RELAÇÃO ESCOLA E UNIVERSIDADE: REFLEXÕES A PARTIR DO PROJETO “LER & EDUCAR”

Fazemos parte do projeto “Ler & Educar: formação continuada de professores da rede pública de SC” (OBEDUC/CAPES), com sede na Universidade do Extremo Sul Catarinense (UNESC)

Genetic characterization of native and introduced populations of the neotropical cichlid genus Cichla in Brazil

A molecular phylogenetic analysis based on mitochondrial 16S ribosomal DNA and Control Region sequences from native and introduced populations was undertaken, in order to characterize the introduction of Cichla (peacock bass or tucunaré) species in Brazil. Mitochondrial DNA haplotypes found in introduced fish from Minas Gerais state (southeastern Brazil) clustered only with those from native species of the Tocantins River (Cichla piquiti and C. kelberi), thereby suggesting a single or, at most, few translocation acts in this area, even though with fish from the same source-population. Our study contributes to an understanding of the introduction of Cichla in regions of Brazil outside the Amazon basin, and adds phylogenetic data to the recently describe Cichla species, endemic from the Tocantins-Araguaia basin

Sunflower meal and supplementation of enzyme complex in diets for growing and finishing pigs

Este estudo teve como objetivo avaliar a inclusão de farelo de girassol e a suplementação de complexo enzimático sobre os parâmetros de desempenho e características de carcaça de suínos, dos 30 aos 100 kg de peso vivo. Foram utilizados 96 suínos com peso vivo médio de 32,19±3,27 kg, distribuídos em um delineamento experimental de blocos casualizados, em esquema fatorial 4 x 2 (quatro níveis de farelo de girassol-FG: 0, 8, 16 e 24% com ou sem inclusão do complexo enzimático-CE), com seis repetições e dois animais por unidade experimental. As variáveis analisadas foram o consumo de ração (kg), o ganho de peso (kg), a conversão alimentar (kg/ kg), a espessura de toucinho (mm), a musculosidade (kg), o peso da carcaça quente (%), a porcentagem de carne magra na carcaça (%), a quantidade de carne magra na carcaça (kg). Não houve nenhuma interação entre os fatores para nenhuma das variáveis estudadas. A conversão alimentar dos animais dos 30 aos 70 kg de peso foi diminuída pela inclusão do CE, porém não afetou os parâmetros de carcaça. Níveis crescentes de FG na ração apresentaram efeito quadrático sobre o ganho de peso dos animais e sobre a espessura de toucinho, com valores máximos dessas variáveis em 7,26% e 8,16% de inclusão do FG, respectivamente. Aiming to evaluate the performance of pigs from 30 to 100 kg of live a total of 96 pigs with average live weight of 32.19±3.27 kg were distributed in randomized blocks design in a 4 × 2 (four levels of SM: 0, 8, 16 and 24%; with or without inclusion of EC)  factorial arrangement with six replicates and two animals per experimental unit. The parameters analyzed were feed intake (kg), weight gain (kg), feed conversion (kg/kg), backfat thickness (mm), carcass muscularity (kg), hot carcass weight (%), percentage of lean meat in the carcass (%), and quantity of lean meat in the carcass (kg). There was no interaction between EC and SM levels in the diet. The addition of EC in the diet affected the performance of the animals, but not carcass characteristics. Feed conversion of animals from 30 to 70 kg weight was improved by the inclusion of EC. The increasing levels of SM in the diet presented quadratic effect on weight gain and on backfat thickness, with inclusion points of SM that promoted maximum values of these parameters of 7.26% and 8.16%, respectively.

Serum IGF-1 is insufficient to restore skeletal size in the total absence of the growth hormone receptor

States of growth hormone (GH) resistance, such those observed in Laron dwarf patients, are characterized by mutations in the GH receptor (GHR), decreased serum and tissue IGF-1 levels, impaired glucose tolerance, and impaired skeletal acquisition. IGF-1 replacement therapy in such patients increases growth velocity but does not normalize growth. Herein we combined the GH-resistant (GHR knockout [GHRKO]) mouse model with mice expressing the hepatic Igf-1 transgene (HIT) to generate the GHRKO-HIT mouse model. In GHRKO-HIT mice, serum IGF-1 levels were restored via transgenic expression of Igf-1, allowing us to study how endocrine IGF-1 affects growth, metabolic homeostasis, and skeletal integrity. We show that in a GH-resistant state, normalization of serum IGF-1 improved body adiposity and restored glucose tolerance but was insufficient to support normal skeletal growth, resulting in an osteopenic skeletal phenotype. The inability of serum IGF-1 to restore skeletal integrity in the total absence of GHR likely resulted from reduced skeletal Igf-1 gene expression, blunted GH-mediated effects on the skeleton that are independent of serum or tissue IGF-1, and poor delivery of IGF-1 to the tissues. These findings are consistent with clinical data showing that IGF-I replacement therapy in patients with Laron syndrome does not achieve full skeletal growth.Fil: Wu, Yingjie. University Of New York; Estados UnidosFil: Sun, Hui. University Of New York; Estados UnidosFil: Basta Pljakic, Jelena. City College of New York; Estados UnidosFil: Cardoso, Luis. City College of New York; Estados UnidosFil: Kennedy, Oran D.. City College of New York; Estados UnidosFil: Jasper, Hector Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Domene, Horacio Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Karabatas, Liliana Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Guida, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Schaffler, Mitchell B.. City College of New York; Estados UnidosFil: Rosen, Clifford J.. Maine Medical Center Research Institute; Estados UnidosFil: Yakar, Shoshana. University Of New York; Estados Unido

