Desenvolvimento de um jogo multiplataforma utilizando cross platform toolkit Haxe

Este trabalho apresenta o desenvolvimento de um jogo multiplataforma utilizando a biblioteca HaxeFlixel. O projeto foi especificado utilizando o Método Ágil Scrum. O jogo é constituído de duas modalidades com características diferentes de interação do jogador, onde a primeira modalidade possui características de RPG (Role Playing Game), onde tem-se a possibilidade de explorar cenários gerados aleatoriamente, já a segunda modalidade, consiste em enviar os heróis em uma série de missões de acordo com suas habilidades. Além disso, esse trabalho tem como objetivo compreender a complexidade do desenvolvimento de jogos, e as características e benefícios da linguagem Haxe para este ambiente.This work presents the development of a multiplataform game using the HaxeFlixel library. The game project was specified using the Agile Method Scrum. The game is going to feature two modalities with different forms of interaction by the player, where the first presents RPG (Role Playing Game) characteristics where is possible to explore random generated levels. The second modality consists in sending the heroes in a series of missions according to their abilities. Furthermore, this work aims to comprehend the complexity of game development and the characteristics and benefits of the Haxe language in this environment

The chaperone TRAP1 as a modulator of the mitochondrial adaptations in cancer cells

Mitochondria can receive, integrate, and transmit a variety of signals to shape many biochemical activities of the cell. In the process of tumor onset and growth, mitochondria contribute to the capability of cells of escaping death insults, handling changes in ROS levels, rewiring metabolism, and reprograming gene expression. Therefore, mitochondria can tune the bioenergetic and anabolic needs of neoplastic cells in a rapid and flexible way, and these adaptations are required for cell survival and proliferation in the fluctuating environment of a rapidly growing tumor mass. The molecular bases of pro-neoplastic mitochondrial adaptations are complex and only partially understood. Recently, the mitochondrial molecular chaperone TRAP1 (tumor necrosis factor receptor associated protein 1) was identified as a key regulator of mitochondrial bioenergetics in tumor cells, with a profound impact on neoplastic growth. In this review, we analyze these findings and discuss the possibility that targeting TRAP1 constitutes a new antitumor approach

Radio observations of peculiar galaxies

With the 1000-foot radiotelescope of the Arecibo Ionospheric Observatory we have observed the positions of 69 peculiar galaxies classified by Arp (1966). Several radio sources were detected, but they could be chance coincidences. Radio maps of the radio sources are given.Asociación Argentina de Astronomí

Radio observations of peculiar galaxies

With the 1000-foot radiotelescope of the Arecibo Ionospheric Observatory we have observed the positions of 69 peculiar galaxies classified by Arp (1966). Several radio sources were detected, but they could be chance coincidences. Radio maps of the radio sources are given.Asociación Argentina de Astronomí

Radio observations of peculiar galaxies

With the 1000-foot radiotelescope of the Arecibo Ionospheric Observatory we have observed the positions of 69 peculiar galaxies classified by Arp (1966). Several radio sources were detected, but they could be chance coincidences. Radio maps of the radio sources are given.Asociación Argentina de Astronomí

Perfiles verticales de metales en sedimentos del Río Uruguay, Argentina

Fil: Tatone, Leandro Martín. Laboratorio de Química Ambiental y Biogeoquímica. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Bilos, Claudio. Laboratorio de Química Ambiental y Biogeoquímica (LAQAB). Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Skorupka, Carlos Norberto. Laboratorio de Química Ambiental y Biogeoquímica (LAQAB). Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Colombo, Juan Carlos. Laboratorio de Química Ambiental y Biogeoquímica (LAQAB). Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; Argentin

Late Palaeozoic lithostratigraphy of the Andean Precordillera revisited (San Juan Province, Argentina)

