Contribution to phaneroptical study in andalusian hound dog breed

We have studied the type of hair and the coat colour of the Andalusian Hound breed with a view to characterise and typify the nine varieties recognised in the racial pattern. We have concluded the existence of highly significant differences between sizes for the variables type of hair and coat colour, what it is confirming the present reproductive separation and isolation of each variety in the management of the breed book. At the same time we have developed a study of the variable related to the mouth form obtaining the majority of the animal presenting correct mouth caught, but the defect prognatism appear in all the varieties, while the enogmatism only appear in the medium size varieties.Se estudia el tipo de pelo y la coloración de la capa dentro de la raza Podenco Andaluz con vistas a conseguir la caracterización y tipificación de las nueve variedades reconocidas en su patrón racial. Se concluye la existencia de diferencias altamente significativas entre la variable talla y las variables tipo de pelo y color de la capa lo que confirma el actual tratamiento en la separación y aislamiento reproductivo en cada una de las variedades dentro de la gestión de los registros genealógicos de la raza. Asimismo se realiza un estudio de las variables fanerópticas de la boca obteniendo una mayoría de animales que presentan un tipo de mordidas correctas si bien el defecto de mordida prognata está presente en todas las variedades mientras que el enogmatismo sólo aparece en las correspondientes a la talla media

Voltage-dependent Dynamic FRET Signals from the Transverse Tubules in Mammalian Skeletal Muscle Fibers

Two hybrid voltage-sensing systems based on fluorescence resonance energy transfer (FRET) were used to record membrane potential changes in the transverse tubular system (TTS) and surface membranes of adult mice skeletal muscle fibers. Farnesylated EGFP or ECFP (EGFP-F and ECFP-F) were used as immobile FRET donors, and either non-fluorescent (dipicrylamine [DPA]) or fluorescent (oxonol dye DiBAC4(5)) lipophilic anions were used as mobile energy acceptors. Flexor digitorum brevis (FDB) muscles were transfected by in vivo electroporation with pEGFP-F and pECFP-F. Farnesylated fluorescent proteins were efficiently expressed in the TTS and surface membranes. Voltage-dependent optical signals resulting from resonance energy transfer from fluorescent proteins to DPA were named QRET transients, to distinguish them from FRET transients recorded using DiBAC4(5). The peak ΔF/F of QRET transients elicited by action potential stimulation is twice larger in fibers expressing ECFP-F as those with EGFP-F (7.1% vs. 3.6%). These data provide a unique experimental demonstration of the importance of the spectral overlap in FRET. The voltage sensitivity of QRET and FRET signals was demonstrated to correspond to the voltage-dependent translocation of the charged acceptors, which manifest as nonlinear components in current records. For DPA, both electrical and QRET data were predicted by radial cable model simulations in which the maximal time constant of charge translocation was 0.6 ms. FRET signals recorded in response to action potentials in fibers stained with DiBAC4(5) exhibit ΔF/F amplitudes as large as 28%, but their rising phase was slower than those of QRET signals. Model simulations require a time constant for charge translocation of 1.6 ms in order to predict current and FRET data. Our results provide the basis for the potential use of lipophilic ions as tools to test for fast voltage-dependent conformational changes of membrane proteins in the TTS

Pulmonary Artery Dissection Post-blunt Thoracoabdominal Trauma

Background: Pulmonary artery dissection is a rare condition that is usually diagnosed in patients exhibiting chronic pulmonary arterial hypertension, congenital heart abnormalities or secondary to iatrogenic injury. Diagnosis is often made at autopsy as many patients experience sudden death when the pulmonary artery dissection progresses rapidly and ruptures into the pericardium, resulting in acute cardiac tamponade.Case Presentation: We report a case of pulmonary artery dissection, which resulted from blunt thoracic trauma diagnosed in the emergency department

Quantitative analysis of protein glycation in clinical samples

Comunicaciones a congreso

Description of analyzing power and (p,n) reaction by a global dispersive coupled-channel optical model potential

