Time series of high resolution spectra of SN 2014J observed with the TIGRE telescope

We present a time series of high resolution spectra of the Type Ia supernova 2014J, which exploded in the nearby galaxy M82. The spectra were obtained with the HEROS echelle spectrograph installed at the 1.2 m TIGRE telescope. We present a series of 33 spectra with a resolution of R = 20, 000, which covers the important bright phases in the evolution of SN 2014J during the period from January 24 to April 1 of 2014. The spectral evolution of SN 2014J is derived empirically. The expansion velocities of the Si II P-Cygni features were measured and show the expected decreasing behaviour, beginning with a high velocity of 14,000 km/s on January 24. The Ca II infrared triplet feature shows a high velocity component with expansion velocities of > 20, 000 km/s during the early evolution apart from the normal component showing similar velocities as Si II. Further broad P-Cygni profiles are exhibited by the principal lines of Ca II, Mg II and Fe II. The TIGRE SN 2014J spectra also resolve several very sharp Na I D doublet absorption components. Our analysis suggests interesting substructures in the interstellar medium of the host galaxy M82, as well as in our Milky Way, confirming other work on this SN. We were able to identify the interstellar absorption of M82 in the lines of Ca II H & K at 3933 and 3968 A as well as K I at 7664 and 7698 A. Furthermore, we confirm several Diffuse Interstellar Bands, at wavelengths of 6196, 6283, 6376, 6379 and 6613 A and give their measured equivalent widths.Comment: 11 pages, 10 figures, accepted for publication in MNRA

Optical and X-ray properties of the RIXOS AGN: II - Emission lines

We present the optical and UV emission line properties of 160 X-ray selected AGN taken from the RIXOS survey (including Halpha, Hbeta, [OIII]5007, MgII2798 and CIII]1909). This sample is believed to contain a mixture of absorbed and unabsorbed objects, with column densities up to 4e21 cm-2. Although the distribution of the [OIII] EW for the RIXOS AGN is typical of optically selected samples, the Balmer line EWs are relatively low. This is consistent with the presence of a dust absorber between the broad and narrow line regions (eg. a molecular torus), and intrinsically weak optical line emission. We find Baldwin effects in CIII] and MgII, and a positive response of the MgII line to its ionizing continuum. There is a strong correlation between the EW and FWHM of MgII, which may be similar to that seen in other samples for Hbeta. We demonstrate that this is consistent with models which suggest two line-emitting zones, a `very broad line region' (VBLR) and an `intermediate line region' (ILR). The correlation between EW and FWHM in MgII may be a physical characteristic of the ILR or it may reflect a geometric dependence. We found no correlation between the Hbeta FWHM and the slope of the X-ray spectrum, however this may be due to the effects of dust absorption which suppresses the broad Hbeta component, masking any relationship. The Halpha FWHM does tend to be narrow when alpha_X is soft, and broadens as alpha_X hardens, although the formal probability for this correlation is low (91 per cent). If the distribution of alpha_X in the RIXOS sample reflects the level of intrinsic absorption in these AGN, the data suggest a possible link between the velocity of the Balmer line-emitting region and the amount of absorbing material beyond.Comment: 29 pages, 14 figures, to be published in Monthly Notices of the Royal Astronomical Society. Also available from http://www.mssl.ucl.ac.uk/www_astro/preprints/preprints.htm

Galaxy-wide radio-induced feedback in a radio-quiet quasar

We report the discovery of a radio-quiet type 2 quasar (SDSS J165315.06+234943.0 nicknamed the ‘Beetle’ at z = 0.103) with unambiguous evidence for active galactic nucleus (AGN) radio-induced feedback acting across a total extension of ∼46 kpc and up to ∼26 kpc from the AGN. To the best of our knowledge, this is the first radio-quiet system where radio-induced feedback has been securely identified at ≫several kpc from the AGN. The morphological, ionization and kinematic properties of the extended ionized gas are correlated with the radio structures. We find along the radio axis (a) enhancement of the optical line emission at the location of the radio hotspots (b) turbulent gas kinematics (FWHM ∼ 380–470 km s−1) across the entire spatial range circumscribed by them (c) ionization minima for the turbulent gas at the location of the hot spots, (d) high temperature Te ≳ 1.9 × 104 K at the NE hotspot. Turbulent gas is also found far from the radio axis, ∼25 kpc in the perpendicular direction. We propose a scenario in which the radio structures have perforated the interstellar medium of the galaxy and escaped into the circumgalactic medium. While advancing, they have interacted with in situ gas modifying its properties. Our results show that jets of modest power can be the dominant feedback mechanism acting across huge volumes in radio-quiet systems, including highly accreting luminous AGNs, where radiative mode feedback may be expected

