Plan de mejora en la gestión de seguridad y su impacto en la reducción de accidentes laborales en la empresa LCL Contratistas S.A.C., 2023

La investigación tuvo como objetivo general evaluar de qué manera el plan de mejora del sistema de gestión de seguridad influye en la reducción de los accidentes laborales de la empresa LCL CONTRATISTAS S.A.C., dentro de la empresa de estudio se halló varios problemas que afectaban a sus trabajadores y a la organización, pues no cumplían adecuadamente con el sistema de gestión de seguridad por ende frecuentemente reportaban accidentes laborales. Se inició realizando la búsqueda de antecedentes, investigaciones similares, definiciones sobre los temas relacionados. Con respecto a la metodología fue de tipo aplicado, diseño experimental, de enfoque cuantitativo y como instrumento se utilizó la observación y medición directa y análisis documental, todo esto para poder obtener los datos se validaron por medio de tres juicios de expertos. Finalmente, como resultados se obtuvo que la implementación del plan de mejora logró disminuir la cantidad de accidentes leves a un 26% y los accidentes incapacitantes a un 25%, de igual manera los índices de frecuencia y severidad redujeron, esto también favoreció a la disminución de la cantidad de días laborables perdidos; su propósito fue mejorar las actividades laborales y aumentar la productividad y rentabilidad de la empresa

Chromatin Dynamics in the Fission Yeast, Schizosaccharomyces pombe

In the eukaryotic cell nucleus, spatial organization and dynamics of the genome is important in the regulation of gene expression. This thesis describes the use of the fission yeast, Schizosaccharomyces pombe, to study chromatin regulation and dynamics. We used nitrogen starvation to induce transcription of genes in fission yeast cells. In induced genes, nucleosomes get evicted in both the promoter and in the open reading frame (ORF). In the genes with the highest expression more nucleosomes get evicted from the ORF than from the promoter. This indicates that large rearrangements of the chromatin are occurring during a drastic gene induction. Many of the genes that become expressed early after nitrogen starvation are located together in clusters. In a cell where nitrogen is present in the surrounding media the gene clusters locate close to the nuclear periphery. When the nitrogen source is removed from the media, the clusters move to a more internal position. Thus rearrangement of chromatin due to gene induction, described in the first study, is accompanied by subnuclear changes of localization. Another type of regulation is the silencing of genes. We have studied a factor necessary for correct repression of genes located in silent chromatin, in S. pombe. The protein, Clr2, is part of the SHREC complex containing a remodeler (Mit1) and a histone deacetylase (Clr3). By bioinformatic analysis of Clr2 and newly sequenced fungi genomes, three motifs were identified. To gather more information about important parts of the Clr2 protein, deletions were made. When removing from about 20 to 100 amino acids in the middle of the protein, silencing of a reporter gene inserted at the mating-type region, inner repeats of centromere 1 and at the central core of centromere 2, failed. This indicates that Clr2 has an important role in establishing silent chromatin

Chromatin Dynamics in the Fission Yeast, Schizosaccharomyces pombe

In the eukaryotic cell nucleus, spatial organization and dynamics of the genome is important in the regulation of gene expression. This thesis describes the use of the fission yeast, Schizosaccharomyces pombe, to study chromatin regulation and dynamics. We used nitrogen starvation to induce transcription of genes in fission yeast cells. In induced genes, nucleosomes get evicted in both the promoter and in the open reading frame (ORF). In the genes with the highest expression more nucleosomes get evicted from the ORF than from the promoter. This indicates that large rearrangements of the chromatin are occurring during a drastic gene induction. Many of the genes that become expressed early after nitrogen starvation are located together in clusters. In a cell where nitrogen is present in the surrounding media the gene clusters locate close to the nuclear periphery. When the nitrogen source is removed from the media, the clusters move to a more internal position. Thus rearrangement of chromatin due to gene induction, described in the first study, is accompanied by subnuclear changes of localization. Another type of regulation is the silencing of genes. We have studied a factor necessary for correct repression of genes located in silent chromatin, in S. pombe. The protein, Clr2, is part of the SHREC complex containing a remodeler (Mit1) and a histone deacetylase (Clr3). By bioinformatic analysis of Clr2 and newly sequenced fungi genomes, three motifs were identified. To gather more information about important parts of the Clr2 protein, deletions were made. When removing from about 20 to 100 amino acids in the middle of the protein, silencing of a reporter gene inserted at the mating-type region, inner repeats of centromere 1 and at the central core of centromere 2, failed. This indicates that Clr2 has an important role in establishing silent chromatin

