Eutrophication and macroalgal blooms in temperate and tropical coastal waters: Nutrient enrichment experiments with Ulva spp.

Receiving coastal waters and estuaries are among the most nutrient-enriched environments on earth, and one of the symptoms of the resulting eutrophication is the proliferation of opportunistic, fast-growing marine seaweeds. Here, we used a widespread macroalga often involved in blooms, Ulva spp., to investigate how supply of nitrogen (N) and phosphorus (P), the two main potential growth-limiting nutrients, influence macroalgal growth in temperate and tropical coastal waters ranging from low- to high-nutrient supplies. We carried out N and P enrichment field experiments on Ulva spp. in seven coastal systems, with one of these systems represented by three different subestuaries, for a total of nine sites. We showed that rate of growth of Ulva spp. was directly correlated to annual dissolved inorganic nitrogen (DIN) concentrations, where growth increased with increasing DIN concentration. Internal N pools of macroalgal fronds were also linked to increased DIN supply, and algal growth rates were tightly coupled to these internal N pools. The increases in DIN appeared to be related to greater inputs of wastewater to these coastal waters as indicated by high δ15N signatures of the algae as DIN increased. N and P enrichment experiments showed that rate of macroalgal growth was controlled by supply of DIN where ambient DIN concentrations were low, and by P where DIN concentrations were higher, regardless of latitude or geographic setting. These results suggest that understanding the basis for macroalgal blooms, and management of these harmful phenomena, will require information as to nutrient sources, and actions to reduce supply of N and P in coastal waters concerned.Fil: Teichberg, Mirta. Leibniz Center For Tropical Marine Research; AlemaniaFil: Fox, Sophia E.. Marine Biological Laboratory; Estados UnidosFil: Olsen, Ylva S.. Bangor University; Reino UnidoFil: Valiela, Ivan. Marine Biological Laboratory; Estados UnidosFil: Martinetto, Paulina Maria del Rosario. Universidad Nacional de Mar del Plata; ArgentinaFil: Iribarne, Oscar Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Muto, Elizabeti Yuriko. Universidade de Sao Paulo; BrasilFil: Petti, Monica A.V.. Universidade de Sao Paulo; BrasilFil: Corbisier, Thaïs N.. Universidade de Sao Paulo; BrasilFil: Soto-Jiménez, Martín. Universidad Nacional Autónoma de México; MéxicoFil: Páez-Osuna, Federico. Universidad Nacional Autónoma de México; MéxicoFil: Castro, Paula. University Of Coimbra; BrasilFil: Freitas, Helena. University Of Coimbra; BrasilFil: Zitelli, Andreina. Università Iuav Di Venezia; ItaliaFil: Cardinaletti, Massimo. Gruppo Veritas; ItaliaFil: Tagliapietra, Davide. Consiglio Nazionale delle Ricerche; Itali

Cloning of sole proopiomelanocortin (POMC) cDNA and the effects of stocking density on POMC mRNA and growth rate in sole, Solea solea

Proopiomelanocortin (POMC) is an important gene implicated in different functions, such as the stress response of the hypothalamus–pituitary–adrenal axis. The aim of the present study was to determine whether farming conditions, such as stocking density, can be considered a powerful stressor influencing in turn the growth rate in juvenile fish. Thus, POMC cDNA expression was investigated during adaptation to farming conditions in sole (Solea solea), as a model for studying the effects of rearing densities on stress response; different stocking densities (50, 100, and 250 animals/m2) were applied and, after 7 and 21 days, the fishes were examined for body weight and plasma cortisol levels as indicators of stress. In addition, proopiomelanocortin was cloned and sequenced from the brain of sole, allowing semi-quantitative RT-PCR to be performed to evaluate POMC mRNA expression in brain tissue. There was a significant increase in cortisol levels in fish reared at high stocking densities of 250/m2 compared to fish reared at control densities of 100 and 50/m2, in both experimental times, i.e., 7 and 21 days. The high stocking densities were also found to decrease the specific growth rate of fish. Moreover, it was demonstrated that the highest stocking density induced a significant decrease in sole POMC mRNA expression. It is concluded that POMC and cortisol are both involved in the stress response due to high rearing densities, during which cortisol may serve as a negative regulator of POMC

Alpha2-adrenoreceptors profile modulation. 3.1 (R)-(+)-m-nitrobiphenyline, a new efficient and alpha2C-subtype selective agonist.

International audienceTo assess the stereochemical requirements for efficient alpha2C-adrenoreceptor activation, the enantiomeric forms of m-nitrobiphenyline [(+/-)-5] were prepared and tested on cells expressing the human alpha2-adrenoreceptor subtypes. The importance of chirality was confirmed, since the enantiomer (R)-(+)-5 was much more efficient than (S)-(-)-5 in producing alpha2C-activation. Surprising reversal of enantioselectivity was observed with respect to structurally similar biphenyline [(+/-)-1] whose (S)-(-)-form proved the preferred alpha2C-configuration

Annali del Dipartimento di Studi Letterari, Linguistici e Comparati. Sezione linguistica

"Annali del Dipartimento di Studi Letterari, Linguistici e Comparati. Sezione linguistica", ISSN 2281-6585, è una rivista internazionale a doppio cieco, peer-reviewed e open access pubblicata dall’Università degli Studi di Napoli “L’Orientale”. Classificazione della rivista: A (ANVUR) La Rivista si è occupata tradizionalmente delle lingue del mondo antico e accoglie anche contributi originali riferibili ad ambiti della linguistica sia teorica sia applicata. La Rivista valuta, inoltre, recensioni critiche, note e sommari