The Detection and Sequencing of a Broad-Host-Range Conjugative IncP-1 beta Plasmid in an Epidemic Strain of Mycobacterium abscessus subsp bolletii

Background: An extended outbreak of mycobacterial surgical infections occurred in Brazil during 2004-2008. Most infections were caused by a single strain of Mycobacterium abscessus subsp. bolletii, which was characterized by a specific rpoB sequevar and two highly similar pulsed-field gel electrophoresis (PFGE) patterns differentiated by the presence of a similar to 50 kb band. the nature of this band was investigated.Methodology/Principal Findings: Genomic sequencing of the prototype outbreak isolate INCQS 00594 using the SOLiD platform demonstrated the presence of a 56,264-bp circular plasmid, designated pMAB01. Identity matrices, genetic distances and phylogeny analyses indicated that pMAB01 belongs to the broad-host-range plasmid subgroup IncP-1 beta and is highly related to BRA100, pJP4, pAKD33 and pB10. the presence of pMAB01-derived sequences in 41 M. abscessus subsp. bolletii isolates was evaluated using PCR, PFGE and Southern blot hybridization. Sixteen of the 41 isolates showed the presence of the plasmid. the plasmid was visualized as a similar to 50-kb band using PFGE and Southern blot hybridization in 12 isolates. the remaining 25 isolates did not exhibit any evidence of this plasmid. the plasmid was successfully transferred to Escherichia coli by conjugation and transformation. Lateral transfer of pMAB01 to the high efficient plasmid transformation strain Mycobacterium smegmatis mc(2)155 could not be demonstrated.Conclusions/Significance: the occurrence of a broad-host-range IncP-1 beta plasmid in mycobacteria is reported for the first time. Thus, genetic exchange could result in the emergence of specific strains that might be better adapted to cause human disease.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundao de Amparo a Pesquisa do Estado de São PauloUniversidade Federal de São Paulo, Disciplina Microbiol, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, São Paulo, BrazilFed Univ Para, Inst Ciencias Biol, Lab Polimorfismo DNA, BR-66059 Belem, Para, BrazilInst Evandro Chagas, Secao Bacteriol & Micol, Ananindeua, PA, BrazilFed Univ Para, Inst Estudos Costeiros, Braganca, PA, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, Disciplina Microbiol, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, São Paulo, BrazilFAPESP: FAPESP - 06/01533-9Fundao de Amparo a Pesquisa do Estado de São Paulo: FAPESP - 8/01451-8Web of Scienc

Sunflower meal and supplementation of enzyme complex in diets for growing and finishing pigs

Aiming to evaluate the performance of pigs from 30 to 100 kg of live a total of 96 pigs with average live weight of 32.19±3.27 kg were distributed in randomized blocks design in a 4 × 2 (four levels of SM: 0, 8, 16 and 24%; with or without inclusion of EC)  factorial arrangement with six replicates and two animals per experimental unit. The parameters analyzed were feed intake (kg), weight gain (kg), feed conversion (kg/kg), backfat thickness (mm), carcass muscularity (kg), hot carcass weight (%), percentage of lean meat in the carcass (%), and quantity of lean meat in the carcass (kg). There was no interaction between EC and SM levels in the diet. The addition of EC in the diet affected the performance of the animals, but not carcass characteristics. Feed conversion of animals from 30 to 70 kg weight was improved by the inclusion of EC. The increasing levels of SM in the diet presented quadratic effect on weight gain and on backfat thickness, with inclusion points of SM that promoted maximum values of these parameters of 7.26% and 8.16%, respectively.

The Changing Landscape for Stroke\ua0Prevention in AF: Findings From the GLORIA-AF Registry Phase 2

