152 research outputs found
Japanese Pop Culture, Identification, and Socialization: The Case of an Italian Web - Community
Japanese pop culture has influenced Italy over the last thirty years. In the â70s anime started to fill the airtime of emerging private TV channels, marking the childhood of those Italians who grew up in those years and until the early â90s, when manga finally appeared in the Italian market. Globalization and the Internet have made other aspects of Japanese pop culture available to Italians and the rest of the world alike. It has resulted in a very active Italian fandom spanning different generations, and in a strong fascination with Japan.
This paper aims to provide insights into the way Italian fans perceive Japanese pop culture and Japan; on the kind of bonds with Japan they develop, and how they socialize. It does so by considering the biggest Italian web-community, AnimeClick.it, as a microcosm of the Italian fandomâs interactions and emotions. Privileging a qualitative method, it focuses on the people who give life to the website. Their images of Japanese pop culture reveal the recognition of a specific cultural odour perceived as pleasant, which translates into an interest in Japan. Those fans associate Japan with images of fantasy and charming mystery that nevertheless co-exist with perceptions of extreme difference, echoing the notion of Japanese uniqueness, so that Orientalist processes are re-enacted. There are intergenerational differences in the way fans have developed an emotional bond, and look at Japanese pop culture. However, these are mediated and transcended through their socialization and collaboration in the web-community, opening up new perspectives for the future evolution of Japanese pop cultureâs influence in Italy
Deep Neural Network Equalization for Optical Short Reach Communication
Nonlinear distortion has always been a challenge for optical communication due to the
nonlinear transfer characteristics of the ďŹber itself. The next frontier for optical communication is a
second type of nonlinearities, which results from optical and electrical components. They become the
dominant nonlinearity for shorter reaches. The highest data rates cannot be achieved without effective
compensation. A classical countermeasure is receiver-side equalization of nonlinear impairments
and memory effects using Volterra series. However, such Volterra equalizers are architecturally
complex and their parametrization can be numerical unstable. This contribution proposes an
alternative nonlinear equalizer architecture based on machine learning. Its performance is evaluated
experimentally on coherent 88 Gbaud dual polarization 16QAM 600 Gb/s back-to-back measurements.
The proposed equalizers outperform Volterra and memory polynomial Volterra equalizers up to 6th
orders at a target bit-error rate (BER) of 10
â2
by 0.5 dB and 0.8 dB in optical signal-to-noise ratio
(OSNR), respectively
Amaranthus grain as a new ingredient in diets for dairy cows: productive, qualitative, and in vitro fermentation traits
Background: In recent decades, grain amaranths have attracted attention due to their valuable combination of nutritional traits, with higher protein and oil content than conventional cereals. Before they can be proposed as an unconventional ingredient in animal feed, many aspects still need to be investigated from field production to nutritive value. The present research aimed to study the agronomic traits, proximate composition, and digestibility/degradability, fatty acid profile, antioxidant activity, and total phenolic content of two grain amaranth species, Amaranthus cruentus and Amaranthus hypochondriacus (for a total of six accessions), grown in a Mediterranean environment. Results: Both species showed seed yields comparable to or higher than the traditional cereal crops in the same environment. On the whole, A. cruentus resulted in a higher seed production than A. hypochondriacus. Mexico and Montana accessions, both belonging to A. cruentus, showed the highest yield (3.73âtâha-1 , on average). Few differences emerged in nutritive value between species and accessions: the Illinois accession of A. cruentus showed the best performance in terms of in vitro degradability and gas production, but not for volatile fatty acid production; the fermentation kinetics was slowest in the Illinois accession and fastest in the Montana accession of A. cruentus and the India accession of A. hypochondriacus. Conclusion: From a health perspective, the Nebraska accession of A. hypochondriacus represents the best accession, with the lowest saturated fatty acid content and the highest polyunsaturated fatty acid content. Š 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry
An overview of molecular mechanisms in fabry disease
