EFFECT OF SLEEP DEPRIVATION ON REMINISCENCE AND APPREHENSION BEHAVIOR IN WISTAR ALBINO RATS

Objective: The aim of the present study is to investigate the effect of paradoxical sleep deprivation (SD) on learning and memory impairment and anxiety-like behavior in female Wistar albino rats. Methods: Eight-arm radial maze, open-field test, and light and dark test were used to assess the animals learning and memory and anxiety-like behavior. Results: SD associated with weaker learning and memory and increased anxiety- and depressive-like behavior in animals. Conclusion: Animals were exposed to SD showed learning and memory impairment and also exhibited increased anxiety- and depressive-like behavior when compared to control animals

Effect of Laser as An Adjunct to Nonsurgical Periodontal Treatment on Serum Inflammatory Markers in Patients with Chronic Periodontitis: A Clinico Biochemical Study

Background: The most common oral disease in response to the chronic infection caused by different periodontopathogenic bacteria is periodontitis resulting from inflammation of supporting structures of teeth. It has been shown that nonsurgical periodontal treatment (SRP) result in significant clinical improvements, but they do not completely remove periodontopathogens, especially in deep periodontal Adjunctive therapies improve tissue healing through detoxification and bactericidal effects. In recent years, adjunctive use of lasers has shown to be a promising modality to increase the effectiveness of nonsurgical periodontal treatment. The objective of the study is to evaluate the efficacy of diode laser in addition to scaling and root planning on periodontal parameters and CRP levels in chronic periodontitis. Materials and Methods: 24 chronic periodontitis patients were grouped as group 1(n=12) and group2(n=12) where in group 1 scaling and root planing was done and group 2 scaling and root planning with subgingival debridement using diode laser was done. The probing pocket depth, gingival index, plaque index, C-reactive protein, total leucocyte counts and erythrocyte sedimentation rate was recorded at baseline and after 1 month. Results: There was statistically significant reduction in probing pocket depth, CRP, ESR and TLC in both groups from baseline to 1 month. Conclusion: Both treatment groups showed reduction in the periodontal parameters and biomarkers. Adjunctive use of laser had same clinical benefits as to scaling and root planing alone

A RANDOMIZED CASE–CONTROL PILOT STUDY ON THE NEUROCHEMICAL BASIS OF PAIN MODULATION IN PATIENTS WITH MIGRAINE, WHO PRACTICED INTEGRATED AMRITA MEDITATION TECHNIQUE

Objective: The objective of the study was to determine the changes in duration and intensity of headache and associated changes in the plasma levels of neurochemicals, serotonin, glutamate, vasoactive intestinal peptide (VIP), and nitric oxide (NO) in patients with migraine after 6 months of regular practice of integrated Amrita meditation (IAM). Methods: Sixteen patients aged 18–50 with migraine were randomly assigned to 2 groups, one with standard medical care and IAM, and the other only standard medical care. Data were collected before IAM, after 3 and 6 months of IAM practice Results: After 6 months, a significant decrease in the duration of headache from 2.4±0.54 to 1.4±0.54 (p=0.034) hours and intensity of pain from 3.6±0.54 to 2.6±0.89, (p=0.035) was seen in patients who practiced IAM. Plasma levels of serotonin within the IAM group increased (47.29±26.85 to 53.85±29.73ng/ml), where as there was decrease in glutamate (38.47±8.2 to 29.68±12.57μg/ml), VIP (28.01±13.64 to 22.23±7.79pg/ml) and NO levels (642.26±167.42 to 423.18±97.96μmol/L). A correlating trend was seen in comparison with control group after 6 months showing a statistically significant difference in plasma Serotonin (p value 0.007) and NO (p value-0.023) levels. Discussion: The results of our study have been discussed with other migraine and meditation-related studies. Conclusion: Regular practice of IAM reduces the intensity and duration of headache in migraine patients and is associated with alterations in the neurotransmitter levels

Induction Heating for Variably Sized Ferrous and Non-Ferrous Materials through Load Modulation

Induction heating (IH) is a process of heating the electrically conducting materials especially ferromagnetic materials with the help of electromagnetic induction through generating heat in an object by eddy currents. A well-entrenched way of IH is to design a heating system pertaining to the usage of ferromagnetic materials such as stainless steel, iron, etc., which restricts the end user’s choice of using utensils made of ferromagnetic only. This research article proposes a new scheme of induction heating that is equally effective for heating ferromagnetic and non-ferromagnetic materials such as aluminium and copper. This is achieved by having a competent IH system that embodies a series resonant inverter and controller where a competent flexible load modulation (FLM) is deployed. FLM facilitates change in operating frequency in accordance with the type of material chosen for heating. The recent attempts by researchers on all metal IH have not addressed much on the variable shapes and sizes of the material, whereas this research attempts to address that issue as well. The proposed induction heating system is verified for a 2 kW system and is compatible with both industrial and domestic applications

A single-coil multi-tapped PDM-based induction heating system for domestic applications

