Genome‑wide distribution of histone trimethylation reveals a global impact of bisphenol A on telomeric binding proteins and histone acetyltransferase factors: a pilot study with human and in vitro data

Objective: To assess the genetic and epigenetic effects promoted by Bisphenol A (BPA) exposure in adolescent males from the Spanish INMA-Granada birth cohort, and in human cells. Methods: DNA methylation was analysed using MEDIP. Repeat number variation in genomic DNA was evaluated, along with the analysis of H3K4me3 by using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq). Analyses were performed with material extracted from whole blood of the adolescents, complemented by in vitro assessments of human (HeLa) cells exposed to 10 nM BPA, specifically, immunofluorescence evaluation of protein levels, gene expression analysis and ChIP‒qPCR analysis. Results: Adolescents in the high urinary BPA levels group presented a higher level of Satellite A (SATA) repetitive region copy numbers compared to those in the low BPA group and a tendency towards increase in telomere length. We also observed decreased DNA methylation at the promoters of the imprinted genes H19, KCNQ1, and IGF2; at LINE1 retroelements; and at the ARID2, EGFR and ESRRA and TERT genes. Genome-wide sequencing revealed increased H3K4me3 occupancy at the promoters of genes encoding histone acetyltransferases, telomeric DNA binding factors and DNA repair genes. Results were supported in HeLa cells exposed to 10 nM BPA in vitro. In accordance with the data obtained in blood samples, we observed higher H3K4me3 occupancy and lower DNA methylation at some specific targets in HeLa cells. In exposed cells, changes in the expression of genes encoding DNA repair factors (ATM, ARID2, TRP53) were observed, and increased expression of several genes encoding telomeric DNA binding factors (SMG7, TERT, TEN1, UPF1, ZBTB48) were also found. Furthermore, an increase in ESR1/ERa was observed in the nuclei of HeLa cells along with increased binding of ESR1 to KAT5, KMT2E and TERF2IP promoters and decreased ESR1 binding at the RARA promoter. The DNA damage marker p53/TP53 was also increased. Conclusion: In this pilot study, genome-wide analysis of histone trimethylation in adolescent males exposed to BPA revealed a global impact on the expression of genes encoding telomeric binding proteins and histone acetyltransferase factors with similar results in HeLa cells. Nevertheless, larger studies should confirm our findings.European Commission E17013NKInstitut National de la Sante et de la Recherche Medicale (Inserm) European CommissionNational Natural Science Foundation of China (NSFC) 3180106

Composición taxonómica y variación estacional del zooplancton del embalse Piedras Moras (Córdoba, Argentina)

Reservoirs are water bodies relatively similar to natural lakes, differing in aspects such as the longitudinal gradient of certain environmental and biotic factors. In the mountainous region of Córdoba there are more than 15000 ha of reservoirs. Although aspects of these reservoirs have been studied, the zooplankton has generally not received much attention. The aim of this study was to determine the composition and seasonal variation of the zooplankton of Piedras Moras reservoir, a multipurpose reservoir built on the Ctalamochita River, in which this community had not been previously studied. Seasonal samples were taken (November 2009 - September 2010) at three sites. The richness was 20 taxa and the highest diversity was recorded in the center of the reservoir. The typical association of the center-northwest of the country reservoirs was found. The crustaceans Notodiaptomus incompositus, Metacyclops mendocinus, Ceriodaphnia dubia y Bosmina longirostris were recorded in the majority of the samples and, although they were the more frequent rotifers, Brachionus plicatilis, B. dimidiatus, B. caudatus and Polyarthra sp. were found less frequently. In the center, cladocerans were dominant in the summer and autumn, and copepods were dominant in the spring and winter. Though longitudinal zonation was found, this was based only on information from the summer period.Los embalses son cuerpos de agua relativamente similares a los lagos naturales, que se diferencian en aspectos como el gradiente longitudinal de ciertos factores ambientales y bióticos. En la región montañosa de Córdoba hay más de 15000 ha de embalses. Aunque se han estudiado aspectos de estos ambientes, el zooplancton generalmente no ha recibido mucha atención. El objetivo de este estudio fue determinar la composición y la variación estacional del zooplancton del embalse de Piedras Moras, un ambiente multipropósito construido en el río Calamuchita, en el que esta comunidad no se había estudiado previamente. Se tomaron muestras estacionales (noviembre de 2009 - septiembre de 2010) en tres sitios. La riqueza fue de 20 taxones y la mayor diversidad se registró en el centro del embalse. Se encontró la asociación típica de los embalses centro-noroeste del país. Los crustáceos Notodiaptomus incompositus, Metacyclops mendocinus, Ceriodaphnia dubia y Bosmina longirostris se registraron en la mayoría de las muestras y, aunque fueron los rotíferos más frecuentes, Brachionus plicatilis, B. dimidiatus, B. caudatus y Polyarthra sp. se encontraron con menos frecuencia. En el centro, los cladóceros eran dominantes en verano y otoño, y los copépodos eran dominantes en la primavera y el invierno. Aunque se encontró zonación longitudinal, esto se basó solo en la información del período de verano

