The usefulness of c-Kit in the immunohistochemical assessment of melanocytic lesions

C-Kit (CD117), the receptor for the stem cell factor, a growth factor for melanocyte migra- tion and proliferation, has shown differential immunostaining in various benign and malig- nant melanocytic lesions. The purpose of this study is to compare c-Kit immunostaining in benign nevi and in primary and metastatic malignant melanomas, to determine whether c-Kit can aid in the differential diagnosis of these lesions. c-Kit immunostaining was per- formed in 60 cases of pigmented lesions, including 39 benign nevi (5 blue nevi, 5 intra- dermal nevi, 3 junctional nevi, 15 cases of pri- mary compound nevus, 11 cases of Spitz nevus), 18 cases of primary malignant melanoma and 3 cases of metastatic melanoma. The vast majority of nevi and melanomas examined in this study were posi- tive for c-Kit, with minimal differences between benign and malignant lesions. C-Kit cytoplasmatic immunoreactivity in the intraepidermal proliferating nevus cells, was detected in benign pigmented lesions as well as in malignant melanoma, increasing with the age of patients (P=0.007) in both groups. The patient’s age at presentation appeared to be the variable able to cluster benign and malignant pigmented lesions. The percentage of c-Kit positive intraepidermal nevus cells was better associated with age despite other vari- ables (P=0.014). The intensity and percentage of c-Kit positivity in the proliferating nevus cells in the dermis was significantly increased in malignant melanocytic lesions (P=0.015 and P=0.008) compared to benign lesions (compound melanocytic nevi, Spitz nevi, intradermal nevi, blue nevi). Immunostaning for c-Kit in metastatic melanomas was nega- tive. Interestingly in two cases of melanoma occurring on a pre-existent nevus, the melanoma tumor cells showed strong cytoplas- matic and membranous positivity for c-kit, in contrast with the absence of any immunoreac- tivity in pre-existent intradermal nevus cells. C-Kit does not appear to be a strong immuno- histochemical marker for distinguishing melanoma from melanocytic nevi, if we consid- er c-Kit expression in intraepidermal prolifer- ating cells. The c-Kit expression in proliferat- ing melanocytes in the dermis could help in the differential diagnosis between a superfi- cial spreading melanoma (with dermis inva- sion) and a compound nevus or an intradermal nevus. Finally, c-Kit could be a good diagnostic tool for distinguishing benign compound nevi from malignant melanocytic lesions with der- mis invasion and to differentiate metastatic melanoma from primary melanoma

Oxidation behaviour of tungsten with vanadium additions

The effect of vanadium additions on the oxidation behaviour of tungsten has been evaluated at 600 °C in dry air. Mass gain in V-containing tungsten alloys is practically the same than that of pure tungsten processed by a conventional route. The advantage of vanadium alloying arises from the suppression of periodical microcracking described during the oxidation of pure tungsten, although the scale developed during the initial stages is very porous and it is not an effective barrier for inward oxygen ingress. Further protection is conferred when an outermost dense WO3 layer is formed at the air/scale interface by condensation of volatile W and V oxides, turning the kinetics from linear to parabolic

In-depth description of Electrohydrodynamic conduction pumping of dielectric liquids: physical model and regime analysis

In this work, we discuss the fundamental aspects of Electrohydrodynamic (EHD) conduction pumping of dielectric liquids. We build a mathematical model of conduction pumping that can be applied to all sizes, down to microsized pumps. In order to do this, we discuss the relevance of the Electrical Double Layer (EDL) that appears naturally on nonmetallic substrates. In the process, we identify a new dimensionless parameter related to the value of the zeta potential of the substrate-liquid pair, which quantifies the influence of these EDLs on the performance of the pump. This parameter also describes the transition from EHD conduction pumping to electro-osmosis. We also discuss in detail the two limiting working regimes in EHD conduction pumping: ohmic and saturation. We introduce a new dimensionless parameter, accounting for the electric field enhanced dissociation that, along with the conduction number, allows us to identify in which regime the pump operates.Ministerio de Ciencia, Innovación y Universidades PGC2018-099217-B-I0

Carbon-fiber tips for scanning probe microscopes and molecular electronics experiments

