Brassica rapa hairy root based expression system leads to the production of highly homogenous and reproducible profiles of recombinant human alpha-L-iduronidase

International audienceThe Brassica rapa hairy root based expression platform, a turnip hairy root based expression system, is able to produce human complex glycoproteins such as the alpha—L—iduronidase (IDUA) with an activity similar to the one produced by Chinese Hamster Ovary (CHO) cells. In this article, a particular attention has been paid to the N‐ and O‐glycosylation that characterize the alpha‐L‐iduronidase produced using this hairy root based system. This analysis showed that the recombinant protein is characterized by highly homogeneous post translational profiles enabling a strong batch to batch reproducibility. Indeed, on each of the 6 N‐glycosylation sites of the IDUA, a single N‐glycan composed of a core Man3GlcNAc2 carrying one beta(1,2)‐xylose and one alpha(1,3)‐fucose epitope (M3XFGN2) was identified, highlighting the high homogeneity of the production system. Hydroxylation of proline residues and arabinosylation were identified during O‐glycosylation analysis, still with a remarkable reproducibility. This platform is thus positioned as an effective and consistent expression system for the production of human complex therapeutic proteins

brainlife.io: A decentralized and open source cloud platform to support neuroscience research

Neuroscience research has expanded dramatically over the past 30 years by advancing standardization and tool development to support rigor and transparency. Consequently, the complexity of the data pipeline has also increased, hindering access to FAIR data analysis to portions of the worldwide research community. brainlife.io was developed to reduce these burdens and democratize modern neuroscience research across institutions and career levels. Using community software and hardware infrastructure, the platform provides open-source data standardization, management, visualization, and processing and simplifies the data pipeline. brainlife.io automatically tracks the provenance history of thousands of data objects, supporting simplicity, efficiency, and transparency in neuroscience research. Here brainlife.io's technology and data services are described and evaluated for validity, reliability, reproducibility, replicability, and scientific utility. Using data from 4 modalities and 3,200 participants, we demonstrate that brainlife.io's services produce outputs that adhere to best practices in modern neuroscience research

brainlife.io: a decentralized and open-source cloud platform to support neuroscience research

Neuroscience is advancing standardization and tool development to support rigor and transparency. Consequently, data pipeline complexity has increased, hindering FAIR (findable, accessible, interoperable and reusable) access. brainlife.io was developed to democratize neuroimaging research. The platform provides data standardization, management, visualization and processing and automatically tracks the provenance history of thousands of data objects. Here, brainlife.io is described and evaluated for validity, reliability, reproducibility, replicability and scientific utility using four data modalities and 3,200 participants

Alien Registration- Caron, Marie Aurore A. (Lewiston, Androscoggin County)

https://digitalmaine.com/alien_docs/22778/thumbnail.jp

Novel strategies of photopolymerisation and polymer’s modifications for application in 3D-4D printing

Le but de ce travail de thèse a été d’explorer de nouveaux concepts et des molécules innovantes pour développer différentes techniques de polymérisation ou différents matériaux visant l’impression 3D/4D comme application finale. Pour cela, trois principaux axes de recherche ont été définis. Tout d’abord, des recherches ont été menées pour le décalage des longueurs d’onde d’irradiation vers le proche-infrarouge (NIR), justifié principalement par une plus grande pénétration de la lumière dans les substrats. Pour surmonter la faible énergie apportée au système, le principe d’Upconversion par Annihilation Triplet-Triplet a notamment été étudié, avant de tirer parti des propriétés photothermiques (conversion de la lumière incidente en énergie thermique) de différentes molécules (e.g. cyanines ou complexes photo-sensibilisateurs) sous ce type d’irradiation. Dans un deuxième temps, des stratégies complémentaires ont été étudiées pour accéder à une polymérisation en profondeur (ou pour des échantillons présentant des zones d’ombres) : la polymérisation frontale photo-amorcée et la chimiluminescence. Enfin, les réseaux dynamiques basés sur des liaisons covalentes et supramoléculaires ont été étudiés au travers de deux molécules synthétisées par des laboratoires partenaires, l’une présentant des liaisons de type imine et hydrogène (avec l’eau et la température étudiés comme stimuli) et l’autre des liaisons de type azo photoisomérisables.The purpose of this PhD work was to investigate new concepts and molecules to develop different polymerization’s methods or different material which could be apply to 3D/4D printing. Three main research axes have been determined. First of all, experiments have been carried out to shift the irradiation wavelength towards near-infrared (NIR), justified by a deeper penetration in the subtract. To overcome the poor energy supplied to the system by those wavelengths, the Upconversion by Triplet-Triplet Annihilation principle has been studied, before taking advantage of the photothermal effect (conversion of incident light into thermal energy) of different molecules (e.g. cyanines or photosensitizer complexes) under this type of irradiation. In a second time, additional strategies have been studied to have access to thick sample (or sample with shadow zone): photoinduced frontal polymerization and chemiluminescence. Finally, dynamic network based on covalent and supramolecular bonds have been studied through two molecules synthetized by partner laboratory, one with imine and hydrogen bonds (with water and temperature as stimuli) and one with a photoizomerizable azo-type bond