Composición taxonómica y variación estacional del zooplancton del embalse Piedras Moras (Córdoba, Argentina)

Reservoirs are water bodies relatively similar to natural lakes, differing in aspects such as the longitudinal gradient of certain environmental and biotic factors. In the mountainous region of Córdoba there are more than 15000 ha of reservoirs. Although aspects of these reservoirs have been studied, the zooplankton has generally not received much attention. The aim of this study was to determine the composition and seasonal variation of the zooplankton of Piedras Moras reservoir, a multipurpose reservoir built on the Ctalamochita River, in which this community had not been previously studied. Seasonal samples were taken (November 2009 - September 2010) at three sites. The richness was 20 taxa and the highest diversity was recorded in the center of the reservoir. The typical association of the center-northwest of the country reservoirs was found. The crustaceans Notodiaptomus incompositus, Metacyclops mendocinus, Ceriodaphnia dubia y Bosmina longirostris were recorded in the majority of the samples and, although they were the more frequent rotifers, Brachionus plicatilis, B. dimidiatus, B. caudatus and Polyarthra sp. were found less frequently. In the center, cladocerans were dominant in the summer and autumn, and copepods were dominant in the spring and winter. Though longitudinal zonation was found, this was based only on information from the summer period.Facultad de Ciencias Naturales y Muse

Composición taxonómica y variación estacional del zooplancton del embalse Piedras Moras (Córdoba, Argentina)

Reservoirs are water bodies relatively similar to natural lakes, differing in aspects such as the longitudinal gradient of certain environmental and biotic factors. In the mountainous region of Córdoba there are more than 15000 ha of reservoirs. Although aspects of these reservoirs have been studied, the zooplankton has generally not received much attention. The aim of this study was to determine the composition and seasonal variation of the zooplankton of Piedras Moras reservoir, a multipurpose reservoir built on the Ctalamochita River, in which this community had not been previously studied. Seasonal samples were taken (November 2009 - September 2010) at three sites. The richness was 20 taxa and the highest diversity was recorded in the center of the reservoir. The typical association of the center-northwest of the country reservoirs was found. The crustaceans Notodiaptomus incompositus, Metacyclops mendocinus, Ceriodaphnia dubia y Bosmina longirostris were recorded in the majority of the samples and, although they were the more frequent rotifers, Brachionus plicatilis, B. dimidiatus, B. caudatus and Polyarthra sp. were found less frequently. In the center, cladocerans were dominant in the summer and autumn, and copepods were dominant in the spring and winter. Though longitudinal zonation was found, this was based only on information from the summer period.Facultad de Ciencias Naturales y Muse

Aplicación de estrategias didácticas inclusivas por parte del docente en el proceso de formación académica y su incidencia en el desarrollo de competencias personales y sociales en los estudiantes universitarios con discapacidad durante el año 2020

