Characterization of 13 multi-drug resistant Salmonella serovars from different broiler chickens associated with those of human isolates

<p>Abstract</p> <p>Background</p> <p><it>Salmonella </it>are frequently isolated from chickens and their products. Prevalent serogroups and serovars of <it>Salmonella </it>as well as their genotypes and antibiograms were determined for cloacal samples from 1595 chickens. To understand the possible serovar and H antigens for transmission between chicken and human, serovars and their H antigens of 164 chicken and 5314 human isolates were compared.</p> <p>Results</p> <p>Prevalence of <it>Salmonella </it>differed among chicken lines and ages. Chicken and human isolates belonged mainly to serogroup B, C1, C2-C3, D, and E. 13 serovars and 66 serovars were identified for chicken and human isolates respectively. The common serovars for chicken and human isolates were <it>S</it>. Typhimurium, <it>S</it>. Enteritidis, <it>S</it>. Albany, <it>S</it>. Derby, and <it>S</it>. Anatum and shared common H1 antigens "g complex; i; e,h; and z4,z24" and H2 antigens "1 complex and -". In human isolates, H1 antigen "i" and H2 antigen "-" were common in all serogroups. In chicken, antimicrobial susceptibility differed among serogroups, serovars and three counties. All isolates were susceptible to cefazolin and ceftriaxone, but highly resistant to ampicillin, chloramphenicol, flumequine, streptomycin, sulfamethoxazole-trimethoprim, and tetracycline. Except those isolates of serogroup C1 of Chick group and serogroup G, all isolates were multi-drug resistance. Only <it>S</it>. Kubacha, <it>S</it>. Typhimurium, <it>S</it>. Grampian, and <it>S</it>. Mons were resistant to ciprofloxacin and/or enrofloxacin.</p> <p>Conclusion</p> <p>In chicken, prevalent serogroups and serovars were associated with chicken ages, lines and regions; and flouroquinolone-resistant and MDR isolates emerged. H1 antigens "g complex and i" and H2 antigens "1 complex and -" might be important for transmission of <it>Salmonella </it>between chicken and human.</p

Nano Fe3O4-activated carbon composites for aqueous supercapacitors

In this study, a symmetric supercapacitor has been fabricated by adopting the nanostructured iron oxide (Fe3O4)-activated carbon (AC) composite as the core electrode materials. The composite electrodes were prepared via a facile mechanical mixing process and PTFE polymeric solution has been used as the electrode material binder. Structural analysis of the nanocomposite electrodes were characterized by scanning electron microscopy (SEM) and Brunauer-Emmett-Teller (BET) analysis. The electrochemical performances of the prepared supercapacitor were studied using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in 1.0 M Na2SO3 and 1.0 M Na2SO4 aqueous solutions, respectively. The experimental results showed that the highest specific capacitance of 43 F/g is achieved with a fairly low Fe3O4 nanomaterials loading (4 wt. %) in 1 M Na2SO3. It is clear that the low concentration of nanostructured Fe3O4 has improved the capacitive performance of the composite via pseudocapacitance charge storage mechanism as well as the enhancement on the specific surface areas of the electrode. However, further increasing of the Fe3O4 content in the electrode is found to distort the capacitive performance and deteriorate the specific surface area of the electrode, mainly due to the aggregation of the Fe3O4 particles within the composite. Additionally, the CV results showed that the Fe3O4/AC nanocomposite electrode in Na2SO3 electrolyte exhibits a better charge storage performance if compared with Na2SO4 solution. It is believed that Fe3O4 nanoparticles can provide favourable surface adsorption sites for sulphite (SO32-) anions which act as catalysts for subsequent redox and intercalation reactions

Immunolocalization of chloride transporters to gill epithelia of euryhaline teleosts with opposite salinity-induced Na+/K+-ATPase responses

