Effect of vacuum level on milk flow traits in Mediterranean Italian buffalo cow

The aim of this study was to determine the effects of six different working vacuum levels (range from 37 to 52 kPa) on the milk production, milk flow rate and milking times in Mediterranean Italian buffalo. A total of eight hundred and one milk flow curves were recorded at random from all of the four hundred and fifty animals in different parity and stage of lactation over a period of 12 weeks, with electronic milk flow meters (Lactocorder®). The different vacuum levels tested did not affect significantly the individual milk production per milking (on average 4.02±0.06 kg). When diminishing vacuum level, a decrease in average and peak flow rate occurred (P<0.001), as well as an increase in effective milking time between attaching the teat cup and reaching the value of 0.20 kg/min at the end of milking (P<0.001). The vacuum levels of 37 and 40 kPa showed good milkability conditions, at which plateau phase was longer than decline phase and lag time was not affected by vacuum level

Optimizing detection of RET and PPARg rearrangements in thyroid neoplastic cells using a home-brew tetracolor probe

BACKGROUND Fluorescence in situ hybridization (FISH) to identify specific DNA target sequences in the nuclei of nondividing cells of numerous solid neoplasms has contributed to the introduction of molecular cytogenetics as a useful adjunct to cytology, leading recently to the "marriage" of the 2 disciplines. Numerous cancer molecular markers can now be investigated using different technical approaches, at both the gene and expression levels, in biopsies of various suspected cancers, including differentiated thyroid carcinoma. The limited amount of bioptic material is often insufficient to carry out multiple tests, and optimizing handling of the biopsy is desirable. METHODS We have developed a home-brew tetracolor break-apart probe able to simultaneously identify the 2 most common genetic alterations in differentiated thyroid carcinoma: RET/PTC variants in papillary thyroid carcinoma and PAX8/PPARg fusion and variants in follicular thyroid carcinoma. RESULTS The probe had 100% specificity, 99.5% sensitivity, and >= 3% cutoff. The probe was tested on RET/PTC and PAX8/PPARg RT-PCR positive controls, and feasibility was assessed in 368 thyroid nodule fine-needle aspirations (FNA). In the latter analysis, 24 FNAs had split RET signal, and 9 had split PPARg signal. FISH analysis of available surgically removed nodules confirmed the sensitivity of FISH in detecting abnormal clones and oligoclones. CONCLUSIONS The home-brew tetracolor probe showed high feasibility, optimizing the use of the biological material in relation to the available molecular tests and maximizing the FISH experimental and slide-scoring times. This probe may be considered an alternative to RT-PCR when recovery and quality of RNA amplification from FNA are insufficient

Efficient second harmonic generation from thin films of V-shaped benzo[b]thiophene based molecules

We have designed an original approach for efficient Second Harmonic Generation of tailored V-shape benzo[b]thiophene molecular systems enabling versatile and flexible one-step, dry and technologically friendly thin film processing. The designed moieties show χ(2) values at least as high as the reference LiNbO3 single crystal, without poling processing and matching the constrains of integrated optical configuration for nonlinear optical devices. This may open the way to a new class of organic materials exploitable for photonic applications

Neurofeedback: principles, appraisal and outstanding issues

Neurofeedback is a form of brain training in which subjects are fed back information about some measure of their brain activity which they are instructed to modify in a way thought to be functionally advantageous. Over the last twenty years, NF has been used to treat various neurological and psychiatric conditions, and to improve cognitive function in various contexts. However, despite its growing popularity, each of the main steps in NF comes with its own set of often covert assumptions. Here we critically examine some conceptual and methodological issues associated with the way general objectives and neural targets of NF are defined, and review the neural mechanisms through which NF may act, and the way its efficacy is gauged. The NF process is characterised in terms of functional dynamics, and possible ways in which it may be controlled are discussed. Finally, it is proposed that improving NF will require better understanding of various fundamental aspects of brain dynamics and a more precise definition of functional brain activity and brain-behaviour relationships.Comment: 12 page

