Cloud media video encoding:review and challenges

In recent years, Internet traffic patterns have been changing. Most of the traffic demand by end users is multimedia, in particular, video streaming accounts for over 53%. This demand has led to improved network infrastructures and computing architectures to meet the challenges of delivering these multimedia services while maintaining an adequate quality of experience. Focusing on the preparation and adequacy of multimedia content for broadcasting, Cloud and Edge Computing infrastructures have been and will be crucial to offer high and ultra-high definition multimedia content in live, real-time, or video-on-demand scenarios. For these reasons, this review paper presents a detailed study of research papers related to encoding and transcoding techniques in cloud computing environments. It begins by discussing the evolution of streaming and the importance of the encoding process, with a focus on the latest streaming methods and codecs. Then, it examines the role of cloud systems in multimedia environments and provides details on the cloud infrastructure for media scenarios. After doing a systematic literature review, we have been able to find 49 valid papers that meet the requirements specified in the research questions. Each paper has been analyzed and classified according to several criteria, besides to inspect their relevance. To conclude this review, we have identified and elaborated on several challenges and open research issues associated with the development of video codecs optimized for diverse factors within both cloud and edge architectures. Additionally, we have discussed emerging challenges in designing new cloud/edge architectures aimed at more efficient delivery of media traffic. This involves investigating ways to improve the overall performance, reliability, and resource utilization of architectures that support the transmission of multimedia content over both cloud and edge computing environments ensuring a good quality of experience for the final user

Electrical conductivity measurements of films

Póster presentado en la 12th European Conference on Thermoelectrics (ECT2014), celebrada en Madrid del 24 al 26 de septiembre de 2014.The characterization of the electrical conductivity of thin films is mandatory in all materials but particularly in thermoelectricity, its measurement is crucial in order to be able to determine the power factor and the figure of merit. A technique that could be used to carry out electrical measurements on thin films is the four probe technique. However, the spreading of the current due to the electrical field or the influence of the electrical contact resistances complicate the determination and analysis of the electrical conductivity of the film. In order to overcome these problems, we carried out a mesa attack on Bi2Te3 films grown by electrodeposition technique, which are hold on a Si substrate with a gold layer of 150nm. The goal is fabricating pillars whose later film resistivity analysis could approach to the 1D electrical model, which cannot be taken into account when dealing with a big film area. For that purpose, a lithography process was done on films with different thicknesses, which consist of a pattern of disks with different diameters ranging between 120µm and 60µm diameter. After the lithography, we evaporated 100nm of gold on top of the disks that would act as the top electrode. Then, we removed the photoresist and we performed a mesa attack with diluted nitric acid (1:2.5). As a result, we obtained pillars as the ones showed in Figure 1. On these pillars, I-V curves with the four probe technique were taken and the resistances of the pillars were determined. Representing the resistance of the pillars versus the inverse of the area of the pillar, the total resistivity of the films, which includes the contact resistances between the electrodes and the pillar, is obtained. Finally, to determine the electrical contact resistance and obtain the pure electrical resistivity of the film, these measurements were performed on different pillar thicknesses. The experimental results have been double-checked with COMSOL simulations of the four probe experiments carried out here, observing a good agreement between them.Peer Reviewe

Expression of a Mutant p53 Results in an Age-Related Demographic Shift in Spontaneous Lung Tumor Formation in Transgenic Mice

BACKGROUND:Mutations in the P53 gene are among the most common genetic abnormalities in human lung cancer. Codon 273 in the sequence-specific DNA binding domain is one of the most frequently mutated sites. METHODOLOGY:To investigate the role of mutant p53 in lung tumorigenesis, a lung specific p53(273H) transgenic mouse model was developed. Rates of lung cancer formation in the transgenic animals and their littermates were evaluated by necropsy studies performed in progressive age cohorts ranging from 4 to 24 months. In order to establish the influence of other common genetic abnormalities in lung tumor formation in the animals, K-Ras gene mutation and p16INK4a (p16) promoter methylation were evaluated in a total of 281 transgenic mice and 189 non-transgenic littermates. PRINCIPAL FINDINGS:At the age extremes of 4-12 and 22-24 months no differences were observed, with very low prevalence of tumors in animals younger than 12 months, and a relatively high prevalence at age 22 months or older. However, the transgenic mice had a significant higher lung tumor rate than their non-transgenic counterparts during the age of 13-21 months, suggesting an age-related shift in lung tumor formation induced by the lung-specific expression of the human mutant p53. Histopathology suggested a more aggressive nature for the transgenic tumors. Older mice (>13 months) had a significantly higher rate of p16 promoter methylation (17% v 82%). In addition, an age related effect was observed for K-Ras codons 12 or 13 mutations, but not for codon 61 mutations. CONCLUSIONS/SIGNIFICANCE:These results would suggest that the mutant p53(273H) contributes to an acceleration in the development of spontaneous lung tumors in these mice. Combination with other genetic and epigenetic alterations occurring after the age of 13 months is intimately linked to its oncogenic potential

