Self-assembly of glycoprotein nanostructured filaments for modulating extracellular networks at long range

The intriguing capability of branched glycoprotein filaments to change their hierarchical organization, mediated by external biophysical stimuli, continues to expand understanding of self-assembling strategies that can dynamically rearrange networks at long range. Previous research has explored the corresponding biological, physiological and genetic mechanisms, focusing on protein assemblies within a limited range of nanometric units. Using direct microscopy bio-imaging, we have determined the morpho-structural changes of self-assembled filament networks of the zona pellucida, revealing controlled levels of structured organizations to join distinct evolved stages of the oocyte (Immature, Mature, and Fertilized). This natural soft network reorganizes its corresponding hierarchical network to generate symmetric, asymmetric, and ultimately a state with the lowest asymmetry of the outer surface roughness, and internal pores reversibly changed from elliptical to circular configurations at the corresponding stages. These elusive morpho-structural changes are regulated by the nanostructured polymorphisms of the branched filaments by self-extension/-contraction/-bending processes, modulated by determinate theoretical angles among repetitive filament units. Controlling the nanoscale self-assembling properties by delivering a minimum number of activation bio-signals may be triggered by these specific nanostructured polymorphic organizations. Finally, this research aims to guide this soft biomaterial into a desired state to protect oocytes, eggs, and embryos during development, to favour/prevent the fertilization/polyspermy processes and eventually to impact interactions with bacteria/virus at multiscale levels.The intriguing capability of branched glycoprotein filaments to change their hierarchical organization, mediated by external biophysical stimuli, continues to expand understanding of self-assembling strategies that can dynamically rearrange networks at long range

Clues to the Formation of Liller 1 from Modeling Its Complex Star Formation History

Liller 1 and Terzan 5 are two massive systems in the Milky Way bulge hosting populations characterized by significantly different ages (Δt > 7-8 Gyr) and metallicities (Δ[Fe/H] ∼ 1 dex). Their origin is still strongly debated in the literature and all formation scenarios proposed so far require some level of fine-tuning. The detailed star formation histories of these systems may represent an important piece of information to assess their origin. Here we present the first attempt to perform such an analysis for Liller 1. The first key result we find is that Liller 1 has been forming stars over its entire lifetime. More specifically, three broad star formation episodes are clearly detected: (1) a dominant one, occurring some 12-13 Gyr ago with a tail extending for up to ∼3 Gyr; (2) an intermediate burst, between 6 and 9 Gyr ago; and (3) a recent one, occurring between 1 and 3 Gyr ago. The old population contributes to about 70% of the total stellar mass, and the remaining fraction is almost equally split between the intermediate and young populations. If we take these results at face value, they would suggest that this system unlikely formed through the merger between an old globular cluster and a giant molecular cloud, as recently proposed. On the contrary, our findings provide further support to the idea that Liller 1 is the surviving relic of a massive primordial structure that contributed to the Galactic bulge formation, similarly to the giant clumps observed in star-forming high-redshift galaxies

High-resolution Extinction Map in the Direction of the Strongly Obscured Bulge Fossil Fragment Liller 1*

We used optical images acquired with the Wide Field Camera of the Advanced Camera for Surveys on board the Hubble Space Telescope and near-infrared data from Gemini Multi-conjugate Adaptive Optics System (GeMS)/Gemini South Adaptive Optics Imager (GSAOI) to construct a high-resolution extinction map in the direction of the bulge stellar system Liller 1. In spite of its appearance of a globular cluster, Liller 1 has been recently found to harbor two stellar populations with remarkably different ages, and it is the second complex stellar system with similar properties (after Terzan 5) discovered in the bulge, thus defining a new class of objects: the Bulge Fossil Fragments. Because of its location in the inner bulge of the Milky Way, very close to the Galactic plane, Liller 1 is strongly affected by large and variable extinction. The simultaneous study of both the optical and the near-infrared color–magnitude diagrams revealed that the extinction coefficient RV in the direction of Liller 1 has a much smaller value than commonly assumed for diffuse interstellar medium (RV = 2.5, instead of 3.1), in agreement with previous findings along different light paths to the Galactic bulge. The derived differential reddening map has a spatial resolution ranging from 1'' to 3'' over a field of view of about 90'' × 90''. We found that the absorption clouds show patchy substructures with extinction variations as large as δE(B − V) ∼ 0.9 mag

Common genetic variants on chromosome 9p21 are associated with myocardial infarction and type 2 diabetes in an Italian population