Inhibition of enterovirus a71 by a novel 2-phenyl-benzimidazole derivative

Enterovirus A71 (EV-A71) infection has emerged as a significant public health concern at the global level. Epidemic events of EV-A71 have been reported worldwide, and this succession of outbreaks has heightened concern that EV-A71 may become a public health threat. In recent years, widespread A71 enterovirus also occurred in European countries. EV-A71 infection causes hand-foot-mouth disease (HFMD), herpangina, and fever. However, it can sometimes induce a variety of neurological complications, including encephalitis, aseptic meningitis, pulmonary edema, and acute flaccid paralysis. We identified new benzimidazole derivatives and described theirin vitrocytotoxicity and broad-spectrum anti-enterovirus activity. Among them, derivative 2b resulted in interesting activity against EV-A71, and therefore it was selected for further investigations. Compound 2b proved to be able to protect cell monolayers from EV-A71-induced cytopathogenicity, with an EC50 of 3 µM. Moreover, Vero-76 cells resulted in being significantly protected from necrosis and apoptosis when treated with 2b at 20 and 80 µM. Compound 2b reduced viral adsorption to Vero-76 cells, and when evaluated in a time-of-addition assay, the derivative had the highest effect when added during the infection period. Moreover, derivative 2b reduced viral penetration into host cells. Besides, 2b did not affect intestinal monolayers permeability, showing no toxic effects. A detailed insight into the efficacy of compound 2b against EV-A71 showed a dose-dependent reduction in the viral titer, also at low concentrations. Mechanism of action investigations suggested that our derivative can inhibit viral endocytosis by reducing viral attachment to and penetration into host cells. Pharmacokinetic and toxicity predictions validated compound 2b as a good candidate for furtherin vivoassays

Foramen caróticoclinoideo en cráneos humanos: incidencia, morfometría y sus implicaciones clínicas