The ELAIS Deep X-ray Survey

We present initial follow-up results of the ELAIS Deep X-ray Survey which is being undertaken with the Chandra and XMM-Newton Observatories. 235 X-ray sources are detected in our two 75 ks ACIS-I observations in the well-studied ELAIS N1 and N2 areas. 90% of the X-ray sources are identified optically to R=26 with a median magnitude of R=24. We show that objects which are unresolved optically (i.e. quasars) follow a correlation between their optical and X-ray fluxes, whereas galaxies do not. We also find that the quasars with fainter optical counterparts have harder X-ray spectra, consistent with absorption at both wavebands. Initial spectroscopic follow-up has revealed a large fraction of high-luminosity Type 2 quasars. The prospects for studying the evolution of the host galaxies of X-ray selected Type 2 AGN are considered.Comment: 9 pages, 5 figures, To appear in Proceedings of XXI Moriond Conference: "Galaxy Clusters and the High Redshift Universe Observed in X-rays", edited by D. Neumann, F.Durret, & J. Tran Thanh Va

Spain's Budget Neglects Research

Letter.-- Carlos Fenollosa et al.Peer Reviewe

Pranlukast Antagonizes CD49f and Reduces Sternness in Triple-Negative Breast Cancer Cells

Introduction: Cancer stem cells (CSCs) drive the initiation, maintenance, and therapy response of breast tumors. CD49f is expressed in breast CSCs and functions in the maintenance of stemness. Thus, blockade of CD49f is a potential therapeutic approach for targeting breast CSCs. In the present study, we aimed to repurpose drugs as CD49f antagonists. Materials and Methods: We performed consensus molecular docking using a subdomain of CD49f that is critical for heterodimerization and a collection of pharmochemicals clini-cally tested. Molecular dynamics simulations were employed to further characterize drug-target binding. Using MDA-MB-231 cells, we evaluated the effects of potential CD49f antagonists on 1) cell adhesion to laminin; 2) mammosphere formation; and 3) cell viability. We analyzed the effects of the drug with better CSC-selectivity on the activation of CD49f-downstream signaling by Western blot (WB) and co-immunoprecipitation. Expressions of the stem cell markers CD44 and SOX2 were analyzed by flow cytometry and WB, respectively. Transactivation of SOX2 promoter was evaluated by luciferase reporter assays. Changes in the number of CSCs were assessed by limiting-dilution xenotransplantation. Results: Pranlukast, a drug used to treat asthma, bound to CD49f in silico and inhibited the adhesion of CD49f+ MDA-MB-231 cells to laminin, indicating that it antagonizes CD49f-containing integrins. Molecular dynamics analysis showed that pranlukast binding induces con-formational changes in CD49f that affect its interaction with β1-integrin subunit and constrained the conformational dynamics of the heterodimer. Pranlukast decreased the clonogenicity of breast cancer cells on mammosphere formation assay but had no impact on the viability of bulk tumor cells. Brief exposure of MDA-MB-231 cells to pranlukast altered CD49f-dependent signaling, reducing focal adhesion kinase (FAK) and phosphatidylinositol 3-kinase (PI3K) activation. Further, pranlukast-treated cells showed decreased CD44 and SOX2 expression, SOX2 promoter transacti-vation, and in vivo tumorigenicity, supporting that this drug reduces the frequency of CSC. Conclusion: Our results support the function of pranlukast as a CD49f antagonist that reduces the CSC population in triple-negative breast cancer cells. The pharmacokinetics and toxicology of this drug have already been established, rendering a potential adjuvant therapy for breast cancer patients

Human prophylactic vaccine adjuvants and their determinant role in new vaccine formulations

Adjuvants have been considered for a long time to be an accessory and empirical component of vaccine formulations. However, accumulating evidence of their crucial role in initiating and directing the immune response has increased our awareness of the importance of adjuvant research in the past decade. Nevertheless, the importance of adjuvants still is not fully realized by many researchers working in the vaccine field, who are involved mostly in the search for better target antigens. The choice of a proper adjuvant can be determinant for obtaining the best results for a given vaccine candidate, but it is restricted due to intellectual property and know-how issues. Consequently, in most cases the selected adjuvant continues to be the aluminum salt, which has a record of safety, but predominantly constitutes a delivery system (DS). Ideally, new strategies should combine immune potentiators (IP) and DS by mixing both compounds or by obtaining structures that contain both IP and DS. In addition, the term immune polarizer has been introduced as an essential concept in the vaccine design strategies. Here, we review the theme, with emphasis on the discussion of the few licensed new adjuvants, the need for safe mucosal adjuvants and the adjuvant/immunopotentiating activity of conjugation. A summary of toxicology and regulatory issues will also be discussed, and the Finlay Adjuvant Platform is briefly summarized

Testing the theory of immune selection in cancers that break the rules of transplantation

Modification of cancer cells likely to reduce their immunogenicity, including loss or down-regulation of MHC molecules, is now well documented and has become the main support for the concept of immune surveillance. The evidence that these modifications, in fact, result from selection by the immune system is less clear, since the possibility that they may result from reorganized metabolism associated with proliferation or from cell de-differentiation remains. Here, we (a) survey old and new transplantation experiments that test the possibility of selection and (b) survey how transmissible tumours of dogs and Tasmanian devils provide naturally evolved tests of immune surveillance