Shoc2/Sur8 protein regulates neurite outgrowth

This is an openaccess article distributed under the terms of the Creative Commons Attribution License.-- et al.The Shoc2 protein has been implicated in the positive regulation of the Ras-ERK pathway by increasing the functional binding interaction between Ras and Raf, leading to increased ERK activity. Here we found that Shoc2 overexpression induced sustained ERK phosphorylation, notably in the case of EGF stimulation, and Shoc2 knockdown inhibited ERK activation. We demonstrate that ectopic overexpression of human Shoc2 in PC12 cells significantly promotes neurite extension in the presence of EGF, a stimulus that induces proliferation rather than differentiation in these cells. Finally, Shoc2 depletion reduces both NGF-induced neurite outgrowth and ERK activation in PC12 cells. Our data indicate that Shoc2 is essential to modulate the Ras-ERK signaling outcome in cell differentiation processes involved in neurite outgrowth.GL, TG and LMD were recipients of fellowships from the Ministerio de Educación y Ciencia (MEC) (to GL, TG), and Fondo de Investigaciones Sanitarias (FIS) (to LMD). LSR held a postdoctoral research contract from CIBERNED. This work was supported by FIS grant (PI10/00815) to JLO; CIBERNED to MC; SAF2008-01951, Comunidad Autónoma de Madrid (CAM) SSAL-0202-2006-01 and Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED) to TI; FIS grant PI12/00775 and ISCIII-RETIC (Red Temática de Investigación Cooperativa en Cáncer) RD12/0036/0027 from the Instituto de Salud Carlos III to PSG; and FIS grants (PI09/0562 and PI13/00703), ISCIIIRETIC (RD06/0020/0003 and RD12/0036/0021), and the Spanish Association Against Cancer (AECC) to JMR.Peer Reviewe

Separación in situ de heces y orina mediante cinta de deyecciones bajo slat en alojamiento de cebo de ganado porcino

Se analizan los resultados de producción de heces y orina en dos ciclos de cebo completo con 168 animales utilizando un sistema de cinta de separacion de heces y orina bajo slat, patentado por parte de los miembros del equipo. Se estudia la influencia del tipo de cinta, de la inclinación de la misma y frecuencia de extracción. Tambien se controló la temepratura del aire tanto a la entrada como a la salida del alojamiento. Con el sistema se logra una eficaz separación de heces y orina, con una disminución notable en la producción de ambos residuos. La ventilaci¿on forzada permite reducir la cantidad de residuos generados

A numerical model analysis of the tidal flows in the Bay of Algeciras, Strait of Gibraltar

A numerical model has been applied to study the tidal flows of the Bay of Algeciras in the eastern part of the Strait of Gibraltar, focusing on the M-2 semidiurnal constituent. The numerical model was satisfactorily validated against a comprehensive set of observations collected in the bay in the year 2011 and the model outputs were used for a detailed analysis of the local tidal circulation. The M-2 net (vertically integrated) transport across the mouth of the bay has an amplitude of 2.7 x 10(-3) Sv, while that of the sea surface signal is of similar to 30 cm and is in quadrature with this flow. However, the vertically integrated flow is the result of a pronounced baroclinic structure consisting of an upper (S 37.5) layers, whose associated transports are one order of magnitude higher. This reveals a noticeable internal tide that is characterized by an inward (to the head of the bay) propagation and a likely quarter-wave resonance. During the rising tide, Atlantic water from the strait comes in and produces the thickening of the upper (Atlantic) layer in the bay, while Mediterranean water of the lower layer is pushed out to join the Mediterranean water stream that is flowing to the west along the Strait of Gibraltar. During the falling tide, Atlantic water flows out of the bay and incorporates to the eastward flow in the strait. In this tidal phase, Mediterranean water flows into the bay. Therefore, Atlantic and Mediterranean waters accumulate in the bay during the rising and falling tide, respectively. This pattern is opposite to that observed in the strait, where the Mediterranean layer thickens during the rising tide and becomes thinner during the falling tide. This suggests that the internal tide in the bay is basically determined by the baroclinic forcing at its mouth imposed by the baroclinic tide of the Strait of Gibralta

Human blood-brain barrier receptors for Alzheimer's amyloid-beta 1- 40. Asymmetrical binding, endocytosis, and transcytosis at the apical side of brain microvascular endothelial cell monolayer.

This is the published version. Copyright 1998 by American Society for Clinical Investigation.A soluble monomeric form of Alzheimer's amyloid-beta (1-40) peptide (sAbeta1-40) is present in the circulation and could contribute to neurotoxicity if it crosses the brain capillary endothelium, which comprises the blood-brain barrier (BBB) in vivo. This study characterizes endothelial binding and transcytosis of a synthetic peptide homologous to human sAbeta1-40 using an in vitro model of human BBB. 125I-sAbeta1-40 binding to the brain microvascular endothelial cell monolayer was time dependent, polarized to the apical side, and saturable with high- and low-affinity dissociation constants of 7.8+/-1.2 and 52.8+/-6.2 nM, respectively. Binding of 125I-sAbeta1-40 was inhibited by anti-RAGE (receptor for advanced glycation end products) antibody (63%) and by acetylated low density lipoproteins (33%). Consistent with these data, transfected cultured cells overexpressing RAGE or macrophage scavenger receptor (SR), type A, displayed binding and internalization of 125I-sAbeta1-40. The internalized peptide remains intact > 94%. Transcytosis of 125I-sAbeta1-40 was time and temperature dependent, asymmetrical from the apical to basolateral side, saturable with a Michaelis constant of 45+/-9 nM, and partially sensitive to RAGE blockade (36%) but not to SR blockade. We conclude that RAGE and SR mediate binding of sAbeta1-40 at the apical side of human BBB, and that RAGE is also involved in sAbeta1-40 transcytosis