198 research outputs found
Equilibrium and dynamical properties of two dimensional self-gravitating systems
A system of N classical particles in a 2D periodic cell interacting via
long-range attractive potential is studied. For low energy density a
collapsed phase is identified, while in the high energy limit the particles are
homogeneously distributed. A phase transition from the collapsed to the
homogeneous state occurs at critical energy U_c. A theoretical analysis within
the canonical ensemble identifies such a transition as first order. But
microcanonical simulations reveal a negative specific heat regime near .
The dynamical behaviour of the system is affected by this transition : below
U_c anomalous diffusion is observed, while for U > U_c the motion of the
particles is almost ballistic. In the collapsed phase, finite -effects act
like a noise source of variance O(1/N), that restores normal diffusion on a
time scale diverging with N. As a consequence, the asymptotic diffusion
coefficient will also diverge algebraically with N and superdiffusion will be
observable at any time in the limit N \to \infty. A Lyapunov analysis reveals
that for U > U_c the maximal exponent \lambda decreases proportionally to
N^{-1/3} and vanishes in the mean-field limit. For sufficiently small energy,
in spite of a clear non ergodicity of the system, a common scaling law \lambda
\propto U^{1/2} is observed for any initial conditions.Comment: 17 pages, Revtex - 15 PS Figs - Subimitted to Physical Review E - Two
column version with included figures : less paper waste
Discovery of amivantamab (JNJ-61186372), a bispecific antibody targeting EGFR and MET
A bispecific antibody (BsAb) targeting the epidermal growth factor receptor (EGFR) and mesenchymal-epithelial transition factor (MET) pathways represents a novel approach to overcome resistance to targeted therapies in patients with nonsmall cell lung cancer. In this study, we sequentially screened a panel of BsAbs in a combinatorial approach to select the optimal bispecific molecule. The BsAbs were derived from different EGFR and MET parental monoclonal antibodies. Initially, molecules were screened for EGFR and MET binding on tumor cell lines and lack of agonistic activity toward MET. Hits were identified and further screened based on their potential to induce untoward cell proliferation and crossphosphorylation of EGFR by MET via receptor colocalization in the absence of ligand. After the final step, we selected the EGFR and MET arms for the lead BsAb and added low fucose Fc engineering to generate amivantamab (JNJ-61186372). The crystal structure of the anti-MET Fab of amivantamab bound to MET was solved, and the interaction between the two molecules in atomic details was elucidated. Amivantamab antagonized the hepatocyte growth factor (HGF)-induced signaling by binding to MET Sema domain and thereby blocking HGF beta-chain-Sema engagement. The amivantamab EGFR epitope was mapped to EGFR domain III and residues K443, K465, I467, and S468. Furthermore, amivantamab showed superior antitumor activity over small molecule EGFR and MET inhibitors in the HCC827-HGF in vivo model. Based on its unique mode of action, amivantamab may provide benefit to patients with malignancies associated with aberrant EGFR and MET signaling.Transplantation and autoimmunit
Efficacy of piperine in reducing the effects of aflatoxin intoxication in broiler chickens: a preliminary report
Dependência térmica da germinação de sementes de Drymaria cordata (L.) Willd. ex Roem. & Schult. (Cariophyllaceae)
Building The Sugarcane Genome For Biotechnology And Identifying Evolutionary Trends
Background: Sugarcane is the source of sugar in all tropical and subtropical countries and is becoming increasingly important for bio-based fuels. However, its large (10 Gb), polyploid, complex genome has hindered genome based breeding efforts. Here we release the largest and most diverse set of sugarcane genome sequences to date, as part of an on-going initiative to provide a sugarcane genomic information resource, with the ultimate goal of producing a gold standard genome.Results: Three hundred and seventeen chiefly euchromatic BACs were sequenced. A reference set of one thousand four hundred manually-annotated protein-coding genes was generated. A small RNA collection and a RNA-seq library were used to explore expression patterns and the sRNA landscape. In the sucrose and starch metabolism pathway, 16 non-redundant enzyme-encoding genes were identified. One of the sucrose pathway genes, sucrose-6-phosphate phosphohydrolase, is duplicated in sugarcane and sorghum, but not in rice and maize. A diversity analysis of the s6pp duplication region revealed haplotype-structured sequence composition. Examination of hom(e)ologous loci indicate both sequence structural and sRNA landscape variation. A synteny analysis shows that the sugarcane genome has expanded relative to the sorghum genome, largely due to the presence of transposable elements and uncharacterized intergenic and intronic sequences.Conclusion: This release of sugarcane genomic sequences will advance our understanding of sugarcane genetics and contribute to the development of molecular tools for breeding purposes and gene discovery. © 2014 de Setta et al.; licensee BioMed Central Ltd.151European Commission: Agriculture and Rural Development: Sugar http://ec.europa.eu/agriculture/sugar/index_en.htmKellogg, E.A., Evolutionary history of the grasses (2001) Plant Physiol, 125, pp. 1198-1205Grivet, L., Arruda, P., Sugarcane genomics: depicting the complex genome of an important tropical crop (2001) Curr Opin Plant Biol, 5, pp. 122-127Piperidis, G., Piperidis, N., D'Hont, A., Molecular cytogenetic investigation of chromosome composition and transmission in sugarcane (2010) Mol Genet Genomics, 284, pp. 65-73D'Hont, A., 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Relatos de músicos cegos: subsídios para o ensino de música para alunos com deficiência visual
Crescimento de milheto e guandu, desempenho de plantas cítricas e propriedades físicas do solo escarificado em um pomar
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