Las estrategias didácticas inclusivas por parte del docente se han vuelto una de las formas más eficaces para optimizar las practicas pedagógicas, permitiendo que el docente pueda acercarse a cada uno de sus estudiantes atendiendo sus necesidades, de esta forma lograr el desarrollo de las competencias sociales y personales. En consecución de este planteamiento se desarrolló una investigación que permitió conocer cada una de estas estrategias, bajo un método de análisis documental, es decir un proceso que parte de la selección de diferentes fuentes de información relacionadas al tema de investigación del cual se realizó dicho análisis, partiendo de sus fundamentos científicos y analíticos. Por lo tanto se retoma la importancia de llevar a cabo una educación inclusiva, que garantice logros educativos e institucionales, además del desarrollo de las competencias básicas que conlleva la educación, permitiendo mejorar la enseñanza de las instituciones educativas, al mismo tiempo se identifican diferentes estrategias didácticas inclusivas virtuales las cuales permiten elevar su uso y efectividad al momento de adoptar tecnología y virtualización, siendo así una excelente opción ante la situación de la pandemia actual de Covid-19, teniéndola oportunidad de llevar a cabo una educación inclusiva, innovadora y de calidad, mediante la utilización delos recursos tecnológicos que permitan que la población estudiantil pueda tener una formación integral y exitosa, no importando el tipo de discapacidad que este tenga. Logrando así establecer un enfoque de atención a la diversidad demostrando el papel importante que tiene el docente para la atención de personas con discapacida

7 años de DarocaFarmacias: programa de coordinación entre el Centro de Salud Daroca y los Farmacéuticos Comunitarios de su Zona Básica de Salud.

RESUMEN    OBJETIVO Evaluar el funcionamiento y el grado de satisfacción de los profesionales implicados en un programa de colaboración entre el Centro de Salud Daroca y las Farmacias Comunitarias de su zona de influencia (DarocaFarmacias) en Madrid, España.    MÉTODOS Participan 36 oficinas de farmacia y 81 profesionales sanitarios del centro de salud Daroca en un área urbana con 53.600 pacientes. Existe un correo electrónico y un teléfono directo en el centro de salud para facilitar la comunicación con las farmacias. Se realizan conjuntamente trabajos de investigación, sesiones de formación y actividades de educación para la salud.  Se preguntó sobre los diferentes aspectos del proyecto (comunicación, relación interprofesional, sesiones de formación y satisfacción) mediante una encuesta anónima a los integrantes de DarocaFarmacias.     RESULTADOS Contestaron 67 profesionales (32 farmacéuticos, 21 médicos y 13 enfermeros). El 95,5% cree que ha mejorado la relación entre profesionales y el 82% que ha favorecido la comunicación. Para el 43,7% de los farmacéuticos la mejor vía de comunicación es el teléfono (92% de llamadas resolutivas), para el 31,2% el correo electrónico y el 53,1% ha acudido alguna vez al centro para resolver el problema. Acude a las sesiones de formación el 82% y resultan muy útiles para el 92,5%. El 91% de los profesionales está bastante o muy satisfecho con DarocaFarmacias.   CONCLUSIÓN DarocaFarmacias es un ejemplo de colaboración entre centros de salud y oficinas de farmacia en aras de una mejor atención a los pacientes.     ABSTRACT   OBJECTIVE To assess the performance and degree of satisfaction of the healthcare professionals involved in a cooperation programme between Daroca Health Centre and Community Pharmaceuticals in a Basic Health Area (DarocaFarmacias) in Madrid, Spain.   METHODS Thirty-six community pharmacies and 81 health care professionals of the Daroca Health Centre took part in this study, affecting an urban area of 53600 patients. Communications between Health Centre and Community Pharmacies were done by email and direct phone. Researching, clinical sessions and educational activities were collaboratively carried out. Different aspects of the project were analysed, such as communication, interprofessional relationship, training sessions and global satisfaction. With this aim, a blinded survey was submitted to the DarocaFarmacias members.   RESULTS Sixty-seven health professionals took part (32 community pharmacists, 21 physicians and 12 nurses) in the study. Among them, 95.5% are of the opinion that professional relationship improved and 82% agreed for a better-quality communication between health professionals. Most of the community pharmacists (43.7%) considered telephone as the preferred channel of communication (92% problem solving), followed by e-mail (31.2%), although half of them (53.1%) visited the Health Centre to solve a relevant question. Eighty-two out of 100 attended to the clinical sessions and educational activities, being considered as beneficial by 92.5% of them. Over nine out of ten (91%) of the health professionals were quite or very satisfied with DarocaFarmacias project.   CONCLUSION DarocaFarmacias is a positive experience of collaboration between Health Centre and community pharmacies in pursuit of a better attendance of the patients

7 años de DarocaFarmacias: programa de coordinación entre el Centro de Salud Daroca y los Farmacéuticos Comunitarios de su Zona Básica de Salud.