<p>Abstract</p> <p>Background</p> <p>A genomic region on chromosome 9p21 has been identified as closely associated with increased susceptibility to coronary artery disease (CAD) and to type 2 diabetes (T2D) although the evidence suggests that the genetic variants within chromosome 9p21 that contribute to CAD are different from those that contribute to T2D.</p> <p>We carried out an association case-control study in an Italian population to test the association between two single nucleotide polymorphisms (SNPs) on the 9p21 locus, rs2891168 and rs10811661, previously reported by the PROCARDIS study, and respectively myocardial infarction (MI) and T2D. Our aim was to confirm the previous findings on a larger sample and to verify the independence of their susceptibility effects: rs2891168 associated with MI but not with T2D and rs10811661 associated with T2D but not with MI.</p> <p>Methods</p> <p>Genomic DNA samples of 2407 Italians with T2D (602 patients), who had had a recent MI (600), or had both diseases (600) and healthy controls (605) were genotyped for the two SNPs. The genotypes were determined by allelic discrimination using a fluorescent-based TaqMan assay.</p> <p>Results</p> <p>SNP rs2891168 was associated with MI, but not with T2D and the G-allele odds ratio (OR) was 1.20 (95% CI 1.02-1.41); SNP rs10811661 was associated with T2D, but not with MI, and the T-allele OR was 1.27 (95% CI 1.04-1.55). ORs estimates from the present study and the PROCARDIS study were pooled and confirmed the previous findings, with greater precision.</p> <p>Conclusions</p> <p>Our replication study showed that rs2891168 and rs10811661 are independently associated respectively with MI and T2D in an Italian population. Pooling our results with those reported by the PROCARDIS group, we also obtained a significant result of association with diabetes for rs10811661 in the European population.</p

Nuove ricerche sui mosaici funerari tardoantichi. I capisaldi cronologici

Presentazione di un ampio studio sulla cronologia dei mosaici funerari tardoantichi nel Mediterraneo

Remote monitoring system as a tool for calving management in Mediterranean Buffalo heifers (Bubalus bubalis)

Buffalo breeding is common in Southern Italy. Dystocia compromises dam's and newborn health and welfare. Difficult parturition could be solved through prompt calving assistance, even if the identification of the beginning of delivery is challenging. Herein, we aimed to evaluate a remote calving alarm system in 15 Mediterranean buffalo heifers. An intravaginal probe was placed close to the external cervical os once premonitory signs of delivery were observed. No vaginal discharge nor signs of discomfort were notified in the days following the insertion of the probe. Heifers calved from 48 to 72\ua0hr after the alarm was activated. The system correctly warned the farm personnel at the beginning of stage II of parturition, except for 2 cases. In the former, the intravaginal probe was expelled but the poor carrier network coverage negatively affected phone's signal quality; in the latter, recurrent vaginal prolapse was responsible for non-retention of the probe. Overall median expulsive phase was 68\ua0\ub1\ua08\ua0min, while the expulsion of a female calf took 54\ua0\ub1\ua022.0\ua0min and 90\ua0\ub1\ua034.0\ua0min in males, with significant difference (p\ua0=.02). Deliveries were homogeneously distributed across a 24-hr interval. No retention of foetal membranes nor metritis was identified at postpartum clinical examination. The calving alarm system used in this work was well tolerated in buffalo heifers. The introduction of smart technology in buffalo farming could contribute to the overall farm net return by reducing calf losses, especially for calves born from sexed-sorted semen, and by increasing animal welfare through quick resolution of dystocia. Further studies will be necessary to evaluate the net return in buffalo farms which will implement a remote calving alarm system on a wider population

Coinfection of the central nervous system by cytomegalovirus and herpes simplex virus type 1 or 2 in AIDS patients: autopsy study on 82 cases by immunohistochemistry and polymerase chain reaction

We evaluated the frequency and histopathological features of concomitant infections of the central nervous system (CNS) with cytomegalovirus (CMV) and herpes simplex viruses type 1 or 2 (HSV1/2) in a large series of patients who had died from AIDS. Eighty-two autopsy cases with a histological diagnosis of CMV necrotizing encephalitis were examined retrospectively. CMV and HSV1/2 were detected by immunohistochemistry (IHC) with poly- and monoclonal antibodies and by nested polymerase chain reaction (PCR) for HSV 1 and 2 on DNA extracted from paraffin blocks. PCR for a beta-globin genomic sequence was performed in all IHC-positive cases to verify the integrity of extracted DNA. Concomitant CMV/HSV infections were demonstrated by IHC in 13 cases (16%); using monoclonal antibodies, HSV1 was found in 9 cases and HSV2 in 4 cases. In half of the cases, HSV1- or HSV2-positive cells represented more than 25% of immunopositive CMV cells. In all 13 cases, double immunochemical staining showed cells containing both CMV and HSV antigens. PCR for HSV1 and 2 was positive in only 7 of 13 cases (5 HSV1 and 2 HSV2). In the remaining 6 negative cases PCR for beta-globin was also repeatedly negative. HSV1 or 2 infection can be demonstrated by IHC in a significant proportion of AIDS cases with necrotizing CMV encephalitis. Nested PCR for HSV1 and 2 on DNA extracted from formalin-fixed and paraffin-embedded autopsy tissues was positive in only slighty above 50% of IHC-positive cases

La basilica di Santa Croce. Nuovi contributi per Ravenna tardoantica

Raccolta di saggi dedicati al complesso di Santa Croce e al mausoleo di Galla Placidia in Ravenn