Devices and techniques for the characterization of inverse Compton sources

Innovative intense monochromatic x/ -ray sources are of great interest in the scientific community. A large number of applications in basic and applied physics research, as well as in different science fields, require an intense, monochromatic or quasi-monochromatic, tunable radiation source. Synchrotron radiation is optimal for low energy applications (< 100 keV) but the size and cost of synchrotron facilities prevent a large-scale spread of this kind of source, that is fundamental for applications such as routine clinical diagnostic. Moreover, synchrotron light is not suitable in the case of high energy applications (> 1 MeV), needed primarily for nuclear physics experiments, due to limitations on the maximum energy obtainable for monochromatic beams with synchrotron light. Alternative sources that can overcome such limitations are those based on inverse Compton interaction, which permit to obtain compact and cost-effective sources for low energy applications and can provide monochromatic collimated beam in the high energy range. Inverse Compton is the process in which a photon interacts with a relativistic electron, in this case the electron can transfer a fraction of its energy in the collision, resulting in a backscattered photon with an increased energy. This process can be used to produce hard x/ -rays by the backscattering of low-energy laser photons by a relativistic electron beam. A radiation source based on this interaction is usually called an inverse Compton source, alternatively, it can be called Thomson source when the energies involved allow a classical description of the process, as in the case of Thomson scattering. The work described in this dissertation concerns the devices and techniques developed to perform a characterization of inverse Compton sources. In particular, the work is focused on two major projects: BEATS2 experiment and ELI-NP-GBS proposal of E-Gammas collaboration. BEATS2 is an experiment funded by Istituto Nazionale di Fisica Nucleare (INFN) aimed to study medical applications, specially to mammographic imaging, of the SL-Thomson source of SPARC-LAB at the INFN-LNF that will be commissioned in the first half of 2013. E-Gammas is an international collaboration composed by several Universities and Institutions including: INFN and Universit`a di Roma La Sapienza, in Italy, Universitè de Paris Sud and IN2P3/CNRS, in France, and ASTeC of STFC, in UK. The collaboration is aimed to the preparation of a Technical Design Report for the ELI-NP Gamma Beam System (ELI-NP-GBS) to be commissioned by the end of 2016. This Gamma Beam System will be a high energy inverse Compton source, included in the Extreme Light Infrastructure - Nuclear physics (ELI-NP), an European project dedicated to the development of laser beams and the generation of high intensity gamma beams for frontier research in nuclear physics

Development of a new Front End electronics in Silicon and Silicon-Germanium technology for the Resistive Plate Chamber detector for high rate experiments

The upgrade of the Resistive Plate Chamber (RPC) detector, in order to increase the detector rate capability and to be able to work efficiently in high rate environment, consists in the reduction of the operating voltage along with the detection of signals which are few hundred {\mu}V small. The approach chosen by this project to achieve this objective is to develop a new kind of Front End electronics which, thanks to a mixed technology in Silicon and Silicon-Germanium, enhance the detector performances increasing its rate capability. The Front End developed is composed by a preamplifier in Silicon BJT technology with a very low inner noise (1000 $e^{-}$ rms) and an amplification factor of 0.3-0.4 mV/fC and a new kind of discriminator in SiGe HJT technology which allows a minimum threshold of the order of 0.5 mV. The performances of this kind of Front End will be shown. The results are obtained by using the CERN H8 beamline with a full-size RPC chamber of 1 mm gas gap and 1.2 mm thickness of electrodes equipped with this kind of Front End electronics.Comment: 14th Workshop on Resistive Plate Chambers and Related Detectors 19-23 February, 2018 Puerto Vallarta, Jalisco state, Mexic

Mechanistic insights into the release of doxorubicin from graphene oxide in cancer cells

