Los derechos humanos: una reflexión desde la bioética

El presente artículo hace una revisión panorámica de la problemática de los derechos Humanos (DDHH) y del derecho Internacional Humanitario (DIH) como disciplinas en constante revisión y evolución. Se hace un recuento histórico de sus antecedentes a nivel universal y de Colombia. Se habla de la clasificación en una primera, segunda y tercera generación como una Carta de convivencia universal. Se recuerda los derechos omitidos. Se hace mención de las organizaciones gubernamentales y no gubernamentales a nivel nacional e internacional, se hace un recuerdo de la violación de los Derechos Humanos especialmente en USA y en Colombia

Nuevos tumores-nuevos desafíos

El presente artículo revisa los mas frecuentes tumores dediversos orígenes histológicos descritos en los últimosveinte años en la literatura. Se encontraron tumores deorigen epitelial, mesenquimal y otros cuyo origen aunno ha sido aclarado. Se describen los sitios depresentación, la clínica, al igual que los hallazgosanatomopatológicos y de inmunopatologí

Nuevos tumores-nuevos desafíos

El presente artículo revisa los mas frecuentes tumores dediversos orígenes histológicos descritos en los últimosveinte años en la literatura. Se encontraron tumores deorigen epitelial, mesenquimal y otros cuyo origen aunno ha sido aclarado. Se describen los sitios depresentación, la clínica, al igual que los hallazgosanatomopatológicos y de inmunopatologí

Spittlebugs: Bioecology, Host Plant Resistance and Advances in IPM

Several species and genera of spittlebugs (Homoptera: Cercopidae) are economic pests of grasses in tropical America. These insects compete with grazing animals by reducing forage availability and quality. They may cause serious losses on millions of hectares of improved pastures based on cultivars of several species of Brachiaria (signal grasses). Except for the cultivar Marandu, most of the available commercial cultivars of Brachiaria are susceptible to spittlebugs. In spite of their economic importance, much research need to be done yet. Such insect-plant system encompasses a diverse group of spittlebug species, a diverse group of forage grass species, which are under different management systems, in a broad range of ecological zones. Control efforts have been directed to host plant resistance, alternative that has been recognized as being of easy adoption and of low cost to farmers. It is probably the best control measure for controlling insect pests in low value crops, like pastures, widely established over vast areas. Screening for spittlebug resistance has been conducted both at CIAT and Embrapa-Beef Cattle Center, and promising accessions have been found. It is important however, that additional biological and behavioral studies of these insects, together with evaluations of other control techniques, like biological control and cultural practices, are also performed. Promising control measures and future research needs are discussed

Razlike u razinama anti-Müllerovog hormona u krvnoj plazmi i reproduktivnih parametara između dvije vrste goveda Bos indicus i Bubalus bubalis

The aim of this study was to evaluate the possible relationships between reproductive parameters and Anti Mullerian Hormone (AMH) levels in two closely related bovine species. AMH levels and the reproductive parameters of 50 water buffaloes (Bubalus bubalis) and 50 Zebu cattle (Bos indicus) of the same age and raised in similar conditions were evaluated. Clinical data were obtained from the farm in specialized designed format, and AMH was measured by ELISA. The results show that Zebu cattle had higher AMH levels than water buffaloes (P<0.0001), while water buffaloes exhibited better reproductive parameters: days open to pregnancy (P<0.0001), parity (P<0.0005), age at first calving (P<0.0001), and intercalving period (P<0.0001). Although both species have different reproductive parameters, no correlations were found between AMH and the reproductive parameters evaluated in either species. Finally, pregnant Zebu cattle and water buffalo showed a tendency to have lower AMH levels than non-pregnant counterparts. The results are paradoxical as the species with lower AMH levels exhibited better reproductive parameters, suggesting that the reproductive parameters within the herd do not depend solely on the ovarian reserve of females.Cilj je ovog rada bio procijeniti je li moguće ustanoviti povezanost između reproduktivnih parametara i razina anti-Müllerovog hormona (AMH) u dvije blisko srodne vrste goveda. Analizirane su razine AMH i reproduktivni parametri 50 bivola (Bubalus bubalis) i 50 zebu goveda (Bos indicus) iste dobi uzgojenih u sličnim uvjetima. Klinički podatci dobiveni su s farme u posebno osmišljenom pokusu. AMH je određivan pomoću ELISA metode. Rezultati pokazuju da su razine AMH više u goveda nego u bivola (P<0,0001). Bivoli su pokazali bolje reproduktivne parametre: servisno razdoblje (P<0,0001), paritet (P<0,0005), dob kod prvog teljenja (P<0,0001) i međutelidbeno razdoblje (P<0,0001). Premda obje vrste imaju različite reproduktivne parametre, nije ustanovljena povezanost između AMH i analiziranih reproduktivnih parametara niti u jedne od istraživanih vrsta. Zaključno, steone ženke goveda i bivola pokazuju tendenciju manjih razina AMH u usporedbi s nesteonim. Rezultati dobiveni u ovoj studiji su paradoksalni, jer vrste s nižim AMH razinama pokazuju bolje reproduktivne parametre. Ovi rezultati ukazuju na to da reproduktivni parametri unutar krda ne ovise samo o rezervi jajnih stanica u ženki