Opposite patterns of branchial Na+/K+-ATPase (NKA) responses were found in euryhaline milkfish (Chanos chanos) and pufferfish (Tetraodon nigroviridis) upon salinity challenge. Because the electrochemical gradient established by NKA is thought to be the driving force for transcellular Cl- transport in fish gills, the aim of this study was to explore whether the differential patterns of NKA responses found in milkfish and pufferfish would lead to distinct distribution of Cl- transporters in their gill epithelial cells indicating different Cl- transport mechanisms. In this study, immunolocalization of various Cl- transport proteins, including Na+/K+/2Cl(-) cotransporter (NKCC), cystic fibrosis transmembrane conductance regulator (CFTR), anion exchanger 1 (AE1), and chloride channel 3 (ClC-3), were double stained with NKA, the basolateral marker of branchial mitochondrion-rich cells (MRCs), to reveal the localization of these transporter proteins in gill MRC of FW- or SW-acclimated milkfish and pufferfish. Confocal microscopic observations showed that the localization of these transport proteins in the gill MRCs of the two studied species were similar. However, the number of gill NKA-immunoreactive (IR) cells in milkfish and pufferfish exhibited to vary with environmental salinities. An increase in the number of NKA-IR cells should lead to the elevation of NKA activity in FW milkfish and SW pufferfish. Taken together, the opposite branchial NKA responses observed in milkfish and pufferfish upon salinity challenge could be attributed to alterations in the number of NKA-IR cells. Furthermore, the localization of these Cl- transporters in gill MRCs of the two studied species was identical. It depicted the two studied euryhaline species possess the similar Cl- transport mechanisms in gills

Study of Bc --> J/psi pi, etac pi decays with perturbative QCD approach

The Bc --> J/psi pi, etac pi decays are studied with the perturbative QCD approach. It is found that form factors and branching ratios are sensitive to the parameters w, v, f_J/psi and f_etac, where w and v are the parameters of the charmonium wave functions for Coulomb potential and harmonic oscillator potential, respectively, f_J/psi and f_etac are the decay constants of the J/psi and etac mesons, respectively. The large branching ratios and the clear signals of the final states make the Bc --> J/psi pi, etac pi decays to be the prospective channels for measurements at the hadron collidersComment: 21 pages, revtex

A reduction of unilateral ureteral obstruction-induced renal fibrosis by a therapy combining valsartan with aliskiren

Wu WP, Chang CH, Chiu YT, Ku CL, Wen MC, Shu KH, Wu MJ. A reduction of unilateral ureteral obstruction-induced renal fibrosis by a therapy combining valsartan with aliskiren. Am J Physiol Renal Physiol 299: F929-F941, 2010. First published August 4, 2010; doi:10.1152/ajprenal.00192.2010.-The protective effect of combination therapy with valsartan and aliskiren against renal fibrosis remains to be defined. This study was undertaken to examine the protective effects of the combination of valsartan and aliskiren against renal fibrosis induced by unilateral ureteral obstruction (UUO). Combination therapy with valsartan (15 mg . kg(-1) . day(-1)) and aliskiren (10 mg . kg(-1) . day(-1)), valsartan monotherapy (30 mg . kg(-1) . day(-1)), and aliskiren monotherapy (20 mg . kg(-1) . day(-1)) all significantly ameliorated the increase in blood urea nitrogen and the degree of hydronephrosis determined by the increase in weight and length of the obstructed kidney. The dose titration study and blood pressure measurement confirmed that the combination therapy provided a greater benefit independent of the vasodilatory effect. There were no significant changes in serum levels of creatinine, sodium, and potassium in UUO rats and any treatment groups. Combination therapy also attenuated UUO-related increases in the scores of tubular dilatation, interstitial volume, interstitial collagen deposition, alpha-smooth muscle actin, the activation of ERK 1/2, the infiltration of monocytes/macrophages, the mRNA expression of snail-1, and transforming growth factor-beta 1 to a greater extent compared with aliskiren or valsartan used alone. The mRNA expression of renin and the (pro) renin receptor significantly increased after UUO. Combination therapy and monotherapy of valsartan and aliskiren had a comparable enhancing effect on the mRNA expression of renin, whereas all these treatments did not affect the expression of the (pro) renin receptor. In conclusion, a direct renin inhibitor in conjunction with an angiotensin II receptor blocker exerts increased renal protection against renal fibrosis and inflammation during obstruction over either agent alone

Semileptonic BcB_c decays and Charmonium distribution amplitude

In this paper we study the semileptonic decays of the $B_c$ meson in the Light-Cone Sum Rule (LCSR) approach. The result for each channel depends on the corresponding distribution amplitude of the final meson. For the case of $B_c$ decaying into a pseudoscalar meson, to twist-3 accuracy only the leading twist distribution amplitude (DA) is involved if we start from a chiral current. If we choose a suitable chiral current in the vector meson case, the main twist-3 contributions are also eliminated and we can consider the leading twist contribution only. The leading twist distribution amplitudes of the charmonium and other heavy mesons are given by a model approach in the reasonable way. Employing this charmonium distribution amplitude we find the cross section $\sigma(e^+e^-\to J/\psi+\eta_c)\simeq22.8 {fb}$ which is consistent with Belle and BaBar's data. Based on this model, we calculate the form factors for various $B_c$ decay modes in the corresponding regions. Extrapolating the form factors to the whole kinetic regions, we get the decay widths and branching ratios for various $B_c$ decay modes including their $\tau$ modes when they are kinematically accessible.Comment: Changed content partially, Added references, 16 pages,2 figure

