A novel programmable lysozyme-based lysis system in Pseudomonas putida for biopolymer production

Indexación: Scopus; Web of Science.Cell lysis is crucial for the microbial production of industrial fatty acids, proteins, biofuels, and biopolymers. In this work, we developed a novel programmable lysis system based on the heterologous expression of lysozyme. The inducible lytic system was tested in two Gram-negative bacterial strains, namely Escherichia coli and Pseudomonas putida KT2440. Before induction, the lytic system did not significantly arrest essential physiological parameters in the recombinant E. coli (ECPi) and P. putida (JBOi) strain such as specific growth rate and biomass yield under standard growth conditions. A different scenario was observed in the recombinant JBOi strain when subjected to PHA-producing conditions, where biomass production was reduced by 25% but the mcl-PHA content was maintained at about 30% of the cell dry weight. Importantly, the genetic construct worked well under PHA-producing conditions (nitrogen-limiting phase), where more than 95% of the cell population presented membrane disruption 16 h post induction, with 75% of the total synthesized biopolymer recovered at the end of the fermentation period. In conclusion, this new lysis system circumvents traditional, costly mechanical and enzymatic cell-disrupting procedures.https://www.nature.com/articles/s41598-017-04741-2.pd

Searching for GC-like abundance patterns in young massive clusters II. - Results from the Antennae galaxies

The presence of multiple populations (MPs) with distinctive light element abundances is a widespread phenomenon in clusters older than 6 Gyr. Clusters with masses, luminosities, and sizes comparable to those of ancient globulars are still forming today. Nonetheless, the presence of light element variations has been poorly investigated in such young systems, even if the knowledge of the age at which this phenomenon develops is crucial for theoretical models on MPs. We use J-band integrated spectra of three young (7-40 Myr) clusters in NGC 4038 to look for Al variations indicative of MPs. Assuming that the large majority (>70%) of stars are characterised by high Al content - as observed in Galactic clusters with comparable mass; we find that none of the studied clusters show significant Al variations. Small Al spreads have been measured in all the six young clusters observed in the near-infrared. While it is unlikely that young clusters only show low Al whereas old ones display different levels of Al variations; this suggests the possibility that MPs are not present at such young ages at least among the high-mass stellar component. Alternatively, the fraction of stars with field-like chemistry could be extremely large, mimicking low Al abundances in the integrated spectrum. Finally, since the near-infrared stellar continuum of young clusters is almost entirely due to luminous red supergiants, we can also speculate that MPs only manifest themselves in low mass stars due to some evolutionary mechanism

A Mean-Field Theory for Coarsening Faceted Surfaces

A mean-field theory is developed for the scale-invariant length distributions observed during the coarsening of one-dimensional faceted surfaces. This theory closely follows the Lifshitz-Slyozov-Wagner theory of Ostwald ripening in two-phase systems [1-3], but the mechanism of coarsening in faceted surfaces requires the addition of convolution terms recalling the work of Smoluchowski [4] and Schumann [5] on coalescence. The model is solved by the exponential distribution, but agreement with experiment is limited by the assumption that neighboring facet lengths are uncorrelated. However, the method concisely describes the essential processes operating in the scaling state, illuminates a clear path for future refinement, and offers a framework for the investigation of faceted surfaces evolving under arbitrary dynamics. [1] I. Lifshitz, V. Slezov, Soviet Physics JETP 38 (1959) 331-339. [2] I. Lifshitz, V. Slyozov, J. Phys. Chem. Solids 19 (1961) 35-50. [3] C. Wagner, Elektrochemie 65 (1961) 581-591. [4] M. von Smoluchowski, Physikalische Zeitschrift 17 (1916) 557-571. [5] T. Schumann, J. Roy. Met. Soc. 66 (1940) 195-207

A spatially-structured PCG method for content diversity in a Physics-based simulation game

This paper presents a spatially-structured evolutionary algorithm (EA) to procedurally generate game maps of di ferent levels of di ficulty to be solved, in Gravityvolve!, a physics-based simulation videogame that we have implemented and which is inspired by the n- body problem, a classical problem in the fi eld of physics and mathematics. The proposal consists of a steady-state EA whose population is partitioned into three groups according to the di ficulty of the generated content (hard, medium or easy) which can be easily adapted to handle the automatic creation of content of diverse nature in other games. In addition, we present three fitness functions, based on multiple criteria (i.e:, intersections, gravitational acceleration and simulations), that were used experimentally to conduct the search process for creating a database of maps with di ferent di ficulty in Gravityvolve!.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Splitting hairs of the three charge black hole