A global dispersive coupled-channel optical model potential is used to analyze the proton elastic/inelastic analyzing power data and (p,n) data in order to investigate the prediction power of this potential. The numerical calculations show good agreement with available experimental data for proton elastic data and overall agreement for proton inelastic data. The results also showed that present global potential is approximately Lane-consistent, and can predict well the (p,n) cross sections

Perfil fenólico de aceites de oliva vírgenes obtenidos de selecciones avanzadas en un programa de mejora

The evaluation of the phenolic composition in advanced selections in breeding programs constitutes the first approach for selecting genotypes with improved olive oil quality. In this work, the influence of genotype and ripening index on the phenolic profile of olive oils from advanced selections in comparison to their genitors was studied. Fruit samples were collected in genotypes from crosses between ‘Arbequina’ × ‘Picual’, ‘Picual’ × ‘Arbequina’ and ‘Frantoio’ × ‘Picual’ at five dates from 1st October to 26th November 2009. Characterization of the phenolic profile was performed by liquid–liquid extraction with 60:40 (v/v) methanol–water and subsequent chromatographic analysis with absorption and fluorescence detection in a sequential configuration. A dual effect of genotype and fruit ripening on the phenolic profile has been observed with more pronounced genetic influence in both total (34.73% and 20.45%, respectively) and individual phenols (16.99% to 49.25% and 1.58% to 23.77%, respectively). A higher degree of variability between genotypes at early ripening stages was also observed (p<0.05). The obtained results also allow identification of selections with high content of total and individual phenols. These results suggest a strategy based on early harvesting of fruits (at the first three ripening indexes) for better comparison and selection of genotypes in further crosses in olive breeding programs aiming at improving the quality of virgin olive oil.La evaluación de la composición fenolica en selecciones avanzadas en programas de mejora constituye el primer paso para la selección de genotipos cuyos aceites son de mejor calidad. Se ha estudiado la influencia del genotipo y del índice de madurez en el perfil fenólico del aceite de oliva de selecciones avanzadas en comparación con sus genitores. Se recogieron muestras de genotipos obtenidos de cruzamientos entre 'Arbequina' × 'Picual', 'Picual' × 'Arbequina' y 'Frantoio' × 'Picual' en cinco fechas entre el 1 de octubre y el 26 noviembre de 2009. Se realizó la caracterización del perfil fenólico por extracción líquido–líquido con metanol–agua al 60:40 (v/v) seguido de un análisis cromatográfico con detección por absorción y fluorescencia en una configuración secuencial. Los resultados obtenidos mostraron un mayor grado de variabilidad entre genotipos en las primeras etapas de maduración de los frutos (p<0.05), así como el efecto de la madurez de los frutos y del genotipo en el perfil fenólico, con una influencia genética más pronunciada tanto para fenoles totales (34.73% and 20.45%, respectivamente) como individuales (entre 16.99% y 49.25% y entre 1.58% y 23.77%, respectivamente). Los resultados obtenidos han permitido la identificación de selecciones con alto contenido en fenoles totales e individuales. Ambos resultados sugieren una estrategia basada en la evaluación de los fenoles totales e individuales en las primeras etapas de la maduración para comparar y seleccionar genotipos en programas de mejora por cruzamiento encaminados a mejorar la calidad del aceite de oliva virgen

