Caracterização de linhagens de soja Glycine max (L.) Merrill para produção de brotos.

Orientadoras: Juliana Steffens, Clarice Steffens e Mercedes Concórdia Carrão-Panizzi

Looking for imprints of the first stellar generations in metal-poor bulge field stars

© 2016 ESO. Context. Efforts to look for signatures of the first stars have concentrated on metal-poor halo objects. However, the low end of the bulge metallicity distribution has been shown to host some of the oldest objects in the Milky Way and hence this Galactic component potentially offers interesting targets to look at imprints of the first stellar generations. As a pilot project, we selected bulge field stars already identified in the ARGOS survey as having [Fe/H] 1 and oversolar [α/Fe] ratios, and we used FLAMES-UVES to obtain detailed abundances of key elements that are believed to reveal imprints of the first stellar generations. Aims. The main purpose of this study is to analyse selected ARGOS stars using new high-resolution (R ∼ 45 000) and high-signal-tonoise (S=N > 100) spectra. We aim to derive their stellar parameters and elemental ratios, in particular the abundances of C, N, the α-elements O, Mg, Si, Ca, and Ti, the odd-Z elements Na and Al, the neutron-capture s-process dominated elements Y, Zr, La, and Ba, and the r-element Eu. Methods. High-resolution spectra of five field giant stars were obtained at the 8 m VLT UT2-Kueyen telescope with the UVES spectrograph in FLAMES-UVES configuration. Spectroscopic parameters were derived based on the excitation and ionization equilibrium of Fe i and Fe ii. The abundance analysis was performed with a MARCS LTE spherical model atmosphere grid and the Turbospectrum spectrum synthesis code. Results.We confirm that the analysed stars are moderately metal-poor (-1:04≤[Fe/H]≤-0:43), non-carbon-enhanced (non-CEMP) with [C/Fe] ≤+0:2, and α-enhanced.We find that our three most metal-poor stars are nitrogen enhanced. The α-enhancement suggests that these stars were formed from a gas enriched by core-collapse supernovae, and that the values are in agreement with results in the literature for bulge stars in the same metallicity range. No abundance anomalies (Na-O, Al-O, Al-Mg anti-correlations) were detected in our sample. The heavy elements Y, Zr, Ba, La, and Eu also exhibit oversolar abundances. Three out of the five stars analysed here show slightly enhanced [Y/Ba] ratios similar to those found in other metal-poor bulge globular clusters (NGC 6522 and M 62). Conclusions. This sample shows enhancement in the first-to-second peak abundance ratios of heavy elements, as well as dominantly s-process element excesses. This can be explained by different nucleosynthesis scenarios: (a) the main r-process plus extra mechanisms, such as the weak r-process; (b) mass transfer from asymptotic giant branch stars in binary systems; (c) an early generation of fast-rotating massive stars. Larger samples of moderately metal-poor bulge stars, with detailed chemical abundances, are needed to better constrain the source of dominantly s-process elements in the early Universe

Efeito do tebuconazol no comportamento de fuga de Eisenia andrei em ensaios de laboratórios com solo natural.

FertBio 2010

Fauna edáfica como indicadora de contaminação do solo.

Recentemente, o solo tem se tornado foco de preocupação devido à gradativa contaminação de ambientes terrestres e aquáticos, decorrente do progresso e avanços sócio-econômicos no país. Diante dessa realidade, a demanda por atividades antrópicas menos agressivas ao ambiente é cada vez maior. Os possíveis impactos ambientais, portanto, devem ser monitorados, controlados e remediados, a fim de evitar problemas irreversíveis ao meio ambiente e à sociedade. A ecotoxicologia estuda os efeitos dos poluentes sobre os organismos e a interação destes com o habitat. Para se avaliar o impacto de uma substância no solo, ensaios ecotoxicológicos com metodologia padronizada internacionalmente podem ser realizados com invertebrados edáficos, tais como as minhocas, enquitreídeos e colêmbolos, por serem importantes na decomposição da matéria orgânica do solo. Esses ensaios de laboratório, no entanto, precisam ser adaptados, pois a metodologia padrão se baseia em espécies e condições de clima temperado que não condizem com a realidade do Brasil. Adaptações aos ensaios de efeito agudo (mortalidade), efeito crônico (reprodução) e de fuga (comportamento) têm sido estudados por vários grupos e os resultados têm sido positivos com relação ao uso do substrato com o pó da fibra da casca do côco e utilização de temperaturas maiores que 20ºC. Algumas espécies encontradas no Brasil também têm sido testadas, algumas delas apresentando resultados promissores, outras, com limitações. De modo geral, os métodos para avaliação da contaminação do solo em laboratório têm apresentado avanços, entretanto, mais estudos se fazem necessários para o estabelecimento de espécies nativas recomendadas para cada tipo de ensaio.Resumo expandido

Improvement in soybean sprouts production with ultrasound power.