New data on some neopalaeozoic lithostratigraphic units of the central sector of the Argentinean Precordillera are presented. The western Precordillera displays occasional outcrops of siliciclastic rocks of the Late Palaeozoic. These rocks are separated by two main unconformi­ties related to the development of the Chanic (Upper Devonian-early Carboniferous) and Gondwanan (late Carboniferous-early Permian) orogens. A major unconformity marks the beginning of the Andean cycle in middle Permian times. The Del Ratón Formation (800 m) in the studied area is made up of conglomerates and can be divided into two units. The basal part (Lower Member) was deposited in a fjord environment. The Upper Member was generated by an alluvial system that was mainly dominated by upper flow-regime plane beds, whereas its uppermost part accumulated in a fan delta setting. The clast provenance and the main palaeocurrents suggest the existence of a significant topographic high to the W and NW of the present outcrops. The El Planchón Formation (1400 m) is mainly composed of shales and sandstones as a result of sedimentation in a deep-sea fan environment. These lutite-dominated materials graded laterally north­wards into conglomerates in a glacial setting. The Del Ratón and El Planchón formations of the Lower Carboniferous correspond to the synorogenic Chanic sedimentation (Chanic foreland basin). Thereafter, the stratigraphic succession underwent deformation which led to its prolonged subaerial exposure and rubefaction. In Permian times, a marine transgression gave rise to the Del Salto Formation (600 m), which is constituted by beach deposits with some aeolian reworking and which lies unconformably over the Lower Carboniferous rocks. Conglomerates (Quebrada del Alumbre Formation, 100 m) produced by an alluvial system prograded episodically into coastal areas. The Escombrera Formation (350 m) is composed of beach deposits prograding seawards. This formation probably represents the end of the Palaeozoic sedimentary record. Thereafter, a playa-lake and an alluvial fan system were generated (Quebrada de la Arena Formation). This unit (750 m) was the last infill of the Gondwanan retroarc foreland, the total thickness of which was about 4000 m.Se presentan nuevos datos sobre las unidades neopaleozoicas aflorantes en la parte occidental de la Precordillera andina. Esas unidades litoestratigráficas estan separadas por dos discordancias angulares principales asociadas al desarrollo de las orogenias Chánica (Devónico tardío-Carbonífero temprano) y Gondwánica (Carbonífero tardío-Pérmico temprano) respectivamente. Otra nueva discordancia sugiere el inicio del ciclo andino durante el Pérmico medio. La Formación Del Ratón (800 m) está constituida principalmente por conglomerados y puede dividirse en dos unidades de rango menor: la parte basal y la parte superior respectivamente. La parte basal (Miembro inferior) fue acumulada en un fiordo. La parte superior (Miembro superior) fue generada en un sistema alluvial dominado predominantemente por la acumulación de capas planas de alto régimen de flujo, mientras que la parte más alta fue depositada en un entorno de abanico deltaico (fandelta). La procedencia de los clastos y las paleocorrientes principales indican la presencia de un relieve topográfico alto y significativo, situado al O y NO de los afloramientos actuales.La Formación El Planchón (1400 m) constituida principalmente por lutitas y areniscas fue acumulada en un contexto de abanico submarino (deep-sea fan). Estos materiales predominantemente lutíticos pasan lateralmente hacia el norte a conglomerados acumulados en un ambiente sedimentario glacial. Las Formaciones Del Ratón y El Planchón corresponden a la sedimentación sinorogénica Chánica (cuenca de antepaís chánica). Posteriormente, la sucesión estratigráfica sufrió una deformación que dió lugar a una prolongada exposición subaérea y su consiguiente rubefacción. Durante el Pérmico, una transgresión marina produjo la acumulación de la Formación Del Salto (600 m), que está constituída por depósitos de playa con algún retrabajamiento eólico y que recubre discordantemente los materiales del Carbonífero inferior. Un sistema aluvial conglomerático (Formación Quebrada del Alumbre 100 m) pogradó episódicamente sobre las zonas costeras. La Formación Escombrera (350 m), que está constituída por materiales arenosos coste-ros progradantes hacia el mar, representa, muy probablemente, el final del registro sedimentario paleozoico en esta zona. Posteriormente se generó otra discordancia sobre la que se acumularon materiales arenosos en un contexto de cursos fluviales trenzados sobre los que se disponen materiales lutíticos acumulados en una ciénaga (playa-lake) que gradan, primero a cursos trenzados, luego a cursos sinousos y finalmente a abanicos aluviales (Formación Quebrada de la Arena). Esta unidad (750 m) corresponde al último relleno sedimentario de la cuenca Gondwánica de retroarco, cuya potencia es del orden de unos 4000 m.Fil: Colombo, F.. Universidad de Barcelona; EspañaFil: Limarino, Carlos Oscar. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Geología; ArgentinaFil: Spalletti, Luis Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Geológicas. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Investigaciones Geológicas; ArgentinaFil: Busquets, P. Universidad de Barcelona; EspañaFil: Cardo, R.. Secretaría de Industria y Minería. Servicio Geológico Minero Argentino; ArgentinaFil: Mendez Bedia, I. Universidad de Oviedo; EspañaFil: Heredia, N.. Instituto Geológico y Minero de España; Españ