Manipulation of electronic structure via supporting ligands: a charge disproportionate model within the linear metal framework of asymmetric nickel string Ni-7(phdptrany)(4)Cl (PF6)

This paper describes the synthesis and physical properties of an uniquely asymmetric heptanickel string complex exhibiting a charge disproportionate model along the linear nickel framework

Intracellular trafficking and replication of Burkholderia cenocepacia in human cystic fibrosis airway epithelial cells

We investigated the trafficking of Burkholderia cenocepacia , an opportunistic respiratory pathogen of persons with cystic fibrosis (CF), in immortalized CF airway epithelial cells in vitro . Our results indicate that bacteria enter cells in a process involving actin rearrangement. Whereas both live and heat-killed bacteria reside transiently in early endosomes, only live bacteria escape from late endosomes to colocalize in vesicles positive for lysosomal membrane marker LAMP1, endoplasmic reticulum (ER) membrane marker calnexin, and autophagosome marker monodansylcadavarine (MDC). Twenty-four hours after infection, microcolonies of live bacteria were observed in the perinuclear area colocalizing with calnexin. In contrast, after ingestion, dead bacteria colocalized with late endosome marker Rab7, and lysosome markers LAMP1 and cathepsin D, but not with calnexin or MDC. Six to eight hours after ingestion of dead bacteria, degraded bacterial particles were observed in the cytoplasm and in vesicles positive for cathepsin D. These results indicate that live B. cenocepacia gain entry into human CF airway cells by endocytosis, escape from late endosomes to enter autophagosomes that fail to fuse with mature lysosomes, and undergo replication in the ER. This survival and replication strategy may contribute to the capacity of B. cenocepacia to persist in the lungs of infected CF patients.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75744/1/j.1462-5822.2006.00724.x.pd

Heavy quarkonium: progress, puzzles, and opportunities

A golden age for heavy quarkonium physics dawned a decade ago, initiated by the confluence of exciting advances in quantum chromodynamics (QCD) and an explosion of related experimental activity. The early years of this period were chronicled in the Quarkonium Working Group (QWG) CERN Yellow Report (YR) in 2004, which presented a comprehensive review of the status of the field at that time and provided specific recommendations for further progress. However, the broad spectrum of subsequent breakthroughs, surprises, and continuing puzzles could only be partially anticipated. Since the release of the YR, the BESII program concluded only to give birth to BESIII; the $B$-factories and CLEO-c flourished; quarkonium production and polarization measurements at HERA and the Tevatron matured; and heavy-ion collisions at RHIC have opened a window on the deconfinement regime. All these experiments leave legacies of quality, precision, and unsolved mysteries for quarkonium physics, and therefore beg for continuing investigations. The plethora of newly-found quarkonium-like states unleashed a flood of theoretical investigations into new forms of matter such as quark-gluon hybrids, mesonic molecules, and tetraquarks. Measurements of the spectroscopy, decays, production, and in-medium behavior of c\bar{c}, b\bar{b}, and b\bar{c} bound states have been shown to validate some theoretical approaches to QCD and highlight lack of quantitative success for others. The intriguing details of quarkonium suppression in heavy-ion collisions that have emerged from RHIC have elevated the importance of separating hot- and cold-nuclear-matter effects in quark-gluon plasma studies. This review systematically addresses all these matters and concludes by prioritizing directions for ongoing and future efforts.Comment: 182 pages, 112 figures. Editors: N. Brambilla, S. Eidelman, B. K. Heltsley, R. Vogt. Section Coordinators: G. T. Bodwin, E. Eichten, A. D. Frawley, A. B. Meyer, R. E. Mitchell, V. Papadimitriou, P. Petreczky, A. A. Petrov, P. Robbe, A. Vair

Heavy Quarks and Heavy Quarkonia as Tests of Thermalization

We present here a brief summary of new results on heavy quarks and heavy quarkonia from the PHENIX experiment as presented at the "Quark Gluon Plasma Thermalization" Workshop in Vienna, Austria in August 2005, directly following the International Quark Matter Conference in Hungary.Comment: 8 pages, 5 figures, Quark Gluon Plasma Thermalization Workshop (Vienna August 2005) Proceeding