Liposomal doxorubicin (L-DOX) is a popular drug formulation for the treatment of several cancer types (e.g., recurrent ovarian cancer, metastatic breast cancer, multiple myeloma, etc.), but poor nuclear internalization has hampered its clinical applicability so far. Therefore, novel drug-delivery nanosystems are actively researched in cancer chemotherapy. Here we demonstrate that DOX-loaded graphene oxide (GO), GO-DOX, exhibits much higher anticancer efficacy as compared to its L-DOX counterpart if administered to cellular models of breast cancer. Then, by a combination of live-cell confocal imaging and fluorescence lifetime imaging microscopy (FLIM), we suggest that GO-DOX may realize its superior performances by inducing massive intracellular DOX release (and its subsequent nuclear accumulation) upon binding to the cell plasma membrane. Reported results lay the foundation for future exploitation of these new adducts as high-performance nanochemotherapeutic agents

Generation of primary photons through inverse Compton scattering using a Monte Carlo simulation code

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Metabolomic Responses of Maize Shoots and Roots Elicited by Combinatorial Seed Treatments With Microbial and Non-microbial Biostimulants

Microbial and non-microbial plant biostimulants have been successfully used to improve agriculture productivity in a more sustainable manner. Since the mode of action of biostimulants is still largely unknown, the present work aimed at elucidating the morpho-physiological and metabolomic changes occurring in maize (Zea mays L.) leaves and roots following seed treatment with (i) a consortium of two beneficial fungi [arbuscular mycorrhizal fungi (AMF) and Trichoderma koningii TK7] and rhizobacteria, (ii) a protein hydrolyzate-based biostimulant (PH) alone, or (iii) in combination with a consortium of T. koningii TK7 and rhizobacteria. The application of PH alone or in combination with Trichoderma elicited significant increases (+16.6%) in the shoot biomass compared to untreated maize plants, whereas inoculation with AMF + Trichoderma elicited significant increases in root dry biomass (+48.0%) compared to untreated plants. Distinctive metabolomic signatures were achieved from the different treatments, hence suggesting that different molecular processes were involved in the plants response to the biostimulants. The metabolic reprogramming triggered by the treatments including the protein hydrolyzate was hierarchically more pronounced than the application of microorganisms alone. Most of the differential metabolites could be ascribed to the secondary metabolism, with phenylpropanoids and terpenes being the most represented compounds. The application of PH triggered an accumulation of secondary metabolites, whereas the opposite trend of accumulation was seen in the case of microorganisms alone. The increase in biomass could be related to two processes, namely the modulation of the multilayer phytohormone interaction network and a possible increase in nitrogen use efficiency via the GS-GOGAT system

A new method for spatial mode shifting of stabilized optical cavities for the generation of dual-color X-rays

We propose an innovative method to shift the transversal position of the focal point of an optical cavity keeping it actively frequency stabilized. Our cavity is a 4 mirrors bow-tie cavity and the spatial shift of the resonant mode is obtained by properly rotating the two curved mirrors by piezo actuators. This method allows us to move the transversal position of the cavity focal point of 135 µm in a time of 50 ms, keeping the resonance condition of the cavity by means of the Pound–Drever–Hall technique. We propose to use this technique for the generation of 2-color X-rays via Inverse Compton Scattering (ICS). This technique exploits the large average power stored in the high finesse cavity by shifting the laser beam with respect to the electron beam trajectory, hence controlling the spatial superposition of the electron and photon beams in the interaction region. Arranging two cavities assembled one on top of the other, with different collision angle with the electron beam, allows the generation of X-ray bursts of different energies just by swiftly moving the two cavities, switching the two focal points onto the electron beam trajectory, thus activating in sequence two different ICS spectral lines

Cadmium tolerance and phytochelatin content of Arabidopsis seedlings over-expressing the phytochelatin synthase gene AtPCS1