Effect of melatonin on follicular development parameters in a fixed-timed artificial insemination programme in water buffalo

It is well known that buffaloes have seasonal anoestrus and that the use of melatonin reduces the effects of season in reproduction, particularly among sheep and goats. This study evaluates the use of melatonin on water buffaloes to increase pregnancy rates in a fixed-time insemination programme. The study was conducted on an Argentinian buffalo herd, located in the Corrientes Province, during an unfavourable reproductive season. Artificial insemination cycles were performed in September and December 2018. Sixty animals were selected, and melatonin was administered to 29 females at a dose of 18 mg/50 kg. Ten days after the melatonin implant, ovulation synchronization was started. Animals were inseminated with frozen semen of a single bull. Pregnancy was evaluated by ultrasound 35 days after insemination. The pregnancy rate of the two repetitions was 34.62% for September and 6.4% for December (P=0.007). No significant differences were found in the parameters associated with follicular development and formation of the corpus luteum. Analysis of the effects of melatonin on the two replicates showed that there was no statistically significant effect of ovarian response regarding the analysed follicular development parameters. There was also no effect on pregnancy rates (17.85% vs. 20.68%; P=0.15), or on follicular development for treatment and control. In conclusion, this study was not able to demonstrate an effect of melatonin on the ovarian response of buffaloes as reported by other authors. Other factors, such as environmental conditions of subtropical areas and species specificities, may have a more significant effect on buffalo endocrinology. These factors should be evaluated to improve the results of fixedtimed artificial insemination programmes.Fil: Berdugo, Jesus Alfredo. Universidad Nacional de Colombia; ColombiaFil: Konrad, José Luis. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Yuponi, Roberto Gregorio. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Bandeo, Alexis Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Camelino, Ramon. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Cardona Maya, Walter D.. Universidad de Antioquia; ColombiaFil: Crudeli, Gustavo Angel. Universidad Nacional del Chaco Austral; Argentin

Proper cytoskeleton α‐tubulin distribution is concomitant to tyrosine phosphorylation during in vitro capacitation and acrosomal reaction in human spermatozoa

Spermatozoa motility is a key parameter during the fertilization process. In this context, spermatozoa tyrosine protein phosphorylation and an appropriate cytoskeleton α‐tubulin distribution are some of the most important physiological events involved in motility. However, the relationship between these two biomarkers remains poorly defined. Here, we characterized simultaneously by immunocytochemistry the α‐tubulin (TUBA4A) distribution and the tyrosine phosphorylation at flagellum before capacitation, during different capacitation times (1 and 4 hr), and after acrosome reaction induction in human spermatozoa. We found that the absence of spermatozoa phosphorylation in tyrosine residues positively and significantly correlated (p < 0.05) with the terminal piece α‐tubulin flagellar distribution in all physiological conditions. Conversely, we observed a positive significant correlation (p < 0.01) between phosphorylated spermatozoa and continuous α‐tubulin distribution in spermatozoa flagellum, independently of the physiological condition. Similarly, the subpopulation of spermatozoa with tyrosine phosphorylated and continuous α‐tubulin increases with longer capacitation times and after the acrosome reaction induction. Overall, these findings provide novel insights into the post‐transcriptional physiological events associated to α‐tubulin and the tyrosine phosphorylation during fertilization, which present potential implications for the improvement of spermatozoa selection methods.This research was supported by Human Fertility Cathedra of the University of Alicante, VIOGROB-186, and the project of the Ministry of Economy and Competitiveness AGL2015-70159-P

Social, Clinical and Microbiological Differential Characteristics of Tuberculosis among Immigrants in Spain

BACKGROUND: To identify the differential tuberculosis (TB) characteristics within the immigrant population with respect to natives in Spain. METHODOLOGY/PRINCIPAL FINDINGS: A prospective cohort study design was implemented to examine the TB cases diagnosed and starting standard antituberculous treatment in Spain, between January 1st 2006 and March 31st 2007. A logistic regression analysis was performed to determine differential characteristics. 1,490 patients were included in the study population, 1,048 natives and 442 (29.7%) immigrants. According to the multivariate analysis, the following variables were significantly associated with immigrant TB cases: younger age (OR = 3.79; CI:2.16-6.62), living in group situation (OR = 7.61; CI:3.38-12.12), lower frequency of disabled (OR:0.08; CI:0.02-0.26) and retired (OR:0.21; CI:0.09-0.48) employment status, lower frequency of pulmonary disease presentation (OR = 0.47; CI:0.24-0.92), primary or emergency care admission (OR = 1.80; CI:1.05-3.06 and OR = 2.16; CI:1.36-3.45), drug resistance (OR = 1.86; CI:1.01-3.46), treatment default (OR:2.12; CI:1.18-3.81), lower frequency of alcohol and cigarette consumption (OR = 2.10; CI:1.42-3.11 and OR = 2.85; CI:2.10-3.87 respectively), more directly observed treatment (OR = 1.68; CI:1.04-2.69), and poor understanding of TB disease and its treatment (OR = 3.11; CI:1.86-5.20). The low percentage of primary MDR-TB in the native population (0.1% vs. 2.2% of immigrants) should be noted. CONCLUSIONS/SIGNIFICANCE: The differences show the need to introduce specific strategies in the management of TB within the immigrant population, including the improvement of social and work conditions