56 research outputs found
Transient dsDNA breaks during pre-replication complex assembly
Get PDFInitiation of DNA replication involves the ordered assembly of the multi-protein pre-replicative complex (pre-RC) during G1 phase. Previously, DNA topoisomerase II (topo II) was shown to associate with the DNA replication origin located in the lamin B2 gene locus in a cell-cycle-modulated manner. Here we report that activation of both the early-firing lamin B2 and the late-firing hOrs8 human replication origins involves DNA topo II-dependent, transient, site-specific dsDNA-break formation. Topo IIβ in complex with the DNA repair protein Ku associates in vivo and in vitro with the pre-RC region, introducing dsDNA breaks in a biphasic manner, during early and mid-G1 phase. Inhibition of topo II activity interferes with the pre-RC assembly resulting in prolonged G1 phase. The data mechanistically link DNA topo IIβ-dependent dsDNA breaks and the components of the DNA repair machinery with the initiation of DNA replication and suggest an important role for DNA topology in origin activation
The repressor which binds the -75 GATA motif of the GPB promoter contains Ku70 as the DNA binding subunit
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The E-Box of the Human Glycophorin B Promoter Is Involved in the Erythroid-Specific Expression of the GPB Gene
No full textTranscriptional regulation of the KEL gene and Kell protein expression in erythroid and non-erythroid cells
No full textTranscriptional regulation of the KEL gene and Kell protein expression in erythroid and non-erythroid cells
No full textNext-generation sequencing: proof of concept for antenatal prediction of the fetal Kell blood group phenotype from cell-free fetal DNA in maternal plasma
No full textPan-HDAC Inhibitors May Restore PRDM1 Expression in Follicular Lymphoma
No full textInternational audienc
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