Fractal Exchange in a Cannibal Cosmology: Dynamics of Opposition and Amity in Amazonian Festivals

El presente artículo analiza un patrón de fiestas intercomunidades en el caso de un grupo nativo contemporáneo: los wari’ de Rondônia, Brasil. Los wari’ manejan sus relaciones con aliados y potenciales aliados en rituales estructurados alrededor de actos y símbolos de trasgresión, castigo, depredación y muerte. En un patrón social "fractal" —es decir, un patrón con una estructura básica que se repite en diferentes escalas— estos encuentros rituales intercomunidades son llevados a otras dimensiones en las relaciones humanas con el mundo espiritual, el mundo de los antepasados y los animales. Estos rituales constituyen un mediador clave de la reproducción social para el caso de las relaciones tradicionales entre las comunidades wari’ y de la reproducción biológica en las relaciones con las fuerzas espirituales que controlan la subsistencia y la mortalidad. Si las posibilidades simultáneas de cooperación y oposición se mantienen en tensión dinámica, este marco de relaciones intergrupales, concebidas como de rivalidad simbólica y "depredación" recíproca voluntaria, permite a las comunidades cultivar o poner fin, según sea el caso, las filiaciones políticas y de compromiso en respuesta a circunstancias cambiantes. Estos encuentros rituales tienen un papel importante en los intercambios de información y la definición y mantenimiento de las redes de afiliaciones o alianzas caracterizadas por su flexibilidad y la posibilidad de movilidad que, en el pasado, permitieron que los wari’ sobrellevaran las presiones históricas de violencia interétnica y las epidemias.This article examines a pattern of inter-community parties in a contemporary indigenous group, the Wari’ of Rondônia, Brazil. In rituals structured around acts and symbols of transgression, punishment, predation and death, Wari’ negotiate their relations with allies and potential allies. In a "fractal" pattern of sociality, this ritual encounter is replicated at other levels, in human relations with the spirit world, the world of ancestors and animals. These rituals are a key mediator of social reproduction (in relations among Wari’ communities) and biological reproduction (in relations with the spiritual forces that control aspects of subsistence and human death). Holding in tension simultaneous possibilities for cooperation and opposition, this framework for inter-group relations conceived as symbolic rivalry and voluntary reciprocal predation allows communities to cultivate and terminate political affiliations and commitments in response to changing circumstances. These ritual encounters play an important role in exchanges of information, and in defining and maintaining networks of flexible alliances and the possibility of mobility, which in the past helped Wari’ cope with historical pressures of inter-ethnic violence and epidemics

Affective lability and difficulties with regulation are differentially associated with amygdala and prefrontal response in women with Borderline Personality Disorder

The present neuroimaging study investigated two aspects of difficulties with emotion associated with Borderline Personality Disorder (BPD()): affective lability and difficulty regulating emotion. While these two characteristics have been previously linked to BPD symptomology, it remains unknown whether individual differences in affective lability and emotion regulation difficulties are subserved by distinct neural substrates within a BPD sample. To address this issue, sixty women diagnosed with BPD were scanned while completing a task that assessed baseline emotional reactivity as well as top-down emotion regulation. More affective instability, as measured by the Affective Lability Scale (ALS()), positively correlated with greater amygdala responses on trials assessing emotional reactivity. Greater difficulties with regulating emotion, as measured by the Difficulties with Emotion Regulation Scale (DERS()), was negatively correlated with left inferior frontal gyrus (IFG()) recruitment on trials assessing regulatory ability. These findings suggest that, within a sample of individuals with BPD, greater bottom-up amygdala activity is associated with heightened affective lability. By contrast, difficulties with emotion regulation are related to reduced IFG recruitment during emotion regulation. These results point to distinct neural mechanisms for different aspects of BPD symptomology

Multi-Imaging Method to Assay the Contractile Mechanical Output of Micropatterned Human iPSC-Derived Cardiac Myocytes.

RationaleDuring each beat, cardiac myocytes (CMs) generate the mechanical output necessary for heart function through contractile mechanisms that involve shortening of sarcomeres along myofibrils. Human-induced pluripotent stem cells (hiPSCs) can be differentiated into CMs (hiPSC-CMs) that model cardiac contractile mechanical output more robustly when micropatterned into physiological shapes. Quantifying the mechanical output of these cells enables us to assay cardiac activity in a dish.ObjectiveWe sought to develop a computational platform that integrates analytic approaches to quantify the mechanical output of single micropatterned hiPSC-CMs from microscopy videos.Methods and resultsWe micropatterned single hiPSC-CMs on deformable polyacrylamide substrates containing fluorescent microbeads. We acquired videos of single beating cells, of microbead displacement during contractions, and of fluorescently labeled myofibrils. These videos were independently analyzed to obtain parameters that capture the mechanical output of the imaged single cells. We also developed novel methods to quantify sarcomere length from videos of moving myofibrils and to analyze loss of synchronicity of beating in cells with contractile defects. We tested this computational platform by detecting variations in mechanical output induced by drugs and in cells expressing low levels of myosin-binding protein C.ConclusionsOur method can measure the cardiac function of single micropatterned hiPSC-CMs and determine contractile parameters that can be used to elucidate mechanisms that underlie variations in CM function. This platform will be amenable to future studies of the effects of mutations and drugs on cardiac function

Multi-Imaging Method to Assay the Contractile Mechanical Output of Micropatterned Human iPSC-Derived Cardiac Myocytes.

RationaleDuring each beat, cardiac myocytes (CMs) generate the mechanical output necessary for heart function through contractile mechanisms that involve shortening of sarcomeres along myofibrils. Human-induced pluripotent stem cells (hiPSCs) can be differentiated into CMs (hiPSC-CMs) that model cardiac contractile mechanical output more robustly when micropatterned into physiological shapes. Quantifying the mechanical output of these cells enables us to assay cardiac activity in a dish.ObjectiveWe sought to develop a computational platform that integrates analytic approaches to quantify the mechanical output of single micropatterned hiPSC-CMs from microscopy videos.Methods and resultsWe micropatterned single hiPSC-CMs on deformable polyacrylamide substrates containing fluorescent microbeads. We acquired videos of single beating cells, of microbead displacement during contractions, and of fluorescently labeled myofibrils. These videos were independently analyzed to obtain parameters that capture the mechanical output of the imaged single cells. We also developed novel methods to quantify sarcomere length from videos of moving myofibrils and to analyze loss of synchronicity of beating in cells with contractile defects. We tested this computational platform by detecting variations in mechanical output induced by drugs and in cells expressing low levels of myosin-binding protein C.ConclusionsOur method can measure the cardiac function of single micropatterned hiPSC-CMs and determine contractile parameters that can be used to elucidate mechanisms that underlie variations in CM function. This platform will be amenable to future studies of the effects of mutations and drugs on cardiac function