RESUMEN    OBJETIVO Evaluar el funcionamiento y el grado de satisfacción de los profesionales implicados en un programa de colaboración entre el Centro de Salud Daroca y las Farmacias Comunitarias de su zona de influencia (DarocaFarmacias) en Madrid, España.    MÉTODOS Participan 36 oficinas de farmacia y 81 profesionales sanitarios del centro de salud Daroca en un área urbana con 53.600 pacientes. Existe un correo electrónico y un teléfono directo en el centro de salud para facilitar la comunicación con las farmacias. Se realizan conjuntamente trabajos de investigación, sesiones de formación y actividades de educación para la salud.  Se preguntó sobre los diferentes aspectos del proyecto (comunicación, relación interprofesional, sesiones de formación y satisfacción) mediante una encuesta anónima a los integrantes de DarocaFarmacias.     RESULTADOS Contestaron 67 profesionales (32 farmacéuticos, 21 médicos y 13 enfermeros). El 95,5% cree que ha mejorado la relación entre profesionales y el 82% que ha favorecido la comunicación. Para el 43,7% de los farmacéuticos la mejor vía de comunicación es el teléfono (92% de llamadas resolutivas), para el 31,2% el correo electrónico y el 53,1% ha acudido alguna vez al centro para resolver el problema. Acude a las sesiones de formación el 82% y resultan muy útiles para el 92,5%. El 91% de los profesionales está bastante o muy satisfecho con DarocaFarmacias.   CONCLUSIÓN DarocaFarmacias es un ejemplo de colaboración entre centros de salud y oficinas de farmacia en aras de una mejor atención a los pacientes.     ABSTRACT   OBJECTIVE To assess the performance and degree of satisfaction of the healthcare professionals involved in a cooperation programme between Daroca Health Centre and Community Pharmaceuticals in a Basic Health Area (DarocaFarmacias) in Madrid, Spain.   METHODS Thirty-six community pharmacies and 81 health care professionals of the Daroca Health Centre took part in this study, affecting an urban area of 53600 patients. Communications between Health Centre and Community Pharmacies were done by email and direct phone. Researching, clinical sessions and educational activities were collaboratively carried out. Different aspects of the project were analysed, such as communication, interprofessional relationship, training sessions and global satisfaction. With this aim, a blinded survey was submitted to the DarocaFarmacias members.   RESULTS Sixty-seven health professionals took part (32 community pharmacists, 21 physicians and 12 nurses) in the study. Among them, 95.5% are of the opinion that professional relationship improved and 82% agreed for a better-quality communication between health professionals. Most of the community pharmacists (43.7%) considered telephone as the preferred channel of communication (92% problem solving), followed by e-mail (31.2%), although half of them (53.1%) visited the Health Centre to solve a relevant question. Eighty-two out of 100 attended to the clinical sessions and educational activities, being considered as beneficial by 92.5% of them. Over nine out of ten (91%) of the health professionals were quite or very satisfied with DarocaFarmacias project.   CONCLUSION DarocaFarmacias is a positive experience of collaboration between Health Centre and community pharmacies in pursuit of a better attendance of the patients

Fomentando la cultura del emprendimiento en los niños De 5 a 12 años en el asentamiento humano Torres de San Borja