Behaviour of the descendent of cuban creole pigs

The present work was proposed to study the behaviour of the Cuban’s pig descendent derived of 27 sow and 5 boar and yours descendent from 93 calving. The animals were exploration by intensive system with lower quality supplements and sweet by sugarcane. The reproductive method utilised was the assisted mount. The results from piglets was: 7.52 total piglet was born; 6.77 piglets was born live; 6.42 weaning piglets; 0.912 kg means of the weight piglets was born live and 6.42 means of piglets weaning. The means of the Cubans Pigs observed in this study was similar with others Cuban and Spanish authors that study this kind of animals. There are differences between the Cubans Pigs and Iberians Pigs, were the Iberians Pigs showed the best weight was born.En este trabajo se estudió el comportamiento de las descendencias de un rebaño de cerdos Criollos Cubanos en pureza, formado por 27 cerdas y 5 sementales y sus descendencias procedentes de 93 partos, explotados en un sistema de crianza estabulado y con un régimen de alimentación basándose en pienso B de baja calidad y suplementados con mieles de caña de azúcar. El método reproductivo empleado fue la monta dirigida. Los resultados del comportamiento de algunos indicadores de camada fueron los siguientes: total de crías nacidas, 7,52; crías nacidas vivas, 6,77; crías destetadas, 6,42; peso promedio de las crías al nacer 0,912 kg y peso promedio de las crías al destete, 6,03 kg. En cuanto al comportamiento medio de las crías nacidas vivas y destetadas se corresponde con reportes de otros autores cubanos que estudian el Cerdo Criollo Cubano y con autores españoles respecto a las variedades del Ibérico. Las diferencias con los animales de este tronco se aprecian al comparar los pesos al nacimiento y a los 30 días de edad, donde el Criollo Cubano se ve superado por las distintas variedades de animales que le dieron origen

A K=3 two-quasiparticle isomer in 98^{98}Sr

The decay of on-line mass-separated 98 Rb to 98 Sr is studied by γ spectroscopy. The revised decay scheme adds further evidence of the coexistence of very different shapes in 98 Sr . A set of levels is proposed to originate from particle-hole pair excitations across the Z = 40 spherical gap in analogy with 96 Sr . A deformed K = 3 band with probable even parity is built on a 7.1-ns isomer at 1838 keV. It is interpreted as a two-quasineutron excitation in accordance with a quantum Monte Carlo pairing calculation based on a deformed shell model. Configurations of the calculated lowest-lying two-quasiparticle levels confirm the importance of the [404]9/2 neutron orbital at the largest deformations in the neutron-rich A ≃ 100 region

Microsatellite variation in Spanish Alano dogs

We have studied a sample of 35 spanish alano dogs in order to estimate the level of genetic variability that can be detected by using microsatellites and the efficiency of these genetic markers as tools for parentage testing in this canine population. We have used 4 microsatellites previously described in the literature. All of them were polymorphic, with an average number of 5 alleles/ locus and an average gene diversity (H) of 0,70 ± 0,06, similar to the figures reported in others breeds. Two of the microsatellites showed disa-greement with Hardy-Weinberg proportions, due to a deficit of heterozygotes. We have found in one of the microsatellites alleles that could be breed specific, as they have not been detected in other spanish breeds. The combined exclusion probability detected is higher than 90 percent, suggesting the possibility of using efficiently microsatellites in parentage testing in the spanish alano population.En una muestra de 35 alanos españoles se investigó la utilidad de los microsatélites para detectar variabilidad genética y para el control de paternidad. Los 4 microsatélites utilizados, descritos ya en la bibliografía, resultaron polimórficos, con un número medio de 5 alelos/ locus y una heterocigosis media (H), de 0,70 ± 0,06, similar a la de otras razas caninas. Se ha detectado una desviación del equilibrio Hardy-Weinberg para dos microsatélites debida a un déficit de heterocigotos. En un microsatélite se han detectado alelos inexistentes en otras razas españolas, por lo que podrían ser específicos de raza. La probabilidad acumulada de exclusión de paternidad es superior al 90 p.100, por lo que podrían ser eficaces para estas pruebas en alanos

Experimental uncertainty and covariance information in EXFOR library

Compilation of experimental uncertainty and covariance information in the EXFOR Library is discussed. Following the presentation of a brief history of information provided in the EXFOR Library, the current EXFOR Formats and their limitations are reviewed. Proposed extensions for neutron-induced reaction cross sections in the fast neutron region and resonance region are also presented.JRC.D.4-Standards for Nuclear Safety, Security and Safeguard