We fabricate and characterize carbon-fiber tips for their use in combined scanning tunneling and force microscopy based on piezoelectric quartz tuning fork force sensors. An electrochemical fabrication procedure to etch the tips is used to yield reproducible sub-100-nm apex. We also study electron transport through single-molecule junctions formed by a single octanethiol molecule bonded by the thiol anchoring group to a gold electrode and linked to a carbon tip by the methyl group. We observe the presence of conductance plateaus during the stretching of the molecular bridge, which is the signature of the formation of a molecular junction.Comment: Conference Proceeding (Trends in NanoTechnology 2011, Tenerife SPAIN); Nanoscale Research Letters, (2012) 7:25

Quantitatively probing propensity for structural transitions in engineered virus nanoparticles by single-molecule mechanical analysis

Viruses are increasingly being studied from the perspective of fundamental physics at the nanoscale as biologically evolved nanodevices with many technological applications. In viral particles of the minute virus of mice (MVM), folded segments of the single-stranded DNA genome are bound to the capsid inner wall and act as molecular buttresses that increase locally the mechanical stiffness of the particle. We have explored whether a quantitative linkage exists in MVM particles between their DNA-mediated stiffening and impairment of a heat-induced, virus-inactivating structural change. A series of structurally modified virus particles with disrupted capsid-DNA interactions and/or distorted capsid cavities close to the DNA-binding sites were engineered and characterized, both in classic kinetics assays and by single-molecule mechanical analysis using atomic force microscopy. The rate constant of the virus inactivation reaction was found to decrease exponentially with the increase in elastic constant (stiffness) of the regions closer to DNA-binding sites. The application of transition state theory suggests that the height of the free energy barrier of the virus-inactivating structural transition increases linearly with local mechanical stiffness. From a virological perspective, the results indicate that infectious MVM particles may have acquired the biological advantage of increased survival under thermal stress by evolving architectural elements that rigidify the particle and impair non-productive structural changes. From a nanotechnological perspective, this study provides proof of principle that determination of mechanical stiffness and its manipulation by protein engineering may be applied for quantitatively probing and tuning the conformational dynamics of virus-based and other protein-based nanoassemblies. This journal isThis work was funded by grants to M.G.M. from the Spanish Government (BIO2009-10092 and BIO2012-37649) and Comunidad de Madrid (S-505/MAT-0303) and by an institutional grant from Fundación Ramón Areces to the Centro de Biología Molecular. P.J.P.C. is the recipient of a FPI contract from the Spanish Government. M.G.M. is an associate member of the Institute for Biocomputation and Physics of Complex Systems, Zaragoza, Spain.Peer Reviewe

Breaking & Entering : PAH photodissociation and top-down chemistry

Laboratory, observation and modeling work on the dissociation of polycyclic aromatic hydrocarbons in interstellar environments and the formation of new molecular species through the fragmentation process.Laboratory astrophysics and astrochemistr

Seed germination and seedling allogamy in Rosmarinus officinalis: the costs of inbreeding

1) Self-pollination by geitonogamy is likely in self-compatible plants that simultaneously expose large numbers of flowers to pollinators. However, the progeny of these plants is often highly allogamous. Although mechanisms to increase cross-pollination have been identified and studied, their relative importance has rarely been addressed simultaneously in plant populations. (2) We used Rosmarinus officinalis to explore the factors that influence the probability of self-fertilization due to geitonogamy or that purge its consequences, focusing on their effect on seed germination and allogamy rate. For doing this, we experimentally tested the effects of geitonogamy on the proportion of filled seeds and how it influences germination rates. Then during two field seasons, we studied how life-history and flowering traits of individuals influence seed germination and allogamy rates of their progeny in wild populations at the extremes of the altitudinal range. The traits considered were plant size, population density, duration of the flowering season, number of open flowers, flowering synchrony among individuals within populations, and the proportion of male-sterile flowers. (3) We found that most seeds obtained experimentally from self-pollinations were apparently healthy but in fact empty, and that the presence of filled seeds drove the differences in germination rates between self- and cross-pollination experiments. Plants from wild populations consistently showed low germination rates and high rates of allogamy as determined with microsatellites. Germination rates related positively to the length of the flowering season, flowering synchrony and the rate of male-sterile flowers whereas the rate of allogamous seedlings was positively related only to the rate of male-sterile flowers. (4) Rosemary plants purge most of the inbreeding caused by its pollination system by aborting seeds. This study showed that the rates of seed germination and of the resulting allogamy are a function of a complex combination of factors that vary in space and time. Male sterility of flowers, length of the flowering season and flowering synchrony of individuals within populations all favor high rates of cross-pollination, therefore increasing germination and allogamy rates. These flowering traits appear to be highly plastic and respond to local and seasonal environmental conditions