RESUMEN “Fomentando la cultura del emprendimiento en los niños” es un proyecto elaborado por los alumnos de la Universidad Privada del Norte, como parte de las actividades que lleva realizando el grupo UNIONES, a través del curso de Gestión Estratégica de la Responsabilidad Social Empresarial. Dicho proyecto se llevó a cabo en el Asentamiento Humano Las Torres de San Borja, en el Local Comunal de esta zona para ser más exacto, y tuvo como finalidad fomentar la cultura del emprendimiento en niños de 5 a 12 años, mediante la ejecución de un curso compuesto por 3 talleres, que se llevaron a cabo entre el 6 de Mayo y el 3 de Junio, en los cuales se les enseñó a los asistentes a elaborar alimentos sencillos que pudiesen preparar en sus hogares, para posteriormente, ofrecerlos al público, brindándoles de esta manera la oportunidad de iniciar su propio negocio y puedan así generar ingresos para sus hogares. El proceso por el cual pasó el proyecto, constó de varias etapas, iniciando con la planeación, en la que se determinaron las actividades que se iban a llevar a cabo en la comunidad y con qué miembros se iba a trabajar, siguiendo con la ejecución, donde se pusieron en práctica dichas actividades para cumplir con los objetivos del proyecto que se había planteado, y finalizando con la obtención de los resultados que se lograron al interactuar con el público objetivo, mediante el uso de herramientas metodológicas como encuestas y mediante el diálogo y la observación. Del mismo modo, se identificaron problemas que se presentaron en cada sesión y se recolectaron pruebas físicas, como fotografías y videos, donde se evidencia cada una de las tareas que se ejecutaron. De esta manera, fue posible determinar las conclusiones principales que se extrajeron de este proyecto y plantear recomendaciones para cualquier actividad futura de este tipo. PALABRAS CLAVES: Emprendimiento, proyecto, talleres, alimentos, proceso, etapas, planeación, ejecución, herramientas metodológicas.ABSTRACT "Promoting the culture of entrepreneurship in children" is a project developed by the students of the Private University of the North, as part of the activities carried out by the group “UNIONES”, through the course of Strategic Management of Corporate Social Responsibility. This project was realized in the Human Settlement “Las Torres de San Borja”, in the “Local Comunal” of this área, to be more exact, and had the purpose of promoting the culture of entrepreneurship in children from 5 to 12 years old, through the execution of a course composed of 3 workshops, which took place between May 6 and June 3, in which the attendees were taught to prepare simple foods that they could prepare in their homes, to later offer them to the public, providing them, in this way, the opportunity to start their own business and thus be able to generate income for their homes. The process by which the project went through, consisted of several stages, beginning with planning, in which the activities that were going to be executed in the community were determined and what members we were going to work with, following the execution, where these activities were put into practice to meet the objectives of the project that had been proposed, and ending with obtaining of the results achieved when interacting with the target audience, through the use of methodological tools as surveys and through dialogue and observation. In the same way, problems that were presented in each sesión were identified and physical evidence was collected, such as photographs and videos, where each of the tasks that were performed was evidenced. In this way, it was possible to determine the main conclusions drawn from this project and make recommendations for any future activity of this type. KEYWORDS: Entrepreneurship, project, workshops, foods, process, stages, planning, execution, methodological tools

BDNF as a potential mediator between childhood BPA exposure and behavioral function in adolescent boys from the INMA-Granada cohort