Previous studies demonstrated that expression of the Arabidopsis phytochelatin (PC) biosynthetic gene AtPCS1 in Nicotiana tabacum plants increases the Cd tolerance in the presence of exogenous glutathione (GSH). In this paper, the Cd tolerance of Arabidopsis plants over-expressing AtPCS1 (AtPCSox lines) has been analysed and the differences between Arabidopsis and tobacco are shown. Based on the analysis of seedling fresh weight, primary root length, and alterations in root anatomy, evidence is provided that, at relatively low Cd concentrations, the Cd tolerance of AtPCSox lines is lower than the wild type, while AtPCS1 over-expressing tobacco is more tolerant to Cd than the wild type. At higher Cd concentrations, Arabidopsis AtPCSox seedlings are more tolerant to Cd than the wild type, while tobacco AtPCS1 seedlings are as sensitive as the wild type. Exogenous GSH, in contrast to what was observed in tobacco, did not increase the Cd tolerance of AtPCSox lines. The PC content in wild-type Arabidopsis at low Cd concentrations is more than three times higher than in tobacco and substantial differences were also found in the PC chain lengths. These data indicate that the differences in Cd tolerance and in its dependence on exogenous GSH between Arabidopsis and tobacco are due to species-specific differences in the endogenous content of PCs and GSH and may be in the relative abundance of PCs of different length

Understanding the biostimulant action of vegetal-derived protein hydrolysates by high-throughput plant phenotyping and metabolomics: A case study on tomato

Designing and developing new biostimulants is a crucial process which requires an accurate testing of the product effects on the morpho-physiological traits of plants and a deep understanding of the mechanism of action of selected products. Product screening approaches using omics technologies have been found to be more efficient and cost effective in finding new biostimulant substances. A screening protocol based on the use of high-throughput phenotyping platform for screening new vegetal-derived protein hydrolysates (PHs) for biostimulant activity followed by a metabolomic analysis to elucidate the mechanism of the most active PHs has been applied on tomato crop. Eight PHs (A–G, I) derived from enzymatic hydrolysis of seed proteins of Leguminosae and Brassicaceae species were foliarly sprayed twice during the trial. A non-ionic surfactant Triton X-100 at 0.1% was also added to the solutions before spraying. A control treatment foliarly sprayed with distilled water containing 0.1% Triton X-100 was also included. Untreated and PH-treated tomato plants were monitored regularly using high-throughput non-invasive imaging technologies. The phenotyping approach we used is based on automated integrative analysis of photosynthetic performance, growth analysis, and color index analysis. The digital biomass of the plants sprayed with PH was generally increased. In particular, the relative growth rate and the growth performance were significantly improved by PHs A and I, respectively, compared to the untreated control plants. Kinetic chlorophyll fluorescence imaging did not allow to differentiate the photosynthetic performance of treated and untreated plants. Finally, MS-based untargeted metabolomics analysis was performed in order to characterize the functional mechanisms of selected PHs. The treatment modulated the multi-layer regulation process that involved the ethylene precursor and polyamines and affected the ROS-mediated signaling pathways. Although further investigation is needed to strengthen our findings, metabolomic data suggest that treated plants experienced a metabolic reprogramming following the application of the tested biostimulants. Nonetheless, our experimental data highlight the potential for combined use of high-throughput phenotyping and metabolomics to facilitate the screening of new substances with biostimulant properties and to provide a morpho-physiological and metabolomic gateway to the mechanisms underlying PHs action on plants

Fluorescence lifetime microscopy unveils the supramolecular organization of liposomal Doxorubicin

The supramolecular organization of Doxorubicin (DOX) within the standard Doxoves® liposomal formulation (DOX®) is investigated using visible light and phasor approach to fluorescence lifetime imaging (phasor-FLIM). First, the phasor-FLIM signature of DOX® is resolved into the contribution of three co-existing fluorescent species, each with its characteristic mono-exponential lifetime, namely: crystallized DOX (DOXc, 0.2 ns), free DOX (DOXf, 1.0 ns), and DOX bound to the liposomal membrane (DOXb, 4.5 ns). Then, the exact molar fractions of the three species are determined by combining phasor-FLIM with quantitative absorption/fluorescence spectroscopy on DOXc, DOXf, and DOXb pure standards. The final picture on DOX® comprises most of the drug in the crystallized form (∼98%), with the remaining fractions divided between free (∼1.4%) and membrane-bound drug (∼0.7%). Finally, phasor-FLIM in the presence of a DOX dynamic quencher allows us to suggest that DOXf is both encapsulated and non-encapsulated, and that DOXb is present on both liposome-membrane leaflets. We argue that the present experimental protocol can be applied to the investigation of the supramolecular organization of encapsulated luminescent drugs/molecules all the way from the production phase to their state within living matter