Fabry disease (FD) (OMIM #301500) is a rare genetic lysosomal storage disorder (LSD). LSDs are characterized by inappropriate lipid accumulation in lysosomes due to specific enzyme deficiencies. In FD, the defective enzyme is alpha-galactosidase A (alpha-Gal A), which is due to a mutation in the GLA gene on the X chromosome. The enzyme deficiency leads to a continuous deposition of neutral glycosphingolipids (globotriaosylceramide) in the lysosomes of numerous tissues and organs, including endothelial cells, smooth muscle cells, corneal epithelial cells, renal glomeruli and tubules, cardiac muscle and ganglion cells of the nervous system. This condition leads to progressive organ failure and premature death. The increasing understanding of FD, and LSD in general, has led in recent years to the introduction of enzyme replacement therapy (ERT), which aims to slow, if not halt, the progression of the metabolic disorder. In this review, we provide an overview of the main features of FD, focusing on its molecular mechanism and the role of biomarkers
Exploratory pilot study of circulating biomarkers in metastatic renal cell carcinoma
With the introduction of immune checkpoint inhibitors (ICIs) and next-generation vascular endothelial growth factor receptorâtyrosine kinase inhibitors (VEGFRâTKIs), the survival of patients with advanced renal cell carcinoma (RCC) has improved remarkably. However, not all patients have benefited from treatments, and to date, there are still no validated biomarkers that can be included in the therapeutic algorithm. Thus, the identification of predictive biomarkers is necessary to increase the number of responsive patients and to understand the underlying immunity. The clinical outcome of RCC patients is, in fact, associated with immune response. In this exploratory pilot study, we assessed the immune effect of TKI therapy in order to evaluate the immune status of metastatic renal cell carcinoma (mRCC) patients so that we could define a combination of immunological biomarkers relevant to improving patient outcomes. We profiled the circulating levels in 20 mRCC patients of exhausted/activated/regulatory T cell subsets through flow cytometry and of 14 immune checkpoint-related proteins and 20 inflammation cytokines/chemokines using multiplex Luminex assay, both at baseline and during TKI therapy. We identified the CD3+CD8+CD137+ and CD3+CD137+PD1+ T cell populations, as well as seven soluble immune molecules (i.e., IFNÎł, sPDL2, sHVEM, sPD1, sGITR, sPDL1, and sCTLA4) associated with the clinical responses of mRCC patients, either modulated by TKI therapy or not. These results suggest an immunological profile of mRCC patients, which will help to improve clinical decision-making for RCC patients in terms of the best combination of strategies, as well as the optimal timing and therapeutic sequence
New prognostic model in patients with advanced urothelial carcinoma treated with second-line immune checkpoint inhibitors
Background: Bellmunt Risk Score, based on Eastern Cooperative Oncology Group (ECOG) performance status (PS), hemoglobin levels and presence of liver metastases, is the most established prognostic algorithm for patients with advanced urothelial cancer (aUC) progressing after platinum-based chemotherapy. Nevertheless, existing algorithms may not be sufficient following the introduction of immunotherapy. Our aim was to develop an improved prognostic model in patients receiving second-line atezolizumab for aUC. Methods: Patients with aUC progressing after cisplatin/carboplatin-based chemotherapy and enrolled in the prospective, single-arm, phase IIIb SAUL study were included in this analysis. Patients were treated with 3-weekly atezolizumab 1200 mg intravenously. The development and internal validation of a prognostic model for overall survival (OS) was performed using Cox regression analyses, bootstrapping methods and calibration. Results: In 936 patients, ECOG PS, alkaline phosphatase, hemoglobin, neutrophil-to-lymphocyte ratio, liver metastases, bone metastases and time from last chemotherapy were identified as independent prognostic factors. In a 4-tier model, median OS for patients with 0â1, 2, 3â4 and 5â7 risk factors was 18.6, 10.4, 4.8 and 2.1 months, respectively. Compared with Bellmunt Risk Score, this model provided enhanced prognostic separation, with a c-index of 0.725 vs 0.685 and increment in c-statistic of 0.04 (p<0.001). Inclusion of PD-L1 expression did not improve the model. Conclusions: We developed and internally validated a prognostic model for patients with aUC receiving postplatinum immunotherapy. This model represents an improvement over the Bellmunt algorithm and could aid selection of patients with aUC for second-line immunotherapy. Trial registration number: NCT02928406