The caroticoclinoid foramen is an inconstant structure, formed by the union of the anterior and middle clinoid processes. The aim of this study was to perform an incidence and morphometry of the caroticoclinoid foramen in Brazilian human skulls and discuss its clinical implications. Eighty dry human skulls with sex distinction were used, and 3 groups of incidence were determined: General, sex, and sides. The morphometry was performed using a manual caliper and the major diameter of the foramina was measured; the values were also divided in general, according to sex and sides. The incidence of skulls with at least one foramen was 8.5%. According to the sides, 8.5% of the skulls showed foramen on the right side and 2.5% on the left. We found 2.5% of the skulls with bilateral foramen and 6.25% with unilateral foramen. In relation to sex, the foramens were found in 5% of male skulls and 12.5% of female skulls. The major diameter of this structure presented on mean, values of 5.23 mm on general, 5.18 mm on the right side and 5.35 mm on the left, 5.30 mm in male skulls and 5.18 mm in female skulls. The anatomical characteristics of this foramen should be considered in view of its clinical implications associated with neurosurgery as clinoid process removal, and symptoms as headache due to internal carotid artery alterations in this region. In conclusion knowledge of this structure supports the diagnosis and treatment of clinical complications related to this variation.The caroticoclinoid foramen is an inconstant structure, formed by the union of the anterior and middle clinoid processes. The aim of this study was to perform an incidence and morphometry of the caroticoclinoid foramen in Brazilian human skulls and discuss its clinical implications. Eighty dry human skulls with sex distinction were used, and 3 groups of incidence were determined: General, sex, and sides. The morphometry was performed using a manual caliper and the major diameter of the foramina was measured; the values were also divided in general, according to sex and sides. The incidence of skulls with at least one foramen was 8.5%. According to the sides, 8.5% of the skulls showed foramen on the right side and 2.5% on the left. We found 2.5% of the skulls with bilateral foramen and 6.25% with unilateral foramen. In relation to sex, the foramens were found in 5% of male skulls and 12.5% of female skulls. The major diameter of this structure presented on mean, values of 5.23 mm on general, 5.18 mm on the right side and 5.35 mm on the left, 5.30 mm in male skulls and 5.18 mm in female skulls. The anatomical characteristics of this foramen should be considered in view of its clinical implications associated with neurosurgery as clinoid process removal, and symptoms as headache due to internal carotid artery alterations in this region. In conclusion knowledge of this structure supports the diagnosis and treatment of clinical complications related to this variation292427431Das, S., Suri, R., Kapur, V., Ossification of caroticoclinoid ligament and its clinical importance in skull-based surgery (2007) São Paulo Med. J, 125, pp. 351-353Dodo, Y., Ishida, H., Incidence of nonmetric cranial variant in several population samples from East Asia and North America (1987) J. Anthrop. Soc. Nippon, 95, pp. 161-167Dolenc, V.V., A combined epi and subdural direct approach to carotidophthalmic artery aneurysms (1985) J. Neurosurg, 5, pp. 667-672Donald, P.J., (1998) Surgery of the Skull Base, , Philadelphia, Lippincott- RavenErturk, M., Kayalioglu, G., Govsa, F., Anatomy of the clinoidal region with special emphasis on the caroticoclinoid foramen and interclinoid osseous brid- ge in a recent Turkish population (2004) Neurosurg. Rev, pp. 22-26Gupta, N., Ray, B., Ghosh, S., A study on anterior clinoid process and optic strut with emphasis on variations of caroticoclinoid foramen (2005) Nep. Med. Coll. J, 7, pp. 140-144Hochstetter, F., Über die Taenia interclinoidea,die Commissura alicochlearis und die Cartilago supracochlearis des menschlichen Primordialkraniums.Gegenbaurs Morph (1940) Jahrb, 84, pp. 220-243Inoue, T., Rhoton Jr., A.L., Theele, D., Barry, M.E., Surgical approaches to the cavernous sinus:A microsurgical study (1990) Neurosurg, 6, pp. 903-932Kier, E.L., Embryology of the normal optic canal and its anomalies.An anatomic and roentgenographic stud (1966) Invest. Radiol, 1, pp. 346-362Kobayashi, S., Kyoshima, K., Gibo, H., Hedge, S.A., Takemae, T., Sugita, K., Carotid cave aneurysms of the internal carotid artery (1989) J. Neurosurg, 70, p. 216Lee, H.Y., Chung, I.H., Choi B., Y., Anterior, C., (1997) Yonsei Med. J, 38, pp. 151-154Narolewski, R., Significance of anatomic variants of bony surroundings of the internal carotid artery and their significance for lateral surgical approaches to the cavernous sinus (2003) Ann. Acad. Med. Stetin, 49, pp. 205-229Saka, E., Ozdoǧmus, O., Tulay, C., Gürdal, E., Uzün, I., The anatomy of the carotico-clinoid foramen and its relation with the internal carotid artery (2003) Surg. Radiol. Anat, 25, pp. 241-246Reisch, R., Vutskits, L., Filippi, R., Patonay, L., Fries, G., Perneczky, A., Topographic microsurgical anatomy of the paraclinoid carotid artery (2002) Neurosurg Rev, 25, pp. 177-183Sekhar, L.N., Akin, O., Anatomical study of the cavernous sinus emphasizing operative approaches and related vascular and neural reconstruction (1987) Neurosurg, 21, pp. 806-816Seoane, E., Rhoton, A.L., Oliveira, E., Microsurgical anatomy of the dural collar (carotid collar) and rings around the clinoid segment of the internal carotid artery (1998) Neurosurg, 42, pp. 869-886Sicher, H., du Brul, E.L., (1977) Oral Anatomy, , 6ed. Rio de Janeiro, Guanabara KooganStandring, S., Overview of the Development of the Head and Neck Head: Skull and Mandible Gray's anatomy: The anatomical basis of clinical practice, p. 2005. , New York, ElsevierEl foramen caróticoclinoideo es una estructura inconstante formada por la unión de la procesos clinoides anterior y medio. El objetivo de este estudio fue revisar la incidencia y la morfometría del foramen caróticoclinoideo en cráneos humanos brasileños y discutir sus implicaciones clínicas. Se utilizaron 80 cráneos humanos secos, de ambos sexos, determinando tres variables: sexo y lados derecho e izquierdo. La morfometría se realizó con un cáliper manual midiéndose el diámetro máximo del foramen. La incidencia de cráneos con al menos un foramen fue de 8,5%. El 8,5% de los cráneos presentó un foramen en el lado derecho y el 2,5% en el izquierdo. El 2,5% de los cráneos presentó un foramen bilateral y el 6,25% un foramen unilateral. En relación al sexo, los forámenes se encontraron en el 5% de los cráneos de hombres y el 12,5% de los cráneos de mujeres. El diámetro máximo de esta estructura presentó en promedio, valores de 5,23 mm; 5,18 mm en el lado derecho y 5,35 mm en el izquierdo; 5,30 mm en cráneos de hombres y 5.18 mm en cráneos de mujeres. Las características anatómicas de este foramen deben ser consideradas debido a las implicaciones clínicas asociadas a la neurocirugía, como la eliminación del proceso clinoides y a síntomas como cefaleas producto de alteraciones en la arteria carótida interna en esta región. En conclusión, el conocimiento de esta estructura mejora el diagnóstico y tratamiento de las complicaciones clínicas relacionadas con esta variació