Background GLORIA-AF (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients with Atrial Fibrillation) is a prospective, global registry program describing antithrombotic treatment patterns in patients with newly diagnosed nonvalvular atrial fibrillation at risk of stroke. Phase 2 began when dabigatran, the first non\u2013vitamin K antagonist oral anticoagulant (NOAC), became available. Objectives This study sought to describe phase 2 baseline data and compare these with the pre-NOAC era collected during phase 1. Methods During phase 2, 15,641 consenting patients were enrolled (November 2011 to December 2014); 15,092 were eligible. This pre-specified cross-sectional analysis describes eligible patients\u2019 baseline characteristics. Atrial fibrillation disease characteristics, medical outcomes, and concomitant diseases and medications were collected. Data were analyzed using descriptive statistics. Results Of the total patients, 45.5% were female; median age was 71 (interquartile range: 64, 78) years. Patients were from Europe (47.1%), North America (22.5%), Asia (20.3%), Latin America (6.0%), and the Middle East/Africa (4.0%). Most had high stroke risk (CHA2DS2-VASc [Congestive heart failure, Hypertension, Age  6575 years, Diabetes mellitus, previous Stroke, Vascular disease, Age 65 to 74 years, Sex category] score  652; 86.1%); 13.9% had moderate risk (CHA2DS2-VASc = 1). Overall, 79.9% received oral anticoagulants, of whom 47.6% received NOAC and 32.3% vitamin K antagonists (VKA); 12.1% received antiplatelet agents; 7.8% received no antithrombotic treatment. For comparison, the proportion of phase 1 patients (of N = 1,063 all eligible) prescribed VKA was 32.8%, acetylsalicylic acid 41.7%, and no therapy 20.2%. In Europe in phase 2, treatment with NOAC was more common than VKA (52.3% and 37.8%, respectively); 6.0% of patients received antiplatelet treatment; and 3.8% received no antithrombotic treatment. In North America, 52.1%, 26.2%, and 14.0% of patients received NOAC, VKA, and antiplatelet drugs, respectively; 7.5% received no antithrombotic treatment. NOAC use was less common in Asia (27.7%), where 27.5% of patients received VKA, 25.0% antiplatelet drugs, and 19.8% no antithrombotic treatment. Conclusions The baseline data from GLORIA-AF phase 2 demonstrate that in newly diagnosed nonvalvular atrial fibrillation patients, NOAC have been highly adopted into practice, becoming more frequently prescribed than VKA in Europe and North America. Worldwide, however, a large proportion of patients remain undertreated, particularly in Asia and North America. (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients With Atrial Fibrillation [GLORIA-AF]; NCT01468701

Omecamtiv mecarbil in chronic heart failure with reduced ejection fraction, GALACTIC‐HF: baseline characteristics and comparison with contemporary clinical trials

Aims: The safety and efficacy of the novel selective cardiac myosin activator, omecamtiv mecarbil, in patients with heart failure with reduced ejection fraction (HFrEF) is tested in the Global Approach to Lowering Adverse Cardiac outcomes Through Improving Contractility in Heart Failure (GALACTIC‐HF) trial. Here we describe the baseline characteristics of participants in GALACTIC‐HF and how these compare with other contemporary trials. Methods and Results: Adults with established HFrEF, New York Heart Association functional class (NYHA) ≥ II, EF ≤35%, elevated natriuretic peptides and either current hospitalization for HF or history of hospitalization/ emergency department visit for HF within a year were randomized to either placebo or omecamtiv mecarbil (pharmacokinetic‐guided dosing: 25, 37.5 or 50 mg bid). 8256 patients [male (79%), non‐white (22%), mean age 65 years] were enrolled with a mean EF 27%, ischemic etiology in 54%, NYHA II 53% and III/IV 47%, and median NT‐proBNP 1971 pg/mL. HF therapies at baseline were among the most effectively employed in contemporary HF trials. GALACTIC‐HF randomized patients representative of recent HF registries and trials with substantial numbers of patients also having characteristics understudied in previous trials including more from North America (n = 1386), enrolled as inpatients (n = 2084), systolic blood pressure < 100 mmHg (n = 1127), estimated glomerular filtration rate < 30 mL/min/1.73 m2 (n = 528), and treated with sacubitril‐valsartan at baseline (n = 1594). Conclusions: GALACTIC‐HF enrolled a well‐treated, high‐risk population from both inpatient and outpatient settings, which will provide a definitive evaluation of the efficacy and safety of this novel therapy, as well as informing its potential future implementation

Determinação dos valores energéticos de alguns óleos e gorduras para pintos de corte machos e fêmeas aos 21 dias de idade

Foi realizado um ensaio biológico com pintos de corte no período de 21 a 32 dias de idade, com o objetivo de determinar os valores de energia metabolizável aparente (EMA) e energia metabolizável aparente corrigida pela retenção de nitrogênio (EMAn) do óleo de soja degomado, óleo de milho, óleo de canola, gordura de aves, gordura de coco, gordura suína, sebo bovino e de duas misturas, sendo a primeira composta de óleo de soja degomado + gordura de coco e a segunda de óleo de soja degomado + sebo bovino, ambas na proporção de 1:1. Foi realizado o método de coleta total de excretas no experimento. O período de duração do experimento foi de dez dias, sendo os cinco primeiros de adaptação e os cinco restantes de coleta de excretas. Foi observada diferença entre os tratamentos e entre os sexos, sendo os valores de EMAn obtidos para fêmeas, em média, 98% dos valores de EMAn obtido para machos. Porém, não foi observada diferença entre a interação alimento x sexo. Os valores médios de EMAn encontrados para pintos de corte foram, respectivamente, para óleo de soja degomado, óleo de milho, óleo de canola, gordura de aves, gordura de coco, gordura suína, sebo bovino, para a mistura de óleo de soja degomado + gordura de coco e para a mistura de óleo de soja degomado + sebo bovino: 8.273, 8.582, 8.681, 8.511, 7.487, 7.536, 7.227, 8.029 e 8.171 kcal/kg matéria natura