Cell localization of southern bean mosaic virus proteins in cowpea

The southern bean mosaic virus (SBMV), the type member of the sobemovirus genus, is a positive sense icosohedral plant virus that systemically infects cowpea. Recently, it was determined that the p21 (ORF1) protein, p24 (ORF3) protein, and the CP were required for cell-to-cell movement of SBMV-C in cowpea. It is possible that these proteins are viral movement proteins, that is, they provide some function in viral movement. The goal of this study was to determine the subcellular localization of these three viral proteins and begin to elucidate their roles in cell-to-cell and/or long distance movement. Previously, the movement characteristics of this virus were unknown. Using immunocytochemistry, virus was found to localize in the xylem and phloem of infected cowpea, although most plant viruses traffic only in the phloem. Cell-to-cell movement of plant viruses is believed to occur by one of two methods. One possibility (i.e. tobacco mosaic virus) is through the alteration of the plant\u27s plasmodesmata. The second method (i.e. cowpea mosaic virus) uses cell wallspanning tubules. Using immunocytochemistry, localization of ORF1, ORF3, and the CP was performed. The ORF1 protein was found to localize near plasmodesmata, indicating that this viral protein is involved in cell-to-cell movement. There was no evidence of tubule formation. The ORF3 protein did not localize to any specific areas within a cell, although higher concentrations of this protein were found in the xylem vascular cells. The SBMV-C CP was also found to localize in plasmodesmata, indicating a possible role in cell-to-cell movement of SBMV-C. Based on the results of this study, it is proposed that SBMV-C traffics as viral particles, but does not move cell-to-cell via tubules. Instead, the vims moves through the plant\u27s plasmodesmata through the activity of the ORF1 protein and possibly the CP. The long distance movement characteristics of SBMV-C were also studied. The accumulation of the ORF1 and CP in the secondary cell walls of the sieve elements suggests a role for these proteins in systemic movement of SBMV-C

Agriculture, Trade, and Development in the International Political Economy: A Case Study of Jamaica

This project hypothesizes that Jamaica should be more developed given its natural and human resource endowment. In light of Jamaica's agricultural underdevelopment, the project utilizes an agricultural framework to assess the processes by which politico-economic forces have shaped development in Jamaica and the larger Caribbean since independence. The project conjectures that both externally-driven and domestically-motivated forces have impeded Jamaica's development and investigates the extent to which these forces have forestalled national development. In so doing, the research tests the validity of the following competing theories of development for Jamaica: neoliberalism and dependencia. The Jamaican experience is highlighted as a case study that is representative of and generalizable to the Caribbean at large. As the Caribbean country that is arguably the most structurally adjusted in the region, with significant ties to the United States, the Jamaican experience can be viewed in the (dominant) neoliberal paradigm as the "best case scenario" for development in the region, one that should have "made it." As Elsie Le Franc (1994) stated, "one can always identify 'winners' and 'losers' in any situation of change, but it is necessary to try to tackle that more difficult issue of whether or not any identifiable winners can function, and most important, expand in a market economy." The research therefore demonstrates how structural adjustment conditionalities; bilateral, regional, and multilateral trade realities; limited investment into local agriculture; and overall domestic apathy and lack of agricultural reform have played out in what has been argued to be a model case for socioeconomic expansion, and how they have shaped that country's development and options in our rapidly globalizing market economy. The project's research findings reveal the conception of development, neoliberal or dependency, that is more relevant to Jamaica's experience. Finally, recognizing that mainstream strategies of development have not eradicated the problems of Caribbean underdevelopment, the project proposes an alternate model of development that reflects the voice of all segments of society and has at its core a strong state that fosters technological innovation, encourages export diversification, and channels investment to improve Jamaica's production, productivity, and competitiveness in the international political economy

