In-season training load quantification of one-, two- and three-game week schedules in a top European professional soccer team

Top European soccer teams that play in UEFA competitions often participate in one, two- or three-games per week. Therefore, it is necessary to ensure optimal match-day performance and full recovery. The aim of this study was to quantify internal and external training load (TL) within five microcycles: M1 and M2 - one-game weeks; M3 and M4 - two-game weeks; M5 - three-game week). Thirteen elite soccer players participated in this study. A global positioning system (GPS) was used to measure the total distance covered and distances of different exercise training zones (1-5), the session ratings of perceived exertion (s-RPE) scores and the amount of creatine kinase (CK) created during daily training sessions for the 2015-2016 in-season period. The data were analysed with respect to the number of days prior to a given match. The main results indicate that there was a significant difference in training intensity for zone 1 between M2 and M4 (4010.2 ± 103.5 and 4507.6 ± 133.0 m, respectively); a significant difference in training intensity for zone 3 between M1 and M5 (686.1 ± 42.8 and 801.2 ± 61.2 m, respectively); a significant difference in the duration of the training sessions and matches between M2 and M5 (69.2 ± 2.1 and 79.6 ± 2.3) and M3 and M5 (69.7 ± 1.0 and 79.6 ± 2.3); and finally, there was a significant difference in CK between M3 and M2 (325.5 ± 155.0 and 194.4 ± 48.9). Moreover, there was a significant decrease in TL in the last day prior to a match, for all microcycles and all variables. There was no significant difference with respect to s-RPE. This study provides the first report of daily external and internal TLs and weekly accumulated load (training sessions and match demands) during one, two, and three-game week schedules in a group of elite soccer players. Expected significant differences are found in daily and accumulated loads for within- and between-game schedules. A similar pattern is exhibited for one- and two-game week microcycles regarding the day before the match, which exhibits a decrease in all variables. Despite the different number of games played per week, TL remain similar between microcycles for zone 2 and 5, plus s-RPE.This project was supported by the National Funds through FCT—Portuguese Foundation for Science and Technology (UID/DTP/04045/2013) and the European Fund for Regional Development (FEDER) allocated by European Union through the COMPETE 2020 Programme (POCI-01-0145-FEDER-006969)—competitiveness and internationalization (POCI). The authors disclose funding received for this work from any of the following organizations: National Institutes of Health (NIH); Welcome Trust; Howard Hughes Medical Institute (HHMI); and other(s).info:eu-repo/semantics/publishedVersio

CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish

Since its first application for site-directed mutagenesis, the CRISPR-Cas9 system has revolutionized genome engineering. Here, we present a validated workflow for the generation of targeted genomic deletions in zebrafish, including the design, cloning, and synthesis of single-guide RNAs and Cas9 mRNA, followed by microinjection in zebrafish embryos and subsequent genotype screening for the establishment of a mutant line. The versatility and efficiency of this pipeline makes the generation of zebrafish models a widely used approach in functional genetics. For complete details on the use and execution of this protocol, please refer to Amorim et al. (2020).This work was supported by the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement no. ERC_2015_StG _ 680156 _ZPR). J.B. acknowledges Fundação para a Ciência e a Tecnologia (FCT) for an FCT Scientific Stimulus Grant ( CEECIND/03482/2018 ). J.A. is a PhD fellow from FCT ( SFRH/BD/145110/2019 ). R.B.C. was funded by FCT ( ON2201403-CTO-BPD ) and EMBO (Short-Term Fellowship). We thank Joaquin Letelier, Ana Novoa, and Moises Mallo for important advice establishing the current protocol. We also thank Joana Marques for constant assistance in establishing the protocol

Proyecto OTALEX C (Observatorio Territorial y Ambiental Alentejo-Extremadura-Centro)

Comunicação oral da qual só está disponível o resumo.Proyecto OTALEX C (Observatorio Territorial y Ambiental Alentejo-Extremadura-Centro)

Acolhimento de refugiados: alimentação e necessidades nutricionais em situações de emergência

A Europa é neste momento um dos principais destinos de um intenso fluxo migratórioprovocado por diferentes conflitos armados no Médio Oriente e em África, tendo a ComissãoEuropeia (CE) acordado na distribuição de uma parte destas pessoas em clara necessidade deproteção nacional, pelos diversos Estados Membros. As populações em trânsito e ascaracterísticas do seu acolhimento possuem especificidades que podem comprometer oacesso a uma alimentação adequada e a cuidados básicos de saúde, influenciando amorbilidade e a mortalidade nos grupos afetados.Este manual pretende estabelecer um referencial para a intervenção alimentar e nutricionalaos refugiados que chegam a Portugal. Destina-se a todos aqueles que prestam apoio, quer anível individual, quer a nível institucional, e que sejam responsáveis por qualquer aspetorelacionado com a saúde e a alimentação dessas populações, facilitando a operacionalizaçãoda assistência e fornecendo ferramentas para a tomada de decisões.O manual está organizado em 3 partes. Inicia-se com a avaliação do estado nutricional dapopulação a acolher, apresenta depois diversas estratégias para o desenho da intervençãoalimentar e nutricional tendo por base as necessidades nutricionais previstas para estes grupospopulacionais e, na parte final, aborda a importância de garantir a higiene e segurança dosalimentos na prestação desta assistência alimentar. Este manual apresenta ainda algumasconsiderações relacionadas com os cuidados básicos na área da psicologia destinados àsequipas que estão no terrenoEurope is now a major destination of an intense migratory flow caused by armed conflicts inthe Middle East and Africa. In this sense, the European Commission (EC) agreed on thedistribution of some of these people in clear need of international protection by variousMember States. The populations in transit and the characteristics of its reception havespecificities that can compromise the access to adequate food and basic health care. They canalso influence morbidity and mortality in affected groups.This manual aims to establish a framework of nutrition and food intervention to refugees inPortugal. It is aimed at all those who support it, either individually or institutionally, and areresponsible for any aspect related to the health and nutrition of these populations, facilitatingthe implementation of care and providing tools for decision making. The manual is organizedinto 3 parts. It begins with the assessment of nutritional status of the population. Then presents several strategies to support the design of food and nutrition intervention based onthe estimated nutritional needs for these population groups. In the final section it addressesthe importance of ensuring food safety and hygiene when providing this food assistance. Thismanual also addresses some considerations towards basic psychological interventionprinciples, destined to help the teams on the field

Indicadores Ambientais da área OTALEX C

A área OTALEX C – Alentejo-Extremadura-Centro, está a ser caracterizada e monitorizada através de indicadores que se encontram enquadrados sistematicamente através de vetores e temas. Os indicadores permitem ilustrar e comunicar fenómenos complexos de forma mais simples e explicar tendências e evoluções desses mesmos fenómenos ao longo do tempo, possibilitando assim a monitorização de determinada área

A new species of Neocordulia Selys, 1882 (Odonata: Corduliidae) from Minas Gerais State, Brazil

Abstract: A new species of Neocordulia is described and illustrated based on a reared male, collected at Cachoeira da Eubisose stream, São Tomé das Letras, Minas Gerais State, Brazil. The Holotype is deposited in the Museu Nacional, UFRJ, Rio de Janeiro, Brazil