Chromatin Dynamics in the Fission Yeast, Schizosaccharomyces pombe

In the eukaryotic cell nucleus, spatial organization and dynamics of the genome is important in the regulation of gene expression. This thesis describes the use of the fission yeast, Schizosaccharomyces pombe, to study chromatin regulation and dynamics. We used nitrogen starvation to induce transcription of genes in fission yeast cells. In induced genes, nucleosomes get evicted in both the promoter and in the open reading frame (ORF). In the genes with the highest expression more nucleosomes get evicted from the ORF than from the promoter. This indicates that large rearrangements of the chromatin are occurring during a drastic gene induction. Many of the genes that become expressed early after nitrogen starvation are located together in clusters. In a cell where nitrogen is present in the surrounding media the gene clusters locate close to the nuclear periphery. When the nitrogen source is removed from the media, the clusters move to a more internal position. Thus rearrangement of chromatin due to gene induction, described in the first study, is accompanied by subnuclear changes of localization. Another type of regulation is the silencing of genes. We have studied a factor necessary for correct repression of genes located in silent chromatin, in S. pombe. The protein, Clr2, is part of the SHREC complex containing a remodeler (Mit1) and a histone deacetylase (Clr3). By bioinformatic analysis of Clr2 and newly sequenced fungi genomes, three motifs were identified. To gather more information about important parts of the Clr2 protein, deletions were made. When removing from about 20 to 100 amino acids in the middle of the protein, silencing of a reporter gene inserted at the mating-type region, inner repeats of centromere 1 and at the central core of centromere 2, failed. This indicates that Clr2 has an important role in establishing silent chromatin

Proyecto de creación de estrategias para aumentar las negociaciones bursatiles en la bvg (bolsa de valores de Guayaquil)

El mercado ecuatoriano tiene un gran potencial para realizar inversiones que produzcan alta rentabilidad en diversos sectores económicos, y que corresponde a la BVG ser el vehículo que difunda una cultura bursátil en el entorno y trasmita el know-how del mercado de valores. El mercado de valores en general, canaliza los recursos financieros hacia las actividades productivas a través de las negociaciones de valores. Constituye una fuente directa de financiamiento y una opción de rentabilidad para los inversionistas

Evaluación del uso recreativo del club garza roja

En el siguiente estudio se presenta la “Evaluación del Uso Recreativo del Club Garza Roja”, para evaluar el correcto uso y funcionamiento del club. A continuación se presentan tres capítulos, en donde se detalla la información pertinente para el desarrollo del documento. En el primer capítulo, se menciona lo siguiente: • El Club Garza Roja es un centro de recreación turística ubicado en el cantón Nobol, provincia del Guayas, basado en la inspiración de su creador el Sr. Ramón Sonnenholzner • Los visitantes pueden acceder al club en vehículos particulares, o por medio de las cooperativas de buses Intercantonales como “Mi Piedacita”, “Señor de los Milagros”, “Rutas Vinceñas”, y otras • Para el entretenimiento de los visitantes el club cuenta con una variada planta turística, y por ende se pueden desarrollar las diferentes actividades que se ofrecen en el club. En el segundo capítulo, se menciona lo siguiente: • La encuesta se utilizó como herramienta de investigación, para determinar el nivel de satisfacción, y el perfil del visitante. Se realizaron 196 encuestas en las instalaciones del club, y en las ciudades de Guayaquil, Nobol, Daule considerando a personas que han frecuentado el club con anterioridad • La mayoría de los visitantes pertenecen al sexo femenino (57%), sus edades oscilan entre los 19 a 26 años (65%), provienen de la ciudad de Guayaquil (79%), son personas con estudios universitarios (65%), con ingresos mensuales menos de $200 (79%), asisten al club con 3 a 5 acompañantes (47%), con un promedio de visita entre 1 a 3 veces al año (84%), su principal motivación es la recreación (35%), y el medio de transporte más utilizado es el vehículo particular (61%) • Los visitantes en su mayoría (56%) están satisfechos con las instalaciones y con el servicio ofrecido por el club. El porcentaje restante no tienen el mismo criterio por encontrar las instalaciones en mal estado En el tercer capítulo, se menciona lo siguiente: Al aplicar las fórmulas de capacidad de carga se obtuvo la cantidad de personas que pueden visitar el Club Garza Roja, usar las instalaciones y áreas recreativas con espacios cómodos • Al aplicar la Matriz de Leopold Modificada, se obtuvieron los impactos ambientales y sociales positivos y negativos del Club Garza Roja. A cada acción negativa, se le ha creado una actividad de mitigació

Evaluación del uso recreativo del club garza roja

El presente estudio tiene como objetivo principal evaluar el uso recreativo del club Garza Roja y conocer el carácter cultural-recreativo del sector para permitir el disfrute de los visitantes. En el primer capítulo, se estudian las características generales del atractivo turístico, considerando la descripción de la planta turística y de las actividades recreativas que se ofrecen en el club. En el segundo capítulo, por medio de la aplicación de encuestas se obtiene el perfil del visitante, y la determinación del nivel de satisfacción de los visitantes en relación a los servicios y actividades brindados por el club. En el último capítulo, en base a cálculos específicos se obtuvo la capacidad de carga Física, Real, Efectiva, y de Manejo del club Garza Roja. Además, se elaboró una matriz de evaluación de impactos para conocer la relación de las actividades recreativas entre los componentes sociales, y ambientales del lugar. Finalmente, con los resultados obtenidos se dan sugerencias para mejorar el funcionamiento del club

Microtoponyms in Late 17th Century Maps of Vidzeme: The Case of Sāviena (Sawensee) and Aburti (Lüggen) Estates

Vidzeme, one of the cultural and historical regions of Latvia, became a part of the kingdom of Sweden during the 17th century. Subsequently, Swedish cartographers mapped the estates of Vidzeme and then compiled maps of larger territories then called castle districts. The present research inspects 11 of these late 17th century maps and analyzes the microtoponyms found there. For an illustration, the maps of Sāviena (Sawensee) and Aburti (Lüggen) estates are chosen. This paper consider as microtoponyms not only the names of meadows, forests, swamps and hills, but also some hydronyms denoting smaller rivers and lakes that had not yet been academically analyzed, in total names of 26 objects. The majority of these microtoponyms denote what was then called streijgabali – remote parts of agricultural land that belonged to a central property but did not have a direct border with it. These maps depict some no longer existing lakes and swamps (e.g. Linn Siöö, Linne Purwe, Werdig purw), contain some almost unintelligible names of forests no longer known (e.g. Ewaete Sille), and testify about the ancient status of some place names (e.g. Pillis Kalln). The remote parts of land properties are often defined in map legends in Swedish as buskland i bruk, i.e. woodlands and scrublands. In most cases the names of these properties contain the generic element gabals ‘piece’. These placenames are often related to objects in the area. Four of them – Kanger, Pargull, Pasʃele and Saulleskaln – became farmhouse names during the following centuries. The number of these placenames is not sufficient to reconstruct the linguistic landscape of the 17th century estates. Nevertheless, they give an insight into the concepts of centre and periphery of that time and their denominations, into the perception of reference points of a landscape, and into the creative mind of the 17th century people

Silencing Motifs in the Clr2 Protein from Fission Yeast, Schizosaccharomyces pombe

The fission yeast, Schizosaccharomyces pombe, is a well-established model for heterochromatin formation, but the exact sequence of events for initiation remains to be elucidated. The essential factors involved include RNA transcribed from repeated sequences together with the methyltransferase Clr4. In addition, histone deacetylases, like Clr3, found in the SHREC complex are also necessary for transcriptional silencing. Clr2 is another crucial factor required for heterochromatin formation found in the SHREC complex. The function of Clr2 has been difficult to establish due to the lack of conserved domains or homology to proteins of known molecular function. Using a bioinformatics approach, three conserved motifs in Clr2 were identified, which contained amino acids important for transcriptional repression. Analysis of clr2 mutant strains revealed a major role for Clr2 in mating-type and rDNA silencing, and weaker effects on centromeric silencing. The effect on mating-type silencing showed variegation in several of the strains with mutated versions of Clr2 indicating an establishment or maintenance defect. Moreover, the critical amino acids in Clr2 were also necessary for transcriptional repression in a minimal system, by the tethering of Clr4 upstream of a reporter gene, inserted into the euchromatic part of the genome. Finally, in silico modeling suggested that the mutations in Clr2 cause disruption of secondary structures in the Clr2 protein. Identification of these critical amino acids in the protein provides a useful tool to explore the molecular mechanism behind the role of Clr2 in heterochromatin formation

<i>In silico</i> analysis of the conserved motifs C2SM1-3 and the consequences of introducing the mutations.

<p>3D molecular modeling study of the three conserved motifs in Clr2. C2SM1 (A), C2SM2 (B) and C2SM3 (C). Mutating the conserved amino acids in Clr2 was predicted to disrupt the secondary structure of the protein. Overlay between the predicted wild type structure in red and the simulated post-MD mutant protein conformation in green color.</p