Shale oil : potential economies of large-scale production, preliminary phase

Producing shale oil on a large scale is one of the possible alternatives for reducing dependence of the United States on imported petroleum. Industry is not producing shale oil on a commercial scale now because costs are too high even though industry dissatisfaction is most frequently expressed about "non-economic" barriers: innumerable permits, changing environmental regulations, lease limitations, water rights conflicts, legal challenges, and so on. The overall purpose of this study is to estimate whether improved technology might significantly reduce unit costs for production of shale oil in a planned large-scale industry as contrasted to the case usually contemplated: a small industry evolving slowly on a project-by-project basis. In this preliminary phase of the study, we collected published data on the costs of present shale oil technology and adjusted them to common conditions; these data were assembled to help identify the best targets for cost reduction through improved large-scale technology They show that the total cost of producing upgraded shale oil (i.e. shale oil accpetable as a feed to a petroleum refinery) by surface retorting ranges from about $18 to$28/barrel in late '78 dollars with a 20% chance that the costs would be lower than and 20% higher than that range. The probability distribution reflects our assumptions about ranges of shale richness, process performance, rate of return, and other factors that seem likely in a total industry portfolio of projects. About 40% of the total median cost is attributable to retorting, 20% to upgrading, and the remaining 40% to resource acquisition, mining, crushing, and spent shale disposal and revegetation. Capital charges account for about 70% of the median total cost and operating costs for the other 30%. There is a reasonable chance that modified in-situ processes (like Occidental's) may be able to produce shale oil more cheaply than surface retorting, but no reliable cost data have been published; in 1978, DOE estimated a saving of roughly $5/B for in-situ. Because the total costs of shale oil are spread over many steps in the production process, improvements in most or all of those steps are required if we seek a significant reduction in total cost. A June 1979 workshop of industry experts was held to help us identify possible cost-reduction technologies. Examples of the improved large-scale technologies proposed (for further evaluation) to the workshop were: - Instead of hydrotreating raw shale oil to make syncrude capable of being refined conventionally, rebalance all of a refinery's processes (or develop new catalysts/processes less sensitive to feed nitrogen) to accommodate shale oil feed -- a change analogous to a shift from sweet crude to sour crude. - Instead of refining at or near the retort site, use heated pipelines to move raw shale oil to existing major refining areas. - Instead of operating individual mines, open-pit mine all or much of the Piceance Creek Basin. - Instead of building individual retorts, develop new methods for mass production of hundreds of retorts

Cosmopolitan nationalism and the cultural reach of the white British

In recent years, strong claims have been made for the breakdown of national boundaries and the reformation of national identities in an increasingly interconnected global world – driven in large part by the possibilities and limitations that emerge from an increasingly global media world. It has been argued that new postnational, cosmopolitan subjectivities accompany, enable and feed off globally oriented forms of cultural consumption. This article examines these claims in the light of unusually comprehensive data on the tastes of the white British population collected in a large national sample survey, in-depth interviews and focus groups. By identifying and analysing the geographical spread of the cultural referents of the tastes of the white British we make an empirical assessment of the claims for cosmopolitan identities. We argue that if white British identities are being reformed by processes of globalisation it is, paradoxically, in an increasingly Anglophone direction

Preliminary prediction of individual response to electroconvulsive therapy using whole-brain functional magnetic resonance imaging data.

Electroconvulsive therapy (ECT) works rapidly and has been widely used to treat depressive disorders (DEP). However, identifying biomarkers predictive of response to ECT remains a priority to individually tailor treatment and understand treatment mechanisms. This study used a connectome-based predictive modeling (CPM) approach in 122 patients with DEP to determine if pre-ECT whole-brain functional connectivity (FC) predicts depressive rating changes and remission status after ECT (47 of 122 total subjects or 38.5% of sample), and whether pre-ECT and longitudinal changes (pre/post-ECT) in regional brain network biomarkers are associated with treatment-related changes in depression ratings. Results show the networks with the best predictive performance of ECT response were negative (anti-correlated) FC networks, which predict the post-ECT depression severity (continuous measure) with a 76.23% accuracy for remission prediction. FC networks with the greatest predictive power were concentrated in the prefrontal and temporal cortices and subcortical nuclei, and include the inferior frontal (IFG), superior frontal (SFG), superior temporal (STG), inferior temporal gyri (ITG), basal ganglia (BG), and thalamus (Tha). Several of these brain regions were also identified as nodes in the FC networks that show significant change pre-/post-ECT, but these networks were not related to treatment response. This study design has limitations regarding the longitudinal design and the absence of a control group that limit the causal inference regarding mechanism of post-treatment status. Though predictive biomarkers remained below the threshold of those recommended for potential translation, the analysis methods and results demonstrate the promise and generalizability of biomarkers for advancing personalized treatment strategies

Joint and individual analysis of breast cancer histologic images and genomic covariates

A key challenge in modern data analysis is understanding connections between complex and differing modalities of data. For example, two of the main approaches to the study of breast cancer are histopathology (analyzing visual characteristics of tumors) and genetics. While histopathology is the gold standard for diagnostics and there have been many recent breakthroughs in genetics, there is little overlap between these two fields. We aim to bridge this gap by developing methods based on Angle-based Joint and Individual Variation Explained (AJIVE) to directly explore similarities and differences between these two modalities. Our approach exploits Convolutional Neural Networks (CNNs) as a powerful, automatic method for image feature extraction to address some of the challenges presented by statistical analysis of histopathology image data. CNNs raise issues of interpretability that we address by developing novel methods to explore visual modes of variation captured by statistical algorithms (e.g. PCA or AJIVE) applied to CNN features. Our results provide many interpretable connections and contrasts between histopathology and genetics