We construct the large radius limit of the metric of three charge supertubes and three charge BPS black rings by using the fact that supertubes preserve the same supersymmetries as their component branes. Our solutions reproduce a few of the properties of three charge supertubes found recently using the Born Infeld description. Moreover, we find that these solutions pass a number of rather nontrivial tests which they should pass if they are to describe some of the hair of three charge black holes and three charge black rings.Comment: 15 pages, LaTeX, v2 minor correction

Crossover of interface growth dynamics during corrosion and passivation

We study a model of corrosion and passivation of a metalic surface in contact with a solution using scaling arguments and simulation. The passive layer is porous so that the metal surface is in contact with the solution. The volume excess of the products may suppress the access of the solution to the metal surface, but it is then restored by a diffusion mechanism. A metalic site in contact with the solution or with the porous layer can be passivated with rate p and volume excess diffuses with rate D. At small times, the corrosion front linearly grows in time, but the growth velocity shows a t^{-1/2} decrease after a crossover time of order t_c ~ D/p^2, where the average front height is of order h_c ~ D/p. A universal scaling relation between h/h_c and t/t_c is proposed and confirmed by simulation for 0.00005 <= p <= 0.5 in square lattices. The roughness of the corrosion front shows a crossover from Kardar-Parisi-Zhang scaling to Laplacian growth (diffusion-limited erosion - DLE) at t_c. The amplitudes of roughness scaling are obtained by the same kind of arguments as previously applied to other competitive growth models. The simulation results confirm their validity. Since the proposed model captures the essential ingredients of different corrosion processes, we also expect these universal features to appear in real systems.Comment: 17 pages, including 7 figures; submitted articl

Chemical inhomogeneities amongst first population stars in globular clusters Evidence for He variations

Spreads in light element abundances among stars (also known as multiple populations) are observed in nearly all globular clusters. One way to map such chemical variations using high-precision photometry is to employ a suitable combination of stellar magnitudes in the F275W, F336W, F438W, and F814W filters (called the “chromosome map”), to maximise the separation between the different multiple populations. For each individual cluster its chromosome map separates the first population (with metal abundance patterns typical of field halo stars) from the second population (which displays distinctive abundance variations among a specific group of light elements). Surprisingly, the distribution of first population stars in chromosome maps of several but not all clusters has been found to be more extended than expected from purely observational errors, suggesting a chemically inhomogeneous origin. We consider here three clusters with similar metallicity ([Fe/H] ~ −1.3) and different chromosome maps, namely NGC 288, M 3, and NGC 2808, and argue that the first population extended distribution (as observed in two of these clusters) is due to spreads of the initial helium abundance and possibly a small range of nitrogen abundances as well. The presence of a range of initial He and N abundances amongst stars traditionally thought to have homogeneous composition, and that these spreads appear only in some clusters, challenges the scenarios put forward so far to explain the multiple population phenomenon

Young stellar populations in type II quasars: timing the onset of star formation and nuclear activity

Despite the emerging morphological evidence that luminous quasar-like active galactic nuclei (AGN) are triggered in galaxy mergers, the natures of the triggering mergers and the order of events in the triggering sequence remain uncertain. In this work, we present a detailed study of the stellar populations of the host galaxies of 21 type II quasars, with the aim of understanding the sequence of events between the onset of the merger, the triggering of the associated starburst and the initiation of the quasar activity. To this end, we model highquality, wide spectral coverage, intermediate-resolution optical spectra of the type II quasars. We find that of the 21 objects, the higher order Balmer absorption lines, characteristic of young stellar populations (YSPs), are directly detected in ∼62 per cent of the sample. We also fit these spectra using a number of combinations of stellar and/or power-law components, representative of viable formation histories, as well as including the possibility of scattered AGN light. We find that ∼90 per cent of the type II quasar host galaxies require the inclusion of a YSP to adequately model their spectra, whilst 71 per cent of the sample require the inclusion of a YSP with age <100 Myr. Since the ages of the YSP in most type II quasar host galaxies are comparable with the expected lifetimes of the AGN activity, these results provide strong evidence that the quasars are triggered close to the peaks of the merger-induced starbursts