Relaciones entre el canal mandibular y clases I, II y III de Angle en radiografías panorámicas

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOTo prevent injury to the inferior alveolar nerve during dental procedures, knowledge of its anatomical location and course of the mandibular canal is imperative. The aim of this study was evaluate the location on panoramic radiographs of the mandibular canal in relation to the apices of the permanent mandibular molars and base mandible, and relate the type of mandibular canal with Angle classes I, II and III. We evaluated 748 panoramic radiographs distributed according to sex and occlusal class (Angle I, II and III) of fully dentate individuals, 18-51 age group. The radiographs were divided according to Angle classes based on cephalometric tracing, clinical data from the medical records of each individual and the analysis of of the maxillar and mandibular dental arches models. The same individuals had the mandibular canal bilaterally assessed, and classified according to their location relative to the root apices of the mandibular molars and mandible base. Measurements of the distance from the mandibular canal to the apices of the teeth and mandible base were made on the Software ImageLab2000®. The intra-observer reproducibility of measurements on radiographs was assessed using the coefficient of variation (p<0.0001). Data were submitted to Kruskal-Wallis test, on software BioEstat 5.0. There were statistically significant differences (Kruskal-Wallis test, p <0.0001) between the occlusal classes, with the type of mandibular canal. A larger number of canals types 2 and 3 in class III individuals than in others. In conclusion, the location of the mandibular canal presents morphological changes in relation to the apices of the permanent molars, and the mandibular base according to the Angle classes I, II and III.To prevent injury to the inferior alveolar nerve during dental procedures, knowledge of its anatomical location and course of the mandibular canal is imperative. The aim of this study was evaluate the location on panoramic radiographs of the mandibular canal in relation to the apices of the permanent mandibular molars and base mandible, and relate the type of mandibular canal with Angle classes I, II and III. We evaluated 748 panoramic radiographs distributed according to sex and occlusal class (Angle I, II and III) of fully dentate individuals, 18-51 age group. The radiographs were divided according to Angle classes based on cephalometric tracing, clinical data from the medical records of each individual and the analysis of of the maxillar and mandibular dental arches models. The same individuals had the mandibular canal bilaterally assessed, and classified according to their location relative to the root apices of the mandibular molars and mandible base. Measurements of the distance from the mandibular canal to the apices of the teeth and mandible base were made on the Software ImageLab2000®. The intra-observer reproducibility of measurements on radiographs was assessed using the coefficient of variation (p<0.0001). Data were submitted to Kruskal-Wallis test, on software BioEstat 5.0. There were statistically significant differences (Kruskal-Wallis test, p <0.0001) between the occlusal classes, with the type of mandibular canal. A larger number of canals types 2 and 3 in class III individuals than in others. In conclusion, the location of the mandibular canal presents morphological changes in relation to the apices of the permanent molars, and the mandibular base according to the Angle classes I, II and III322449454FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOsem informaçãoPara evitar lesiones del nervio alveolar inferior durante los procedimientos odontológicos, el conocimiento de la localización anatómica y curso del canal mandibular (CM) es imprescindible. El objetivo fue evaluar la localización del CM sobre radiografías panorámicas en relación con los ápices de los molares mandibulares permanentes y la base mandibular, relacionando el tipo de CM con las clases I, II y III de Angle. Se evaluaron 748 radiografías panorámicas distribuidas según sexo y clase oclusal (I, II y III de Angle) en individuos totalmente dentados (edad entre 18-51 años). Las radiografías fueron divididas de acuerdo a las clases de Angle según su trazado cefalométrico, datos clínicos de los registros médicos de cada individuo y el análisis de modelos de sus arcos dentarios mandibulares y maxilares. En los individuos se evaluó el CM bilateralmente y se clasificó de acuerdo a su ubicación en relación con los ápices radiculares de los molares mandibulares y base mandibular. Las mediciones de distancia desde el CM a los ápices radiculares y base mandibular se hicieron con el programa ImageLab2000®. Se evaluó la reproducibilidad intra-observador de las mediciones en las radiografías usando el coeficiente de variación (p<0,0001). Los datos fueron sometidos a la prueba de Kruskal ­Wallis con el programa BioEstat 5.0. Se observaron diferencias estadísticamente significativas (prueba de Kruskal-Wallis, p<0,0001) entre las clases oclusales y el tipo de CM. Hubo mayor número de canales tipos 2 y 3 en los individuos clase III. La ubicación del canal mandibular presenta cambios morfológicos en relación con los ápices de molares permanentes y la base mandibular de acuerdo a las clases I, II y III de Angl