This research would not have been achieved without the selfless col-laboration of the INMA-Granada boys and families who took part in the study. Vicente Mustieles, Alicia Olivas-Martinez and Shereen Cynthia D'Cruz were under contract within the HBM4EU project. Additionally, we acknowledge the Biomedical Research Networking Center-CIBER de Epidemiologia y Salud Publica (CIBERESP) , and the Instituto de Salud Carlos III (ISCIII) (FIS-PI16/01820, FIS-PI16/01858, FIS-PI17/01526, and FIS-PI20/01568) . The authors also thank the ISCIII and "Fondo Europeo de Desarrollo Regional" (ISCIII/FEDER) for the Miguel Servet Type I Program granted to C. Freire (grant no. MS16/00085) , the Sara Borrell postdoctoral research contract granted to F. Vela-Soria (grant no. CD17/00212) , and the Spanish Ministry of Education for the predoctoral fellowships (FPU) granted to A. Rodriguez-Carrillo (FPU 16/03011) and to I. Reina-Perez (FPU 17/01848) . Dr. JP Arrebola is under contract within the Ramon y Cajal Program (RYC-2016-20155, from Ministerio de Economia, Industria y Competitividad, Spain) . The authors also ac-knowledge the contribution of the Pediatric Unit of San Cecilio University Hospital of Granada (recruitment and clinical evaluation) , Marina Molina (field work and biospecimen processing) , Raquel Quesada and Beatriz Suarez (chemical exposure data) and Mario Murcia (data curation) , as well as the Human Genotyping Laboratory at the Spanish National Cancer Research Centre, CeGen-PRB3, which is sup-ported by grant no. PT17/0019, of the PE I + D + i 2013-2016, funded by Instituto de Salud Carlos III (ISCIII) and ERDF. This article will be part of the doctoral thesis developed by Andrea Rodriguez-Carrillo in the context of the "Clinical Medicine and Public Health Program" of the University of Granada (Spain) .This study was supported in part by the European Union's Horizon 2020 research and innovation program HBM4EU [Grant Agreement No. 733032], Biomedical Research Networking Center-CIBER de Epidemiología y Salud Pública (CIBERESP), and the Instituto de Salud Carlos III (ISCIII) [Grant no. CP16/00085 and FIS-PI17/01526]. The funders had no role in the study design, data collection or analysis, decision to publish, or preparation of the manuscript. Funding for open access charge: Universidad de Granada / CBUA.Background: Bisphenol A (BPA) exposure has been linked to altered behavior in children. Within the European Human Biomonitoring Initiative (HBM4EU), an adverse outcome pathway (AOP) network was constructed supporting the mechanistic link between BPA exposure and brain-derived neurotrophic factor (BDNF). Objective: To test this toxicologically-based hypothesis in the prospective INMA-Granada birth cohort (Spain). Methods: BPA concentrations were quantified by LC-MS/MS in spot urine samples from boys aged 9-11 years, normalized by creatinine and log-2 transformed. At adolescence (15-17 years), blood and urine specimens were collected, and serum and urinary BDNF protein levels were measured using immunoassays. DNA methylation levels at 6 CpGs in Exon IV of the BDNF gene were also assessed in peripheral blood using bisulfite-pyrosequencing. Adolescent's behavior was parent-rated using the Child Behavior Checklist (CBCL/6-18) in 148 boys. Adjusted linear regression and mediation models were fit. Results: Childhood urinary BPA concentrations were longitudinally and positively associated with thought problems (beta = 0.76; 95% CI: 0.02, 1.49) and somatic complaints (beta = 0.80; 95% CI: -0.16, 1.75) at adolescence. BPA concentrations were positively associated with BDNF DNA methylation at CpG6 (beta = 0.21; 95% CI: 0.06, 0.36) and mean CpG methylation (beta = 0.10; 95% CI: 0.01, 0.18), but not with total serum or urinary BDNF protein levels. When independent variables were categorized in tertiles, positive dose-response associations were observed between BPA-thought problems (p-trend = 0.08), BPA-CpG6 (p-trend <_ 0.01), and CpG6-thought problems (p-trend <_ 0.01). A significant mediated effect by CpG6 DNA methylation was observed (beta = 0.23; 95% CI: 0.01, 0.57), accounting for up to 34% of the BPA-thought problems association. Conclusions: In line with toxicological studies, BPA exposure was longitudinally associated with increased BDNF DNA methylation, supporting the biological plausibility of BPA-behavior relationships previously described in the epidemiological literature. Given its novelty and preliminary nature, this effect biomarker approach should be replicated in larger birth cohorts.Instituto de Salud Carlos III"Fondo Europeo de Desarrollo Regional" (ISCIII/FEDER) MS16/00085Sara Borrell postdoctoral research contract grant CD17/00212Spanish Government FPU 16/03011- FPU 17/01848Ramon y Cajal Program (Ministerio de Economia, Industria y Competitividad, Spain) RYC-2016-20155Human Genotyping Laboratory at the Spanish National Cancer Research Centre, CeGen-PRB3 PT17/0019Instituto de Salud Carlos III European CommissionEuropean Commissio