Paglia on Taylor: "A luscious, opulent, ripe fruit!"

When news broke that Elizabeth Taylor had died at 79, we immediately reached out to founding Salon contributor and lifelong Taylor obsessive Camille Paglia for her thoughts. We found her in a Philadelphia research library researching her new visual arts book for Pantheon, but she diligently trekked outside in the rain to speak to Salon editor-in-chief Kerry Lauerman by telephone under a portico, as the wind howled around her

Dendritic Cells Crosspresent Antigens from Live B16 Cells More Efficiently than from Apoptotic Cells and Protect from Melanoma in a Therapeutic Model

Dendritic cells (DC) are able to elicit anti-tumoral CD8+ T cell responses by cross-presenting exogenous antigens in association with major histocompatibility complex (MHC) class I molecules. Therefore they are crucial actors in cell-based cancer immunotherapy. Although apoptotic cells are usually considered to be the best source of antigens, live cells are also able to provide antigens for cross-presentation by DC. We have recently shown that prophylactic immunotherapy by DC after capture of antigens from live B16 melanoma cells induced strong CD8+ T-cell responses and protection against a lethal tumor challenge in vivo in C57Bl/6 mice. Here, we showed that DC cross-presenting antigens from live B16 cells can also inhibit melanoma lung dissemination in a therapeutic protocol in mice. DC were first incubated with live tumor cells for antigen uptake and processing, then purified and irradiated for safety prior to injection. This treatment induced stronger tumor-specific CD8+ T-cell responses than treatment by DC cross-presenting antigens from apoptotic cells. Apoptotic B16 cells induced more IL-10 secretion by DC than live B16 cells. They underwent strong native antigen degradation and led to the expression of fewer MHC class I/epitope complexes on the surface of DC than live cells. Therefore, the possibility to use live cells as sources of tumor antigens must be taken into account to improve the efficiency of cancer immunotherapy

Lecture by Camille Paglia, given at Florida International University, Sept.15, 2006

Camille Paglia discusses the exhibit A Room of One\u27s Own . Introduction by Dahlia Morgan

Paglia on Taylor: "A luscious, opulent, ripe fruit!"

When news broke that Elizabeth Taylor had died at 79, we immediately reached out to founding Salon contributor and lifelong Taylor obsessive Camille Paglia for her thoughts. We found her in a Philadelphia research library researching her new visual arts book for Pantheon, but she diligently trekked outside in the rain to speak to Salon editor-in-chief Kerry Lauerman by telephone under a portico, as the wind howled around her