detected from 125I radiolabeled cell lysates. By Western blotting techniques, the 111- 427 mAb identifies a single band of apparent molecular weight 91 kD, unaffected by reduction, from III-C5 and HD11 cell lysates. This band is absent in negative cell control lysates. On thrombocytes, the apparent molecular weight of the band is shifted to 87 kD. These results indicate that the mAb does not recognize the chicken T-cell receptor for antigen, but a cell surface marker shared primarily between thrombocytes and erythrocytes. This new chicken cell marker is compared to other cell surface markers in avian or mammalian species that present some analogies in their tissue distribution

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Organisation génomique, phylogénie et taxonomie polyphasique des bactéries du genre Veillonella et des genres apparentés du sous-groupe Sporomusa

MONTPELLIER-BU Médecine UPM (341722108) / SudocMONTPELLIER-BU Médecine (341722104) / SudocPARIS-BIUP (751062107) / SudocSudocFranceF

Etude de la résistance aux fluoroquinolones chez Listeria monocytogenes

MONTPELLIER-BU Médecine UPM (341722108) / SudocPARIS-BIUM (751062103) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF

Détection moléculaire du génotype de résistance aux glycopeptides des entérocoques (intérêt de la Multiplex PCR)

MONTPELLIER-BU Médecine UPM (341722108) / SudocPARIS-BIUM (751062103) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF

Corrosion galvanique du magnesium avec le graphite en presence de chlorures

National audienc

A Script for Archiving Digital Research Data: Improving Accuracy and Efficiency in the Dataverse Network

My colleagues and I assessed the workflow for data ingest at the Odum Archive at the University of North Carolina. We then made recommendations based on our analysis of the system. This poster discusses the most important recommendation that we gave the staff of the archive. We suggested that they create a script which would automate the steps in the workflow that involve a single point of failure. With the script, the single point of failure remains, but the risk of error has been significantly lessened

Mise en place d’un dispositif de connaissance, suivi et évaluation socio-économique et environnemental de la Nouvelle Aire Protégée (NAP) du Makay, Madagascar

Covering an area of approximately 4,000 km2, the New Protected Area of Makay, Madagascar, formalized in2017, is emblematic of the richness of biodiversity, with an exceptional rate of endemism, and the natural andcultural heritage of Madagascar. This document presents the challenges and the methodological options forsetting up a socio-economic and environmental knowledge, monitoring and evaluation system. The deviceconsists of three articulated arms, combining social sciences and natural and earth sciences, both quantitativeand qualitative: a quantitative arm of socio-economic and environmental surveys among the populations ofMakay; an ecological monitoring arm; and a qualitative socio-anthropological arm. By putting the people wholive on the edge of the Makay at the heart of the device, it proposes to push the frontier of existinginformation systems, still underdeveloped in this field, despite their decisive importance on a global scale, inorder to better understand the links between conservation and development.D’une superficie d’environ 4 000 km2, la Nouvelle Aire Protégée du Makay, Madagascar, officialisée en 2017,est emblématique de la richesse de la biodiversité, avec un taux d’endémisme exceptionnel, et du patrimoinenaturel et culturel malgache. Le présent document expose les enjeux et les options méthodologiques pour lamise en place d’un dispositif de connaissance, suivi et évaluation socio-économique et environnemental. Ledispositif se compose de trois bras articulés, combinant sciences sociales et sciences de la nature et de la terre,aussi bien quantitatifs que qualitatifs : un dispositif quantitatif d’enquêtes socio-économiques etenvironnementales auprès des populations du Makay ; un dispositif de suivi écologique ; un dispositif qualitatifde type socio-anthropologique. En mettant les populations qui vivent aux pourtours du Makay au coeur dudispositif, il se propose de repousser la frontière des systèmes d’informations existants, à ce jour balbutiantsdans ce domaine, malgré leur importance décisive à l’échelle mondiale, afin de mieux comprendre les liensentre conservation et développement