The present work aimed the application of different ultrasound powers (10, 50 and 90W) to evaluate the germination and characteristics of soybean sprouts fromsoybeans of cultivar BRS 216 and 3 lines (A, B and C). In the germination test,the line A showed the highest percentage of germination (96% for 90W).The cultivar BRS 216 and line A present lower values of weight and higher yield of sprouts. The viable sprouts that presented the highest weight (for each 50g of soybean) were: line A followed by BRS 216, line C and B, with statistical differences (p<0.05) between them. For each 50g of soybean of cultivar BRS 216 and Lines A, B and C the production of viable sprouts was 231.47; 237.87; 170.74 and 199.94g, respectively, for 90W. In relation to the length of the sprouts using the power of 10W the line C presented a longer length. The line A using 90W showed better color characteristics. The highest protein value was found in cultivar BRS 216, for all applied potencies. With this work it can be concluded that the line A would be the most suitable for sprout production regardless of the ultrasound power applie

Duodenal-jejunal Bypass Normalizes Pancreatic Islet Proliferation Rate And Function But Not Hepatic Steatosis In Hypothalamic Obese Rats

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Modifications in life-style and/or pharmacotherapies contribute to weight loss and ameliorate the metabolic profile of dietinduced obese humans and rodents. Since these strategies fail to treat hypothalamic obesity, we have assessed the possible mechanisms by which duodenal-jejunal bypass (DJB) surgery regulates hepatic lipid metabolism and the morphophysiology of pancreatic islets, in hypothalamic obese (HyO) rats. During the first 5 days of life, male Wistar rats received subcutaneous injections of monosodium glutamate (4 g/kg body weight, HyO group), or saline (CTL). At 90 days of age, HyO rats were randomly subjected to DJB (HyO DJB group) or sham surgery (HyO Sham group). HyO Sham rats were morbidly obese, insulin resistant, hypertriglyceridemic and displayed higher serum concentrations of non-esterified fatty acids (NEFA) and hepatic triglyceride (TG). These effects were associated with higher expressions of the lipogenic genes and fatty acid synthase (FASN) protein content in the liver. Furthermore, hepatic genes involved in beta-oxidation and TG export were down-regulated in HyO rats. In addition, these rats exhibited hyperinsulinemia, beta-cell hypersecretion, a higher percentage of islets and beta-cell area/pancreas section, and enhanced nuclear content of Ki67 protein in islet-cells. At 2 months after DJB surgery, serum concentrations of TG and NEFA, but not hepatic TG accumulation and gene and protein expressions, were normalized in HyO rats. Insulin release and Ki67 positive cells were also normalized in HyO DJB islets. In conclusion, DJB decreased islet-cell proliferation, normalized insulinemia, and ameliorated insulin sensitivity and plasma lipid profile, independently of changes in hepatic metabolism.505Fundacao Araucaria [155/2013, 393/2013]Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2015/12611-0]Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Anelastic spectroscopy study of the spin-glass and cluster spin-glass phases of La2−x_{2-x}Srx_{x}CuO4_{4} (0.015<x<0.03)(0.015<x<0.03)

The anelastic spectra of La$_{2-x}$Sr$_{x}$CuO$_{4}$ have been measured at liquid He temperatures slightly below and above the concentration $x_{c}\simeq 0.02$ which is considered to separate the spin-glass phase from the cluster spin-glass (CSG) phase. For $x\le x_{c}$ all the elastic energy loss functions show a step below the temperature $T_{g}(x=0.02)$ of freezing into the CSG state, similarly to what found in samples well within the CSG phase, but with a smaller amplitude. The excess dissipation in the CSG state is attributed to the motion of the domain walls between the clusters of antiferromagnetically correlated spin. These results are in agreement with the recent proposal, based on inelastic neutron scattering, of an electronic phase separation between regions with $x\sim 0$ and $x\sim 0.02$, at least for $x>0.015$Comment: 5 pages, 3 figures, submitted to Phys. Rev.

Evaluation of packaging systems with O2-absorbers on quality of minimally processed soybean sprouts.

Abstract: This study evaluated the influence of O2-absorbing sachets into different packages polyethylene terephthalate (PET ? E1), low density polyethylene (LDPE ? E2), and PET/LDPE (E3) on the quality of minimally processed soybean sprouts (MP-sprouts). The MP-sprouts were stored up to 12 days and characterized for physicochemical, microbiological and sensory attributes. The O2-absorbing sachet showed changes during exposure to the environment by the formation of amorphous iron hydroxide, which was observed by the analysis of functional groups and XRD. The packaging used in the study showed high transmittance and clarity, and low Haze, being a good feature for storing the MP product. All packaging systems showed an increase in the volume of absorbed oxygen (cm3) up to 9 days of storage. The physic-chemical characteristics of the MP-sprouts stored for 12 days were preserved in the different packages, showing no difference regarding the use of the O2-absorbing sachet. The O2-absorbing sachet not influenced the quality of MP-sprouts during the stored (12 days). The best visual aspect was observed in PET package (with and without O2-absorbing sachet) being classified as excellent, without color change. The MP-sprouts also fulfilled the microbiological quality standards and presented 75.11% acceptability and 72.40% purchase intention

A preclinical pipeline to evaluate migrastatics as therapeutic agents in metastatic melanoma

© The Author(s) 2021.[Background]: Metastasis is a hallmark of cancer and responsible for most cancer deaths. Migrastatics were defined as drugs interfering with all modes of cancer cell invasion and thus cancers’ ability to metastasise. First anti-metastatic treatments have recently been approved. [Methods]: We used bioinformatic analyses of publicly available melanoma databases. Experimentally, we performed in vitro target validation (including 2.5D cell morphology analysis and mass spectrometric analysis of RhoA binding partners), developed a new traceable spontaneously metastasising murine melanoma model for in vivo validation, and employed histology (haematoxylin/eosin and phospho-myosin II staining) to confirm drug action in harvested tumour tissues. [Results]: Unbiased and targeted bioinformatic analyses identified the Rho kinase (ROCK)-myosin II pathway and its various components as potentially relevant targets in melanoma. In vitro validation demonstrated redundancy of several RhoGEFs upstream of RhoA and confirmed ROCK as a druggable target downstream of RhoA. The anti-metastatic effects of two ROCK inhibitors were demonstrated through in vivo melanoma metastasis tracking and inhibitor effects also confirmed ex vivo by digital pathology. [Conclusions]: We proposed a migrastatic drug development pipeline. As part of the pipeline, we provide a new traceable spontaneous melanoma metastasis model for in vivo quantification of metastasis and anti-metastatic effects by non-invasive imaging.GOF’s lab was supported by Cancer Research UK [C48390/A21153], Worldwide Cancer Research [16-1153], and King’s Health Partners [King’s Medical Research Trust Joint Research Committee studentship to A.V.]. B.F. was supported by a King’s Health Partners studentship to V.S.M. and G.O.F. V.S.M.’s lab was supported by Cancer Research UK [C33043/A12065] and [C33043/A24478] (V.S.M., E.C.M., J.L.O., L.B. and GC), the Royal Society [RG110591] (V.S.M.), The Harry J. Lloyd Charitable Trust (J.L.O. and V.S.M.), the Barts Charity (V.S.M., J.L.O., O.M., I.R.H. and E.C.M.), the Fundacion Alfonso Martin Escudero and Marie Sklodowska-Curie Action [H2020-MSCA-IF-2014-EF-ST] (I.R.H.), and Fundacion Ramon Areces (E.C.M.). F.M. was supported by an MRC Career Development Award (MR/P009417/1). This work was further supported by the Department of Health (DoH) via the National Institute for Health Research (NIHR) Comprehensive Biomedical Research Centre award to King’s Health Partners, and the Wellcome/EPSRC Centre for Medical Engineering [WT203148/Z/16/Z]. Views expressed are those of the authors and not necessarily those of the NHS, NIHR or DoH

Sistema simplificado para produção de brotos de soja: uma atividade econômica para pequenos empreendimentos.

A soja é uma excelente fonte de nutrientes, com a proteína perfazen­do cerca de 40% da matéria seca, e com adequado perfil de aminoácidos, segundo os padrões da FAO. Outro componente importante é o óleo, que corresponde a cerca de 20% da matéria seca, com teor de ácidos graxos polinsaturados da ordem de 85%. Além disso, contém vários micronutrientes incluindo cálcio, ácido fólico e ferro. Entretanto, a soja se destaca por ser boa fonte de isoflavonas, as quais são benéficas para a saúde humana, e fazem da soja ingrediente para alimentos funcionais. Embora o uso de soja seja mais popular nos países do oriente, seu consumo vem crescendo nos países do ocidente, tanto em relação aos produtos tradicionais (farinha de soja, ?kinako?, tofu e ?leite?), como para os produtos processados que utilizam soja como ingrediente. O consumo de soja tem aumentado devido às técnicas adequadas de processamento que favorecem o sabor e a qualidade dos produtos alimentícios. Consumo direto de soja é tradicional em países asiáticos, os quais consomem extrato hidrossolúvel, tofu, grãos verdes ou imaturos (edamame) e brotos de soja, entre outros alimentos. (...)O objetivo do trabalho foi avaliar a viabilidade da tecnologia do processo automatizado de produção de brotos de soja da Embrapa, a partir da simplificação do equipamento desenvolvido por Oliveira e Carrão-Panizzi (2016a). A qualidade dos brotos obtidos com o sistema simplificado também foi verificada nas avaliações das características físico-químicas dos mesmos. A cultivar BRS 216, desenvolvida pela Embrapa para produção de brotos de soja, foi utilizada nesse estudo, pois apresenta sementes pequenas (10 g/peso de 100 sementes), característica essencial para produção de brotos. Palavras-chaves: soja, Glycine max (L.)Merrill, BRS 2016, produção de broto, alimentação humanabitstream/item/221446/1/ComTec-374-online.pd