A Regulatory Mechanism Involving TBP-1/Tat-Binding Protein 1 and Akt/PKB in the Control of Cell Proliferation
TBP-1 /Tat-Binding Protein 1 (also named Rpt-5, S6a or PSMC3) is a multifunctional protein, originally identified as a regulator of HIV-1-Tat mediated transcription. It is an AAA-ATPase component of the 19S regulative subunit of the proteasome and, as other members of this protein family, fulfils different cellular functions including proteolysis and transcriptional regulation. We and others reported that over expression of TBP-1 diminishes cell proliferation in different cellular contexts with mechanisms yet to be defined. Accordingly, we demonstrated that TBP-1 binds to and stabilizes the p14ARF oncosuppressor increasing its anti-oncogenic functions. However, TBP-1 restrains cell proliferation also in the absence of ARF, raising the question of what are the molecular pathways involved. Herein we demonstrate that stable knock-down of TBP-1 in human immortalized fibroblasts increases cell proliferation, migration and resistance to apoptosis induced by serum deprivation. We observe that TBP-1 silencing causes activation of the Akt/PKB kinase and that in turn TBP-1, itself, is a downstream target of Akt/PKB. Moreover, MDM2, a known Akt target, plays a major role in this regulation. Altogether, our data suggest the existence of a negative feedback loop involving Akt/PKB that might act as a sensor to modulate TBP-1 levels in proliferating cells
Twelve Variants Polygenic Score for Low-Density Lipoprotein Cholesterol Distribution in a Large Cohort of Patients With Clinically Diagnosed Familial Hypercholesterolemia With or Without Causative Mutations
: Background A significant proportion of individuals clinically diagnosed with familial hypercholesterolemia (FH), but without any disease-causing mutation, are likely to have polygenic hypercholesterolemia. We evaluated the distribution of a polygenic risk score, consisting of 12 low-density lipoprotein cholesterol (LDL-C)-raising variants (polygenic LDL-C risk score), in subjects with a clinical diagnosis of FH. Methods and Results Within the Lipid Transport Disorders Italian Genetic Network (LIPIGEN) study, 875 patients who were FH-mutation positive (women, 54.75%; mean age, 42.47Âą15.00Â years) and 644 patients who were FH-mutation negative (women, 54.21%; mean age, 49.73Âą13.54Â years) were evaluated. Patients who were FH-mutation negative had lower mean levels of pretreatment LDL-C than patients who were FH-mutation positive (217.14Âą55.49 versus 270.52Âą68.59Â mg/dL, P<0.0001). The mean value (ÂąSD) of the polygenic LDL-C risk score was 1.00 (Âą0.18) in patients who were FH-mutation negative and 0.94 (Âą0.20) in patients who were FH-mutation positive (P<0.0001). In the receiver operating characteristic analysis, the area under the curve for recognizing subjects characterized by polygenic hypercholesterolemia was 0.59 (95% CI, 0.56-0.62), with sensitivity and specificity being 78% and 36%, respectively, at 0.905 as a cutoff value. Higher mean polygenic LDL-C risk score levels were observed among patients who were FH-mutation negative having pretreatment LDL-C levels in the range of 150 to 350Â mg/dL (150-249Â mg/dL: 1.01 versus 0.91, P<0.0001; 250-349Â mg/dL: 1.02 versus 0.95, P=0.0001). A positive correlation between polygenic LDL-C risk score and pretreatment LDL-C levels was observed among patients with FH independently of the presence of causative mutations. Conclusions This analysis confirms the role of polymorphisms in modulating LDL-C levels, even in patients with genetically confirmed FH. More data are needed to support the use of the polygenic score in routine clinical practice
Valutazione di Health Technology Assessment del sistema di sanificazione biologico a base di probiotici del genere Bacillus (PCHS)
Le infezioni correlate allâassistenza: prioritĂ per la salute pubblica
Epidemiologia delle infezioni correlate allâassistenza in Italia e loro impatto per la salute pubblica
Sistemi di sanificazione attualmente disponibili in Italia
Il Probiotic Cleaning Hygiene System (PCHS): caratteristiche della tecnologia, aspetti di efficacia e sicurezza
Un sistema di sanificazione a base di probiotici per la riduzione delle infezioni correlate allâassistenza e la resistenza antimicrobica: analisi dellâimpatto sul budget
Impatto ambientale per la salute pubblica degli attuali sistemi di sanificazione di ambienti/superfici in setting assistenziale e comunitario e potenziali benefici dei nuovi sistemi innovativi
Analisi delle principali raccomandazioni nazionali su sanificazione e disinfezione degli ambienti sanitari
Valutazione etica dellâutilizzo del Probiotic Cleaning Hygiene System (PCHS) in Italia
Elementi chiave per il processo decisional
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