Entropic Characterization of Mixing in Microchannels

A methodology for rigorous mixing assessment in microchannels is presented. The analysis is based on numerical simulations of flow in different geometries coupled with mixing assessment using entropic measures. The results show enhanced mixing efficiency for the staggered herringbone micromixer by comparison with a mixer with straight diagonal ridges and a lack of mixing in a non-patterned channel. These results are in agreement with published experimental data

Quantifying Fluid Mixing with The Shannon Entropy

We introduce a methodology to quantify the quality of mixing in various systems, including polymeric ones, by adapting the Shannon information entropy. For illustrative purposes we use particle advection of two species in a two-dimensional cavity flow. We compute the entropy by using the probability of finding a suitable chosen group/complex of particles of a given species, at a given location. By choosing the size of the group to be in direct proportion to the overall concentration of the components in the mixture we ensure that the entropic measure is maximized for the case of perfect mixing, that is, when at each location the component concentration is equal to the corresponding overall component concentrations. The scale of observation role in evaluating mixing is analyzed using the entropic methodology. We also illustrate the effect of initial conditions on mixing in a laminar system, typical in operations involving polymers

Genome Sequence of the Native Apiculate Wine Yeast Hanseniaspora vineae T02/19AF

The use of novel yeast strains for winemaking improves quality and provides variety including subtle characteristic differences in fine wines. Here we report the first genome of a yeast strain native to Uruguay, Hanseniaspora vineae T02/19AF, which has been shown to positively contribute to aroma and wine quality.Fil: Giorello, Facundo M.. Universidad de la República; UruguayFil: Berná, Luisa. Instituto Pasteur de Montevideo; UruguayFil: Greif, Gonzalo. Instituto Pasteur de Montevideo; UruguayFil: Camesasca, Laura. Inst. de Investigaciones Biológicas Clemente Estable; UruguayFil: Salzman, Valentina. Instituto Pasteur de Montevideo; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Medina, Karina. Universidad de la Republica. Facultad de Química; UruguayFil: Robello, Carlos. Instituto Pasteur de Montevideo; UruguayFil: Gaggero, Carina. Inst. de Investigaciones Biológicas Clemente Estable; UruguayFil: Aguilar, Pablo S.. Instituto Pasteur de Montevideo; UruguayFil: Carrau, Francisco. Sección Enología; Urugua

The Effect of Stocking Rate and Lamb Grazing System on Sward Performance of \u3cem\u3eTrifolium Repens\u3c/em\u3e and \u3cem\u3eLotus Corniculatus\u3c/em\u3e in Uruguay

Lambs have a great potential to diversify and stimulate meat and wool production and economical returns within the industry. The main objective of this study was to evaluate different feeding and management alternatives for the production of high quality wool and meat as well as their effects on sward characteristics in the Basaltic region of Uruguay

Numerical and experimental study of agglomerate dispersion in polymer extrusion

A model for agglomerate dispersion in screw extruders was developed and superimposed on the flow patterns as simulated using the FIDAP software. A particle tracking algorithm with an adaptive time step was used to follow the agglomerates trajectory. Along this flow path, the breakup probability was estimated using a Monte Carlo method and in conjunction with the local fragmentation number. Particle size distributions and Shannon entropy were computed along the screw channel. The results show good qualitative agreement between model predictions and experimental data

Global mixing indices for single screw extrusion

The present work presents numerical simulations of dispersive and distributive mixing of a two phase system being processed in a single screw extruder under various conditions. Models were developed to quantify the degree of mixing of liquid-liquid and solids-liquid systems, for a given set of material properties, operating conditions and extruder geometry

High-density perfusion cultures of the marine bacterium Rhodovulum sulfidophilum for the biomanufacturing of oligonucleotides

Therapeutic oligonucleotides (ONs) are typically manufactured via solid-phase synthesis, characterized by limited scalability and huge environmental footprint, limiting their availability. Biomanufactured ONs have the potential to reduce the immunogenic side-effects, and to improve the sustainability of their chemical counterparts. Rhodovulum sulfidophilum was demonstrated a valuable host for the extracellular production of recombinant ONs. However, low viable cell densities and product titer were reported so far. In this work, perfusion cell cultures were established for the intensification of ON biomanufacturing. First, the perfusion conditions were simulated in 50 mL spin tubes, selected as a scale-down model of the process, with the aim of optimizing the medium composition and process parameters. This optimization stage led to an increase in the cell density by 44 % compared to the reference medium formulation. In addition, tests at increasing perfusion rates were conducted until achieving the maximum viable cell density (VCDmax), allowing the determination of the minimum cell-specific perfusion rate (CSPRmin) required to sustain the cell culture. Intriguingly, we discovered in this system also a maximum CSPR, above which growth inhibition starts. By leveraging this process optimization, we show for the first time the conduction of perfusion cultures of R. sulfidophilum in bench-scale bioreactors. This process development pipeline allowed stable cultures for more than 20 days and the continuous biomanufacturing of ONs, testifying the great potential of perfusion processes

Implementation of the Damages Directive in England & Wales

The Dossier discusses the questions arising from the need to implement the EU Damages Directive 2014/104/EU in several European Member States. My contribution focuses on the need for implementation in England & Wales

Determinants of the duration of European appellate court proceedings in cartel cases

The duration of appellate court proceedings is an important determinant of the efficiency of a court system. We use data of 234 firm groups that participated in 63 cartels convicted by the European Commission between 2000 and 2012 to investigate the determinants of the duration of the subsequent one- or two-stage appeals process. We find that while the speed of the firststage appellate court decision depends on the court’s appeals-related workload, the complexity of the case, the degree of cooperation by the firms involved and the clarity of the applied rules and regulations, the second-stage appellate court proceedings appear to be largely unaffected by those drivers. We take our empirical results to derive conclusions for both firms that plan to file an appeal as well as public policy makers