Nouvelles stratégies de photopolymérisation et modifications des polymères pour des applications en impression 3D-4D

The purpose of this PhD work was to investigate new concepts and molecules to develop different polymerization’s methods or different material which could be apply to 3D/4D printing. Three main research axes have been determined. First of all, experiments have been carried out to shift the irradiation wavelength towards near-infrared (NIR), justified by a deeper penetration in the subtract. To overcome the poor energy supplied to the system by those wavelengths, the Upconversion by Triplet-Triplet Annihilation principle has been studied, before taking advantage of the photothermal effect (conversion of incident light into thermal energy) of different molecules (e.g. cyanines or photosensitizer complexes) under this type of irradiation. In a second time, additional strategies have been studied to have access to thick sample (or sample with shadow zone): photoinduced frontal polymerization and chemiluminescence. Finally, dynamic network based on covalent and supramolecular bonds have been studied through two molecules synthetized by partner laboratory, one with imine and hydrogen bonds (with water and temperature as stimuli) and one with a photoizomerizable azo-type bond.Le but de ce travail de thèse a été d’explorer de nouveaux concepts et des molécules innovantes pour développer différentes techniques de polymérisation ou différents matériaux visant l’impression 3D/4D comme application finale. Pour cela, trois principaux axes de recherche ont été définis. Tout d’abord, des recherches ont été menées pour le décalage des longueurs d’onde d’irradiation vers le proche-infrarouge (NIR), justifié principalement par une plus grande pénétration de la lumière dans les substrats. Pour surmonter la faible énergie apportée au système, le principe d’Upconversion par Annihilation Triplet-Triplet a notamment été étudié, avant de tirer parti des propriétés photothermiques (conversion de la lumière incidente en énergie thermique) de différentes molécules (e.g. cyanines ou complexes photo-sensibilisateurs) sous ce type d’irradiation. Dans un deuxième temps, des stratégies complémentaires ont été étudiées pour accéder à une polymérisation en profondeur (ou pour des échantillons présentant des zones d’ombres) : la polymérisation frontale photo-amorcée et la chimiluminescence. Enfin, les réseaux dynamiques basés sur des liaisons covalentes et supramoléculaires ont été étudiés au travers de deux molécules synthétisées par des laboratoires partenaires, l’une présentant des liaisons de type imine et hydrogène (avec l’eau et la température étudiés comme stimuli) et l’autre des liaisons de type azo photoisomérisables

Alien Registration- Caron, Marie Aurore A. (Lewiston, Androscoggin County)

https://digitalmaine.com/alien_docs/22778/thumbnail.jp

Near‐infrared ‐induced photothermal decomposition of charge transfer complexes: A new way to initiate thermal polymerization

International audienceA new approach for the Near-Infrared induced free radical photopolymerisation (FRP) of (meth)acrylate-based polymers through the use of Charge Transfer Complex (CTC) is proposed. This challenging strategy is based on Near-Infrared sensitizers (e.g. cyanines) which act as very efficient heat generator (heater) upon NIR irradiation, allowing the activation of CTC in-situ formed between an electron rich N-aromatic amine and an iodonium salt used as an electron acceptor. The thermal ability of the different systems to initiate a polymerization upon thermal activation has been investigated by Differential Scanning Calorimetry (DSC) and their behaviors upon NIR irradiation have been examined by thermal imaging experiments. The role of cyanines has also been highlighted by spectrophotometry as well as Fourier Transform Infrared (FTIR) spectrometry. Finally, to highlight the huge potential of the proposed approach, these systems have been tested on glass and carbon fibres prepregs to the composite's development

Near-Infrared PhotoInitiating Systems: Photothermal Effect vs. Triplet-Triplet Annihilation -based UpConversion Polymerization

International audienceNIR light induced polymerization has attracted more and more attention in the photopolymerization field due to the possibility to use safer and more penetrating wavelengths, reducing the hazardousness. Here, a novel perspective for the Free Radical Polymerization (FRP) of acrylate-based monomers based on Triplet-Triplet Annihilation UpConversion (TTA-UC) is proposed, avoiding the introduction of heavy metals, usually required in the TTA processes. Thermal imaging experiments and Real Time Fourier Transform Infrared (RT-FTIR) spectroscopy have been respectively used to record the temperature during NIR irradiation and measure the reactive function conversion. The competition between the TTA-UC and the NIR photothermal activation was investigated to compare the relative efficiency of both NIR processes. In view of the results obtained by the different methods, the photothermal effect seems to get the hupper hand over the photoactivation of the system

Le « dangereux » métier d’élu local : réflexions juridiques sur les risques du métier

National audienceCet article envisage la responsabilité pénale des élus locaux, en particulier en matière de commande publique