Analytical chemistry and metrological issues related to nonylphenols in environmental health

International audienceIn recent decades, the development of environmental risks and their repercussions on health has led to environmental health being a field of scientific research in which interdisciplinarity is intrinsic. This article on nonylphenols (NP) shows how exchanges and knowledge transfer involving chemists, biologists, pharmacists and physicians underscore the need to further the development of analytical methodologies. We spell out the difficulties encountered when selecting a reference material for the analysis of NP (i.e. multiplicity of isomers, and variability in the composition of batches for the same CAS Registry Number). As a result, we propose 353NP (CAS 186825-36-5) as the reference material (because of its high proportion in the industrial mixture and its pronounced estrogenic power)

Reliable quantification of bisphenol A and its chlorinated derivatives in human breast milk using UPLC-MS/MS method

International audienceBisphenol A is a widespread industrial chemical which over the past decade has demonstrated its toxicity as an endocrine disruptor. Chlorine present in drinking water may react with bisphenol A to form chlorinated derivatives, which have demonstrated a heightened level of estrogenic activity. In this work, we have comprehensively validated a method using on-line SPE-UPLC-MS/MS and isotope dilution quantification to measure bisphenol A and its chlorinated derivatives in human breast milk according to accepted guidelines. Deutered bisphenol A was used as internal standard. The matrix calibration curve ranged from 0.40 to 6.40 ng/mL for each of the target compounds and provided good linearity (r(2) > 0.99). This method was precise (the intra and inter-day coefficient of variation was <20% at two different concentrations (0.40 and 3.20 ng/mL) and accurate (recovery ranged from 81% to 119%). The limits of detection obtained for BPA and its chlorinated derivatives ranged from 0.01 to 0.09 ng/ml.. The limit of quantification for all the compounds validated at 0.40 ng/mL when using 500 mu L of milk was found to be suitable for the concentration existing in real samples. The analytical method developed in this study is in accordance with the requirements applicable to biomonitoring of BPA and its chlorinated derivatives in human breast milk

Analytical chemistry and metrological issues related to nonylphenols in environmental health

In recent decades, the development of environmental risks and their repercussions on health has led to environmental health being a field of scientific research in which interdisciplinarity is intrinsic. This article on nonylphenols (NP) shows how exchanges and knowledge transfer involving chemists, biologists, pharmacists and physicians underscore the need to further the development of analytical methodologies. We spell out the difficulties encountered when selecting a reference material for the analysis of NP (i.e. multiplicity of isomers, and variability in the composition of batches for the same CAS Registry Number). As a result, we propose 353NP (CAS 186825-36-5) as the reference material (because of its high proportion in the industrial mixture and its pronounced estrogenic power)

Amélioration de la couverture vaccinale anti-pneumococcique chez le sujet âgé

To study pneumococcal immunization coverage in older patients in hospital, and the impact of two actions aiming at improving this coverage