Dinámica de metales pesados en la laguna Inés (Bahía de Ñandubaysal, Río Uruguay)

Fil: Bilos, Claudio. Laboratorio de Química Ambiental y Biogeoquímica (LAQAB). Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Tatone, Leandro Martín. Laboratorio de Química Ambiental y Biogeoquímica (LAQAB). Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Skorupka, Carlos Norberto. Laboratorio de Química Ambiental y Biogeoquímica (LAQAB). Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Colombo, Juan Carlos. Laboratorio de Química Ambiental y Biogeoquímica (LAQAB). Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; Argentin

A novel source of cytoplasmic male sterility in Calibrachoa pubescens

Calibrachoa pubescens is a species native from the south of Brazil, Uruguay and northeast Argentina. An accession identified as 7.3.1.1 was collected at San Martín Department in Corrientes Province, Argentina and is included in the calibrachoa breeding program at the Institute of Floriculture, INTA. This accession is male sterile and produces male-sterile progeny, characterized by the lack of pollen production. Male sterility may be controlled by nuclear or cytoplasmic genes with each type presenting a different mode of inheritance. The objective of this research was to present a novel source of cytoplasmic male sterility in Calibrachoa pubescens. Crosses were made in the greenhouse between the male-sterile line 7.3.1.1 as the female parent and seven male-fertile lines of diverse origin. F1s were backcrossed and self-pollinated. Individual plants of the progenies were classified as male-fertile or male-sterile according to pollen viability. Analyses of observed segregations showed that male sterility observed in 7.3.1.1 results from the interaction of a male sterile cytoplasm and nuclear restorer genes. A novel male sterile cytoplasm is now available for breeders. Main advantages of cytoplasmic male sterility in ornamentals breeding are: efficient hybrid production, increased flower longevity, avoidance of pollen allergens and control of plant invasiveness.Inst. de Genética "Ewald A. Favret"- IGEAFFil: Colombo, Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Coviella, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Intistuto de Floricultura; ArgentinaFil: Hagiwara, Juan Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentin

New insights into enterocin CRL35: mechanism of action and immunity revealed by heterologous expression in Escherichia coli

The role of the class IIa bacteriocin membrane receptor protein remains unclear, and the following two different mechanisms have been proposed: the bacteriocin could interact with the receptor changing it to an open conformation or the receptor might act as an anchor allowing subsequent bacteriocin insertion and membrane disruption. Bacteriocin-producing cells synthesize an immunity protein that forms an inactive bacteriocin–receptor–immunity complex. To better understand the molecular mechanism of enterocin CRL35, the peptide was expressed as the suicidal probe EtpM-enterocin CRL35 in Escherichia coli, a naturally insensitive microorganism since it does not express the receptor. When the bacteriocin is anchored to the periplasmic face of the plasma membrane through the bitopic membrane protein, EtpM, E. coli cells depolarize and die. Moreover, co-expression of the immunity protein prevents the deleterious effect of EtpM-enterocin CRL35. The binding and anchoring of the bacteriocin to the membrane has demonstrated to be a sufficient condition for its membrane insertion. The final step of membrane disruption by EtpM-enterocin CRL35 is independent from the receptor, which means that the mannose PTS might not be involved in the pore structure. In addition, the immunity protein can protect even in the absence of the receptor.Fil: Barraza, Daniela Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Ríos Colombo, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Galván, Adriana Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Acuña, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Minahk, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Chalon, Miriam Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentin