Comau metagenomic reads, functional annotation

Functional annotation of the metagenomic sequences of a microbial mat from the Comau Fjord, Chile. The annotation was done using CAMERA, with the RAMMCAP pipelin

Comau metagenomic reads, taxonomy annotation

Taxonomic annotation of the sequence reads obtained from a microbial mat in the Comau Fjord, Chile. The reads were classified using MG-TAXA

Taxonomic classification of KEGG pathways related to energy metabolism.

<p>Each pathway was determined to be present in the community based on the MinPath analysis [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071952#B18" target="_blank">18</a>]. Taxonomic affiliation of each sequence was based on the results provided by the IMG-MER annotation [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071952#B9" target="_blank">9</a>].</p

Microbial Life in a Fjord: Metagenomic Analysis of a Microbial Mat in Chilean Patagonia

<div><p>The current study describes the taxonomic and functional composition of metagenomic sequences obtained from a filamentous microbial mat isolated from the Comau fjord, located in the northernmost part of the Chilean Patagonia. The taxonomic composition of the microbial community showed a high proportion of members of the <i>Gammaproteobacteria</i>, including a high number of sequences that were recruited to the genomes of <i>Moritella marina</i> MP-1 and <i>Colwellia</i><i>psycherythraea</i> 34H, suggesting the presence of populations related to these two psychrophilic bacterial species. Functional analysis of the community indicated a high proportion of genes coding for the transport and metabolism of amino acids, as well as in energy production. Among the energy production functions, we found protein-coding genes for sulfate and nitrate reduction, both processes associated with <i>Gammaproteobacteria</i>-related sequences. This report provides the first examination of the taxonomic composition and genetic diversity associated with these conspicuous microbial mat communities and provides a framework for future microbial studies in the Comau fjord.</p> </div

Recruitment of metagenomic reads against the genomes of <i>C</i><i>. psycherythraea</i> and <i>M. marina</i>.

<p>Genome is colored according to COG categories, and blue lines indicate reads from the metagenome that were recruited to the reference genome at ≥ 80% identity.</p

Patches of white cotton-like microbial mats on the rocky walls of the Comau fjord.

<p>(Photo by Verena Häussermann and Günter Försterra).</p

Odds ratio of COG categories of all sequenced bacterial genomes versus the Comau metagenome.

<p>Values over 0 indicate categories that are enriched in the bacterial genomes versus the Comau metagenome, and values less than 0 indicate categories where the Comau metagenome is enriched versus all the bacterial genomes. With the exception of the cell cycle category, all other groups showed significant changes by two-tailed Fisher exact test, at 95% confidence level.</p