Morfometría de la apertura piriforme y morfología de los huesos nasales en la población brasileña por la radiografía postero-anterior de Caldwell

CAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL E NÍVEL SUPERIORCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOPiriform aperture and nasal bone contribute to the formation of the nose. Its morphology varies according to ethnic group and gender. The piriform apertures and nasal bones of 97 individuals were examined radiographically through Caldwell radiographic technique and the types of nasal bones were classified according to Hwang et al. (2005). The width, height and area of piriform aperture were measured to demonstrate the difference according to gender. Statistical analysis was performed from the Student t test and analysis of variance (ANOVA). The most frequent type of nasal bone was A, and D was the most uncommon in both genders. In the Student t test significant differences between genders, height, width and area of the piriform aperture were obtained and were larger in males when compared to females. The dimensions of the piriform aperture were higher in males when compared to females, and therefore may be used as parameter for the distinction of gender in Brazilian population. The type A of nasal bone was the most frequent in the Brazilian population, contributing to ethnic differentiation in human identification292393398CAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL E NÍVEL SUPERIORCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL E NÍVEL SUPERIORCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOsem informaçãosem informaçãoLa apertura piriforme y el hueso nasal contribuyen a la formación de la nariz. Su morfología varía de acuerdo a los grupos étnicos y al sexo. Este estudio evaluó la morfología de la apertura piriforme y el hueso nasal y su relación con el sexo. Las aperturas piriformes y los huesos nasales de 97 personas fueron examinados radiográficamente mediante la técnica radiográfica de Caldwell, y los tipos de huesos nasales se clasificaron de acuerdo con Hwang et al. (2005). Se midió el ancho, la altura y el área de la apertura piriforme para demostrar las diferencias por sexo. El análisis estadístico se realizó a partir de la prueba t de Student y análisis de varianza (ANOVA). El tipo más frecuente de hueso nasal fue la A, y la D fue la menos común, en ambos sexos. En la prueba t de Student se obtuvo diferencias significativas entre los sexos, altura, ancho y área de la apertura piriforme, las que fueron mayores en hombres en comparación a las mujeres. Las dimensiones de la apertura piriforme fueron mayores en los hombres que en las mujeres, por lo que se puede utilizar como parámetro para la distinción de sexo en la población brasileña. En la población brasileña el tipo A de hueso nasal fue el más frecuente, lo que contribuye para la diferenciación étnica en la identificación human