Phase Structures and Morphologies Determined by Competitions Among Self-Organization, Crystallization, and Vitrification in a Disordered Poly(Ethylene Oxide)-B-Polystyrene Diblock Copolymer

A poly(ethylene oxide)-b-polystyrene (PEO-b-PS) diblock copolymer having a number-average molecular weight ((M) over bar(n)) of 11000 g/mol in the PEO blocks and an (M) over bar(n) of 5200 g/mol in the PS blocks has been synthesized (with a volume fraction of the PEO blocks of 0.66 in the molten state). Differential scanning calorimetry results show that this copolymer possesses a single endotherm, which is attributed to the melting of the PEG-block crystals. Based on real-time resolved synchrotron small-angle x-ray scattering (SAXS) observations, the diblock copolymer is in a disordered state above the glass transition temperature of the PS-rich phase (T-g(PS)) which has been determined to be 44.0 degrees C during cooling using dilatometer mode in thermomechanical measurements. The order-disorder transition temperature (T-ODT) for this diblock copolymer is thus experimentally inaccessible. Depending upon different isothermal crystallization temperatures quenched from the disordered state (T(q)s), four cases can be investigated in order to understand the phase relationships among self-organization, crystallization of the PEO blocks, and vitrification of the PS-rich phase: the region where the T-q is above the T-g(PS), the regions where the T-q is near but slightly higher or lower than the T-g(PS) ; and the region where the T-q is below the T-g(PS) . Utilizing simultaneous SPXS and wide angle x-ray-diffraction experiments, it can be seen that lamellar crystals of the PEO blocks in the first case grow with little morphological constraint due to initial disordered phase morphology. As the T-q approaches but is still slightly higher than the T-g(PS) , as in the second case, the PEG-block crystals with a greater long period (L) than that of the disordered state start to grow. The initial disordered phase morphology is gradually destroyed, at least to a major extent. When the T-q is near but slightly lower than the T-g(PS), the crystallization takes place largely within the existing phase morphology. Only a gradual shift of the L towards smaller q values can be found with increasing time, which implies that the initial phase morphology is disturbed by the crystallization of the PEO blocks. In the last case, the PEO blocks crystallize under a total constraint provided by the disordered phase morphology due to rapid vitrification of the PS-rich phase. Substantial decrease of crystallinity can be observed in this case. This study also provides experimental evidence that the PS-rich phase size, which is down to 7-8 nm, can still retain bulky glassy properties. [S0163-1829(99)01138-8]

Subtentorial tumors and other lesions: An electroencephalographic study of 121 cases

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/32371/1/0000446.pd

HSD3B1 genotype identifies glucocorticoid responsiveness in severe asthma

Asthma resistance to glucocorticoid treatment is a major health problem with unclear etiology. Glucocorticoids inhibit adrenal androgen production. However, androgens have potential benefits in asthma. HSD3B1 encodes for 3β-hydroxysteroid dehydrogenase-1 (3β-HSD1), which catalyzes peripheral conversion from adrenal dehydroepiandrosterone (DHEA) to potent androgens and has a germline missense-encoding polymorphism. The adrenal restrictive HSD3B1(1245A) allele limits conversion, whereas the adrenal permissive HSD3B1(1245C) allele increases DHEA metabolism to potent androgens. In the Severe Asthma Research Program (SARP) III cohort, we determined the association between DHEA-sulfate and percentage predicted forced expiratory volume in 1 s (FEV1PP). HSD3B1(1245) genotypes were assessed, and association between adrenal restrictive and adrenal permissive alleles and FEV1PP in patients with (GC) and without (noGC) daily oral glucocorticoid treatment was determined (n = 318). Validation was performed in a second cohort (SARP I&II; n = 184). DHEA-sulfate is associated with FEV1PP and is suppressed with GC treatment. GC patients homozygous for the adrenal restrictive genotype have lower FEV1PP compared with noGC patients (54.3% vs. 75.1%; P < 0.001). In patients with the homozygous adrenal permissive genotype, there was no FEV1PP difference in GC vs. noGC patients (73.4% vs. 78.9%; P = 0.39). Results were independently confirmed: FEV1PP for homozygous adrenal restrictive genotype in GC vs. noGC is 49.8 vs. 63.4 (P < 0.001), and for homozygous adrenal permissive genotype, it is 66.7 vs. 67.7 (P = 0.92). The adrenal restrictive HSD3B1(1245) genotype is associated with GC resistance. This effect appears to be driven by GC suppression of 3β-HSD1 substrate. Our results suggest opportunities for prediction of GC resistance and pharmacologic intervention