Isolation, Characterization and Lipid-Binding Properties of the Recalcitrant FtsA Division Protein from Escherichia coli

We have obtained milligram amounts of highly pure Escherichia coli division protein FtsA from inclusion bodies with an optimized purification method that, by overcoming the reluctance of FtsA to be purified, surmounts a bottleneck for the analysis of the molecular basis of FtsA function. Purified FtsA is folded, mostly monomeric and interacts with lipids. The apparent affinity of FtsA binding to the inner membrane is ten-fold higher than to phospholipids, suggesting that inner membrane proteins could modulate FtsA-membrane interactions. Binding of FtsA to lipids and membranes is insensitive to ionic strength, indicating that a net contribution of hydrophobic interactions is involved in the association of FtsA to lipid/membrane structures

A first AFLP-based genetic linkage map for brine shrimp Artemia franciscana and its application in mapping the sex locus

We report on the construction of sex-specific linkage maps, the identification of sex-linked markers and the genome size estimation for the brine shrimp Artemia franciscana. Overall, from the analysis of 433 AFLP markers segregating in a 112 full-sib family we identified 21 male and 22 female linkage groups (2n = 42), covering 1,041 and 1,313 cM respectively. Fifteen putatively homologous linkage groups, including the sex linkage groups, were identified between the female and male linkage map. Eight sex-linked AFLP marker alleles were inherited from the female parent, supporting the hypothesis of a WZ-ZZ sex-determining system. The haploid Artemia genome size was estimated to 0.93 Gb by flow cytometry. The produced Artemia linkage maps provide the basis for further fine mapping and exploring of the sex-determining region and are a possible marker resource for mapping genomic loci underlying phenotypic differences among Artemia species

Ground penetrating radar and electrical resistivity tomography investigations in the southern sector of the Roman Forum: First results on the pre‐Augustan phases of the Basilica Julia

A research project aimed at the study of the archaeological context of the Basilica Julia in the Roman Forum (Rome, Italy) and the transformation phases of the building involved the use of different techniques of geophysical prospecting. In particular, Ground-penetrating Radar (GPR) and Electrical Resistivity Tomography (ERT) surveys were carried out in order to record the buried structures of the Basilica and the previous buildings laying in the southern part of the Forum. These geophysical methods have been chosen in order to guarantee a good compromise between resolution (GPR in the shallow subsurface) and depth of investigation (ERT in the deeper subsurface), with the aim of investigating up to a depth of about 10 m in a very conductive subsoil. In particular, GPR surveys were conducted in the five aisles of the monument, while ERT measurements were performed with nonstandard acquisition mode in order to include the entire perimeter of the building. These techniques allowed us to acquire new data on the structures buried in the sectors of the monumental complex that have never been excavated and on the paleosoil till a depth of about 10 m from the surface. Interesting data were acquired on the Basilica Julia itself, built during the late Republican period (54–46 BC) and rebuilt at the beginning of the first century AD, such as the technical features of the caementicium and travertine foundations, their relationship with the Cloaca Maxima and the traces of a design change occurred during the construction in the central aisle of the building; other buried remains of the previous Basilica Sempronia, constructed in 169 BC in the same area, were identified. Moreover, the investigations documented the paleosoil in the area between the Forum and the Velabrum to the south, between 7.5–8.5 and 5.5 m a.s.l. These data confirmed the general geological setting of the area, obtained from previous coring, but significantly specified the geomorphology of this border sector of the Roman Forum, which slopes down towards the south and the wes