The conventional heating system is inefficient as the major part of the heating coil lies out-side the vessel it is placed on. This research article proposes a new single-coil multi-tapped induction heating system. This novel induction heating system is facilitated by a half-bridge resonant converter controlled by zero-voltage switching (ZVS). The multi-tapping winding system ensures an effective heat transfer between the coil and the working vessel with the windings of the induction coil segmented to an equivalent size of the vessel. The pulse density modulation (PDM) scheme employed here as the control proves to be the most versatile one. The whole system is duly simulated for an 850 W IH setup in MATLAB Simulink and implemented as a hardware prototype using a half-bridge resonant converter. The control pulses are developed through the PDM in a PIC16F877A controller. The simulation and experimental results prove the credibility of the proposed induction heating (IH) scheme, and during heavy loading conditions, it outperforms the single-coil IH system by gaining an efficiency of 89.29

The RNA-binding protein Sam68 regulates expression and transcription function of the androgen receptor splice variant AR-V7.

Castration-resistant (CR) prostate cancer (PCa) partly arises due to persistence of androgen receptor (AR) transcriptional activity in the absence of cognate ligand. An emerging mechanism underlying the CRPCa phenotype and predicting response to therapy is the expression of the constitutively-active AR-V7 splice variant generated by AR cryptic exon 3b inclusion. Here, we explore the role of the RNA-binding protein (RBP) Sam68 (encoded by KHDRBS1), which is over-expressed in clinical PCa, on AR-V7 expression and transcription function. Using a minigene reporter, we show that Sam68 controls expression of exon 3b resulting in an increase in endogenous AR-V7 mRNA and protein expression in RNA-binding-dependent manner. We identify a novel protein-protein interaction between Sam68 and AR-V7 mediated by a common domain shared with full-length AR, and observe these proteins in the cell nucleoplasm. Using a luciferase reporter, we demonstrate that Sam68 co-activates ligand-independent AR-V7 transcriptional activity in an RNA-binding-independent manner, and controls expression of the endogenous AR-V7-specific gene target UBE2C. Our data suggest that Sam68 has separable effects on the regulation of AR-V7 expression and transcriptional activity, through its RNA-binding capacity. Sam68 and other RBPs may control expression of AR-V7 and other splice variants as well as their downstream functions in CRPCa

Protocol for the ProFHER (PROximal Fracture of the Humerus: Evaluation by Randomisation) trial: a pragmatic multi-centre randomised controlled trial of surgical versus non-surgical treatment for proximal fracture of the humerus in adults

<p>Abstract</p> <p>Background</p> <p>Proximal humeral fractures, which occur mainly in older adults, account for approximately 4 to 5% of all fractures. Approximately 40% of these fractures are displaced fractures involving the surgical neck. Management of this group of fractures is often challenging and the outcome is frequently unsatisfactory. In particular it is not clear whether surgery gives better outcomes than non-surgical management. Currently there is much variation in the use of surgery and a lack of good quality evidence to inform this decision.</p> <p>Methods/Design</p> <p>We aim to undertake a pragmatic UK-based multi-centre randomised controlled trial evaluating the effectiveness and cost-effectiveness of surgical versus standard non-surgical treatment for adults with an acute closed displaced fracture of the proximal humerus with involvement of the surgical neck. The choice of surgical intervention is left to the surgeon, who must use techniques that they are fully experienced with. This will avoid 'learning curve' problems. We will promote good standards of non-surgical care, similarly insisting on care-provider competence, and emphasize the need for comparable provision of rehabilitation for both groups of patients.</p> <p>We aim to recruit 250 patients from a minimum of 18 NHS trauma centres throughout the UK. These patients will be followed-up for 2 years. The primary outcome is the Oxford Shoulder Score, which will be collected via questionnaires completed by the trial participants at 6, 12 and 24 months. This is a 12-item condition-specific questionnaire providing a total score based on the person's subjective assessment of pain and activities of daily living impairment. We will also collect data for other outcomes, including general health measures and complications, and for an economic evaluation. Additionally, we plan a systematic collection of reasons for non-inclusion of eligible patients who were not recruited into the trial, and their baseline characteristics, treatment preferences and intended treatment.</p> <p>Discussion</p> <p>This article presents the protocol for a multi-centre randomised controlled trial. It gives extensive details of, and the basis for, the chosen methods, and describes the key measures taken to avoid bias and to ensure validity.</p> <p>Trial Registration</p> <p>Current Controlled Trials ISRCTN50850043</p

WNT signalling in prostate cancer

Genome sequencing and gene expression analyses of prostate tumours have highlighted the potential importance of genetic and epigenetic changes observed in WNT signalling pathway components in prostate tumours-particularly in the development of castration-resistant prostate cancer. WNT signalling is also important in the prostate tumour microenvironment, in which WNT proteins secreted by the tumour stroma promote resistance to therapy, and in prostate cancer stem or progenitor cells, in which WNT-β-catenin signals promote self-renewal or expansion. Preclinical studies have demonstrated the potential of inhibitors that target WNT receptor complexes at the cell membrane or that block the interaction of β-catenin with lymphoid enhancer-binding factor 1 and the androgen receptor, in preventing prostate cancer progression. Some WNT signalling inhibitors are in phase I trials, but they have yet to be tested in patients with prostate cancer